scholarly journals Newly developed method for the determination of sitagliptin phosphate using rubeanic acid as a precipitating agent via the use of ISNAG continues flow fluorimeter

2020 ◽  
Vol 11 (3) ◽  
pp. 3613-3625
Author(s):  
Nagam S.Turkey Al-Awadie ◽  
Kefah H. Ismael Al-Saadi
Keyword(s):  
Classical Method ◽  
Standard Addition ◽  
Calibration Graph ◽  
Physical Parameters ◽  
Pharmaceutical Drugs ◽  
Significant Difference ◽  
Rubeanic Acid ◽  
Detector Cell ◽  
Method Analysis

A new, a simple, sensitive and fast developed method for the estimation of sitagliptin phosphate in pure and pharmaceutical drugs (tablet). This method based on the reaction between sitagliptin phosphate and rubeanic acid to form reddish-black ppt, using ISNAG-Fluorimeter analyzer via the measure of diverged light at 90° from radiation source which is used a range of high intensity ultraviolet 184.9 nm & 253.7 nm by low-pressure mercury lamp while detector cell can detect at 410-1150 nm. The resultant of diverged light by an interaction between incident photons and precipitated particles giving rise to a longer wavelength that the detector can detected. Chemical and physical parameters were studied and optimized. The calibration graph was linear in the range of (0.03-13 ) mmol.L−1 , with correlation coefficient ( r )= 0.9881 ,linearity percentage r2%= 97.64 , L.O.D = 0.7848µg/sample and RSD lower than 0.6 % for (3&7 ) mmol.L−1 ( eight replicates) concentration of sitagliptin phosphate. This method was successfully applied for the determination of sitagliptin phosphate in two different companies of pharmaceutical drugs. A comparison was made between the newly developed method analysis with the classical method (UV-spectrophotometer by measuring of absorbance ) using standard addition method via the use of t-test, it was noticed that there was no significant difference between two methods at %95 confidence level.

scholarly journals CFIA-Turbidimetric and Photometric Determination of Vitamin B9 (Folic acid) Using LEDs as a Source of Irradiation and Two Solar Cells as an Energy Transducer

2017 ◽  
Vol 14 (4) ◽  
pp. 773-786
Author(s):  
Baghdad Science Journal
Keyword(s):  
Folic Acid ◽  
Classical Method ◽  
Standard Addition ◽  
Photometric Determination ◽  
Calibration Graph ◽  
Ammonium Molybdate ◽  
Simple Method ◽  
Vitamin B9 ◽  
Significant Difference

A specific, sensitive and simple method was used for the determination of: vitamin B9 (Folic acid) in pure and pharmaceutical formulations using continuous flow injection analysis. The method is based on formation of ion pair compound between folic acid and ammonium molybdate in an aqueous medium to obtain a gray precipitate complex, using homemade; Ayah-6SX1-ST-2D solar cell CFI Analyzer. Optimum parameters was studied to increase the sensitivity for developed method. The linear range for the calibration graph was 0.01-0.6 mMol.L-1 of vitamin B9 and LOD was 131.994 ng/sample with correlation coefficient ( r ) of 0.9810, RSD% was lower than 0.1%, (n=9) for the determination of vitamin B9 at concentration (0.07and 0.5) mMol.L-1 respectively. The developed method was applied successfully for the determination of vitamin B9 in pharmaceutical tablets. A comparison was made between two methods: developed method and the classical UV spectrophotometric method at ?max=255 nm, by using the standard addition method via the use of paired t-test. It showed that there was no significant difference between the developed method and the classical method for determination vitamin B9 at 95% confidence level.

scholarly journals Assessment of long distance chasing photometer (NAG-ADF-300-2) by estimating the drug atenolol with ammonium molybdate via continuous flow injection analysis

2020 ◽  
Vol 17 (1) ◽  
pp. 0078
Author(s):  
Elham N. Mezaal N. Mezaal
Keyword(s):  
Flow Injection ◽  
Flow Injection Analysis ◽  
Continuous Flow ◽  
Classical Method ◽  
Standard Addition ◽  
Calibration Graph ◽  
Ammonium Molybdate ◽  
Long Distance ◽  
Optimum Parameters

     Atenolol was used with ammonium molybdate to prove the efficiency, reliability and repeatability of the long distance chasing photometer (NAG-ADF-300-2) using continuous flow injection analysis. The method is based on reaction between atenolol and ammonium molybdate in an aqueous medium to obtain a dark brown precipitate. Optimum parameters was studied to increase the sensitivity for developed method. A linear range for calibration graph was 0.1-3.5 mmol/L for cell A and 0.3-3.5 mmol/L for cell B, and LOD 133.1680 ng/100 µL and 532.6720 ng/100 µL for cell A and cell B respectively with correlation coefficient (r) 0.9910 for cell A and 0.9901 for cell B, RSD% was lower than 1%, (n=8) for the determination of atenolol at concentration (0.5, 0.7 and 5) mmol/L respectively. The results were compared with classical method UV-Spectrophotometric at λ max=270 nm using the standard addition method via the use of t-test, at 95% confidence level. The comparison of data explain that long distance chasing photometer (NAG-ADF-300-2) is the choice with excellent extended detection and wide application.                                                                                                       

scholarly journals New Fluorometric Method for the Determination of Ketotifen Fumarate Using Continuous Flow Injection Analysis via ISNAG-fluorimeter

2019 ◽  
Vol 16 (2) ◽  
pp. 0353
Author(s):  
Turkie Et al.
Keyword(s):  
Flow Injection ◽  
Limit Of Detection ◽  
Classical Method ◽  
Calibration Graph ◽  
Injection System ◽  
Minimum Concentration ◽  
Ketotifen Fumarate ◽  
Fluorescence Measurements ◽  
Significant Difference

        A newly developed analytical method was conducted for the determination of Ketotifen fumarate (KTF) in pharmaceuticals drugs via quenching of continuous fluorescence of 9(10H)-Acridone (ACD). The method was applied using flow injection system of a new homemade ISNAG fluorimeter with fluorescence measurements at ± 90◦ via 2×4 solar cell. The calibration graph was linear in the range of 1-45 mmol/L, with correlation coefficient r = 0.9762 and the limit of detection 29.785 µg/sample from the stepwise dilution for the minimum concentration in the linear dynamic ranged of the calibration graph. The method was successfully applied to the determination of Ketotifen fumarate in two different pharmaceutical drugs. A comparison was made between the newly developed method analysis and the classical method using the standard addition method via the use of individual and paired t-test and F-test. It was noticed that there was no significant difference between the two methods at 95 % confidence level.

scholarly journals New Fluorometric Method for the Determination of Ketotifen Fumarate Using Continuous Flow Injection Analysis via ISNAG-fluorimeter

2019 ◽  
Vol 16 (2) ◽  
pp. 0353
Author(s):  
Turkie Et al.
Keyword(s):  
Flow Injection ◽  
Limit Of Detection ◽  
Classical Method ◽  
Calibration Graph ◽  
Injection System ◽  
Minimum Concentration ◽  
Ketotifen Fumarate ◽  
Fluorescence Measurements ◽  
Significant Difference

        A newly developed analytical method was conducted for the determination of Ketotifen fumarate (KTF) in pharmaceuticals drugs via quenching of continuous fluorescence of 9(10H)-Acridone (ACD). The method was applied using flow injection system of a new homemade ISNAG fluorimeter with fluorescence measurements at ± 90◦ via 2×4 solar cell. The calibration graph was linear in the range of 1-45 mmol/L, with correlation coefficient r = 0.9762 and the limit of detection 29.785 µg/sample from the stepwise dilution for the minimum concentration in the linear dynamic ranged of the calibration graph. The method was successfully applied to the determination of Ketotifen fumarate in two different pharmaceutical drugs. A comparison was made between the newly developed method analysis and the classical method using the standard addition method via the use of individual and paired t-test and F-test. It was noticed that there was no significant difference between the two methods at 95 % confidence level.

scholarly journals New Method for the On-Line Determination of Molybdenum Via New Microphotometric Home Mode Instrument, Using Hight Emitting Diode as a Variable Intensity Radiant Source with a Miniture Photosilicone Diode Detector for System: Molybdenum(VI)-Hydrogen Peroxide-Ammonium Solution

2007 ◽  
Vol 4 (4) ◽  
pp. 571-582
Author(s):  
Baghdad Science Journal
Keyword(s):  
Hydrogen Peroxide ◽  
Classical Method ◽  
Wave Length ◽  
Research Work ◽  
Standard Addition ◽  
Ammonia Solution ◽  
Variable Intensity ◽  
Measurement Variation ◽  
On Line

This research work aims to the determination of molybdenum (VI) ion via the formation of peroxy molybdenum compounds which has red-brown colour with absorbance wave length at 455nm for the system of ammonia solution-hydrogen peroxide-molybdenum (VI) using a completely newly developed microphotometer based on the ON-Line measurement. Variation of responses expressed in millivolt. A correlation coefficient of 0.9925 for the range of 2.5-150 ?g.ml-1 with percentage linearity of 98.50%. A detection limit of 0.25 ?g.ml-1 was obtained. All physical and chemical variable were optimized interferences of cation and anion were studied classical method of measurement were done and compared well with newly on-line measurements. Application for the use of developed method on alloy samples, using standard addition method and agreed quite wall.

scholarly journals Greener Analytical Method for Determination of Iodine Number of Edible Oils

2020 ◽  
Vol 9 (6) ◽  
pp. 36
Author(s):  
Thidarat Kruatian ◽  
Kritsana Jitmanee
Keyword(s):  
Iodine Number ◽  
Standard Method ◽  
Analytical Method ◽  
Present Method ◽  
Edible Oils ◽  
Classical Method ◽  
Sample Weight ◽  
Number Range ◽  
Significant Difference

A greener analytical method for determination of iodine number (IN) of oils is presented. As per the AOAC standard method, a large amount of solvent and reagent was used, and long incubation time was required. This research is aimed at using less amount of solvent and reagent, less sample weight, and shorten the analysis time by using the modified titrimetric AOAC standard method. The study showed that by reducing the sample size, the amount of reagent could be decreased to 1.00 mL and the reaction time of 1 min is enough for completion of the reaction. The amount of reagent used was at least 25 times less than that of the classical method. There was no significant difference at 95% confidence level between the results obtained by the proposed method and the standard method, and both results correlated well. The present method can be applied to edible oils commonly found in the market (iodine number range of 6.0 to 130).

scholarly journals Rapid Determination of Neomycin by a Microbiological Agar Diffusion Assay Using Triphenyltetrazolium Chloride

1996 ◽  
Vol 79 (2) ◽  
pp. 434-440 ◽  
Author(s):  
Célia H Yamamoto ◽  
Terezinha J A Pinto
Keyword(s):  
Incubation Time ◽  
Rapid Determination ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Pharmaceutical Drugs ◽  
Triphenyltetrazolium Chloride ◽  
Agar Diffusion ◽  
Significant Difference ◽  
Physical Response

Abstract The standard, quantitative determination of neomycin activity by the agar diffusion method requires an 18 to 24 h incubation time. To reduce incubation time, an alternative method using triphenyltetra- zolium chloride has been developed. The inhibition zoneappears much earlier; hence, an effort was made to standardize it. This indicator develops a physical response, related to the biological one, after a determined period. A Staphylococcus epidermidis suspension inoculated into solid medium ata concentration low enough that growth is not visible can reduce the dye to formazan. There is enough contrast to read the inhibition zone optically after a7 h incubation. There is no significant difference between standard curves for 7 and 24 h incubations.Comparative assays for some pharmaceutical drugs containing neomycin in different forms show that it is possible to reduce incubation time. This modification is valid because no dispersion of results was detected by statistical analysis, which indicates that they belong to the same population. Thus, a rapid microbiological assay of neomycin has been validated and standardized

scholarly journals Of bromination reaction for the spectrophotometric assay of domperidone in pharmaceuticals

2011 ◽  
Vol 17 (1) ◽  
pp. 81-89 ◽  
Author(s):  
Zenita Devi ◽  
K. Basavaiah ◽  
K.B. Vinay
Keyword(s):  
Limit Of Detection ◽  
Pharmaceutical Preparations ◽  
Reference Method ◽  
Standard Addition ◽  
Limit Of Quantification ◽  
Fixed Amount ◽  
Spectrophotometric Methods ◽  
Significant Difference ◽  
Bulk Drug

Three simple and sensitive spectrophotometric methods are described for the determination of domperidone (DOM) in bulk drug and in dosage forms using bromate-bromide mixture as brominating agent in acid medium and three dyes, meta-cresol purple (MCP), amaranth (AMR) and erioglaucine (EGC). The methods involve the addition of a known excess of bromate-bromide mixture to an acidified solution of DOM followed by the determination of the residual bromine by reacting with a fixed amount of either MCP dye and measuring the absorbance at 530 nm (method A) or AMR dye and measuring the absorbance at 520 nm (method B) or EGC dye and measuring the absorbance at 630 nm (method C). Beer?s law is obeyed over the concentration ranges, 0.63 - 10.0, 0.25-4.0 and 0.13-2.0 ?g mL-1 for method A, method B and method C, respectively. The apparent molar absorptivities are calculated to be 3.751 ? 104, 6.604 ? 104 and 1.987 ? 105 L mol-1cm-1 for method A, method B and method C, respectively and the corresponding sandell sensitivity values are 0.011, 0.006 and 0.002 ?g cm-2. The limit of detection and the limit of quantification are also reported for all the three methods. No interference was observed from common additives found in pharmaceutical preparations. Statistical comparisons of the results with those of the reference method showed an excellent agreement, and indicated no significant difference in accuracy and precision. The accuracy and reliability of the methods were further ascertained by performing recovery tests via standard-addition technique.

Quantitative Determination of Arsenate in Dried Shrimp by Spectrophotometric Measurement of its Heteropoly Blue

2013 ◽  
Vol 705 ◽  
pp. 9-14
Author(s):  
Charuwan Suitcharit
Keyword(s):  
Complex Formation ◽  
Standard Addition ◽  
Cost Effective ◽  
Calibration Graph ◽  
Formation Condition ◽  
Blue Color ◽  
Molybdenum Blue ◽  
Effects Of Ph ◽  
Good Agreement

A cost-effective and sensitive spectrophotometric method for the determination of arsenate in dried shrimp has been developed using molybdenum blue as a chromogenic reagent. The method is based on arsenate conversion to arsenomolybdate heteropoly blue having an absorbance maximum at 870 nm. The effects of pH, time, temperature and light on its complex formation were investigated. The optimal complex formation condition at pH 4 in 90 min at 15+1°C was obtained and the blue color was favorable developed in daylight. The proposed method has been successfully applied for the determination of arsenate in samples with the addition of DTPA to eliminate the interferences resulting in increased selectivity. The standard addition calibration graph was constructed with the linear range extended to 40 μgL-1 (r2 = 0.9987). The detection limit (S/N = 3) of 3.21 μgL-1, and the precision of 3.13% (RSD) were obtained. The method has good recovery of 95% for the determination of arsenate. The amounts of arsenate in dried shrimp samples compared with those obtained from ICP-OES were shown to be in good agreement (r = 0.998).

scholarly journals The DETERMINATION OF AMILORIDE VIA QUENCHED CONTINUOUS FLUORESCENCE OF AZO DYE USING LOW-PRESSURE MERCURY LAMP TUBE (UV-LIGHT) AND MULTI SOLAR CELLS AT 2 X 90° AS A DETECTORS

2020 ◽  
pp. 211-218
Author(s):  
HUDA MUAYAD NAFEA ◽  
NAGHAM S. TURKEY
Keyword(s):  
Fluorescence Quenching ◽  
Azo Dye ◽  
Classical Method ◽  
Uv Light ◽  
Low Pressure ◽  
Pharmaceutical Drugs ◽  
Mercury Lamp ◽  
Fluorescence Measurements ◽  
Comparison Results

Objective: The aim of the method was to develop a novel, simple and rapid fluorometric method determination of Amiloride (AMD) in pure form and pharmaceutical drugs via fluorescence measurements. Methods: The method depends on fluorescence quenching of 2H-chromene azo dye (2-(4-nitrophenyl)-N-(4-(phenyldiazenyl)-2H-chromen-4-amine) upon adding Amiloride (AMD) using homemade ISNAG 2 X 90° multi solar cell via low-pressure mercury lamp at two significant wavelengths 184.9 and 253.7 nm combined with continuous flow injection analysis. Results: Under the optimized conditions, the fluorescence quenching linear working range and percentage linearity (r2%) was (0.03-8 mmol/l) and 98.78 %, respectively. The suggested method was effectively applied to the determination of AMD in two different pharmaceutical drugs and compared with the classical method (UV-vis spectrophotometry at λ=540 nm). Conclusion: The proposed and established method is simple, direct, and efficient. The statistical comparison results using a t-test at 95% confidence interval that was applied to compare the new and classical method showed there are no significant differences between the two methods.

Sign in / Sign up

Export Citation Format

Share Document