scholarly journals Bottleneck Removal of Paclitaxel Biosynthetic Pathway By Overexpression of DBTNBT Gene Under Methyl- β- Cyclodextrin and Coronatine Elicitation in Taxus Baccata L.

2021 ◽  
Author(s):  
Kimia Kashani ◽  
Mohammad Sadegh Sabet ◽  
Mokhtar Jalali Javaran ◽  
Ahmad Moieni
Keyword(s):  
Large Scale ◽  
Expression Level ◽  
35S Promoter ◽  
Taxus Baccata ◽  
Scale Production ◽  
Limited Scale ◽  
Cancer Types ◽  
Transient Overexpression ◽  
Near Future ◽  
Rate Limiting

Abstract Paclitaxel is a highly functionalized diterpenoid that is broadly used for the treatment of several cancer types. This valuable specialized metabolite naturally exists in the inner bark of Taxus species in low amounts. The limited-scale production of paclitaxel in Taxus cell cultures has necessitated the use of several elicitors. Recently, methyl-β-cyclodextrin (CD) and coronatine (COR) have been considered to be highly effective elicitors in producing plant specialized metabolites. Given the limited production of paclitaxel due to the rate limiting enzymes' function, bottleneck removal is conducive to the production of more significant amounts of paclitaxel. In the present study, the full length of DBTNBT coding sequence (CDS), as one of the paclitaxel pathway bottlenecks, was integrated downstream of the CaMV 35S promoter (pCAMBIA1304-DBTNBT) and transiently expressed in Taxus baccata leaves via Agrobacterium tumefaciens and vacuum infiltration method. Paclitaxel production and the expression level of several involved genes were evaluated through different treatments. The transient overexpression of the DBTNBT gene, associated with dual elicitation, resulted in 7.4-fold more paclitaxel production compared with the no-inoculation/no-elicitation control. These ratios were 2.1 and 1.8 in the CD+COR and pCAM treatments, respectively. Among T13αH, T14βH, DBAT, BAPT, DBTNBT, and ABC genes, the most increased expression level belonged to the DBTNBT gene, followed by ABC and BAPT genes. It seems as though in the near future, bottleneck removal could be used on a large scale in Taxus metabolic engineering, resulting in the relative removal of some other bottlenecks and an increase in the final paclitaxel production.

scholarly journals Advances in the Metabolic Engineering of Escherichia coli for the Manufacture of Monoterpenes

Catalysts ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 433 ◽  
Author(s):  
Si-si Xie ◽  
Lingyun Zhu ◽  
Xin-yuan Qiu ◽  
Chu-shu Zhu ◽  
Lv-yun Zhu
Keyword(s):  
Large Scale ◽  
Metabolic Flux ◽  
Process Development ◽  
Pathway Engineering ◽  
Scale Production ◽  
Future Studies ◽  
E Coli ◽  
Large Scale Production ◽  
Rate Limiting ◽  
Dependent Pathway

Monoterpenes are commonly applied as pharmaceuticals and valuable chemicals in various areas. The bioproduction of valuable monoterpenes in prokaryotic microbial hosts, such as E. coli, has progressed considerably thanks to the development of different outstanding approaches. However, the large-scale production of monoterpenes still presents considerable limitations. Thus, process development warrants further investigations. This review discusses the endogenous methylerythritol-4-phosphate-dependent pathway engineering and the exogenous mevalonate-dependent isoprenoid pathway introduction, as well as the accompanied optimization of rate-limiting enzymes, metabolic flux, and product toxicity tolerance. We suggest further studies to focus on the development of systematical, integrational, and synthetic biological strategies in light of the inter disciplines at the cutting edge. Our review provides insights into the current advances of monoterpene bioengineering and serves as a reference for future studies to promote the industrial production of valuable monoterpenes.

scholarly journals Propagation of New Chestnut Rootstocks with Improved Resistance Phytophthora cinnamomi – New Cast Rootstocks

2020 ◽  
Vol 28 (1) ◽  
pp. 15-29
Author(s):  
Patrícia Fernandes ◽  
Andreia Amaral ◽  
Belén Colavolpe ◽  
Daniela Balonas ◽  
Maria Serra ◽  
...  
Keyword(s):  
Production System ◽  
Large Scale ◽  
Phytophthora Cinnamomi ◽  
Scale Production ◽  
Ex Vitro ◽  
Continuous Growth ◽  
Biotic Stresses ◽  
Large Scale Production ◽  
Near Future

Nowadays there is a great demand for chestnut rootstocks with improved resistance to Phytophthora cinnamomi, the causing agent of ink disease/root rot. The majority of genotypes that are being commercialized and used in the new plantations, were selected from the breeding programs of the XX century. The climate scenarios have been changing throughout the years, therefore the selection of new genotypes, better adapted to the current conditions of soil and climate, together with an effective and expedite production system, are essential to fulfill the needs of the market on better propagation materials. The INIAV’s chestnut breeding program, established in 2006, aims the improvement of resistance of chestnut to biotic stresses. By taking advantage of micropropagation, this program developed a pipeline to produce new hybrid varieties with improved resistance to P. cinnamomi. Micropropagation helps to overcome the recalcitrance that chestnut tissues present to traditional clonal propagation techniques. This work describes the pipeline of production of these hybrids and characterizes the plants from in vitro to ex vitro conditions. The pipeline allows the development of healthy plants capable of robust and continuous growth in ex vitro conditions. Nowadays these hybrids are being multiplied in a large scale production system, to be released to the market in the near future.

Large-scale production of carbon nanotubes and their applications

2006 ◽  
Vol 78 (9) ◽  
pp. 1703-1713 ◽  
Author(s):  
Morinobu Endo ◽  
Takuya Hayashi ◽  
Yoong-Ahm Kim
Keyword(s):  
Carbon Nanotubes ◽  
Medical Devices ◽  
Graphene Layer ◽  
Large Scale ◽  
Recent Progress ◽  
Scale Production ◽  
Practical Applications ◽  
Electrochemical Systems ◽  
Large Scale Production ◽  
Near Future

Carbon nanotubes consisting of a rolled graphene layer built from sp2 units have attracted the imagination of scientists as 1D macromolecules. Here, we will describe the recent progress on selective synthesis of various carbon nanotubes through the judicious control of synthetic conditions and their practical applications of these carbon nanotubes in the fields of electrochemical systems, nanocomposites, and medical devices. It is envisaged that carbon nanotubes will play an important role in the development of nanotechnology in the near future.

scholarly journals Modern high-resistance alloy steel used in railway transport

2017 ◽  
Vol 171 (4) ◽  
pp. 250-254
Author(s):  
Włodzimierz STAWECKI ◽  
Maciej BAJERLEIN ◽  
Paweł DASZKIEWICZ
Keyword(s):  
Large Scale ◽  
Technology Development ◽  
Rail Transport ◽  
Scale Production ◽  
Railway Transport ◽  
Rail Vehicles ◽  
Large Scale Production ◽  
Resistance Alloy ◽  
The One ◽  
Near Future

The article presents an overview of modern steel used in rail transport. Directions for the development of materials, technologies and constructions used in the manufacture of rail vehicles are, on the one hand, determined by the technical and economic requirements of industry and, on the other, by the continued emphasis of environmentalists on reducing fuel consumption and CO2 emissions. This generates innovation in the use of modern materials, processing methods and new design solutions and results in reduced vehicle weight. More bold forecasts today show that at the current pace of technology development, in the near future the mass of the vehicle will reduce by an average of 30%. Observing the trends and pace of rail transport development, it can be predicted that existing restrictions on the use of modern materials, such as high prices, technological conditions and difficult automation of large-scale production, can be predicted.

scholarly journals Thermodynamic insights into the structural basis governing the donor substrate recognition by human β1,4-galactosyltransferase 7

2009 ◽  
Vol 418 (3) ◽  
pp. 605-614 ◽  
Author(s):  
Franck Daligault ◽  
Sophie Rahuel-Clermont ◽  
Sandrine Gulberti ◽  
Manh-Thong Cung ◽  
Guy Branlant ◽  
...  
Keyword(s):  
Large Scale ◽  
Substrate Recognition ◽  
Structural Basis ◽  
Titration Calorimetry ◽  
Scale Production ◽  
Binding Studies ◽  
Donor Substrate ◽  
Large Scale Production ◽  
Rate Limiting

Human β1,4-GalT (galactosyltransferase)7 is involved in the biosynthesis of the tetrasaccharide linker protein region (GlcAβ1→3Galβ1→3Galβ1→4Xylβ1) (where GlcA is glucuronic acid and Xyl is xylose) of proteoglycans, by catalysing the transfer of Gal (galactose) from the uridine 5′-diphosphogalactose to a Xyl residue. This reaction is rate-limiting in glycosaminoglycan biosynthesis. In the present study, we established a large-scale production system of β1,4-GalT7 fused with the maltose-binding protein to study substrate recognition. Calorimetric binding studies showed that the binding of the donor substrate UDP-Gal largely promoted binding of the acceptor substrate. To identify the structural basis governing substrate recognition, we used a fragment-based approach involving the artificial breakdown of the donor substrate into smaller fragments and characterization of their respective binding to the enzyme by isothermal titration calorimetry. The β-phosphate, and to a lesser extent the α-phosphate, largely contributed to the binding energy. However, the uridine moiety was found to be essential for the optimal positioning of the donor substrate within the binding site. Unexpectedly, the contribution of the Gal moiety in substrate recognition was found to be negligible. Indeed, UDP-Gal, but also various UDP-sugars, could bind to β1,4-GalT7. Surprisingly, in contrast with other GalTs, soluble β1,4-GalT7 was able to transfer Glc (glucose), Xyl and, to a lesser extent GlcA and GlcNAc (N-acetyl glucosamine), to acceptor sugars, whereas UDP-Man (mannose) and UDP-GalNAc (N-acetyl galactosamine) were not substrates.

AGRICULTURAL PRODUCTION OF CA DONG PEOPLE IN RESETTLEMENT AREA OF TRANH RIVER HYDROPOWER NUMBER 2: CURRENT SITUATION AND IMPACT FACTORS

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Bùi Thị Bích Lan
Keyword(s):  
Social Relations ◽  
Agricultural Production ◽  
Large Scale ◽  
Transition Process ◽  
Impact Factors ◽  
Small Scale ◽  
Scale Production ◽  
Technical Application ◽  
Production Models ◽  
Role Of The State

In Vietnam, the construction of hydropower projects has contributed significantly in the cause of industrialization and modernization of the country. The place where hydropower projects are built is mostly inhabited by ethnic minorities - communities that rely primarily on land, a very important source of livelihood security. In the context of the lack of common productive land in resettlement areas, the orientation for agricultural production is to promote indigenous knowledge combined with increasing scientific and technical application; shifting from small-scale production practices to large-scale commodity production. However, the research results of this article show that many obstacles in the transition process are being posed such as limitations on natural resources, traditional production thinking or the suitability and effectiveness of scientific - technical application models. When agricultural production does not ensure food security, a number of implications for people’s lives are increasingly evident, such as poverty, preserving cultural identity, social relations and resource protection. Since then, it has set the role of the State in researching and building appropriate agricultural production models to exploit local strengths and ensure sustainability.

I. S. A. Baud. Forms of Production and Women's Labour: Gender Aspects of Industrialisation in India and Mexico. New Delhi: Sage Publications. 1992. 321 pp.Hardbound. Price: Indian Rs 295.00.

1993 ◽  
Vol 32 (1) ◽  
pp. 129-131
Author(s):  
Naureen Talha
Keyword(s):  
Large Scale ◽  
Indian Society ◽  
New Delhi ◽  
Scale Production ◽  
Female Labour ◽  
Self Employment ◽  
Commodity Production ◽  
Large Scale Production ◽  
Mexican Society ◽  
Small Production

The literature on female labour in Third World countries has become quite extensive. India, being comparatively more advanced industrially, and in view of its size and population, presents a pictures of multiplicity of problems which face the female labour market. However, the author has also included Mexico in this analytical study. It is interesting to see the characteristics of developing industrialisation in two different societies: the Indian society, which is conservative, and the Mexican society, which is progressive. In the first chapter of the book, the author explains that he is not concerned with the process of industrialisation and female labour employed at different levels of work, but that he is interested in forms of production and women's employment in large-scale production, petty commodity production, marginal small production, and self-employment in the informal sector. It is only by analysis of these forms that the picture of females having a lower status is understood in its social and political setting.

Cost- und Monitoring-Projekt: Zwei neue forstliche Meinungsumfragen im Vergleich | The Cost- and Monitoring-Projects: A Comparison of Two New Forestry Opinion Polls

2000 ◽  
Vol 151 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Stephan Wild-Eck ◽  
Willi Zimmermann
Keyword(s):  
Large Scale ◽  
Forest Policy ◽  
Forest Economics ◽  
Positive Attitudes ◽  
Federal Institute ◽  
Swiss Confederation ◽  
Institute Of Technology ◽  
The Cost ◽  
Near Future ◽  
Opinion Polls

Two large-scale surveys looking at attitudes towards forests, forestry and forest policy in the second half ofthe nineties have been carried out. This work was done on behalf of the Swiss Confederation by the Chair of Forest Policy and Forest Economics of the Federal Institute of Technology (ETH) in Zurich. Not only did the two studies use very different methods, but the results also varied greatly as far as infrastructure and basic conditions were concerned. One of the main differences between the two studies was the fact that the first dealt only with mountainous areas, whereas the second was carried out on the whole Swiss population. The results of the studies reflect these differences:each produced its own specific findings. Where the same (or similar) questions were asked, the answers highlight not only how the attitudes of those questioned differ, but also views that they hold in common. Both surveys showed positive attitudes towards forests in general, as well as a deep-seated appreciation ofthe forest as a recreational area, and a positive approach to tending. Detailed results of the two surveys will be available in the near future.

Solid Lipid Nanoparticles: A Promising Drug Delivery Technology

2009 ◽  
Vol 2 (2) ◽  
pp. 509-516
Author(s):  
S. Pragati ◽  
S. Kuldeep ◽  
S. Ashok ◽  
M. Satheesh
Keyword(s):  
Drug Delivery ◽  
Solid Lipid Nanoparticles ◽  
Large Scale ◽  
Colloidal Particles ◽  
Scale Up ◽  
Lipid Nanoparticles ◽  
Scale Production ◽  
Research Activity ◽  
New Approach ◽  
Solid Lipid

One of the situations in the treatment of disease is the delivery of efficacious medication of appropriate concentration to the site of action in a controlled and continual manner. Nanoparticle represents an important particulate carrier system, developed accordingly. Nanoparticles are solid colloidal particles ranging in size from 1 to 1000 nm and composed of macromolecular material. Nanoparticles could be polymeric or lipidic (SLNs). Industry estimates suggest that approximately 40% of lipophilic drug candidates fail due to solubility and formulation stability issues, prompting significant research activity in advanced lipophile delivery technologies. Solid lipid nanoparticle technology represents a promising new approach to lipophile drug delivery. Solid lipid nanoparticles (SLNs) are important advancement in this area. The bioacceptable and biodegradable nature of SLNs makes them less toxic as compared to polymeric nanoparticles. Supplemented with small size which prolongs the circulation time in blood, feasible scale up for large scale production and absence of burst effect makes them interesting candidates for study. In this present review this new approach is discussed in terms of their preparation, advantages, characterization and special features.

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