scholarly journals Green Synthesis and Optimization of Zinc Oxide Quantum Dots Using the Box-behnken Design, With Anticancer Activity Against the MCF-7 cell line

2021 ◽  
Author(s):  
R. Mary Nancy Flora ◽  
S. Palani ◽  
J. Sharmila ◽  
M.CHAMUNDEESWARI M
Keyword(s):  
Quantum Dots ◽  
Zinc Oxide ◽  
Anticancer Activity ◽  
Cost Effective ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Great Promise ◽  
Zno Nps ◽  
Box Behnken Design ◽  
Zno Qds

Abstract A green strategy and cost-effective approach was adapted to prepare Zinc oxide quantum dots (ZnO-QDs) for biomedical applications. The prepared ZnO-QDs may hold great promise as sensing scanners for diagnostics and therapy, as demonstrated in our current study. Zinc Sulphate, Azadirachta indica, and Catharanthus roseus leaves extract were used to synthesis a novel natural Zinc oxide bionanocomposite (ZnO-BC) and used as a precursor to prepare ZnO-QDs by microwave-assisted technique. The ZnO-BC was characterized by SEM-EDX, FTIR, XRD, Zeta potential and particle size analysis. The optical properties of QDs were investigated using UV and PL spectrophotometers. Experimental factors like the concentrations of ZnO-NPs, C. roseus and A. verawere evaluated using Box-Behnken design (BBD). MTT and haemolysis assay was performed using ZnO-BC and ZnO-QDs. Maximum absorbance observed at optimized values of 0.5% ZnO-NPs, 1g A.vera gel and 0.5ml C.roseus leaf extract of ZnO-QDs against BBD. There was decreased viability rate, ranging from 60-15% for 0.5mg/ml ZnO-BC and 45-5% for 5 mg/ml ZnO-QDs which revealed a tenfold decrease in cell viability with less concentration scale for 5mg/ml of ZnO-QDs when compared with that of 0.5 mg/ml ZnO-BC. Also, hemolysis test shows that the hemolysis ratio was below 0.5%, indicating non-haemolysis of ZnO-QDs. cellular morphology by results was supported by phase-contrast microscopy images. A good biocompatibility and high anticancer activity was noticed for ZnO-QDs when compared to ZnO-BC and provide versatile applications in the field of Nano biomedicine.

scholarly journals Nano Zinc Oxide Green-Synthesized from Plumbago auriculata Lam. Alcoholic Extract

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2447
Author(s):  
Mina Michael Melk ◽  
Seham S. El-Hawary ◽  
Farouk Rasmy Melek ◽  
Dalia Saleh ◽  
Omar M. Ali ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Particle Size ◽  
Zinc Oxide ◽  
Plant Extract ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Alcoholic Extract ◽  
Zno Nps ◽  
Subtype B ◽  
Average Size

Zinc oxide nanoparticles (ZnO NPs) were synthesized by using an alcoholic extract of the flowering aerial parts of Plumbago auriculata Lam. Dynamic Light Scattering (DLS) revealed that the average size of synthesized ZnO NPs was 10.58 ± 3.350 nm and the zeta potential was −19.6 mV. Transmission electron microscopy (TEM) revealed that the particle size was in the range from 5.08 to 6.56 nm. X-ray diffraction (XRD) analysis verified the existence of pure hexagonal shaped crystals of ZnO nanoparticles with an average size of 35.34 nm in the sample, which is similar to the particle size analysis acquired by scanning electron microscopy (SEM) (38.29 ± 6.88 nm). HPLC analysis of the phenolic ingredients present in the plant extract showed that gallic acid, chlorogenic acid, and catechin were found as major compounds at concentrations of 1720.26, 1600.42, and 840.20 µg/g, respectively. Furthermore, the inhibitory effects of ZnO NPs and the plant extract against avian metapneumovirus (aMPV) subtype B were also investigated. This assessment revealed that the uncalcinated form of Nano-ZnO mediated by P. auriculata Lam. extract possessed a significant antiviral activity with 50% cytotoxic concentration (CC50) and 50% inhibition concentration (IC50) of 52.48 ± 1.57 and 42.67 ± 4.08 µg/mL, respectively, while the inhibition percentage (IP) was 99% and the selectivity index (SI) was 1.23.

scholarly journals Novelty of Bioengineered Iron Nanoparticles in Nanocoated Surgical Cotton: A Green Chemistry

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Bhavika Turakhia ◽  
Saujanya Chikkala ◽  
Sejal Shah
Keyword(s):  
Antimicrobial Activity ◽  
Particle Size ◽  
Green Chemistry ◽  
Iron Nanoparticles ◽  
Synthesis Method ◽  
Cost Effective ◽  
Zingiber Officinale ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Ginger Extract

The current focus of nanotechnology is to develop environmentally safe methodologies for the formulation of nanoparticles. The phytochemistry of Zingiber officinale inspired us to utilize it for the synthesis of iron nanoparticles. GC-MS analysis revealed the phytochemical profile of ginger. Out of 20 different chemicals, gingerol was found to be the most potent phytochemical with a retention time of 40.48 min. The present study reports a rapid synthesis method for the formation of iron nanoparticles and its potential efficacy as an antibacterial agent and an antioxidant. Because of its antibacterial property, ginger extract was used to coat surgical cotton. Synthesized ginger root iron nanoparticles (GR-FeNPs) were characterized by UV-visible spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction analysis, and particle size analysis. XRD confirmed the crystalline structure of iron oxide nanoparticles as it showed the crystal plane (2 2 0), (3 1 1), (2 2 2), and (4 0 0). The particle size analyzer (PSA) showed the average size of the particles, 56.2 nm. The antimicrobial activity of the FeNPs was tested against different Gram-positive and Gram-negative bacteria. E. coli showed maximum inhibition as compared with the other organisms. Antioxidant activity proved the maximum rate of free radicals at 160 µg/mL produced by nanoparticles. In addition, the antimicrobial activity of nanocoated surgical cotton was evaluated on the first day and 30th day after coating, which clearly showed excellent growth inhibition of organisms, setting a new path in the field of medical microbiology. Hence, iron-nanocoated surgical cotton synthesized using green chemistry, which is antimicrobial and cost effective, might be economically helpful and provide insights to the medical field, replacing conventional wound healing treatments, for better prognosis.

ZnO quantum dots and graphene based heterostructure for excellent photoelastic and highly sensitive ultraviolet photodetector

RSC Advances ◽  
2015 ◽  
Vol 5 (110) ◽  
pp. 90838-90846 ◽  
Author(s):  
Buddha Deka Boruah ◽  
Abha Misra
Keyword(s):  
Quantum Dots ◽  
Zinc Oxide ◽  
Ultraviolet Photodetector ◽  
Highly Sensitive ◽  
Zno Quantum Dots ◽  
Ultraviolet Photodetectors ◽  
Zno Qds

Heterostructures comprised of zinc oxide quantum dots (ZnO QDs) and graphene are presented for ultraviolet photodetectors (UV PD).

scholarly journals Optimization and green synthesis of zinc oxide nanoparticle using Garcinia cambogia leaf and evaluation of their antioxidant and anticancer property in kidney cancer (A498) cell lines

Biomedicine ◽  
2021 ◽  
Vol 41 (2) ◽  
pp. 206-222
Author(s):  
Varsha Jayakar ◽  
Vinayak Lokapur ◽  
B. R. Nityasree ◽  
Raju Krishna Chalannavar ◽  
Lyned D. Lasrado ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Green Synthesis ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Zno Nps ◽  
Xtt Assay ◽  
Garcinia Cambogia ◽  
Hek 293 ◽  
A498 Cell ◽  
Hek 293 Cell Line

Introduction and Aim: In 21stcentury, nanomedicine has turned out to be an emergent modulus operation for the diagnosis and treatment of cancer. The current study includes the optimization and green synthesis of zinc oxide nanoparticles from the leaves of Garcinia cambogia and interpretation of its antioxidant and anticancer activity.   Materials and Methods: Synthesized ZnO NPs were investigated by UV–vis, FTIR, XRD, SEM, EDX, HR-TEM, SAED, particle size distribution and DLS analysis. Furthermore, different antioxidant assays were conducted for evaluating the antioxidant activity of (ZnO NPs) and its anticancer activity using (A498) cell lines along with non-cancerous (HEK-293) cell line using MTT assay and XTT assay.   Results: The UV–visible spectrum showed an absorption peak at 277 nm that reflects surface plasmon resonance (SPR) ZnONPs. The presence of various functional groups was confirmed by FTIR analysis. XRD patterns indicated that the ZnO NPs had a single-phase nature with the wurtzite structure. SEM, TEM pictures confirmed the size and shape of synthesized Nanoparticles ranges of 11–32 nm and mostly hexagonal forms. The Zeta potential value of 57.9 mV revealed the surface charge of green synthesized ZnONPs. The cytotoxicity of the ZnO NPs with various concentrations (6.25 to 200 µg/mL) was measured in (A498) cell lines along with non-cancerous (HEK-293) cell line. After the exposure, to MTT and XTT assay, cellular morphology studies showed that the cancer cell viability decreased with increasing ZnO NP concentration.   Conclusion: The synthesized ZnONPs via a green synthetic route could be developed as anti-cancer agent against cancer cells.

scholarly journals The Effects of Zinc Oxide (ZnO) Quantum Dots (QDs) Embedment on the Physicochemical Properties and Photocatalytic Activity of Titanium Dioxide (TiO2) Nanoparticles

2021 ◽  
Vol 32 (2) ◽  
pp. 71-85
Author(s):  
Anwar Iqbal ◽  
Usman Saidu ◽  
Farook Adam ◽  
Srimala Sreekantan ◽  
Normawati Jasni ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Zinc Oxide ◽  
Titanium Dioxide ◽  
Physicochemical Properties ◽  
Quantum Confinement Effect ◽  
Sol Gel ◽  
Average Crystallite Size ◽  
Fluorescent Light ◽  
Zno Quantum Dots ◽  
Zno Qds

In this study, a detailed investigation on the effect of zinc oxide (ZnO) quantum dots (QDs) embedment on the physicochemical properties of anatase titanium dioxide (TiO2) was conducted. The highly porous nanocomposite labelled as ZQT was prepared via the sol-gel assisted hydrothermal method. The powder X-ray diffraction (XRD) analysis indicates that the average crystallite size of the ZnO QDs, anatase TiO2 (TiO2 NPs) and ZQT were 4.45 nm, 9.22 nm and 11.38 nm, respectively. Photoluminescent (PL) analysis detected the presence of defects related to TiO2, oxygen vacancies and quantum confinement effect (QCE) of the ZnO QDs in ZQT. These features enhanced the photodegradation of tetracycline (TC) under 48 watt of fluorescent light irradiation when ZQT (98.0%) was used compared to TiO2NPs (32.4%) and ZnO QDs (68.8%). The photodegradation activity was driven by O2●− followed by ●OH and h+.

Precursor Concentration Effect on Physicochemical Properties of Zinc Oxide Nanoparticle Synthesized with Banana Peel Extract

2019 ◽  
Vol 797 ◽  
pp. 262-270 ◽  
Author(s):  
R.A. Abdol Aziz ◽  
Siti Fatma Abd Karim ◽  
Ummi Kalthum Ibrahim ◽  
N Sanuddin
Keyword(s):  
Zinc Oxide ◽  
Food Packaging ◽  
Cost Effective ◽  
Xrd Analysis ◽  
Band Gap Energy ◽  
Banana Peel ◽  
Zno Nps ◽  
Constant Ph ◽  
Oxide Nanoparticle ◽  
Musa Species

The present study reports the synthesis of zinc oxide (ZnO) nanoparticles (NPs) using Jackfruit banana peel (Musa Species.) extracts (BPE) as reducing and stabilizing agent. This green synthesis is considered promising an alternative technique that cost effective, nontoxic and environmental friendly. Zinc acetate dehydrate solution ((CH3COO)2.2H2O) was used as the precursor for ZnO synthesis and the concentration was varied in the range of 0.100 M – 0.010 M at constant pH of solutions, 12. The synthesized ZnO NPs were then characterized using fourier transform infrared spectroscopy (FTIR), ultraviolet visible (UV-Vis) absorption spectroscopy, x-ray Diffractometer and Brunauer-Emmett-Teller (BET). The band gap energy was found in the range of 3.44 eV - 3.58 eV while XRD analysis shows a crystalline structure in hexagonal wurtzite shape. These unique characteristics open the possibilities of various potential application in medical and industry as well as for development of antimicrobial agent for food packaging applications.

Defect-rich ZnO quantum dots as a potential multifunctional sunscreen and cosmetic active ingredient

2015 ◽  
Vol 87 (9-10) ◽  
pp. 971-977 ◽  
Author(s):  
Adersh Asok ◽  
Ajit R. Kulkarni ◽  
Mayuri N. Gandhi
Keyword(s):  
Quantum Dots ◽  
Active Ingredient ◽  
Visible Region ◽  
Band Edge ◽  
Band Edge Emission ◽  
Edge Emission ◽  
Zno Nps ◽  
Visible Luminescence ◽  
Zno Quantum Dots ◽  
Zno Qds

AbstractIn sunscreens, ZnO nanoparticles (NPs) are used as inorganic UV filter which have a prominent band edge emission in the UVA region (~385 nm). When applied to biological surface, this highly penetrating UVA emission from ZnO NPs would enhance the generation of reactive oxygen species resulting in oxidative stress. Therefore, the elimination of this harmful UVA emission from ZnO NPs are much sought after for the development of safer sunscreens. In this paper we introduce the use of defect-rich ZnO (D-ZnO) quantum dots (QDs) as a multifunctional active ingredient in sunscreen/cosmetic application. These D-ZnO QDs make use of their defect energy levels to emit in visible region by bypassing their harmful band edge emission at UVA region. The D-ZnO QDs also showed prominent visible luminescence which matches well with the autofluorescence of in vivo human skin. Hence, this visible luminescence could be useful for camouflaging, thereby enabling its potential as a biologically safe active ingredient for both cosmetic and UV screening applications.

scholarly journals Synergism of Zinc Oxide Quantum Dots with Antifungal Drugs: Potential Approach for Combination Therapy against Drug Resistant Candida albicans

2021 ◽  
Vol 3 ◽  
Author(s):  
Preeti Chand ◽  
Sangeeta Kumari ◽  
Neelima Mondal ◽  
Surinder P. Singh ◽  
Tulika Prasad
Keyword(s):  
Quantum Dots ◽  
Drug Resistance ◽  
Zinc Oxide ◽  
Candida Albicans ◽  
Combination Therapy ◽  
Antifungal Drugs ◽  
Low Concentrations ◽  
Drug Doses ◽  
Zno Qds

Candidiasis caused by Candida albicans is one of the most common microbial infections. Azoles, polyenes, allylamines, and echinocandins are classes of antifungals used for treating Candida infections. Standard drug doses often become ineffective due to the emergence of multidrug resistance (MDR). This leads to the use of higher drug doses for prolonged duration, resulting in severe toxicity (nephrotoxicity and liver damage) in humans. However, combination therapy using very low concentrations of two or more antifungal agents together, can lower such toxicity and limit evolution of drug resistance. Herein, 4–6 nm zinc oxide quantum dots (ZnO QDs) were synthesized and their in vitro antifungal activities were assessed against drug-susceptible (G1, F1, and GU4) and resistant (G5, F5, and GU5) isolates of C. albicans. In broth microdilution assay, ZnO QDs exhibited dose dependent growth inhibition between 0 – 200 µg/ml and almost 90% growth was inhibited in all Candida strains at 200 µg/ml of ZnO QDs. Synergy between ZnO QDs and antifungal drugs at sub-inhibitory concentrations of each was assessed by checkerboard analysis and expressed in terms of the fractional inhibitory concentration (FIC) index. ZnO QDs were used with two different classes of antifungals (azoles and polyenes) against Candida isolates: combination 1 (with fluconazole); combination 2 (with ketoconazole); combination 3 (with amphotericin B), and combination 4 (with nystatin). Results demonstrated that the potency of combinations of ZnO QDs with antifungal drugs even at very low concentrations of each was higher than their individual activities against the fungal isolates. The FIC index was found to be less than 0.5 for all combinations in the checkerboard assay, which confirmed synergism between sub-inhibitory concentrations of ZnO QDs (25 µg/ml) and individual antifungal drugs. Synergism was further confirmed by spot assay where cell viabilities of Candida strains were significantly reduced in all combinations, which was clearly evident from the disappearance of fungal cells on agar plates containing antifungal combinations. For safer clinical use, the in vitro cytotoxic activity of ZnO QDs was assessed against HeLa cell line and it was found that ZnO QDs were non-toxic at 25 µg/ml. Results suggested that the combination of ZnO QDs with drugs potentiate antimicrobial activity through multitargeted action. ZnO QDs could therefore offer a versatile alternative in combination therapy against MDR fungal pathogens, wherein lowering drug concentrations could reduce toxicity and their multitargeted action could limit evolution of fungal drug resistance.

scholarly journals EFFECT OF CE3+ METAL IONS ON THE ANTIBACTERIAL AND ANTICANCER ACTIVITY OF ZINC OXIDE NANOPARTICLES PREPARED BY COPRECIPITATION METHOD

2017 ◽  
Vol 10 (3) ◽  
pp. 388 ◽  
Author(s):  
Theivarasu C ◽  
Indumathi Thangavelu
Keyword(s):  
Zinc Oxide ◽  
Anticancer Activity ◽  
Zinc Oxide Nanoparticles ◽  
Human Lung ◽  
Human Lung Cancer ◽  
Oxide Nanoparticles ◽  
Lung Cancer Cell ◽  
Zno Nps ◽  
Doped Zno ◽  
Antibacterial And Anticancer Activity

ABSTRACTObjective: This study was undertaken to know about the antibacterial and anticancer activity of synthesized zinc oxide (ZnO) nanoparticles (NPs).Methods: The ZnO NPs and different concentration of Ce3+ (0.05M, 0.10M, and 0.15M)-doped ZnO NPs were synthesized by coprecipitation method.The synthesized nanoparticles were analyzed by X-ray diffraction (XRD) and HRSEM. The antibacterial studies were performed against a set ofbacterial strains as Gram-positive bacteria (Streptococcus aureus and Streptococcus pneumonia) and Gram-negative (Escherichia coli, Pseudomonasaeruginosa, Proteus vulgaris, Klebsiella pneumonia, and Shigella dysenteriae) bacteria. The cytotoxic effect of ZnO and Ce-doped ZnO was analyzed incultured (A549) human lung cancer cell line.Result: The XRD studies showed the wurtzite structure of nanoparticles. HRSEM analysis showed the spherical shape of ZnO and Ce-doped ZnO. TheZn0.85Ce0.15O NPs possessed more antibacterial effect as compared to the other ZnO and Ce-doped ZnO NPs. The Zn0.90Ce0.10O NPs created the highestcytotoxicity activity. With respect to cell death, as low a concentration of 68±0.05 μg/ml of Zn0.90Ce0.10O NPs was good enough to cause loss of viabilityof 50% of the cell as compared to ZnO and Zn1-xCexO (x=0.05 and 0.15) NPs.Conclusion: Results from this work concluded that Zn0.85Ce0.15O and Zn0.90Ce0.10O NPs possess antibacterial and anticancer activity, respectively.Keywords: Zinc oxide nanoparticles, Coprecipitation method, Antibacterial activity and anticancer activity, Human lung cancer cell line.

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