scholarly journals Impact of 2nd Wave of COVID-19 Related Lockdown on Coastal Water Quality at Diu, Western Coast of India and Role of Total Alkalinity on Bacterial Loads

Author(s):  
Atanu Kumar Panja ◽  
Sonpal Vasavdutta ◽  
Tarini Prasad Sahoo ◽  
Ambika Hemant Shinde ◽  
Ravikumar Bhagawan Thorat ◽  
...  

Abstract A detailed coastal water monitoring near Diu coast, western part of India was performed from October, 2020 to May, 2021 covering the 2nd lockdown time. Average monthly fluctuation from 7 different sampling stations of total 9 physico-chemical parameters such as pH, salinity, turbidity, nitrite (NO2), nitrate (NO3), ammonia (NH3), phosphate (PO4), total alkalinity and silicate were recorded. Initially, Mann-Kendall trend test for all the 9 parameters showed non-zero trend, which may be either linear or non-linear. During 2nd lockdown period, there was a fluctuation of value for parameters like pH, salinity, nitrate, nitrite and phosphate. Average total bacterial count and differential bacterial count also gradually decreased from March, 2021 sampling. Principal component analysis (PCA) plot covering all the physico-chemical parameters as well as the differential bacterial count showed a distinct cluster of all bacterial count with total alkalinity value. Subsequently, mathematical equation was formulated between total alkalinity value and all differential bacterial count. Upto our knowledge this is the first report where mathematical equation was formulated to obtain value of different bacterial load based on the derived total alkalinity value of the coastal water samples near Diu, India.

2020 ◽  
Vol 29 (1) ◽  
pp. 23-39
Author(s):  
R.Y. Oladunjoye ◽  
O.O. Fafioye ◽  
T.D. Solola ◽  
U.M. Ogbu ◽  
T.S. Oladeji

Physico-chemical parameters and microbial contents of Ogun River at Magodo area, Lagos were investigated in relation to its safety for consumption. Water samples were weekly collected between August and December, 2016 at three equi-distance intervals for analysis on physico-chemical parameters, total bacterial count and biochemical tests following standard recommended procedures. Mean concentrations of the physico-chemical parameters were found in ranges for pH (6.68 ± 0.15 - 6.86 ± 0.21), temperature (26.78 ± 0.14 – 26.92 ± 0.31), electrical conductivity (355.40 ± 101.93 – 456.40 ± 115.77), total dissolved solids (166.80 ± 48.08 - 215.00 ± 54.29), dissolved oxygen (7.06 ± 0.01 - 7.08 ± 0.02), biochemical oxygen demand (25.59 ± 0.03 - 25.64 ± 0.01), chemical oxygen demand (98.75 ± 0.03 - 98.84 ± 0.02), total hardness (345.80 ± 1.28 - 349.40 ± 3.87), total alkalinity (9.28 ± 0.02 - 9.33 ± 0.02), nitrate (0.27 ± 0.00 - 0.28 ± 0.00), sulphate (0.55 ± 0.05 - 0.62 ± 0.00) and chloride (68.84 ± 0.77 - 74.97 ± 1.23). Total colony forming unit (Cfu) of bacteria in the river was found highest in descending order Centrimide Agar (CA) > Mac-Conkey Agar (MCA) > Plate Count Agar (PCA) > Mannitol Salt Agar (MSA) at the first and second serial dilution respectively. Biochemical tests showed the presence of Staphylococcus species, Escherichia coli, Pseudomonas aeruginosa, Proteus sp., Salmonella sp. and Baccillus sp. in the river. Variations in the microbial load and physico-chemical parameters in Ogun River as a result of different human activities around the river was found within the standard limits which makes the River safe for human consumption. Key words: River Ogun, microbial load, bacterial count, biochemical tests Trop.


Author(s):  
MI Hoque ◽  
AKMF Rahman ◽  
MA Mansur ◽  
S Rahman

An experiment was carried out on the effects of periphyton on monoculture of Thai sharputi, Puntius gonionotus at the Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh during 7th August to 8th November. In treatment-1 bamboo poles were used as artificial substrate for periphyton production and in treatment-2 there was no artificial substrate (control). Each of the six ponds was stocked with 150 fingerlings of average size 6.41 cm and 3.60 g. The ponds were fertilized fortnightly with manure (cow dung) at a rate of 10 kg decimal-1, urea 60 g decimal-1 and triple super phosphate 90 g decimal-1. During the experimental period, the ranges of physico-chemical parameters viz. air temperature (31.0-35.50C), water temperature (29-320C), water depth (0.56-0.84 m), transparency (32-63 cm), dissolved oxygen (3.5-7.8 mg L-1), pH (6.8-7.9), total alkalinity (44-92 mg L-1), free CO2 (1.5-4.0 mg L-1), phosphate-phosphorus (0.31-1.07 mg L-1) and nitrate-nitrogen (1.12-2.30 mg L-1) were within the productive range and more or less similar in the ponds under treatments-1 and 2. Among the observed biological parameters, there were 35 genera of phytoplankton composed of five groups and 13 genera of zooplankton composed of four groups in the experimental ponds. Thirty three genera under the groups of Bacillariophyceae, Chlorophyceae, Cyanophyceae and Euglenophyceae formed the periphyton on bamboo poles in the experimental ponds. Net fish production of the ponds with periphyton under treatment-1 was about 1.5 times higher than those of the ponds without periphyton (treatment-2). By analysis of variance, it was found that the net fish production of Thai sharputi under treatment-1 was significantly higher than that under treatment-2 (p< 0.05). Finally, it can be concluded that periphyton is one of the preferable food item of Thai sharputi and it is also suggested that growth and production of Thai sharputi can be increased if arrangement is made for periphyton production. Int. J. Agril. Res. Innov. & Tech. 8 (2): 13-23, December, 2018


2020 ◽  
Vol 26 (2) ◽  
Author(s):  
Vijiyata Singh Rathour ◽  
Vijay Kumar Yadav

Bioassay experiments were carried out in order to evaluate values of physico-chemical parameters of the cultured pond water for induced pearl production in Lamellidens marginalis. The pond was located at Botanical garden of Bipin Bihari P.G. College, Jhansi. The parameters included for this study were Colour, Temperature, Transparency, pH, Free CO2 , DO, Total Hardness, Calcium, Magnesium and Total Alkalinity of cultured pond water and to maintain them for the healthy growth of mussels during the whole culture period for obtaining designer pearls.


Author(s):  
U. K. Asemota ◽  
M. D. Makut ◽  
S. O. Obiekezie ◽  
J. E. Owuna ◽  
M. O. Adamu

The aim of this study was to determine the antibiogram of bacterial isolates from Tympanotonus fuscatus var. radula sold in markets in Nasarawa State. Nigeria. Samples of Tympanotonus fuscatus var. radula (periwinkles) were bought from soup ingredient sellers at different sale locations in Keffi, Masaka and Orange markets and were analyzed using standard bacteriological methods. The bacterial isolates were identified using morphological, cultural and biochemical techniques. The total bacteria count varied from 1.18–3.20 x 108 CFU/g for the raw samples while the total bacterial count for the boiled samples varied from 0–1.57 x 108 CFU/g. Periwinkle samples with shells from Masaka market had the highest bacterial load with a mean total bacterial count of 2.94 x 10⁸ CFU/g and mean total coliform count of 2.80 x 10⁶ CFU/g. Raw periwinkle samples with shells had a higher bacterial load than samples without shells. There was also a drastic reduction in the bacterial load in the periwinkle samples after boiling under laboratory conditions. The bacteria isolated were Bacillus spp. and Staphylococcus aureus were the Gram-positive bacteria isolated. Enterobacter spp., Escherichia coli, Salmonella spp., Pseudomonas spp., Serratia spp. and Proteus spp. The most frequently occurring gram positive bacteria was Escherichia coli with an isolation frequency of 6(24%), the least frequently occurring was Bacillus spp., 8(32)%. Antibiotic susceptibility test showed that all the gram negative organisms exhibited sensitivity to ciprofloxacin: Escherichia coli (32 mm), Enterobacter spp. (41.5 mm), Proteus spp. (40.0 mm), Salmonella spp. (37.0 mm), Serratia spp. (26.0 mm), Pseudomonas spp. (23.0 mm). All the gram negative organisms showed marked resistance to vancomycin: Escherichia coli (12.0 mm), Enterobacter spp. (10.0 mm), Proteus spp. (11.0 mm), Salmonella spp. (5.0 mm), Serratia spp. (10.0 mm) and Pseudomonas spp. (4.5 mm).


2018 ◽  
Vol 30 ◽  
pp. 129-140
Author(s):  
Ganesh Bahadur Thapa ◽  
Joydeb Pal

Itahari Sub-metropolitan pond is located in the centre of the city. Influence of variations in physico-chemical parameters on aquatic flora and fauna especially in fishes brought strong stress on fishes and increases the incidence of diseases leading to high mortality. Due to lack of adequate knowledge about it, productivity of ponds is very low. In the present study, maximum and minimum variations and correlation between different physico-chemical parameters - air temperature, water temperature, pH, turbidity, total dissolved solids, dissolved carbon dioxide, ammonia, dissolved oxygen, biological oxygen demand, chloride, total alkalinity, total hardness and phosphate were taken into consideration. The water quality of fish ponds were normal except high fluctuation of chloride 4.0 - 44.87mg/l and ammonia 0.48-13.6 mg/l during manuaring period and casual addition of wastes like toilet cleaners, caustic potash etc.. Due to sudden fall in DO less than 4 mg/l during April and May, 2014 and increment of ammonia after addition of toilet wastes in Sept.19, 2015 ; mass mortality of fishes Labeo rohita, Cirrhina mrigala, Hypothalamichthys molitrix, Aristichthysnobilis occurred in the study site.


2010 ◽  
Vol 34 (2) ◽  
pp. 142-147
Author(s):  
Fouad Kamil Salum

This study designed to know the effect of use different ration from mixing between minced meat With fat on total bacterial count of meat ( 80) sample was examine through the period of this study (40 sample for minced meat and 40 sample for minced fat) collected from shops in Baghdad city. The results showed the means of bacterial count was rise for the group contained just minced meat without any mixing in means reach to 9.6x 10 cfu \ml follow it with second group which include only minced fat by count 5.5x10 cfu\ml, and the study showed also decrease in bacterial count for (3) mixing groups ( fat with meat ), so the bacteria count for the third group which included 5%, from fat 1.1x10 cfu\ml and 1.3x10 for each of fourth and fifth group which contain the percent of fat10%,20%0n respectively. Also the study shows presence of a significant difference in ( p<0.05) between different mixing group (meat with fat) from one side to two qroups which included meat and fat only. Also recorded significant difference and in the same level in the mean of bacterial count between the first group which contain minced meat only, and the second one which contain fat only, there is no significant difference in mean of bacterial count among varying mixing group from statistical side but record obvious and important decline hygienically in bacterial count for each group. The study showed the best mixing percent for fat with minced meat were 10%,20% respectively which recorded the less bacterial count in each of these two groups. Conclusion mix fat with meat in ratios (10% - 20%) to get the beast decreas in bacterial load of meat .


Author(s):  
Swati Kanungo ◽  
Nirmal Kumar Bhuyan ◽  
Hemanta Kumar Patra

The present investigation is aimed at assessing the current water quality standard along the stretch of Brahmani river in terms of physico-chemical parameters. In the selected study area, river Brahmani is receiving a considerable amount of industrial wastes and witnessing a considerable amount of human and agricultural activities .Nine samples were collected along the entire stretches of the river basin during the period from September-2013 to August-2014 on the first working day of every month. Various physico-chemical parameters like pH,, EC, total hardness, total alkalinity sodium, potassium, calcium, magnesium, nitrate, sulphate  D.O., B.O.D. etc. were analyzed. The present study indicates that the water quality of Brahmani river is well within tolerance limit taking the physico-chemical parameters into considerations.


2016 ◽  
Vol 43 (2) ◽  
pp. 239-250
Author(s):  
Md Ashrafur Rahman ◽  
Sabira Sultana ◽  
Md Abdus Salam

Study was conducted to determine the physico-chemical parameters of water of three different lakes in Jahangirnagar University campus from March to November, 2013. The selected parameters were water depth, water temperature, conductivity, transparency, pH, dissolve O2, free CO2, total alkalinity, hardness, sulfate and water color. Water depth fluctuated from 140 cm to 385 cm, temperature ranged from 250C to 310C, conductivities ranged from 627 ?S/cm to 1544 ?S/cm, transparency from 22 cm to 87 cm, considerable variation of water colors were found and pH ranged from 6.4 to 7.5 without having any significant fluctuation. Dissolve oxygen (DO) ranged from 2.7 mg/l to 7.6 mg/l, carbon dioxide level ranged from 16 mg/l to 62 mg/l, total alkalinity and hardness were ranged from 43 mg/l to 92 mg/l and 63 mg/l to 134 mg/l, respectively with slight variations in different lakes in different months. During the study period, all the spots showed minimum fluctuation of physico-chemical parameters from the normal range without having some exceptions.Bangladesh J. Zool. 43(2): 239-250, 2015


2016 ◽  
Vol 4 (1) ◽  
pp. 4
Author(s):  
Hayat Hassan ◽  
Hatil EL-Kamali

<p>Total viable counts of bacteria and bacterial diversity of the different soil samples from three different localities in Sharq EL-Neel region: Soba, AL-Aelafoon and Um Dawan Ban sub-regions were carried out. Soil physical and chemical characteristics (pH, EC,SP,solublecations: Na, K, Ca, Mg and anion P, organic carbon, total nitrogen and soil texture)in each studied sub-regions were measured. Qualitative analysis of microorganisms isolated from the studied soil samples reveal a total of thirteendifferent species of bacteria, of which two are unidentified. The ten species are classified under Bacillus genus. In Sharq EL-Neel regionsoil samples, total bacterial counts ranged from 9.5 × 10<sup>4 </sup>cfu g<sup>-1</sup> to 1×10<sup>3</sup> with a mean of 4×10<sup>3 </sup>cfu g<sup>-1</sup>. The quantitative data on microbial population recorded in the present study was analysed using two diversity indices. High Shannon-Weiner diversity Index value for bacteria was obtained in AL-Aelfoon sub-region (1.79361), whereas high Simpson's index value was obtained in Um Dawan Ban sub-region (2.80).ActinomycesActinomyces spp. and Streptomyces spp. Where the most abundant microorganisms identified in the three sub-regions. Total bacterial count in Soba soil was positively correlated with pH (r= 0.0194) and sand (r= 0.3205); the total bacterial count in AL-Aelafoon soilwas positively correlated with EC (r= 0.1062), clay (r= 0.3816), silt (r= 0.1936), SP (r= 0.9302), K (r= 0.6252), Ca (r= 0.0015) and Mg (r= 0.1556), whereas the total bacterial count in Um Dawan Ban soil was positively correlated with clay (r= 0.2614), silt (r= 0.0216), SP (r= 0.565), K (r= 0.9645), P (r= 0.0197), Ca (r= 0.7377), Mg (r= 0.0267), N (r= 0.5215) and O.C (r= 0.3214). There were obvious differences in correlation coefficients among the selected criteria (46 % from the total number of correlation coefficients were positively correlated between bacterial counts and soil physico-chemical properties whereas 54% from the total number were positively correlated between plant type and bacterial counts).</p>


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