scholarly journals The Effect of Supplementation of Avocado Seed Flour (Persea americana Mill.) in Feed on Blood Lipids Profile and Egg Yolk Cholesterol of Japanese Quail (Corturnix-corturnix japonica)

2021 ◽  
pp. 10-17
Author(s):  
Yosua Sujud Apriyanto ◽  
Ning Iriyanti ◽  
Elly Tugiyanti

The purpose of this research is to determine the effect of avocado seed flour (ASF) supplementation in feed on blood lipids and quail egg yolk cholesterol. The materials were 100 female quails aged 4 weeks old with ration ingredients consist of corn, bran, paddy, soybean meal, fish flour, avocado seed flour, palm oil, CaCO3, premix, lysine, and methionine. The research conducted an experiment with 4 treatments of ASF supplementation levels incorporated into basal feed, namely 0% ASF (R0), 3% ASF (R1), 6% ASF (R2), and 9% ASF (R3). The observed variables included blood cholesterol, High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL), triglyceride, and egg yolk cholesterol. The data were subjected to the one-way Analysis of variance (ANOVA) in a completely randomized design, followed by HSD test when differences between treatments were observed. The result showed that ASF supplementation significantly reduced blood cholesterol levels and the egg yolk cholesterol, did not significantly affect LDL and triglyceride levels. The HDL levels and egg yolk cholesterol tend to decrease as the level of ASF increased. It is concluded that avocado seed flour (ASF) supplementation up to 9% is safe for quail feed supplementation.

2018 ◽  
Vol 43 (4) ◽  
pp. 396
Author(s):  
L.T. Wulandari ◽  
N. Suthama ◽  
B. Sukamto

The study was aimed to evaluate the effectiveness of feeding dietary microparticles protein added with Lactobacillus sp. on blood parameters and broiler productivity. A total of 192 birds of 21 days old broiler with initial body weight of 481 ± 67 g were divided into 8 treatments and 4 replications (6 birds each) of a completely randomized design (CRD). Dietary treatments were T0: 21% intact protein ration, T1: 18% intact protein ration, T2: 21% microparticle protein ration, T3: 18% microparticle protein ration, T4: T0 + 1.2 mL Lactobacillus sp, T5: T1 + 1.2 mL Lactobacillus sp., T6: T2 + 1.2 mL Lactobacillus sp., and T7: T3 + 1.2 mL Lactobacillus sp. Parameters observed were total lactic acid bacteria and Coliform, pH, blood cholesterol, low density lipoprotein (LDL), high density lipoprotein (HDL), meat cholesterol, meat weight, feed consumption, and body weight gain (BWG). Data were analyzed using analysis of variance and followed by Duncan test (P<0.05). The results showed that T7 treatment significantly (P<0.05) decreased blood cholesterol, LDL, and meat cholesterol, but increased HDL, meat weight, and BWG. Blood cholesterol, LDL, and meat cholesterol were indicated significantly (P<0.05) lowest values due to T7 treatment. In conclusion, feeding 18% microparticle protein with addition of 1.2 mL Lactobacillus sp. can improves blood status and increases broiler productivity.


2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Nai Nurazizah ◽  
Nai Nurazizah

The research was conducted to know the effect of noni fruit extract supplemented by Cu and Zn on blood cholesterol and  egg yolk cholesterol, creatinin, and blood urea nitrogen of sentul chicken. The research was done from August until October 2019. Samples test was held in Physiology and Biochemistry Laboratory, Animal Husbandry Faculty, Padjadjaran University, Sumedang. The methods used experimental with a Completely Randomized Design (CRD) and the effect of treatment using Analysis of  Varience (ANOVA) followed by Orthogonal Polynomial. The treatment consisted of five kind with five repetitions, those are to =  without adding noni fruit extract and  Cu, Zn , t1= Ration + 60 mg/kg extract noni fruit suplemented Cu 0,06 mg and Zn 1,5 mg, t2= Ration+ 180 mg/kg extract fruit noni suplemented Cu 0,18 mg and Zn 3,0 m, t3= Ration + 240 mg/kg extract fruit noni suplemented Cu 0,24 mg and Zn 4,5 mg, t4= Ration + 360 mg/kg extract fruit noni suplemented Cu 0,36 and Zn 6,0 mg. The result showed that the effect of giving 360 mg/kg noni extract suplemented Cu 0,36 and Zn 6,0 mg was significant effect  decreasing blood cholesterol levet but non significant  decreasing egg yolk cholesterol, creatinin, and blood urea nitrogen levels of Sentul Chicken. Keywords:  noni fruit extract, cholesterol, urea, creatinin, blood, egg yolk              cholesterol, sentul chicken, mineral Cu and ZnThe research was conducted to know the effect of noni fruit extract supplemented by Cu and Zn on blood cholesterol and  egg yolk cholesterol, creatinin, and blood urea nitrogen of sentul chicken. The research was done from August until October 2019. Samples test was held in Physiology and Biochemistry Laboratory, Animal Husbandry Faculty, Padjadjaran University, Sumedang. The methods used experimental with a Completely Randomized Design (CRD) and the effect of treatment using Analysis of  Varience (ANOVA) followed by Orthogonal Polynomial. The treatment consisted of five kind with five repetitions, those are to =  without adding noni fruit extract and  Cu, Zn , t1= Ration + 60 mg/kg extract noni fruit suplemented Cu 0,06 mg and Zn 1,5 mg, t2= Ration+ 180 mg/kg extract fruit noni suplemented Cu 0,18 mg and Zn 3,0 m, t3= Ration + 240 mg/kg extract fruit noni suplemented Cu 0,24 mg and Zn 4,5 mg, t4= Ration + 360 mg/kg extract fruit noni suplemented Cu 0,36 and Zn 6,0 mg. The result showed that the effect of giving 360 mg/kg noni extract suplemented Cu 0,36 and Zn 6,0 mg was significant effect  decreasing blood cholesterol levet but non significant  decreasing egg yolk cholesterol, creatinin, and blood urea nitrogen levels of Sentul Chicken. Keywords:  noni fruit extract, cholesterol, urea, creatinin, blood, egg yolk              cholesterol, sentul chicken, mineral Cu and Zn


Author(s):  
Sumardi Sumardi ◽  
Sutyarso Sutyarso ◽  
Gregorius Nugroho Susanto ◽  
Tintin Kurtini ◽  
Madi Hartono ◽  
...  

The aim of this research was to find out the effect of probiotic on blood cholesterol in laying hens. Materials used were laying hens (Isa Brown strain) 43 weeks old and ration using probiotics. This research was conducted by using completely randomized design (CRD). The advanced research was done with testing the local probiotic with various concentration treatment, they are P0 (ration without probiotic), P1 (ration + 1% local probiotic), P2 (ration + 2% local probiotic), and P3 (ration + 3% local probiotic) with 5 time repetitions and in every repetitions consists of 2 chicken. Variables that were observed in the advance research were laying hens’s blood cholesterol that consists of total cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), triglycerides, and total leucocyte. The level of total cholesterol on P0, P1, P2, and P3 were 129.00±38.26, 116.00± 26.53, 121.00±33.72, and 137.00±52.53 mg/dL (P>0.05) respectively; the HDL level were 50.60±16.47, 35.80±11.28, 59.40±19.74; 56.80±30.56 mg/dL (P>0.05); the LDL level were 100.20±49.53, 39.20±17.14, 104.60±51.23, and 96.80±66.22 mg/dL (P>0.05), respectively; the trigycerides level were 862.00±355.5, 533.00±146.1, 829.00±242.6, and 810.00±304 mg/dL (P>0.05), respectively; and leucocyte count were 22.00±1.72, 21.00±3.79, 31.00±3.99, and 34.00±3.55 x103 mm3 (P<0.01), respectively. In conclusion, the administrtion of local probiotic do not effect the blood cholesterol of layer chicken.


2016 ◽  
Vol 1 (2) ◽  
pp. 1-5
Author(s):  
Nurul Fajrih

The feeding inulin of dahlia tubers as a prebiotic related to the quality of the resulting product is a product low cholesterol. The research was aimed to examine the role of inulin as a prebiotic derived from dahlia flower tuber in the form of powder and extract on blood cholesterol, low density lipoprotein (LDL) and high density lipoprotein (HDL) of crossbred local chicken. The research was assigned in a completely randomized design with 7 treatments and 4 replications (10 birds each), treatments applied were T0: basal ration, T1: ration + 0.4% powder of dahlia tuber, T2: ration + 0.8% powder of dahlia tuber, T3: ration + 1.2% powder of dahlia tuber, T4: ration + 0.39% extract of dahlia tuber, T5: ration + 0.78% extract of dahlia tuber, T6: ration + 1.17% extract of dahlia tuber. The birds were reared for conditioning from day 1 until 3 week, and dietary treatment was given thereafter until 11 week of age. Parameters observed were blood cholesterol, LDL and HDL. The data were statistically analyzed according to ANOVA and continued to Duncan test at the level of 5% probability. The results showed that feeding inulin in the form of powder or extract significantly (P<0,05) decreased LDL and HDL, but not on blood cholesterol. In conclusion, feeding inulin in the form of powder in 1.2% (T3) and extract in 1.17% (T6), able to reduce levels of LDL and HDL but not yet capable of lowering blood cholesterol of crossbred local chicken.


2021 ◽  
Vol 4 (2) ◽  
pp. 14-23
Author(s):  
Lady Violita ◽  
Rasita Purba ◽  
Marini Damanik ◽  
Juliarti ◽  
Esi Emilia

Cookies are one of the food products that are popular with the public. The raw material for making cookies is wheat flour, which is still imported from abroad and year by year the number of imports from abroad continues to increase. Utilization of avocado seed flour will reduce the use of wheat flour and can also reduce waste from avocados. The main factor in fulfilling the quality of a product can be assessed based on the appearance of the product, the taste of the product, and the nutritional value of a product. This study aims to analysed the level of preference (taste, aroma, texture, and colour) of cookies through organoleptic tests. The design of this study used an experimental study using the RAL (Completely Randomized Design) method with two replications. Avocado seed flour substitution formulation in 4 treatments, namely control (P1), 30% (P2), 50% (P3) and 70% (P4). The research location is at the State University of Medan. The subjects (untrained panellists) of this study were 25 people. The data from the organoleptic test were analysed descriptively using the percentage of respondents' acceptance from each treatment level and to determine the effect of avocado seed flour substitution on the treatment, Kruskal Wallis statistical analysis was used. The results of this study indicate that the best formula for cookies with 50 percent substitution of avocado seed flour obtained an average value of 1.2 for taste, 0.3 for colour, 0.6 for texture, and for aroma 0.3 with a total value of 2, 4. Based on the results of the Kruskal Wallis test, it is known that there is a significant difference in perception seen from the level of preference for colour, aroma, taste, and texture of avocado seed flour cookies (p = 0.000).


2020 ◽  
Vol 9 (5) ◽  
pp. 1596 ◽  
Author(s):  
Friederike Zimmermann ◽  
Johann Roessler ◽  
David Schmidt ◽  
Andrzej Jasina ◽  
Paul Schumann ◽  
...  

Background and Aims: The mechanisms of interindividual variation of lipid regulation by statins, such as the low-density lipoprotein cholesterol (LDL) lowering effects, are not fully understood yet. Here, we used a gut microbiota depleted mouse model to investigate the relation between the gut microbiota and the regulatory property of atorvastatin on blood lipids. Methods: Mice (C57BL/6) with intact gut microbiota or antibiotic induced abiotic mice (ABS) were put on standard chow diet (SCD) or high fat diet (HFD) for six weeks. Atorvastatin (10 mg/kg body weight/day) or a control vehicle were applied per gavage for the last four weeks of dietary treatment. Blood lipids including total cholesterol, very low-density lipoprotein, low-density lipoprotein, high-density lipoprotein and sphingolipids were measured to probe microbiota-dependent effects of atorvastatin. The expression of genes involved in hepatic and intestinal cholesterol metabolism was analyzed with qRT-PCR. The alteration of the microbiota profile was examined using 16S rRNA qPCR in mice with intact gut microbiota. Results: HFD feeding significantly increased total blood cholesterol and LDL levels, as compared to SCD in both mice with intact and depleted gut microbiota. The cholesterol lowering effect of atorvastatin was significantly attenuated in mice with depleted gut microbiota. Moreover, we observed a global shift in the abundance of several sphingolipids upon atorvastatin treatment which was absent in gut microbiota depleted mice. The regulatory effect of atorvastatin on the expression of distinct hepatic and intestinal cholesterol-regulating genes, including Ldlr, Srebp2 and Npc1l1 was altered upon depletion of gut microbiota. In response to HFD feeding, the relative abundance of the bacterial phyla Bacteroidetes decreased, while the abundance of Firmicutes increased. The altered ratio between Firmicutes to Bacteroidetes was partly reversed in HFD fed mice treated with atorvastatin. Conclusions: Our findings support a regulatory impact of atorvastatin on the gut microbial profile and, in turn, demonstrate a crucial role of the gut microbiome for atorvastatin-related effects on blood lipids. These results provide novel insights into potential microbiota-dependent mechanisms of lipid regulation by statins, which may account for variable response to statin treatment.


Author(s):  
I Nyoman Sukartha Jaya ◽  
Ni Ketut Dewi Haryani ◽  
Budi Indarsih

The objective of this study to determine the effect of feeding fresh garlic to ducks on decrease the cholesterol level of duck eggs. This study composed of two treatment groups. The duck in the first group (control) was fed local feed without spplemente d with fresh garlic, while in the second group was fed with local feed and supplemented with 50 grams of fresh garlic/duck/day. Egg yolk cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and triglycerides (TG) analysis were carried out at the end of the study using the ether extract method and then with the Cholesterol Oksidase Para Amino Penazone (CHOD-PAP) or enzymatic color test. Data was analysed using one way Analysis of Variance (ANOVA) the results showed that fresh garlic reduced total cholesterol, LDL fraction, and triglyceride, but increased the HDL fraction of duck egg yolk. The total cholesterol, LDL fraction, and triglyceride of duck egg yolks dropped by around 15.98 mg per dL (8.06%), 10.40 mg per dL (12.97%), 64.92 mg per dL (12.71%), respectively, while the HDL fraction increased by 11.40 mg per dL (19.32%) The results of this study indicated that fresh garlic can be used to produce specific products such as low cholesterol and low fat duck eggs.


1968 ◽  
Vol 46 (8) ◽  
pp. 983-988 ◽  
Author(s):  
J. Z. Augustyniak ◽  
W. G. Martin

Two glycopeptides (A and B) were isolated from pronase-digested vitellenin, the protein moiety of the low-density lipoprotein of hen's egg yolk. Aspartic acid was the only N-terminal amino acid of both glycopeptides but only A contained N-acetylneuraminic acid. A contained 55% hexose (mannose), 14% hexosamine, 12% N-acetylneuraminic acid, 0.71% amide nitrogen, and its molecular weight was 2.3 × 103. The corresponding values for B were 64, 17, 0.0, 0.75, and 2.0 × 103. Chemical analyses showed that B (and probably A) occurs in vitellenin with the heteropolysaccharide group bound N-glycosidically via the β-amide group of an asparaginyl residue. The indicated structure is R∙(NH)Asp∙Thr∙Ser∙(Ala, Gly, Val)∙Ile, where R, the heteropolysaccharide group, contains 2 hexosamine and 8 hexose residues.


2015 ◽  
Vol 6 (1) ◽  
pp. 29-39 ◽  
Author(s):  
A.T. Bjerg ◽  
M. Kristensen ◽  
C. Ritz ◽  
K.D. Stark ◽  
J.J. Holst ◽  
...  

The microbiota has been shown to have the potential to affect appetite and blood lipids positively in animal studies. We investigated if four weeks supplementation with Lactobacillus paracasei subsp. paracasei L. casei W8® (L. casei W8) had an effect on subjective appetite sensation, ad libitum energy intake, glucagon-like peptide 1 (GLP-1), glucose and insulin response in humans. Secondarily, we explored potential effects on blood lipids, fatty acids and stearoyl-CoA desaturase-1 (SCD1) activity in humans as well as SCD1 expression in piglets given L. casei W8 for two weeks. 64 healthy participants completed the double-blinded, randomised, controlled, parallel four weeks study with supplementation of L. casei W8 (1010 cfu) or placebo capsules. A meal test was conducted before and after the intervention, where subjective appetite, ad libitum energy intake, GLP-1, glucose and insulin response were measured. Additionally fasting blood lipids and fatty acids concentrations were measured. Sixteen piglets were randomised into two groups: L. casei W8 (1010 cfu/day) as top dressing on morning fed or no treatment. After two weeks piglets were sacrificed and tissue from ileum, jejunum and skeletal muscle were sampled for mRNA analyses of SCD1 expression. Compared to placebo, L. casei W8 did not affect appetite, ad libitum energy intake, GLP-1, glucose and insulin response and total, high-density or low-density lipoprotein cholesterol levels after four weeks intervention. Triacylglycerol decreased in the L. casei W8 group compared to placebo at week 4 (P=0.03). The C16:1n-7/C16:0 ratio, reflecting SCD1 activity, tended to decrease when having L. casei W8 (P=0.06) compared to placebo. Muscle SCD1 expression decreased in piglets supplemented with L. casei W8 compared to control. In conclusion, supplementation with L. casei W8 did not affect appetite parameters, glucose or insulin responses; but appear to be able to lower triacylglycerol levels, possibly by reducing its production.


BMJ Open ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. e026148
Author(s):  
Megali Mansour ◽  
Hani Tamim ◽  
Lara Nasreddine ◽  
Christelle El Khoury ◽  
Nahla Hwalla ◽  
...  

ObjectiveTo examine associations of behavioural risk factors, namely cigarette smoking, physical activity, dietary intakes and alcohol consumption, with blood lipids profile.Design and participantsData drawn from a cross-sectional study involving participants aged 18 years and over (n=363) from the nationwide WHO STEPwise Nutrition and Non-communicable Disease Risk Factor survey in Lebanon.MeasuresDemographic characteristics, behaviours and medical history were obtained from participants by questionnaire. Dietary assessment was performed using a 61-item Culture-Specific Food Frequency Questionnaire that measured food intake over the past year. Lipid levels were measured by the analysis of fasting blood samples (serum total cholesterol (TC), triglycerides (TG), very low-density lipoprotein (VLDL), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C)).ResultsCurrent cigarette smoking, alcohol consumption and low physical activity were prevalent among 33.3%, 39.7% and 41.6% of the sample, respectively. The contributions of fat and saturated fat to daily energy intake were high, estimated at 36.5% and 11.4%, respectively. Abnormal levels of TC, TG, VLDL, LDL-C and HDL-C were observed for 55.4%, 31.4%, 29.2%, 47.5% and 21.8% of participants, respectively. Adjusting for potential confounders, cigarette smoking was positively associated with higher odds of TG and VLDL (OR=4.27; 95% CI 1.69 to 10.77; and 3.26; 95% CI 1.33 to 8.03, respectively) with a significant dose–response relationship (p value for trend=0.010 and 0.030, respectively). Alcohol drinking and high saturated fat intake (≥10% energy intake) were associated with higher odds of LDL-C (OR=1.68; 95% CI 1.01 to 2.82 and OR= 1.73; 95% CI 1.02 to 2.93). Physical activity did not associate significantly with any blood lipid parameter.ConclusionThe demonstrated positive associations between smoking, alcohol drinking and high saturated fat intake with adverse lipoprotein levels lay further evidence for clinical practitioners, public health professionals and dietitians in the development of preventive strategies among subjects with a high risk of cardiovascular diseases in Lebanon and other neighbouring countries with similar epidemiological profile.


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