scholarly journals Antibacterial Activity of Extract A. luteocarpa

2018 ◽  
Vol 1 (1) ◽  
pp. 9
Author(s):  
Tita Juwitaningsih ◽  
Destria Roza ◽  
Nora Susanti
Keyword(s):  
Antibacterial Activity ◽  
Positive Control ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Fresh Meat ◽  
Activity Test ◽  
Inhibition Zones ◽  
Paper Disc ◽  
Better Than

Meat is one of the food products that can easily be damaged, ( therefore it is necessary to preserve the process). therefore it needs to be done the process of preservation. In this study we studied the anti-bacterial activity of Alpinia luteocarpa extract against fresh meat rot bacteria, with paper disc diffusion method which is the standard recommended by the Clinical and Laboratory Standard Institute (CLSI,2012). The results of antibacterial activity test showed extract and fraction of A. luteocarpa able to inhibit bacterial growth B.cereus ATCC 21772   and. K.  pneumonia ATTC 13773. The bacteriostatic properties of the extract and fraction of A. luteocarpa against B. cereus ATCC 21772 and K. pneumonia ATTC 13773 were better than the positive control of chlorhexidine antibiotics (500 μg / mL) with inhibition zones 9-11 mm and 7-9 mm.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN DADAP SEREP (Erythrina lithosperma Miq.) TERHADAP PERTUMBUHAN Escherichia coli

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Asep Abdul Rahman ◽  
Ridwan Firmansyah ◽  
Lulu Setyabudi
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Abdominal Pain ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Activity Test ◽  
Paper Disc

Dadap Serep (Erythrina lithosperma Miq.) is one of the plants containing secondary metabolites in the form of alkaloids, flavonoids and tannins. Empirically dadap serep can be used to treat fever, abdominal pain, breast milk, prevent miscarriage, inflammation and cough. The aim of this research is to know the activity of ethanol extract in dadap serep (Erythrina lithosperma Miq.) in inhibiting growth of Escherichia coli ATCC 8939. The dadap serep leaves were extracted by maceration method using 96% ethanol solvent. The leaf thickness extract of dadap serep with concentration of 20%, 40%, 60% and 80% tested its antibacterial activity against growth of Escherichia coli ATCC 8939 using paper disc diffusion method. The result of concentration of leaf extract of dadap serep, obtained rendemen as much as 23,53%. Then the result of antibacterial activity test showed that leaf extract of dadap serep with concentration of 20% had no inhibition to growth of Escherichia coli ATCC 8939, while at concentration extract 40%, 60%, and 80% had inhibitory to growth of Escherichia coli ATCC 8939 with category weak resistor response.Keywords: Dadap serep (Erythrina lithosperma Miq.), Antibacterial activity, Escherichia coli ATCC 8939

scholarly journals IN VITRO ASSESSMENT OF ANTIBACTERIAL ACTIVITY OF STEM AND ROOT OF RAUWOLFIA SERPENTINA AGAINST DIFFERENT BACTERIA

2021 ◽  
Vol 12 (6) ◽  
pp. 87-90
Author(s):  
Virendra Vaishnav ◽  
Debasish Sahoo ◽  
Tanushree Chatterjee
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Development Program ◽  
Positive Control ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Rauwolfia Serpentina

Medicinal Plants are the good source of natural antimicrobial agents. The main aim of present study was to evaluate the antibacterial activity of stem and root of Rauwolfia serpentina against six microorganism, Powdered stem and root of plant were extracted with acetone, chloroform and methanol and streptomycin used as positive control. The antibacterial activity of Rauwolfia serpentine was detected by using disc diffusion method and agar well diffusion method on the following bacteria- Bacillus cereus, Staphylococcus aureus, Bacillus fusiformis, Escherichia coli, Pseudomonas aeruginosa and P. luminescens. The experiment reported that R. serpentina Root methanol extract shown 14.86 ± 1.11 highest antibacterial activity against Pseudomonas aeruginosa through well diffusion method. Whereas root chloroform recorded 13.46 ± 1.28 highest antibacterial activity against E. coli through disc diffusion method, maximum zone of inhibition 22.66±0.52 mm was found for the positive control, streptomycin through well diffusion method. Further studies should be undertaken to reveal the correct mechanism of action of antimicrobial effect to identify the active ingredients which can be used in drug development program.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK METANOL Acalypha hispida TERHADAP BAKTERI Shigella flexneri DAN Bacillus cereus IHB B 379

2020 ◽  
Vol 10 (2) ◽  
Author(s):  
Elvi Rusmiyanto P. Wardoyo ◽  
Devinda Ekarizky Diputri ◽  
Rikhsan Kurniatuhadi
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Methanol Extract ◽  
Shigella Flexneri ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Extract Concentration ◽  
Activity Test ◽  
Antibacterial Potential

AbstractAcalpha hispida flower commonly used as traditional medicine. The antibacterial potential of the A. hispida flower can be used to treat diarrhea and cancer. The purpose of this study was to determine the antibacterial activity and effective concentration of methanol extract of A. hispida flower against to S. flexneri and B. cereus. Sample used was flower of A. hispida from Kota Pontianak. The solvent used is metahnol. Antibacterial activity test using the disc diffusion method (Kirby-Bauer) with parameters based on grading levels  extract concentration in S. flexneri was 1.2;  1.3;  1.4;  1.5;  and 1.6 g/ml and B. cereus for 0.75;  0.80;  0.85;  0.90;  and 0.95 g/mL, 10% DMSO and ciprofloaxin as a comparison. the results of the research showed that the concentration of methanol extract of A. hispida flower which can effectively inhibit the growth of S. flexneri and B. cereus bacteria at 1.2 g / mL and 0.75 g / mL. The methanol extract of A. hispida flower contains compounds of alkaloids, flavonoids, tannins, phenols, steroids and terpenoids.Keywords : Acalypha hispida, Antibacterial, Shigella flexneri, Bacillus cereus AbstrakAcalypha hispida merupakan tanaman obat trardisional yang sudah dimanfaatkan masyarakat secara turun temurun untuk mengobati diare dan kanker. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri dan konsentrasi efektif ekstrak metanol bunga A. hispida terhadap bakteri Shigella flexneri dan Bacillus cereus. Sampel penelitian yang digunakan adalah bunga A. hispida yang diperoleh dari Kota Pontianak. Pelarut yang digunakan adalah methanol. Uji aktivitas antibakteri menggunakan metode difusi cakram (Kirby-Bauer) dengan perlakuan 5 taraf konsentrasi ekstrak pada bakteri S. flexneri sebesar 1,2; 1,3; 1,4; 1,5; dan 1,6 g/ml serta pada bakteri B. cereus IHB B 379 sebesar 0,75; 0,80; 0,85; 0,90; dan 0,95 g/mL, DMSO 10% serta sebagai pembanding digunakan ciprofloaxin. Hasil penelitian menunjukkan bahwa konsentrasi ekstrak methanol bunga A. hispida yang secara efektif dapat menghambat pertumbuhan bakteri S. flexneri dan B. cereus sebesar 1,2 g/mL dan 0,75 g/mL. Ekstrak methanol bunga A. hispida mengandung senyawa golongan alkaloid, flavonoid, tannin, fenol, steroid dan terpenoid.Kata kunci: Acalypha hispida, antibakteri, Shigella flexneri, Bacillus cereus

scholarly journals The Initial Antibacterial Activity Tests of Pliek U Oil and Pliek U: an Acehnese Traditional Food

2008 ◽  
Vol 2 (2) ◽  
Author(s):  
Nurliana Nurliana ◽  
Mirnawati Sudarwanto ◽  
Lisdar I. Sudirman ◽  
A. W. Sanjaya
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Diffusion Method ◽  
Traditional Food ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Agar Disc ◽  
Inhibition Zones ◽  
Initial Research

This initial research was intended to detect antibacterial activity of pliek u oil and pliek u. Pliek u oil consist of minyeuk simplah (MS) and minyeuk brok (MB), Pliek u consist of two kinds of solid waste namely wet pliek u (Ap1) and dry pliek u (Ap2). Pliek u was methanol extracted at concentration 10% (w/v). Pliek u oil and methanol extract of pliek u were evaluated for their antibacterial activity, against Bacillus subtilis and four strains of Enterophatogenic Escherichia coli (EPEC) employing agar disc diffusion method. No antibacterial activity was shown by MS. The MB exhibited a little effect 1-2 mm against bacterial tests. The results demonstrated that the higher antibacterial activity was shown by dry pliek u compare with wet pliek u with the inhibition zones from 6.67-10.33 mm and 6.00-7.33 mm respectively.

scholarly journals Uji Antibakteri Ekstrak Metanol Kulit Buah Manggis (garcinia mangostana l.) Terhadap Staphyllococcus aureus dan Escherechia coli

2017 ◽  
Vol 6 (1) ◽  
pp. 25
Author(s):  
Sujono Sujono ◽  
Anik Nuryati
Keyword(s):  
Antibacterial Activity ◽  
Methanol Extract ◽  
Diffusion Method ◽  
Test Results ◽  
Disc Diffusion Method ◽  
Garcinia Mangostana ◽  
Activity Test ◽  
Paper Disc ◽  
Growth Of Bacteria

Alfa mangostin, gamma-mangostin and xanthone group compounds are secondary metabolites contained in mangosteen (G. mangostana L.) which can be isolated from the fruit, bark, leaves and rind of mangosteen. All three compounds were shown to inhibit stronger against Mycobacterium tuberculosis. Alfa mangostin also active against Staphylococcus aureus and Enterococcus bacteria that are resistant to vancomisin and methicillin. Objective of this study to determine the antibacterial activity of methanol extract of mangosteen rind against Staphyllococcus aureus and Escherechia coli in vitro. Antibacterial activity test used is the diffusion wells and paper disc diffusion method. The test results obtained antibacterial activity of methanol extract of mangosteen rind can inhibit the growth of Staphyllococcus aureus at a concentration of 16%, but below its resistance zone diameter Cyprofloxacin. As against Escherichia coli, the methanol extract of mangosteen rind could not inhibit the growth of bacteria.

scholarly journals POTENSI EKSTRAK TUMBUHAN ANDALIMAN (Zanthoxylum acanthopodium DC.) SEBAGAI ANTIBAKTERI Staphylococcus aureus

Alotrop ◽  
2019 ◽  
Vol 2 (2) ◽  
pp. 133-139
Author(s):  
Oma Sepriani ◽  
Nurhamidah Nirhamidah ◽  
Dewi Handayani
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolite ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Activity Test ◽  
North Sumatra

The aims of this study is to determine the secondary metabolite compounds in Roots, stem bark, leaves and fruit of Andaliman (Zanthoxylum acanthopodium DC), and determine the most potent part of Andaliman plant extract in inhibiting the growth of Staphylococcus aureus bacteria. Andaliman plant taken in Simarpingan, Sipaholon village sub district of North Sumatra. Part of the Andaliman plantar are cleaned, cut into small pieces and then dried and mashed, samples that have been finely phytochemically tested and extracted. Testing of secondary metabolite compound by doing phytochemical screening of roots, stem bark, leaves and fruit of Andaliman, alkaloid test using meyers reagent, terpenoid test and steroid using TLC plate with eluent n-hexana : ethyl acetate with ratio 6:4, flavonoid test using soluton HCl and Mg band, saponin test using soap test, tannin test using ferro (III) chloride solution. Test of antibacterial activity using disc diffusion method. Phytochemical results showed that all parts of the plant andaliman contain secondary metabolite compounds namely alkaloids, flavonoids, terpenoids, tannins and also saponins. The result of antibacterial activity test of leaf extract of leaf and leaf which has the most potential as antibacterial of S.aureus is bark. Because it able to inhibit S.aureus growth with 11 mm inhibition zone at concentration 5x104 ppm, which is included in the strong category. In addition to skin of andaliman leaf stem is also potential as an antibacterial with a 10 mm inhibition zone at concentration 5x104 ppm, which is included in the medium category.

scholarly journals Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Somayeh Razmavar ◽  
Mahmood Ameen Abdulla ◽  
Salmah Binti Ismail ◽  
Pouya Hassandarvish
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Baeckea Frutescens ◽  
High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

scholarly journals Antibacterial Activity of Ethanol Extract of Spirulina platensis

2021 ◽  
Vol 7 (2) ◽  
pp. 47-51
Author(s):  
Prayoga Pannindrya ◽  
Mega Safithri ◽  
Kustiariyah Tarman
Keyword(s):  
Antibacterial Activity ◽  
Spirulina Platensis ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diffusion Method ◽  
Test Results ◽  
Nutritional Content ◽  
Disc Diffusion Method ◽  
Antibacterial Test ◽  
E Coli

Spirulina is a microalgae that has been widely used as various supplements and medicines because of its high nutritional content. The need for new antibacterial sources to solve the problem of antibiotic resistance, makes the potential of Spirulina as an antibacterial agent necessary to be researched. This study aims to analyze the antibacterial activity of ethanol extract of Spirulina platensis obtained from the Indonesian region against Staphylococcus aureus and Escherichia coli. Spirulina samples were macerated in ethanol solvent in a ratio of 1:10 (w / v). The antibacterial test used was the disc diffusion method with clindamycin positive control. Antibacterial test results showed that S. platensis in this study did not have antibacterial activity against E. coli and S. aureus.

scholarly journals EVALUATION OF ANTIBACTERIAL ACTIVITY OF A CYANOBACTERIUM SCYTONEMA SCHMIDTII GOM

2021 ◽  
pp. 77-79
Author(s):  
Janardhan Namdeo Nehul
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Axenic Culture ◽  
Soil Samples ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Air Blower

Scytonema schmidtii,a cyanobacterium was isolated from the collected soil samples from different locations of Ahmednagar district of Maharashtra state (India). Identification was carried out using morphological variation and taxonomical approaches according to Desikachary (1959) and Prescott (1962) .The axenic culture of Scytonema schmidtii was obtained by using the method recommended by Bolch and Blackburn (1996). The isolated Scytonema schmidtii was grown autotropically in BG-11 medium as described by Rippka et al.,(1979) and incubated at 30±2°c.After 25 days, biomass was harvested by filtration through double layered muslin cloth and dried using air blower. The biomass of this Scytonema schmidtii species was used for the assessment of antibacterial activity against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus flavus and Proteus mirabilis. The antibacterial activity was studied by disc diffusion method (Anonymous,1996).Methanol extract of Scytonema schmidtii showed the activity against all the tested bacterial strains.Maximum zone of inhibition (23±1.9 mm) was recorded with methanol extract of Scytonema schmidtii.

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