scholarly journals THE ADDITION OF FRESH GARLIC (Allium sativum L) IN DUCK FEED REDUCES CHOLESTEROL CONTENT OF DUCK EGG

2019 ◽  
Vol 12 (4) ◽  
Author(s):  
I Nyoman Sukartha Jaya ◽  
Ni Ketut Dewi Haryani ◽  
Budi Indarsih
Keyword(s):  
Total Cholesterol ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Egg Yolk ◽  
Cholesterol Content ◽  
Color Test ◽  
Treatment Groups ◽  
Egg Yolks ◽  
Duck Egg ◽  
Yolk Cholesterol

The objective of this study to determine the effect of feeding fresh garlic to ducks on decrease the cholesterol level of duck eggs. This study composed of two treatment groups. The duck in the first group (control) was fed local feed without spplemente d with fresh garlic, while in the second group was fed with local feed and supplemented with 50 grams of fresh garlic/duck/day. Egg yolk cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and triglycerides (TG) analysis were carried out at the end of the study using the ether extract method and then with the Cholesterol Oksidase Para Amino Penazone (CHOD-PAP) or enzymatic color test. Data was analysed using one way Analysis of Variance (ANOVA) the results showed that fresh garlic reduced total cholesterol, LDL fraction, and triglyceride, but increased the HDL fraction of duck egg yolk. The total cholesterol, LDL fraction, and triglyceride of duck egg yolks dropped by around 15.98 mg per dL (8.06%), 10.40 mg per dL (12.97%), 64.92 mg per dL (12.71%), respectively, while the HDL fraction increased by 11.40 mg per dL (19.32%) The results of this study indicated that fresh garlic can be used to produce specific products such as low cholesterol and low fat duck eggs.

scholarly journals Effect of Alternative Housing System and Hen Genotype on Egg Quality Characteristics

2018 ◽  
pp. 695 ◽  
Author(s):  
Zofia Sokołowicz ◽  
Józefa Krawczyk ◽  
Magdalena Dykiel
Keyword(s):  
Rhode Island ◽  
Egg Quality ◽  
Egg Yolk ◽  
Shape Index ◽  
Cholesterol Content ◽  
Housing System ◽  
Shell Weight ◽  
Alternative Housing ◽  
Egg Yolks ◽  
Yolk Cholesterol

The aim of the study was to determine the effect of type of alternative housing system and layer genotype on the quality of table eggs. The investigated eggs came from native Greenleg Partridge hens (Z-11) and Rhode Island Red hens (R-11) covered by the gene-pool protection program, as well as from Hy-line Brown commercial hens from litter barn (LS), free range (FR), and organic systems (OS). Eggs for the study were collected from the hens at 56 wk of age. Egg quality assessment was based on the following values egg traits: weight, shape index, yolk, albumen, and shell percentage in the whole egg; eggshell traits: color intensity, weight, thickness, density, breaking strength, physical features of egg content: albumen height, value of Haugh units, yolk color; presence of meat/ blood spots and cholesterol, higher fatty acids, vitamin A and E in egg yolks. In the rearing systems under study, eggs with the highest weight were laid by commercial hens, and the type of alternative rearing system had no significant effect on this trait. Shell colour intensity depends on the breed of hen, whereas the rearing system has no significant effect on this trait. Hen genotype and housing system had an effect on egg shell weight, thickness, and density. The housing system influences the content of MUFA, PUFA, PUFAn-6 and PUFAn-3 as well as PUFA 6/3 in egg yolks. No effect of the housing system on egg yolk cholesterol content was observed.

scholarly journals The Effect of Supplementation of Avocado Seed Flour (Persea americana Mill.) in Feed on Blood Lipids Profile and Egg Yolk Cholesterol of Japanese Quail (Corturnix-corturnix japonica)

2021 ◽  
pp. 10-17
Author(s):  
Yosua Sujud Apriyanto ◽  
Ning Iriyanti ◽  
Elly Tugiyanti
Keyword(s):  
Blood Lipids ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Egg Yolk ◽  
Persea Americana ◽  
Blood Cholesterol ◽  
Seed Flour ◽  
Randomized Design ◽  
Yolk Cholesterol ◽  
Avocado Seed

The purpose of this research is to determine the effect of avocado seed flour (ASF) supplementation in feed on blood lipids and quail egg yolk cholesterol. The materials were 100 female quails aged 4 weeks old with ration ingredients consist of corn, bran, paddy, soybean meal, fish flour, avocado seed flour, palm oil, CaCO3, premix, lysine, and methionine. The research conducted an experiment with 4 treatments of ASF supplementation levels incorporated into basal feed, namely 0% ASF (R0), 3% ASF (R1), 6% ASF (R2), and 9% ASF (R3). The observed variables included blood cholesterol, High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL), triglyceride, and egg yolk cholesterol. The data were subjected to the one-way Analysis of variance (ANOVA) in a completely randomized design, followed by HSD test when differences between treatments were observed. The result showed that ASF supplementation significantly reduced blood cholesterol levels and the egg yolk cholesterol, did not significantly affect LDL and triglyceride levels. The HDL levels and egg yolk cholesterol tend to decrease as the level of ASF increased. It is concluded that avocado seed flour (ASF) supplementation up to 9% is safe for quail feed supplementation.

scholarly journals Performance and hypo-cholesterolemic response of laying hens fed cassava root sievate - based diets.

2021 ◽  
Vol 32 (2) ◽  
pp. 215-223
Author(s):  
O.M.O. Idowu ◽  
A. Oduweso ◽  
E. Daisy
Keyword(s):  
Blood Plasma ◽  
Total Cholesterol ◽  
Egg Production ◽  
Dietary Treatment ◽  
Egg Yolk ◽  
Cholesterol Content ◽  
The Body ◽  
Cholesterol Concentration ◽  
Control Group ◽  
Yolk Cholesterol

A total of one hundred and fifty (150) Nera ser-linked layer strains (32 weeks of age) were used to investigate the performance response, blood plasma and egg yolk cholesterol status of hens fed cassava roul sievate (CRS) bused - diets. CRS was used to replace wheat offal at 0, 5, 10, 15 und 20% respectively, resulting in five dietary treatments. Each treatment had 3 replicates with each replicate group having five hirds. The experiment lasted for 10 weeks. No significant effect of diers was noticed in the body weights, weight gain and daily feed intake. Fced conversion per dozen eggs, hen-day egg production and total egg produced significantly declined as CRS levels increased from 0 to 20% in the diets. Diets with 20% CARS had the poorest performance. However, the blood plasma, egg yolk and egg cholesterol concentrations were reduced significantly (p< 0.05) with increased level of CRS Dietary treatment with 20 % CARS had the lowest (P< 0.05) cholesterol concentration. Inclusion of up to 20% CRS led to 35% reduction in plasma total cholesterol, while 18 and 17% reductions were obtained in the egg yolk total cholesterol and total cholesterol per egy respectively. Replacement of up to 10% (w/w) wheat offal by CRS is therefore recommended 10 effectively reduce cholesterol content of the plasma ,egg yolk and whole egg by 20%, 10% and 17 % respectively at performance level that are comparable with that of the control group. 

scholarly journals Low-density lipoprotein uptake and cholesterol accumulation by cultured renal cells.

1991 ◽  
Vol 2 (6) ◽  
pp. 1101-1107
Author(s):  
S Hashimoto ◽  
G T Nagami
Keyword(s):  
Cholesteryl Ester ◽  
Total Cholesterol ◽  
Apical Membrane ◽  
Mdck Cells ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Cholesterol Content ◽  
Low Density ◽  
Ok Cells ◽  
Ldl Uptake

The uptake of low-density lipoprotein (LDL) and the accumulation of cholesterol were assessed in opossum kidney (OK) and Madin-Darby canine kidney (MDCK) cells. OK and MDCK cells were grown to confluency on Millicell well inserts. The uptake of human LDL across the apical and basolateral surfaces of OK and MDCK cells was assessed by the degradation of internalized (125I)LDL to trichloroacetic acid-soluble products. LDL uptake via the apical surface of OK cells increased linearly with LDL concentration, indicating nonreceptor-mediated uptake. In contrast, LDL uptake via the basolateral surface of OK cells and both apical and basolateral surfaces of MDCK cells followed a saturable pattern. In addition, (125I)LDL bound to the apical membrane of MDCK cells, but not to the apical membrane of OK cells, was displaced by heparin and by excess of unlabeled LDL. Exposure to LDL (100 mg/mL) resulted in an increase in total cholesterol content of OK and MDCK cells (23 and 18%, respectively). Most of the increase in total cholesterol content with LDL exposure resulted from increased free cholesterol content in MDCK cells and esterified cholesterol in OK cells. The differences in cholesteryl ester formation were consistent with the slower rates of (14C) oleate incorporation into cholesteryl ester and lower cholesterol esterifying activity observed in MDCK cells compared with that in OK cells. These results demonstrate that LDL uptake can be receptor or nonreceptor mediated, depending upon the renal cell type and the surface exposed to LDL, and that LDL exposure leads to increased cholesterol content in OK and MDCK cells.

Hyperlipidaemia in cisplatin-induced nephrotic rats

1999 ◽  
Vol 18 (7) ◽  
pp. 454-459 ◽  
Author(s):  
A A Abdel-Gayoum ◽  
K B El-Jenjan ◽  
K A Ghwarsha
Keyword(s):  
Serum Creatinine ◽  
Total Cholesterol ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Total Bilirubin Level ◽  
Hdl Cholesterol ◽  
Cholesterol Content ◽  
Serum Total Cholesterol ◽  
Serum Total Bilirubin ◽  
Function Test

1 The serum and hepatic lipid concentrations were investigated in rats made nephrotic with a single intraperitoneal injection of cisplatin (6 mg kg-1 b.wt.). 2 The serum creatinine and urea concentrations were estimated as indices of nephrotoxicity, and the serum total bilirubin level as a liver function test. 3 The fasting serum total cholesterol, triglycerides (TG) and the cholesterol fractions associated with the various lipoproteins, as well as hepatic cholesterol and TG contents were also measured, following 5, 10 and 15 days from the cisplatin treatment. 4 The results revealed that on day 5 both serum creatinine and urea concentrations were significantly (P50.01) increased, indicating the peak of nephrotoxicity, with no injurious effects on the liver as indicated by the unaltered serum bilirubin concentration. 5 The nephrotoxicity was accompanied by significant elevations in serum total cholesterol and TG concentrations by 49 and 42%, respectively, with significant (P50.05) correlations between the serum cholesterol and TG concentrations versus the serum urea (r=0.68 and r=0.60, respectively). 6 Among the estimated lipoproteins, very low density lipoprotein (VLDL) cholesterol was severely increased to more than twofold with no severe changes in LDL or HDL-cholesterol fractions. On day 5 the liver also showed significant accumulation of TG with no change in the cholesterol content. 7 Animals killed 10 or 15 days post-cisplatin treatment had all the perturbed parameters returned to the normal levels. 8 The present results indicated that rats exposed to a single cisplatin injection exhibit acute reversible nephrosis on day 5 which was accompanied by dyslipidaemia and accumulated liver TG.

Risk predictors of arterial hypertension development in population of Mountain Shoriya of various ethnical origins

2019 ◽  
Vol 1 (3) ◽  
pp. 39-42
Author(s):  
T. A. Mulerova ◽  
M. Yu. Ogarkov ◽  
O. L. Barbarash
Keyword(s):  
Cardiovascular Diseases ◽  
Total Cholesterol ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density Lipoprotein Cholesterol ◽  
Type Settlement ◽  
The Family ◽  
Genotype C ◽  
Hypertension Development ◽  
Risk Predictors

1409 people (901 Shors, 508 non-indigenous people) from remote villages of Mountain Shoriya (Orton and Ust-Kabyrsa) and urban-type settlement Sheregesh took part in the survey. In Shors, the risk of developing hypertension was determined by elevated levels of total cholesterol and low density lipoprotein cholesterol, violation of carbohydrate metabolism, obesity, including its abdominal type, the family anamnesis of early cardiovascular diseases, and a carriage of prognostically unfavorable genotypes D/D and C/C of the corresponding genes ACE and AGTR 1 candidates; in the cohort of non-indigenous ethnos-elevated levels of total cholesterol and triglycerides, obesity, abdominal obesity, the family anamnesis of early cardiovascular diseases, a carrier of the minor genotype C/C of the AGTR 1 gene

scholarly journals Observational Study of Lipid Profile and C-Reactive Protein after a Seven-Day Fast

Nutrients ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 255
Author(s):  
Valeria Galetti ◽  
Marica Brnic ◽  
Benjamin Lotin ◽  
Mauro Frigeri
Keyword(s):  
Total Cholesterol ◽  
Medical Center ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Blood Parameters ◽  
Swiss Alps ◽  
Risk Level ◽  
Metabolic Risk ◽  
C Reactive Protein ◽  
Reactive Protein

Fasting is becoming an increasingly popular practice. Nevertheless, its clinical benefits and possible inconveniences remain limitedly evaluated. We observed the effects of a seven-day fast conducted in a non-medical center located in the Swiss Alps. Clinical parameters were measured on the first and last day of fasting (D1 and D7), and two months later (D60). Among the 40 participants, blood analyses were done on 25 persons with an increased metabolic risk, with the primary goal of assessing the lasting effect on low-density lipoprotein (LDL) cholesterol. By comparing D60 with D1, high-density lipoprotein cholesterol (HDL) (+0.15 mmol/L) and insulin-like growth factor-1 (IGF-1) (+2.05 mmol/L) increased (both p < 0.009), all other blood parameters (LDL, glucose, total cholesterol, triglycerides, C-reactive protein (CRP)) did not change; weight (−0.97 kg) and hearth rate (−7.31 min−1) decreased (both p < 0.006). By comparing D7 with D1, total cholesterol (+0.44 mmol/L), triglycerides (+0.37 mmol/L) and CRP (+3.37 mg/L) increased (all p < 0.02). The lack of LDL variation at D60 may be due to the low metabolic risk level of the participants. The increase of total cholesterol, triglycerides and CRP at D7 warrants studies to understand whether such fluctuations represent a stress reaction to the fasting state, which may vary in different fasting types.

Characterization of glycopeptides derived from the low-density lipoprotein of hen's egg yolk

1968 ◽  
Vol 46 (8) ◽  
pp. 983-988 ◽  
Author(s):  
J. Z. Augustyniak ◽  
W. G. Martin
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Egg Yolk ◽  
Low Density ◽  
Amide Nitrogen ◽  
Acetylneuraminic Acid ◽  
Hen’S Egg ◽  
Terminal Amino ◽  
Protein Moiety

Two glycopeptides (A and B) were isolated from pronase-digested vitellenin, the protein moiety of the low-density lipoprotein of hen's egg yolk. Aspartic acid was the only N-terminal amino acid of both glycopeptides but only A contained N-acetylneuraminic acid. A contained 55% hexose (mannose), 14% hexosamine, 12% N-acetylneuraminic acid, 0.71% amide nitrogen, and its molecular weight was 2.3 × 103. The corresponding values for B were 64, 17, 0.0, 0.75, and 2.0 × 103. Chemical analyses showed that B (and probably A) occurs in vitellenin with the heteropolysaccharide group bound N-glycosidically via the β-amide group of an asparaginyl residue. The indicated structure is R∙(NH)Asp∙Thr∙Ser∙(Ala, Gly, Val)∙Ile, where R, the heteropolysaccharide group, contains 2 hexosamine and 8 hexose residues.

scholarly journals Glycosylation inhibition reduces cholesterol accumulation in NPC1 protein-deficient cells

2015 ◽  
Vol 112 (48) ◽  
pp. 14876-14881 ◽  
Author(s):  
Jian Li ◽  
Maika S. Deffieu ◽  
Peter L. Lee ◽  
Piyali Saha ◽  
Suzanne R. Pfeffer
Keyword(s):  
Low Density Lipoprotein ◽  
Human Fibroblasts ◽  
Chinese Hamster ◽  
Density Lipoprotein ◽  
Cholesterol Content ◽  
Protein Glycosylation ◽  
Sugar Addition ◽  
Protein Functions ◽  
D Cells ◽  
Niemann Pick

Lysosomes are lined with a glycocalyx that protects the limiting membrane from the action of degradative enzymes. We tested the hypothesis that Niemann-Pick type C 1 (NPC1) protein aids the transfer of low density lipoprotein-derived cholesterol across this glycocalyx. A prediction of this model is that cells will be less dependent upon NPC1 if their glycocalyx is decreased in density. Lysosome cholesterol content was significantly lower after treatment of NPC1-deficient human fibroblasts with benzyl-2-acetamido-2-deoxy-α-D-galactopyranoside, an inhibitor of O-linked glycosylation. Direct biochemical measurement of cholesterol showed that lysosomes purified from NPC1-deficient fibroblasts contained at least 30% less cholesterol when O-linked glycosylation was blocked. As an independent means to modify protein glycosylation, we used Chinese hamster ovary ldl-D cells defective in UDP-Gal/UDP-GalNAc 4-epimerase in which N- and O-linked glycosylation can be controlled. CRISPR generated, NPC1-deficient ldl-D cells supplemented with galactose accumulated more cholesterol than those in which sugar addition was blocked. In the absence of galactose supplementation, NPC1-deficient ldl-D cells also transported more cholesterol from lysosomes to the endoplasmic reticulum, as monitored by an increase in cholesteryl [14C]-oleate levels. These experiments support a model in which NPC1 protein functions to transfer cholesterol past a lysosomal glycocalyx.

scholarly journals High serum cholesterol levels in persons with 'high-normal' TSH levels: should one extend the definition of subclinical hypothyroidism?

1998 ◽  
pp. 141-145 ◽  
Author(s):  
G Michalopoulou ◽  
M Alevizaki ◽  
G Piperingos ◽  
D Mitsibounas ◽  
E Mantzos ◽  
...  
Keyword(s):  
Total Cholesterol ◽  
Serum Cholesterol ◽  
Subclinical Hypothyroidism ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Serum ◽  
High Serum Cholesterol ◽  
Cholesterol Levels ◽  
Group D ◽  
Tsh Levels

OBJECTIVE: The association between established hypothyroidism and high cholesterol levels is well known. The aim of the present study was to investigate the effect of thyroxine (T4) administration on cholesterol levels in hypercholesterolemic subjects with TSH levels within the normal range ('high-normal' TSH compared with 'low-normal' TSH). DESIGN AND METHODS: We determined TSH levels in 110 consecutive patients referred for hypercholesterolemia (serum cholesterol >7.5 mmol/l). Those with 'high-normal' TSH (2.0-4.0 microU/ml) as well as those with 'low-normal' TSH (0.40-1.99 microU/ml) were randomly assigned to receive either 25 or 50 microg T4 daily for two months. Thus, groups A and B (low-normal TSH) received 25 and 50 microg T4 respectively and groups C and D (high-normal TSH) received 25 and 50 microg T4 respectively. Serum T4, tri-iodothyronine (T3), TSH, free thyroxine index, resin T3 uptake and thyroid autoantibodies (ThAab) as well as total cholesterol, high and low density lipoprotein cholesterol (HDL, LDL), and triglycerides were determined before and at the end of the two-month treatment period. RESULTS: TSH levels were reduced in all groups. The most striking effect was observed in group D (TSH levels before: 2.77+/-0.55, after: 1.41+/-0.85 microU/ml, P < 0.01). Subjects in groups C and D had a higher probability of having positive ThAabs. A significant reduction in total cholesterol (P < 0.01) and LDL (P < 0.01) was observed after treatment only in group D. In those subjects in group D who were ThAab negative, there was no significant effect of thyroxine on cholesterol levels. CONCLUSIONS: Subjects with high-normal TSH levels combined with ThAabs may, in fact, have subclinical hypothyroidism presenting with elevated cholesterol levels. It is possible that these patients might benefit from thyroxine administration.

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