RADIOMODIFICATION OF CELL CULTURES OF LINE HELA BY CERIUM OXIDE NANOPARTICLES TO X-RAY IRRADIATION

Nanoparticles of cerium oxide CeO2 are important nanomaterials with remarkable properties for use in both industrial and non-industrial fields. In a general way, doping of oxide nanometric with transition metals improves the properties of nanoparticles. In this study, nickel- doped cerium oxide nanoparticles were synthesized from Stevia rebaudiana extract. Both doped and non-doped nanoparticles were characterized by X-ray diffraction, Field Emission Scanning Electron Microscopy, Energy Dispersive X-ray, Raman spectroscopy, and Vibrating-Sample Magnetometry analysis. According to X-ray diffraction, Raman and Energy Dispersive X-ray crystalline and single phase of CeO2 and Ni doped CeO2 nanoparticles exhibiting fluorite structure with F2g mode were synthesized. Field Emission Scanning Electron Microscopy shows that CeO2 and Ni doped nanoparticles have spherical shape and sizes ranging of 8 to 10 nm. Ni doping of CeO2 results in an increasing of magnetic properties. The enhancement of ultraviolet protector character via Ni doping of CeO2 is also discussed.

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Structure and Chemical Transformation in Cerium Oxide Nanoparticles Coated by Surfactant Cetyltrimethylammonium Bromide (CTAB): An X-ray Absorption Spectroscopic Study

The Journal of Physical Chemistry B ◽

10.1021/jp011520z ◽

2002 ◽

Vol 106 (18) ◽

pp. 4569-4577 ◽

Cited By ~ 37

Author(s):

Zhonghua Wu ◽

Jing Zhang ◽

Robert E. Benfield ◽

Yongfan Ding ◽

Didier Grandjean ◽

...

Keyword(s):

Spectroscopic Study ◽

Cerium Oxide ◽

Chemical Transformation ◽

Cetyltrimethylammonium Bromide ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

X Ray ◽

X Ray Absorption

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Revealing the Effect of Synthesis Conditions on the Structural, Optical, and Antibacterial Properties of Cerium Oxide Nanoparticles

Nanomaterials ◽

10.3390/nano11102596 ◽

2021 ◽

Vol 11 (10) ◽

pp. 2596

Author(s):

Nicusor Fifere ◽

Anton Airinei ◽

Marius Dobromir ◽

Liviu Sacarescu ◽

Simona I. Dunca

Keyword(s):

Cerium Oxide ◽

Diffuse Reflectance ◽

Diffuse Reflectance Spectroscopy ◽

Antibacterial Properties ◽

Morphological Characteristics ◽

Precipitation Method ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

X Ray ◽

Gap Energy

Cerium oxide nanoparticles were prepared by a precipitation method using Ce(IV) sulphate as precursor dispersed in glycerol with varying synthesis parameters such as temperature or precipitating agent. The structural and morphological characteristics of the obtained nanoparticles were investigated by X-ray diffraction, transmission electron microscopy, and diffuse reflectance spectroscopy. The crystallite size of the nanoparticles varied between 13 and 17 nm. The presence of Ce3+ and Ce4+ was proved by XPS data in the CeO2 samples and the conservation of the fluorite structure was evinced by X-ray diffractograms with a contraction of the lattice parameter, regardless of the size of the nanoparticle. From diffuse reflectance spectra, two band gap energy values for the direct transition were observed. Depending on the synthesis condition, the red shift of gap energy and the blue shift of Urbach energy with increasing content of Ce3+ were ascertained. The antibacterial tests revealed that the cerium oxide nanoparticles show good antimicrobial activity towards the common pathogens Escherichia coli and Staphylococcus aureus.

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Cerium Oxide Nanoparticles: Synthesis and Characterization for Biosafe Applications

Nanomanufacturing ◽

10.3390/nanomanufacturing1030013 ◽

2021 ◽

Vol 1 (3) ◽

pp. 176-189

Author(s):

Prathima Prabhu Tumkur ◽

Nithin Krisshna Gunasekaran ◽

Babu R. Lamani ◽

Nicole Nazario Bayon ◽

Krishnan Prabhakaran ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Cerium Oxide ◽

Ceo2 Nanoparticles ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

X Ray Diffraction ◽

X Ray ◽

Diphenyltetrazolium Bromide ◽

Scanning Electron

Due to its excellent physicochemical properties, cerium oxide (CeO2) has attracted much attention in recent years. CeO2 nanomaterials (nanoceria) are widely being used, which has resulted in them getting released to the environment, and exposure to humans (mostly via inhalation) is a major concern. In the present study, CeO2 nanoparticles were synthesized by hydroxide-mediated method and were further characterized by Scanning Electron Microscopy (SEM), Field Emission Scanning Electron Microscopy (FESEM), Energy Dispersive X-ray Spectroscopy (EDX), Fourier Transform Infrared Spectroscopy (FTIR), and X-ray Diffraction Spectroscopy (XRD). Human lung epithelial (Beas-2B) cells were used to assess the cytotoxicity and biocompatibility activity of CeO2 nanoparticles. 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and Live/Dead assays were performed to determine the cytotoxicity and biocompatibility of CeO2 nanoparticles. Generation of reactive oxygen species (ROS) by cerium oxide nanoparticles was assessed by ROS assay. MTT assay and Live/Dead assays showed no significant induction of cell death even at higher concentrations (100 μg per 100 μL) upon exposure to Beas-2B cells. ROS assay revealed that CeO2 nanoparticles did not induce ROS that contribute to the oxidative stress and inflammation leading to various disease conditions. Thus, CeO2 nanoparticles could be used in various applications including biosensors, cancer therapy, catalytic converters, sunscreen, and drug delivery.

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Protective Effects of Cerium Oxide Nanoparticles on MC3T3-E1 Osteoblastic Cells Exposed to X-Ray Irradiation

Cellular Physiology and Biochemistry ◽

10.1159/000443092 ◽

2016 ◽

Vol 38 (4) ◽

pp. 1510-1519 ◽

Cited By ~ 8

Author(s):

Cuifen Wang ◽

Eric Blough ◽

Xiaoniu Dai ◽

Omolola Olajide ◽

Henry Driscoll ◽

...

Keyword(s):

Cell Viability ◽

Cerium Oxide ◽

Molecular Mechanisms ◽

Ceo2 Nanoparticles ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Protective Effects ◽

X Rays ◽

Effective Prevention ◽

X Ray

Background/Aims: Exposure to ionizing radiation can result in bone damage, including decreased osteocyte number and suppressed osteoblastic activity. However, molecular mechanisms remain to be elucidated, and effective prevention strategies are still limited. This study was to investigate whether cerium oxide nanoparticles (CeO2 NP) can protect MC3T3-E1 osteoblast-like cells from damaging effects of X-ray irradiation, and to study the underpinning mechanism(s). Methods: MC3T3-E1, a osteoblast-like cell line, was exposed to X-ray irradiation and treated with different concentration of CeO2 nanoparticles. The micronucleus frequency was counted under a fluorescence microscope. Cell viability was evaluated using MTT assay. The effects of irradiation and CeO2 nanoparticles on alkaline phosphatase activity and MC3T3-E1 mineralization were further assayed. Results: We found that the ratio of micronuclei to binuclei was dose-dependently increased with X-ray irradiation (from 2 to 6 Gy), but diminished with the increased concentration of CeO2 NP treatment (from 50 to 100 nM). Exposure to X-rays (6 Gy) decreased cell viability, differentiation and the mineralization, but CeO2 NP treatment (100 nM) attenuated the deteriorative effects of irradiation. Both intracellular reactive oxygen species (ROS) production and extracellular H2O2 concentration were increased after X-ray irradiation, but reduced following CeO2 NP treatment. Similar to irradiation, exposure to H2O2 (10 µM) elevated the frequency of micronuclei and diminished cell viability and mineralization, while these changes were ameliorated following CeO2 NP treatment. Conclusions: Taken together, our findings suggest that CeO2 nanoparticles exhibit astonishing protective effects on irradiation-induced osteoradionecrosis in MC3T3-E1 cells, and the protective effects appear to be mediated, at least partially, by reducing oxidative stress.

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Cerium Oxide Nanoparticles Catalyst for the Oxidation of Methanol

Oriental Journal Of Chemistry ◽

10.13005/ojc/350510 ◽

2019 ◽

Vol 35 (5) ◽

pp. 1539-1545

Author(s):

Hossein Bayahia

Keyword(s):

Cerium Oxide ◽

Methanol Conversion ◽

Precipitation Method ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

X Ray Diffraction ◽

X Ray ◽

Oxidation Of Methanol ◽

Co Precipitation ◽

Cerium Oxide Catalyst

This study outlines the synthesis of cerium oxide nanoparticles, their characterization and their activity in the oxidation of methanol. A simple and easy co-precipitation method was used for the preparation of cerium oxide, without any added surfactants. The physicochemical properties of the sample were studied using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction (XRD). The morphology and size of the catalyst was studied using SEM. EDX confirms the element content of the synthesized cerium oxide. The structure of CeO2 was confirmed using XRD. Thus, the reported CeO2 was an active catalyst for methanol oxidation to form formaldehyde at a temperature range of 523–753K in the gas phase. At 753K, the cerium oxide catalyst gave 53% formaldehyde selectivity, 57% methanol conversion and 31% formaldehyde yields.

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Synergistic vs protective activity of cerium oxide nanoparticles (CNP) in gastrointestinal tumoral and non-tumoral cell cultures respectively when combined with cytotoxic drugs

Annals of Oncology ◽

10.1093/annonc/mdx261.026 ◽

2017 ◽

Vol 28 ◽

pp. iii20

Author(s):

Jose Vazquez Villa ◽

Amador Garcia Fuente ◽

Daniel Martinez Perez ◽

Rebeca Fernandez Fernandez ◽

García Alonso Francisco J ◽

...

Keyword(s):

Cerium Oxide ◽

Cell Cultures ◽

Cytotoxic Drugs ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Protective Activity ◽

Tumoral Cell

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EFFECT OF ANNEALING TEMPERATURE OF CERIUM OXIDE NANOPARTICLES STUDIED BY SMALL-ANGLE X-RAY SCATTERING

International Journal of Modern Physics B ◽

10.1142/s0217979205031432 ◽

2005 ◽

Vol 19 (15n17) ◽

pp. 2627-2632

Author(s):

X. JU ◽

L. X. RONG ◽

J. ZHANG ◽

B. Z. DONG

Keyword(s):

Cerium Oxide ◽

Small Angle ◽

Annealing Temperature ◽

Fractal Structure ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

X Ray ◽

X Ray Scattering ◽

Mass Fractal ◽

Ray Scattering

The evolution of microstructure of cerium oxide nanoparticles prepared in a sol-gel process was investigated by small-angle X-ray scattering (SAXS) measurements, in which the range of annealing temperature was from 298 K to 973 K in air. It was found that their scattering intensities increased dramatically and was proportional to h-a as the temperature went up to 873 K. Meanwhile, the average radius of gyration Rg, increased. This suggested that these cerium oxide nanoparticles have self-similar structure behavior or mass fractal structure. When the temperature was higher than 873 K, the feature of the SAXS spectra became identical to that of the sactterers with surface fractal structure rather than the mass fractal structure.

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