scholarly journals The Initial Antibacterial Activity Tests of Pliek U Oil and Pliek U: an Acehnese Traditional Food

2008 ◽  
Vol 2 (2) ◽  
Author(s):  
Nurliana Nurliana ◽  
Mirnawati Sudarwanto ◽  
Lisdar I. Sudirman ◽  
A. W. Sanjaya
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Diffusion Method ◽  
Traditional Food ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Agar Disc ◽  
Inhibition Zones ◽  
Initial Research

This initial research was intended to detect antibacterial activity of pliek u oil and pliek u. Pliek u oil consist of minyeuk simplah (MS) and minyeuk brok (MB), Pliek u consist of two kinds of solid waste namely wet pliek u (Ap1) and dry pliek u (Ap2). Pliek u was methanol extracted at concentration 10% (w/v). Pliek u oil and methanol extract of pliek u were evaluated for their antibacterial activity, against Bacillus subtilis and four strains of Enterophatogenic Escherichia coli (EPEC) employing agar disc diffusion method. No antibacterial activity was shown by MS. The MB exhibited a little effect 1-2 mm against bacterial tests. The results demonstrated that the higher antibacterial activity was shown by dry pliek u compare with wet pliek u with the inhibition zones from 6.67-10.33 mm and 6.00-7.33 mm respectively.

scholarly journals EVALUATION OF ANTIBACTERIAL ACTIVITY OF A CYANOBACTERIUM SCYTONEMA SCHMIDTII GOM

2021 ◽  
pp. 77-79
Author(s):  
Janardhan Namdeo Nehul
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Axenic Culture ◽  
Soil Samples ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Air Blower

Scytonema schmidtii,a cyanobacterium was isolated from the collected soil samples from different locations of Ahmednagar district of Maharashtra state (India). Identification was carried out using morphological variation and taxonomical approaches according to Desikachary (1959) and Prescott (1962) .The axenic culture of Scytonema schmidtii was obtained by using the method recommended by Bolch and Blackburn (1996). The isolated Scytonema schmidtii was grown autotropically in BG-11 medium as described by Rippka et al.,(1979) and incubated at 30±2°c.After 25 days, biomass was harvested by filtration through double layered muslin cloth and dried using air blower. The biomass of this Scytonema schmidtii species was used for the assessment of antibacterial activity against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus flavus and Proteus mirabilis. The antibacterial activity was studied by disc diffusion method (Anonymous,1996).Methanol extract of Scytonema schmidtii showed the activity against all the tested bacterial strains.Maximum zone of inhibition (23±1.9 mm) was recorded with methanol extract of Scytonema schmidtii.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK METANOL Acalypha hispida TERHADAP BAKTERI Shigella flexneri DAN Bacillus cereus IHB B 379

2020 ◽  
Vol 10 (2) ◽  
Author(s):  
Elvi Rusmiyanto P. Wardoyo ◽  
Devinda Ekarizky Diputri ◽  
Rikhsan Kurniatuhadi
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Methanol Extract ◽  
Shigella Flexneri ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Extract Concentration ◽  
Activity Test ◽  
Antibacterial Potential

AbstractAcalpha hispida flower commonly used as traditional medicine. The antibacterial potential of the A. hispida flower can be used to treat diarrhea and cancer. The purpose of this study was to determine the antibacterial activity and effective concentration of methanol extract of A. hispida flower against to S. flexneri and B. cereus. Sample used was flower of A. hispida from Kota Pontianak. The solvent used is metahnol. Antibacterial activity test using the disc diffusion method (Kirby-Bauer) with parameters based on grading levels  extract concentration in S. flexneri was 1.2;  1.3;  1.4;  1.5;  and 1.6 g/ml and B. cereus for 0.75;  0.80;  0.85;  0.90;  and 0.95 g/mL, 10% DMSO and ciprofloaxin as a comparison. the results of the research showed that the concentration of methanol extract of A. hispida flower which can effectively inhibit the growth of S. flexneri and B. cereus bacteria at 1.2 g / mL and 0.75 g / mL. The methanol extract of A. hispida flower contains compounds of alkaloids, flavonoids, tannins, phenols, steroids and terpenoids.Keywords : Acalypha hispida, Antibacterial, Shigella flexneri, Bacillus cereus AbstrakAcalypha hispida merupakan tanaman obat trardisional yang sudah dimanfaatkan masyarakat secara turun temurun untuk mengobati diare dan kanker. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri dan konsentrasi efektif ekstrak metanol bunga A. hispida terhadap bakteri Shigella flexneri dan Bacillus cereus. Sampel penelitian yang digunakan adalah bunga A. hispida yang diperoleh dari Kota Pontianak. Pelarut yang digunakan adalah methanol. Uji aktivitas antibakteri menggunakan metode difusi cakram (Kirby-Bauer) dengan perlakuan 5 taraf konsentrasi ekstrak pada bakteri S. flexneri sebesar 1,2; 1,3; 1,4; 1,5; dan 1,6 g/ml serta pada bakteri B. cereus IHB B 379 sebesar 0,75; 0,80; 0,85; 0,90; dan 0,95 g/mL, DMSO 10% serta sebagai pembanding digunakan ciprofloaxin. Hasil penelitian menunjukkan bahwa konsentrasi ekstrak methanol bunga A. hispida yang secara efektif dapat menghambat pertumbuhan bakteri S. flexneri dan B. cereus sebesar 1,2 g/mL dan 0,75 g/mL. Ekstrak methanol bunga A. hispida mengandung senyawa golongan alkaloid, flavonoid, tannin, fenol, steroid dan terpenoid.Kata kunci: Acalypha hispida, antibakteri, Shigella flexneri, Bacillus cereus

scholarly journals Antibacterial Activity of Eight Medicinal Plants against Multidrug Resistant Escherichia coli and Salmonella spp. isolated from Broiler Meat

2020 ◽  
Vol 3 (4) ◽  
pp. 28-48
Author(s):  
Md. Rayhan Ali ◽  
Md. Omar Faruque ◽  
Md. Tarek Molla ◽  
Roksana Khanam ◽  
Shahin Mahmud ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Salmonella Spp ◽  
Broiler Meat ◽  
Standard Disc

The excess use of antimicrobial agents in the poultry industry is a significant reason for the gradual spread and increasing level of multidrug resistance bacteria. This article is based on a study in which the antibacterial activity of aqueous, methanolic, ethanolic and acetonic extracts of eight medicinal plants were evaluated by standard disc diffusion method against multidrug resistant Escherichia coli and Salmonella spp. isolated from broiler meat. The multidrug resistance was checked by commercially available antibiotics using standard disc diffusion method. The results have indicated that the ethanolic extract of Boerhaavia diffusa showed maximum zone of inhibition against Escherichia coli, while Asparagus racemosus showed maximum zone of inhibition against Salmonella spp. Other experimental plant extracts had showed moderate activity against these multidrug-resistant bacteria, which can also be considered as potential source of active beneficial phytochemicals. Proper management and application of these plant extracts may be a wonderful alternative of commercially available antibiotic to minimize the risk.

scholarly journals Aktivitas Antibakteri Ekstrak Rumput Laut Eucheuma spinosum Segar dari Perairan Pulau Nain Sulawesi Utara

2021 ◽  
Vol 21 (1) ◽  
pp. 91
Author(s):  
Lena Jeane Damongilala ◽  
Fitje Losung ◽  
Verly Dotulong
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Ethyl Acetate Fraction ◽  
Disc Diffusion ◽  
Butanol Fraction ◽  
Inhibition Zones

Penelitian terhadap ekstrak metanol, fraksi etil asetat, dan fraksi butanol dari rumput laut Eucheuma spinosum menggunakan metode Disc Diffusion Test dilakukan untuk mengetahui peranannya sebagai antibakteri Escherichia coli ATCC 25922, dan Staphylococcus aureus ATCC 6538. Sebagai kontrol digunakan ekstrak (negatif) pelarut metanol, dan Amoxicilin. Konsentrasi sampel uji ditetapkan 10.000 ppm. Hasil pengujian menunjukkan adanya  aktivitas antibakteri yang ditandai dengan terbentuknya zona hambat terhadap bakteri Escherichia coli ATCC 25922.  Besarnya zona hambat secara berurutan untuk ekstrak metanol, fraksi  butanol, dan fraksi etil asetat, ialah : 6,98 mm, 7,85 mm, dan 7,88 mm.  Nilai zona hambat tersebut, relatif sama untuk setiap sampel uji. Sampel uji juga menunjukkan terdapat aktivitas antibakteri, ditandai dengan terbentuknya zona hambat terhadap bakteri Staphylococcus aureus ATCC 6538.  Besarnya zona hambat secara berurutan untuk ekstrak metanol, fraksi  butanol, fraksi etil asetat,  dan kontrol negatif pelarut metanol, ialah : 7,42 mm, 14.40 mm, 7,90 mm, dan 6,75 mm.  Zona hambat fraksi butanol lebih tinggi dibanding yang lain, ini menunjukkan bahwa aktivitas antibakteri Staphylococcus aureus pada fraksi butanol lebih tinggi dibanding fraksi lainnya.Kata kunci:  Antibakteri;  Eucheuma spinosum;  Escherichia coli; Staphylococcus aureus Antibacteria Activities of Extract Sea Algae  Eucheuma spinosum Fresh from Nain Island Waters North Sulawesi  ABSTRACTA research on antibacterial activity of methanol extract, ethyl acetate, and butanol fraction from Eucheuma spinosum seaweed using the disc diffusion method to know the role as  antibacterial activity was tested against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 6538. Methanol was used as negative control while amoxicillin was used as positive control. The concentration of the test sample was set at 10,000 ppm. The antibacterial activity was indicated by the formation of an inhibition zone against the tested microorganisms. The inhibition zones against E.coli for methanol extract, butanol fraction, and ethyl acetate fraction were 6.98 mm, 7.85 mm, and 7.88 mm, respectively.  The value of the zone of inhibition is relatively the same for each sample.  In addition, the inhibition zones of methanol extract, butanol and ethyl acetate fraction against Staphylococcus aureus were 7.42 mm, 14.40 mm, 7.90 mm, and 6.75 mm, respectively. The inhibition zone of the butanol fraction is higher than the others, this indicated that the antibacterial activity for butanol fraction is higher than other fractions. Keywords: Antibacterial activity; Eucheuma spinosum; Escherichia coli; Staphylococcus aureus

scholarly journals Evaluation of the antibacterial activity of bergamot essential oils on different Listeria monocytogenes strains

2016 ◽  
Vol 5 (4) ◽  
Author(s):  
Stefania M. Marotta ◽  
Filippo Giarratana ◽  
Alessio Parco ◽  
Domenico Neri ◽  
Graziella Ziino ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Listeria Monocytogenes ◽  
Essential Oils ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Strong Activity ◽  
Agar Disc ◽  
Inhibition Experiment

Essential oils are aromatic and volatile substances extracted from plants and characterized by antimicrobial activity. The aim of the present study was to evaluate the antibacterial activity (agar disc-diffusion method) of seven different bergamot essential oils (BEOs) on eight <em>Listeria monocytogenes</em> strains. Minimal inhibitory concentration (MIC) of most efficient BEOs was estimated. Extremely variable results for agar disc-diffusion method for <em>L. monocytogenes</em> strains were reported. One of the tested microorganisms resulted insensible to all the BEOs; 3 strains showed an inhibition from weak to null and the remaining 4 a variable susceptibility. Among the BEOs tested, one showed a strong activity against four pathogenic strains. Four BEOs revealed weak, moderate or null activity in all the 7 sensitive strains, while for two oils only a weak or no activity was reported. MIC values were 0.625 μL/mL for the most efficient BEO, 2.5 and 5 μL/mL for the other samples that showed moderate inhibition. Experiment results are significantly related to the strains tested (P&lt;0.01), rather than the BEO employed (P&gt;0.01). In conclusion, we can consider BEO as a natural technological hurdle for <em>Listeria monocytogenes</em> in combination with other preservation strategies. Finally, this study underlines the necessity to evaluate the antimicrobial activity of EOs on a significant strains number of the same bacteria.

scholarly journals Antibacterial Activity of Extract A. luteocarpa

2018 ◽  
Vol 1 (1) ◽  
pp. 9
Author(s):  
Tita Juwitaningsih ◽  
Destria Roza ◽  
Nora Susanti
Keyword(s):  
Antibacterial Activity ◽  
Positive Control ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Fresh Meat ◽  
Activity Test ◽  
Inhibition Zones ◽  
Paper Disc ◽  
Better Than

Meat is one of the food products that can easily be damaged, ( therefore it is necessary to preserve the process). therefore it needs to be done the process of preservation. In this study we studied the anti-bacterial activity of Alpinia luteocarpa extract against fresh meat rot bacteria, with paper disc diffusion method which is the standard recommended by the Clinical and Laboratory Standard Institute (CLSI,2012). The results of antibacterial activity test showed extract and fraction of A. luteocarpa able to inhibit bacterial growth B.cereus ATCC 21772   and. K.  pneumonia ATTC 13773. The bacteriostatic properties of the extract and fraction of A. luteocarpa against B. cereus ATCC 21772 and K. pneumonia ATTC 13773 were better than the positive control of chlorhexidine antibiotics (500 μg / mL) with inhibition zones 9-11 mm and 7-9 mm.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN DADAP SEREP (Erythrina lithosperma Miq.) TERHADAP PERTUMBUHAN Escherichia coli

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Asep Abdul Rahman ◽  
Ridwan Firmansyah ◽  
Lulu Setyabudi
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Abdominal Pain ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Activity Test ◽  
Paper Disc

Dadap Serep (Erythrina lithosperma Miq.) is one of the plants containing secondary metabolites in the form of alkaloids, flavonoids and tannins. Empirically dadap serep can be used to treat fever, abdominal pain, breast milk, prevent miscarriage, inflammation and cough. The aim of this research is to know the activity of ethanol extract in dadap serep (Erythrina lithosperma Miq.) in inhibiting growth of Escherichia coli ATCC 8939. The dadap serep leaves were extracted by maceration method using 96% ethanol solvent. The leaf thickness extract of dadap serep with concentration of 20%, 40%, 60% and 80% tested its antibacterial activity against growth of Escherichia coli ATCC 8939 using paper disc diffusion method. The result of concentration of leaf extract of dadap serep, obtained rendemen as much as 23,53%. Then the result of antibacterial activity test showed that leaf extract of dadap serep with concentration of 20% had no inhibition to growth of Escherichia coli ATCC 8939, while at concentration extract 40%, 60%, and 80% had inhibitory to growth of Escherichia coli ATCC 8939 with category weak resistor response.Keywords: Dadap serep (Erythrina lithosperma Miq.), Antibacterial activity, Escherichia coli ATCC 8939

scholarly journals Effects of Flower and Fruit Extracts ofMelastoma malabathricumLinn. on Growth of Pathogenic Bacteria:Listeria monocytogenes, Staphylococcus aureus, Escherichia coli,andSalmonella typhimurium

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Siti Nurhadis Che Omar ◽  
Janna Ong Abdullah ◽  
Khairul Anuar Khairoji ◽  
Sieo Chin Chin ◽  
Muhajir Hamid
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Listeria Monocytogenes ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Melastoma Malabathricum ◽  
Different Temperatures ◽  
Inhibition Zones ◽  
Inhibitory Activities

Melastoma malabathricumLinn. is a shrub that comes with beautiful pink or purple flowers and has berries-like fruits rich in anthocyanins. This study was carried out with the aim to evaluate the inhibitory activities of different concentrations of theM. malabathricumLinn. flower and fruit crude extracts againstListeria monocytogenesIMR L55,Staphylococcus aureusIMR S244,Escherichia coliIMR E30, andSalmonella typhimuriumIMR S100 using the disc diffusion method. The lowest concentrations of the extracts producing inhibition zones against the test microorganisms were used to determine their minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). In addition, the growth ofListeria monocytogenesIMR L55 andStaphylococcus aureusIMR S244 grown in medium supplemented with the respective extracts at different temperatures (4°C, 25°C, and 37°C) and pHs (4, 6, 7, and 8) was determined.

scholarly journals AKTIVITAS ANTIBAKTERI INFUSA DAUN LIDAH BUAYA (Aloe vera L) TERHADAP PERTUMBUHAN Staphylococcus aureus DAN Escherichia coli

2019 ◽  
Vol 6 (1) ◽  
pp. 33
Author(s):  
Yuni Widyastuti ◽  
Nia Yuliani ◽  
I.G.A. Manik Widhyastini
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Aloe Vera ◽  
Bacterial Count ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Escherichia Coli Bacteria

Antibacterial Activity of Infused Aloe Vera Leaf (Aloe Vera L) on Growth Of Stapylococcus aureus and Escherichia coliDisinfectants may act as antibacterials. Aloe vera (Aloe vera L) is one of the plants that is often used by the community as a medicinal plant that has many active compounds such as lignin, saponin and anthraquinone substances that act as antibacterial. This study aims to determine the antibacterial activity of Aloe vera leaf extracted with infusa method on the growth of Staphylococcus aureus and Escherichia coli bacteria. The research method is to see the reduction / reduction of bacterial count (% reduction) after 30 second and 60 second with disc diffusion method. The results showed decrease or decrease of bacteria count (% reduction) to Escherichia coli bacteria found in aloe leaf skin infected by 28,2% after 60 seconds. While decrease or decrease of bacteria amount (% reduction) to Staphyloccocus uareus bacteria infusa mixture of meat and skin of Aloe vera leaves of 27.5% after 60 seconds. Antibacterial test of aloe vera leaf aloe (Aloe veraL) of 100% concentration by disc diffusion method did not show any inhibition zone around cultures of test bacteria.Keywords: Aloe vera, antibacterial activity, Staphylococcus aureus, and Escherichia coli, Infusa.ABSTRAKDesinfektan dapat berfungsi sebagai antibakteri. Lidah buaya (Aloe vera L) merupakan salah satu tanaman yang sering digunakan masyarakat sebagai tanaman obat yang memiliki banyak senyawa aktif seperti lignin, saponin dan antrakuinon yaitu zat yang berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri daun lidah buaya (Aloe vera L) yang diekstrak dengan metode infusa terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Metode penelitian yaitu dengan melihat penurunan/pengurangan jumlah bakteri (% reduksi) setelah waktu 30 detik dan 60 detik dengan metode difusi cakram. Hasil penelitian menunjukkan penurunan atau pengurangan jumlah bakteri (% reduksi) terhadap bakteri Escherichia coli terdapat pada infusa kulit daun lidah buaya yaitu sebesar 28,2 % setelah waktu 60 detik.Sedangkan penurunan atau pengurangan jumlah bakteri (% reduksi) terhadap bakteri Staphyloccocus uareus terdapat pada infusa campuran daging dan kulit daun lidah buaya yaitu sebesar 27,5 % setelah waktu 60 detik. Uji antibakteri infusa daun lidah buaya (Aloe veraL) konsentrasi 100% dengan metode difusi cakram tidak menunjukkan adanya zona penghambatan disekitar biakan bakteri uji.Kata kunci : Lidah buaya, Aktivitas antibakteri, Staphylococcus aureus, dan   Escherichia coli,   Infusa.

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