Occupational benzene exposure and the risk of genetic damage: a systematic review and meta-analysis

Abstract Background To systematically evaluate the influence of benzene exposure on the genetic damage index of workers, and to explore the influence of low concentration benzene exposure on workers’ genetic damage index using 3.25mg/m3 as the boundary value, in order to provide a basis for improved prevention and control of the harm from benzene exposure to the occupational population. Methods We conducted a search of five databases, including Pub Med, Web of Science,China National Knowledge Infrastructure(CNKI), Wan Fang Data and Chongqing VIP, to identify relevant articles up to December 25, 2018. Two researchers independently extracted and carefully evaluated the data according to the inclusion and exclusion criteria of the literature. The imported articles were managed by Endnote X7, and the data were extracted and sorted by Excel 2013. We utilized Stata 12.0 software to perform the meta-analysis in the present study. Results A total of 68 eligible articles were finally included for the synthetic analyses. The meta-analysis results showed that occupational benzene exposure led to significantly increased Micronucleus (MN) frequency, Sister chromatid exchange (SCE) frequency, Chromosome aberration (CA) frequency, Olive Tail moment (OTM), Tail moment (TM), Tail length (TL), and Tail DNA% (T DNA%) compared to the control group (P < 0.05), and the pooled effect value estimates were 1.36, 0.98, 0.76, 1.06, 0.96, 1.78, and 1.42, respectively. Subsequent analysis of the effect of low concentration benzene exposure on genetic damage found significantly increased MN frequency increased compared with the control group (P < 0.05). Conclusions Occupational benzene exposure can affect multiple genetic damage indicators. Even at an exposure concentration lower than 3.25mg/m3, benzene exposure has genotoxicity. These data provide an important scientific basis for the further revision of occupational disease prevention strategies in China. At the same time, increased attention should be focused on the health monitoring of the occupational population exposed to benzene, and health management should be strengthened to improve the health of the occupational population.

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Occupational benzene exposure and the risk of genetic damage: a systematic review and meta-analysis

10.21203/rs.2.10985/v1 ◽

2019 ◽

Author(s):

Yanhua Zhou ◽

Kun Wang ◽

Boshen Wang ◽

Yuepu Pu ◽

Juan Zhang

Keyword(s):

Meta Analysis ◽

Damage Index ◽

Tail Length ◽

Control Group ◽

Tail Moment ◽

Genetic Damage ◽

China National Knowledge Infrastructure ◽

Low Concentration ◽

Benzene Exposure ◽

Study Results

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Investigating Genetic Damage in Peripheral Lymphocytes of Radiation Workers at Al-Tuwaitha Site in Iraq Using Four Genetic End-Points

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2014.8.2.315 ◽

2014 ◽

Vol 8 (2) ◽

pp. 5-10

Author(s):

Amel J. Mutter ◽

Abdulsahib K. Ali ◽

Abdullah A. K. ◽

Haider Y. L. ◽

Ali H. F

Keyword(s):

Comet Assay ◽

Genetic Parameter ◽

Tail Length ◽

Control Group ◽

Tail Moment ◽

Genetic Damage ◽

Peripheral Lymphocytes ◽

Significant Difference ◽

Radiation Workers ◽

The Mean

The present study aims to use the biological techniques in a genotoxicity assessment of DNA damage in peripheral lymphocytes of radiation workers at Al-Tuwaitha site due to decommissioning to radioactive contamination as a result of work during January 2010 to December 2011. The subjects were divided into two groups: (i) 85 workers from radiation workers at Al-Tuwaitha site; (ii) 50 controls were matched non-smoking and no alcohol drink. Fresh blood samples were collected from the workers and controls. Four genetic parameter were studied using the micronucleus (MN) test, nuclear division index (NDI) test, the comet assay and hypoxanthine guanine phosphoribosyl transferase (HPRT) mutation assay. The results of the MN test showed that the average of MN per cell (Mean ± SE) in workers were 0.025 ± 0.0016 MN/ cells, which were significantly higher than those 0.010 ± 0.0006 MN/ cells in controls P< 0.01. While, the results of NDI test the average of NDI (Mean ± SE) in workers were 1.154 ± 0.0089 when compared with the control 1.322 ± 0.0117, which were significant increase p<0.01. It was found in the comet assay that the mean tail length (Mean ± SE) of radiation workers and controls were 17.69 ± 0.23 µm and 14.05 ± 0.13 µm, respectively. There was a significant difference between radiation workers and controls for mean tail length P < 0.01, but the difference between the mean tail moment (Mean ± SE) 14.22 ±0.21 of workers and mean tail moment 12.96± 0.15 of controls was not significant P> 0.01. Mean while, the results of the average of mutation frequency for HPRT were no significant differences rate for radiation workers compared with the control group P> 0.01. In conclusion, the results of our experiment suggest that the accumulation of genetic damage is detectable in peripheral lymphocytes of radiation workers at Al-Tuwaitha site. Also, the current results of frequency MN and NDI within of normal values according of the technical report of International Atomic Energy Agency (IAEA) No. 405, 2001.

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Efficacy and Safety of Topical Timolol Eye Drops in the Treatment of Myopic Regression after Laser In Situ Keratomileusis: A Systematic Review and Meta-Analysis

Journal of Ophthalmology ◽

10.1155/2015/985071 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 3

Author(s):

Xiaochen Wang ◽

Guiqiu Zhao ◽

Jing Lin ◽

Nan Jiang ◽

Qian Wang ◽

...

Keyword(s):

Meta Analysis ◽

Treated Group ◽

Laser In Situ Keratomileusis ◽

Control Group ◽

Eye Drops ◽

Efficacy And Safety ◽

China National Knowledge Infrastructure ◽

Knowledge Infrastructure ◽

Significant Difference

Aims. The aim of this study was to assess the efficacy and safety of timolol in the treatment of myopic regression after laser in situ keratomileusis (LASIK).Methods. We searched MEDLINE, CENTRAL, EMBASE, China National Knowledge Infrastructure (CNKI), and Chinese Biological Medicine (CBM) from the inception to July 2015 for relevant randomized controlled trials that examined timolol therapy for myopic regression. The methodological quality of the studies included was assessed using the Revman 5.3 software.Results. We included six clinical trials involving 483 eyes in this review, including 246 eyes in treated group and 237 eyes in controlled group. We observed statistically significant improvements on the postoperative SE in the 3 months. However, the change of CCT was not statistically different between the control group and the experimental group. There were fewer cases of IOP, UDVA, and CDVA in treated group having significant difference from the controlled group.Conclusions. Topical timolol could be an effective treatment for reduction of myopic regression especially the spherical errors after myopic LASIK. Further RCTs with larger sample sizes for these trials are warranted to determine the efficacy and limitation for myopic regression after LASIK.

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Nuclear Damage in Peripheral Erythrocytes of Cyprinus Carpio Exposed to Binary Mixture of Pesticides

Journal of Zoo Biology ◽

10.33687/zoobiol.002.01.1506 ◽

2019 ◽

Vol 2 (1) ◽

pp. 39-47

Author(s):

Faiza Ambreen ◽

Muhammad Javed

Keyword(s):

Dna Damage ◽

Cyprinus Carpio ◽

Peripheral Blood ◽

Damage Index ◽

Tail Length ◽

Time Dependent ◽

Lethal Concentration ◽

Static System ◽

Genetic Damage ◽

Long Duration

The present study was undertaken to examine the DNA damage in peripheral blood erythrocytes of Cyprinus carpio under the binary exposure of bifenthrin and chlorpyrifos by using single cell gel electrophoresis (SCGE). Limited efforts have been made to study the genotoxic effect for long duration period. Therefore, the present investigation was aimed to assess the genotoxicity of pesticide mixture to the freshwater carp, Cyprinus carpio at sub-lethal concentration exposure (33% LC50). At first 96-hr LC50 value of pesticide, the mixture was determined for Cyprinus carpio in a static system and then sub-lethal concentration was calculated and fish was exposed to this sub-lethal concentration of the mixture in glass aquaria for 70 days (five fortnights) at constant laboratory conditions. Peripheral blood erythrocytes were taken on a fortnightly basis for the time-dependent DNA damage assessment in-terms of percentage of damaged cells, genetic damage index and a cumulative tail length of comets. Concentration-dependent increase in the percentage of DNA damaged cells were observed up to a 4th fortnight, followed by a slight decrease in the 5th fortnight. Similarly, statistically significant time-dependent DNA damage was observed in terms of percentage of damaged cells, genetic damage index and a cumulative tail length of comets in treated fish (at 33% of LC50) as compared to control groups. The results supported the use of SCGE for evaluating the toxicity of pollutants which may be used as part of environmental monitoring programs.

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Effectiveness and safety of extracorporeal shock wave treatment for low back pain:a systematic review and meta-analysis of RCTs

10.21203/rs.3.rs-25516/v1 ◽

2020 ◽

Author(s):

Jinhui Ma ◽

Yan Yan ◽

Bailiang Wang ◽

Wei Sun ◽

Debo Yue ◽

...

Keyword(s):

Shock Wave ◽

Rating Scale ◽

Meta Analysis ◽

Post Treatment ◽

Mean Difference ◽

Control Group ◽

Low Back ◽

China National Knowledge Infrastructure ◽

Pain Scores ◽

Extracorporeal Shock Wave

Abstract Background Extracorporeal shock wave therapy (ESWT) has been widely used in musculoskeletal disorders. This meta-analysis was designed to assess the effectiveness and safety of ESWT for patients with low back pain (LBP). Methods Multiple electronic databases including Pubmed, Embase, Cochrane’s library, China National Knowledge Infrastructure (CNKI), and Wanfang Data were searched until December, 2019 to identify studies assessing the effectiveness and safety of EPSW for LBP. The prime outcome is pain intensity measured by Visual Analog Scale (VAS) or numeric rating scale (NRS). Other outcomes included functional status, quality of life, psychological outcomes measured by Oswestry Disability Index (ODI), as well as the adverse events. Mean differences were calculated for continuous outcomes, while odd ratios were calculated for binary outcomes. Revman 5.3 software was used for statistical analysis. Results Five randomized controlled trials (RCTs) were finally included in this meta-analysis. The pooled mean difference in post-treatment pain scores was −2.37 (P <0.0001), indicating that post-treatment pain scores was significantly higher by 2.37 in control group than in ESWT group. At a mean follow-up time of 4-6 weeks, the pooled mean difference in ODI scores was −14.10 (P <0.00001), indicating that the pooled mean difference of post-treatment ODI scores was 14.10 higher in control group than in ESWT group. Conclusions The use of focused ESWT is effective in alleviating pain and improving the general fuctional state for patients with LBP. However, more evidence was needed to verify its safety.

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Meta-Analysis Based on Clinical RCTs: The Effect of Molecular Epimerism on the Safety of Glycyrrhizic Acid

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/3869698 ◽

2020 ◽

Vol 2020 ◽

pp. 1-8

Author(s):

Hongdou Chen ◽

Fangfang Zheng ◽

Menglei Wang ◽

Xu Wang ◽

Qingqing Yang ◽

...

Keyword(s):

Glycyrrhizic Acid ◽

Meta Analysis ◽

Control Group ◽

China National Knowledge Infrastructure ◽

Clinical Safety ◽

Medical Network ◽

The Difference ◽

Clinical Control Trial ◽

Experimental Group ◽

Magnesium Isoglycyrrhizinate

Objective. To carry out the meta-analysis on the clinical safety of glycyrrhizic acid and the influencing factors between 18α-glycyrrhizinate (18α-GL) and 18β-glycyrrhizinate (18β-GL). Methods. Magnesium isoglycyrrhizinate injection was used as the representative preparation of 18α-GL, and compound glycyrrhizin injection was used as the representative preparation of 18β-GL. The clinical control trial of magnesium isoglycyrrhizinate injection and compound glycyrrhizin injection was searched in a computer, which was published from January 2006 to December 2019 on the databases such as PubMed, China National Knowledge Infrastructure (CNKI), China Science and Technology Journal Database (CSTJ), and Wanfang Medical Network (Wanfang Data). The data associated with adverse drug reactions (ADRs) were extracted. RevMan5.3 was used for statistical analysis. Results. Finally, 24 studies were included, and 2757 patients were involved, of which the experimental group was mainly treated with magnesium isoglycyrrhizinate, while the control group was mainly treated with compound glycyrrhizin. The results showed that the occurrence of ADRs was significantly lower in the experimental group than that in the control group, and the difference between two groups was statistically significant (RR = 0.26, 95% CI = (0.18, 0.38), P < 0.00001 ). There was no heterogeneity among the studies (I2 = 0%, P = 1.00 ). Conclusion. Compared with 18β-GL, 18α-GL had a lower incidence of adverse reactions and better clinical safety.

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Predictive Value and Correlation of Neuron-Specific Enolase for Prognosis in Patients with Coma: A Systematic Review and Meta-Analysis

European Neurology ◽

10.1159/000509801 ◽

2020 ◽

pp. 1-11

Author(s):

Wen Ye ◽

Yunliang Tang ◽

Xiaoyang Dong ◽

Gengfa Chen ◽

Yan Yan ◽

...

Keyword(s):

Predictive Value ◽

Meta Analysis ◽

Life Quality ◽

Neuron Specific Enolase ◽

Cochrane Library ◽

Control Group ◽

China National Knowledge Infrastructure ◽

Clinical Prognosis ◽

Standard Mean Difference ◽

Disturbance Of Consciousness

Objective: Coma is the most serious disturbance of consciousness, which affects the life quality of patients and increases the burden of their family. Studies to assess the prognostic value of neuron-specific enolase (NSE) in patients with coma have not led to precise, generally accepted prognostic rules. The study aims to assess the correlation between NSE and prognosis of coma and the predictive value of NSE for clinical prognosis. Methods: A search was conducted using PubMed, Web of Science, EMBASE, Cochrane Library, China National Knowledge Infrastructure (CNKI), and WanFang Data from the establishment time of databases to December 2019. This analysis included patients with coma, regardless of how long the coma was. In total, 26 articles were retrieved and included in the review. Results: The meta-analysis revealed the NSE concentration of patients with coma is significantly higher than that of the control group (standard mean difference = 0.88, 95% confidence interval [CI]: 0.63–1.12, p < 0.05). The pooled sensitivity and specificity of NSE in coma diagnosis was 0.5 (95% CI: 0.39–0.61) and 0.86 (95% CI: 0.71–0.94). Conclusions: The NSE concentration of patients with poor coma prognosis is significantly higher than that of the control group. The high NSE concentration is not necessarily a poor prognosis for coma, but low NSE concentration indicates a high probability of a good prognosis for coma.

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Sevoflurane and isoflurane genotoxicity in kidney cells of mice

Archives of Industrial Hygiene and Toxicology ◽

10.1515/aiht-2017-68-2941 ◽

2017 ◽

Vol 68 (3) ◽

pp. 228-235 ◽

Cited By ~ 2

Author(s):

Gordana Brozović ◽

Nada Oršolić ◽

Ružica Rozgaj ◽

Fabijan Knežević ◽

Anica Horvat Knežević ◽

...

Keyword(s):

Dna Damage ◽

Tail Length ◽

Control Group ◽

Kidney Cells ◽

Genetic Damage ◽

Volatile Anaesthetics ◽

Everyday Clinical Practice ◽

Dna Damage And Repair ◽

Mean Values ◽

Exposure Levels

AbstractThe aim of this study was to evaluate the DNA damage and repair in kidney cells of Swiss albino mice after repeated exposure to sevoflurane and isoflurane and compare their detrimental effects. We used the alkaline comet assay to establish the genetic damage and measured three parameters: tail length, tail moment, and tail intensity of comets. These parameters were measured immediately after exposure to the above mentioned inhalation anaesthetics, two hours, six hours, and 24 hours later and were compared with the control group. Mean values of all three parameters were significantly higher in experimental groups compared to the control group. DNA damage in kidney cells of mice exposed to sevoflurane increased continuously before it reached its peak 24 hours after exposure. Isoflurane induced the highest DNA damage two hours after exposure. Levels of DNA damage recorded 24 h after cessation of exposure to both tested compounds suggest that sevoflurane was slightly more genotoxic than isoflurane to kidney cells of mice. According to these results, the currently used volatile anaesthetics sevoflurane and isoflurane are able to damage DNA in kidney cells of mice. Such findings suggest a possibility for similar outcomes in humans and that fact must be taken into account in everyday clinical practice.

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Combination between Mifepristone and Methotrexate to Treat Ectopic Pregnancy: A Systematic Review Protocol

10.1101/2021.09.26.21264155 ◽

2021 ◽

Author(s):

Qiling Su ◽

Huiyan Feng ◽

Tian Tian ◽

Xiaoqian Liao ◽

Yunhui Li ◽

...

Keyword(s):

Ectopic Pregnancy ◽

Controlled Trial ◽

Meta Analysis ◽

Cochrane Library ◽

Secondary Outcome ◽

Control Group ◽

China National Knowledge Infrastructure ◽

Advantages And Disadvantages ◽

Randomized Controlled ◽

Outcome Index

Background: In recent years, the morbidity of ectopic pregnancy and the proportion of young and childless patients have increased year by year, which makes it important to early diagnose EP, effectively save patients' lives and furthest preserve their fertility. Methotrexate and mifepristone are most widely used in conservative treatments, however, there is no accurate conclusion about which therapy is better. Therefore, the aims in this meta-analysis are, on the one hand, to systematically analyze the efficacy of mifepristone combined with methotrexate in the treatment of ectopic pregnancy through existing studies, and to draw scientific conclusions. On the other hand, to fill the gap of relevant analysis in China and abroad, to evaluate the advantages and disadvantages of inclusion trials and propose improvement measures and scientific designing schemes. Methods: Six electronic databases will be searched, including PubMed, Embase, Cochrane Library, the China National Knowledge Infrastructure(CNKI), Chinese Science and Technology Periodical Database (VIP), and Wanfang Database (WF). Literature from October 2015 to October 2020 on randomized controlled trials will be searched, without any language or publication restriction. Search terms include mifepristone, methotrexate, ectopic pregnancy, and random (free word/synonym expansion). Included in a randomized controlled trial, the treatment group was treated with mifepristone combined with methotrexate, and the control group was treated with mifepristone alone. Revman 5.4 (provided by Cochrane) will be used to evaluate the quality of the literature, and the corresponding effect model will be selected to analyze the results. The cure rate will be the main outcome index, and the remaining outcome measures after literature inclusion will be the secondary outcome indexes. Result: Only when we finish this meta-analysis can we get the result. Discussion: The results of this study will provide reliable evidence for the efficacy of mifepristone combined with methotrexate therapy in the treatment of ectopic pregnancy.

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Prophylactic Donor Lymphocyte Infusion for Relapse Prevention; A Meta-Analysis

Blood ◽

10.1182/blood-2019-121379 ◽

2019 ◽

Vol 134 (Supplement_1) ◽

pp. 2017-2017

Author(s):

Kittika Poonsombudlert ◽

Jakrin Kewcharoen ◽

Chattip Prueksapraopong ◽

Limpruttidham Nath

Keyword(s):

Relapse Prevention ◽

Meta Analysis ◽

Hematologic Malignancies ◽

Cochrane Library ◽

Control Group ◽

Cell Transplant ◽

Ongoing Research ◽

Hematopoietic Stem ◽

Study Results ◽

Risk Of Relapse

Objective: relapsed hematologic malignancies after standard hematopoietic stem cell transplant (HSCT) is one of the most challenging diseases to treat, therefore ongoing research are aiming at relapse prevention and minimizing the transplant related side effects. Prophylactic donor lymphocytes (pDLI) had been proposed as a valuable strategy for relapse prevention but early study results had been discouraging due to association with acute graft-versus-host disease (aGVHD). We attempted to establish the efficacy of pDLI for relapse prevention and define its association with aGVHD. Method: we searched for titles of articles in MEDLINE (PubMed), Cochrane library, EMBASE database and Google Scholar in May 2019 that evaluated the association between pDLI, relapse and aGVHD. We conducted a random effect meta-analysis of 9 studies involving a total of 748 participants and reported the pooled odd ratio (OR) for incidence of relapse and aGVHD between patients in the pDLI group and the control group. Result: we found a significantly decreased risk of relapse in the pDLI group versus the control group with a pooled OR of 0.42, 95% CI 0.30-0.58, I2=0% while there is no significantly increased in the risk of aGVHD with a pooled OR of 0.98, 95% CI 0.56-1.72, I2=0.8% Conclusion: there is significantly decreased risk of relapse in the pDLI group compared to the control group but there is no statistically significant increased risk of aGVHD. We concluded that pDLI is a valuable method of post-transplant relapse prevention for hematologic malignancies and further research is encouraged in a larger population. Disclosures No relevant conflicts of interest to declare.

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