scholarly journals Animal Study of a Newly Designed Metal Airway Brachytherapy Stent Loaded with Radioactive 125I Seeds

2021 ◽  
Author(s):  
Dechao Jiao ◽  
Qinyu Lei ◽  
Kaihao Xu ◽  
Yiming Liu ◽  
Xinwei Han
Keyword(s):  
Irradiation Dose ◽  
White Blood Cells ◽  
Injury Score ◽  
Test Items ◽  
Dose Accumulation ◽  
125I Seeds ◽  
Airway Stenting ◽  
Tissue Changes ◽  
Histopathologic Analysis ◽  
Group B

Abstract Aim: To evaluate dynamic tissue changes after airway stenting (AS) with a newly designed metal brachytherapy stent (BS) loaded with radioactive 125I seeds in normal rabbits.Method: Forty-five normal New Zealand white rabbits were divided into three groups (Group A: stent without seeds; Group B: stent with 0.4 mCi active seeds; Group C: stent with 0.8 mCi active seeds) and underwent AS under C-arm guidance. Then, 5 rabbits were sacrificed from each group at 2, 4, and 8 weeks for further examination. Laboratory tests (including routine blood tests, liver function, kidney function, electrolytes and ROS levels), gross observations, and tissue changes of Masson/hematoxylin-eosin staining, plus immunohistochemistry of α-SMA, NOX4, and TGF-β were performed at each time point.Result: All animals underwent AS successfully without procedure-related death, but one animal died at 6 weeks due to severe pulmonary infection in Group C. Apart from a transient increase in white blood cells (P < 0.05) and a gradual increase in ROS levels (P < 0.05), other blood test items showed no significant changes (P > 0.05). The brachytherapy injury score increased with irradiation dose accumulation (P < 0.05), but tissue hyperplasia at the stent end in Group C was less severe than that in Groups A and B (P < 0.05). Airway lateral fibrosis was observed in all groups by histopathologic analysis; however, fibrosis in Group C was more severe than that in Groups A and B (P < 0.05).Conclusion: The brachytherapy injury score increased with irradiation dose accumulation, while granulation tissue hyperplasia at the stent end was inhibited by 125I brachytherapy within 8 weeks.

scholarly journals BIOBRAN MGN-3

2012 ◽  
Vol 20 (01) ◽  
pp. 13-16
Author(s):  
Ahmad Ijaz Masood ◽  
RABEETA SHEIKH ◽  
RANA ATIQUE ANWER
Keyword(s):  
Quality Of Life ◽  
Side Effects ◽  
Hair Loss ◽  
White Blood Cells ◽  
Food Supplement ◽  
Severe Nausea ◽  
Treatment Side Effects ◽  
Group A ◽  
Group B

Objective: The aim of study was to assess the effect of Biobran in reducing of chemotherapy induced side effects in termsof tiredness, anorexia, vomiting and hair loss and quality of life in terms of weight loss. Setting: Radiotherapy Department, NishtarHospital Multan. Material and Methods: Fifty patients of breast cancer were enrolled randomly in two groups. Group-A patients weregiven 3 gram dose of Biobran MGN-3 per day one week before and one week after chemotherapy. Group-B patient were givenchemotherapy alone. Total six cycles of chemotherapy were given. No multivitamin or food supplements were given during this study.Chemotherapy induced side effects (tiredness, anorexia, and vomiting, hair loss) were assessed by questionnaire to the patients beforestart of each cycle. Weight was checked before each cycle to assess weight gain or loss. White blood cells were checked by completeblood count just before and one week after chemotherapy. Results: Between six months, 50 patients were enrolled in RadiotherapyDepartment, Nishtar Hospital Multan. There was a significant reduction in tiredness and anorexia in group-A patients. 20 (80%) patients ofgroup-A felt increase in their diet and no tiredness without any appetizer or multivitamin. But group-B patients demanded for appetizer dueto severe anorexia after chemotherapy except 3 (12%) patients who didn’t use any appetizer or food supplement. In group-A, 15 (60%)patients didn’t need any anti-emetic as compared to group-B all patient (100%) experienced severe nausea during and afterchemotherapy. Group-A patients experienced less hair fall 7 (28%) patients as compared to other group which is 25 (100%) patients.Conclusions: The study showed that, by helping to optimize the immune system, Biobran MGN-3 can not only help maximize treatmentsuccess, but also minimize treatment side effects and improve quality of life during treatment and in recovery.

Antidiabetic activity of aqueous extract of leaves of cnestis ferruginea vahl ex DC. (Connaraceae) on alloxan-diabetic induced mice.

2021 ◽  
Vol 4 (1) ◽  
pp. 1-6
Author(s):  
Emile K. Begbin ◽  
Edwige A. Odoh ◽  
Ernest N. Zougrou ◽  
Landry Claude A. Kablan ◽  
Diéneba Kone-Bamba ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Aqueous Extract ◽  
White Blood Cells ◽  
Hdl Cholesterol ◽  
Treated Group ◽  
Antidiabetic Activity ◽  
Metformin Group ◽  
Hematological And Biochemical Parameters ◽  
Group B

Abstract Background: Cnestis ferruginea is a plant species widely used in the traditional african medicine to treat diabetes mellitus. The present study aimed to evaluate the antidiabetic activities of the aqueous extract of the leaves of Cnestis ferruginea (AECF) in diabetic mice. Methods: The mice were grouped into five groups of five animals per group: Group A was not induced with alloxan, Group B animals were induced but not treated, Group C animals were treated with 500 mg / kg of BW of metformin, Group D and E animals were treated with 100, 200 mg/kg BW of AECF respectively. The extracts were administered to the animals orally for 14 days. Fasting blood sugar was measured by a glucometer. Serum concentrations of hematological and biochemical parameters were measured by standard methods. Results: The animals administered with 100 and 200 mg/Kg B.W of extract showed highly significant decrease (P ˂ 0.0001) in blood sugar level compared to the untreated animals. The 100 mg / kg BW dose of AECF produce low significant decrease (P <0.05) of total cholesterol, HDL Cholesterol, AST, ALT, urea, levels of white blood cells, platelets, hematocrit. The 200 mg / kg BW dose of AECF produce in addition significant decrease (P <0.01) of AST and urea. Conclusion: The 200 mg / kg dose of BW of AECF had a greater antidiabetic activity than the dose of 100 mg / kg BW. Keywords: Cnestis ferruginea; antidiabetic activity; alloxan; diabetes mellitus.

scholarly journals The Effect of a Mediterranean Meal on Sprague Dawley Rats DMBA-Induced Mammary Tumors

2010 ◽  
Vol 3 ◽  
pp. CGM.S5894
Author(s):  
Paula C. Pereira ◽  
A. Filipa Vicente ◽  
Maria F. Mesquita ◽  
Antonio S. Cabrita
Keyword(s):  
Experimental Studies ◽  
Protective Role ◽  
Female Rats ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Total Cancer ◽  
Group A ◽  
Histopathologic Analysis ◽  
Group B

The present study intents to find a possible protective role of a Mediterranean type meal on mammary carcinogenesis. Several factors have been associated with breast cancer risk, genetics and environment are the most pointed out in epidemiologic and experimental studies. Diet is an environmental factor that can promote or prevent disease, being responsible for almost 35% of total cancer cases. A total of 72 female rats 50 days old were randomly divided in three groups of 24 rats and housed 4 in each plastic cage in a holding room under constant conditions of 22 ± 2 °C, 55 ± 10% humidity and a 12 h light/dark cycle. All the animals were submitted to the administration of 20 mg of 7, 12 dimethylbenzanthracene (DMBA) in olive oil, by gavages, except group A. The same defined standard food was provided for all the animals in group A and B, supplemented with a Mediterranean meal in group C. All the animals were sacrificed by the end of 150 days. Total carcinoma number did not differ significantly between Groups B and C and there were not found any neoplastic lesions in Group A. Most tumors showed a mixed architectural pattern, with cribriform and papillary areas, comedocarcinoma and necrosis was only seen in Group B. Histopathologic analysis showed that Group C tumors had lower mitotic activity and Pattern Grades, but higher Nuclear Grades. Mediterranean diet type meal showed lower Pattern Grades and lower Mitotic count in spite of that a higher nuclear pleomorphism was also found. Even so, tumors from Group C were better differentiated which can indicate lower malignancy.

The approximate formulas predicting personal somatic telomere length using patient blood test data

2019 ◽  
Vol 97 (11) ◽  
pp. 1090-1093
Author(s):  
Toyoki Maeda ◽  
Takahiko Horiuchi ◽  
Naoki Makino
Keyword(s):  
Telomere Length ◽  
Test Data ◽  
Blood Cells ◽  
Blood Test ◽  
White Blood Cells ◽  
Laboratory Data ◽  
Biological Aging ◽  
Differential Prediction ◽  
Test Items ◽  
Peripheral Leukocytes

Biological aging underlies lifestyle-related diseases. It can be assessed by measuring personal somatic cell telomere length. However, measuring the telomere length is laborious, and its clinical surrogate parameters have not been developed. This study analyzed the correlation between telomere length in peripheral leukocytes and laboratory data to select test items relating closely to biological aging. We established formulas from these clinical data to predict the personal telomere length. The subjects were patients having visited Kyushu University Beppu Hospital from 2012 to 2015. Two hundred and thirty-two patients were enrolled. The blood data were collected and telomere lengths were measured by Southern blotting method. The patients showed significant correlations between the telomere length and several blood test data with a sex-related difference. Candidate formulas are as follows: Predicted telomere length (kb) in men = 8.59 − 0.037 × Age (years) + 0.024 × Hemoglobin (g/dL); Predicted telomere length (kb) in women = 4.83 − 0.019 × Age (years) + 0.23 × Albumin (g/dL) + 0.0001 × White blood cells (/mm3) + 0.0020 × Red blood cells (× 104/mm3) + 0.0032 × Total cholesterol (mg/dL). Thus, the derived formulas allow for the accurate differential prediction of telomeric length in male and female patients.

scholarly journals The use of RemoweLL oxygenator-integrated device in the prevention of the complications related to aortic valve surgery in the elderly patient: Preliminary results

2018 ◽  
Vol 25 (1_suppl) ◽  
pp. 59-65
Author(s):  
Alberto Molardi ◽  
Maria V Di Chicco ◽  
Davide Carino ◽  
Matteo Goldoni ◽  
Matteo Ricci ◽  
...  
Keyword(s):  
Cardiopulmonary Bypass ◽  
Aortic Valve ◽  
Length Of Stay ◽  
Elderly Patients ◽  
Interleukin 6 ◽  
Blood Cells ◽  
White Blood Cells ◽  
Lower Amount ◽  
Parametric Data ◽  
Group B

Background The effects of fat microembolization due to cardiopulmonary bypass are well known in cardiac surgery. Our aim is to evaluate the use of the RemoweLL device (Eurosets, Medolla, Italy) during elective aortic valve replacement in elderly patients (>70 years old) to rate its biochemical and clinical effects. The RemoweLL device is an oxygenator-integrated reservoir which combines two strategies for fat emboli and leucocytes removal: filtration and supernatant elimination. Methods Forty-four elderly patients were enrolled and assigned randomly to a Group A (standard device) and a Group B (RemoweLL). Biochemical effects were evaluated by blood samples, which were tested for white blood cells, neutrophils, protein SP-100 and interleukin 6 besides standard lab tests. Our clinical endpoints were any type of neurological, cardiac, respiratory, gastrointestinal or renal complications, and length of stay in the intensive care unit. Statistical analysis was carried out with chi square test for non-parametric data; t test and analysis of variance for repeated measures were used for parametric data. Results Group B showed lower levels of white blood cells, neutrophils, interleukin 6 and protein SP-100 immediately and 24 hours after the operation. Group B also showed a lower amount of neurocognitive type II dysfunction even if the length of stay in the ICU did not change. Conclusions The RemoweLL system is safe and effective in reducing inflammatory response to cardiopulmonary bypass and it could be a useful tool in minimizing negative effects of cardiopulmonary bypass; however, it does not seem to have any effect on elderly patients’ hospital stay.

scholarly journals Effects of Siam Weed (Chromolaena odorata) Leaf Extract on Crude Oil-induced Toxicity

2020 ◽  
Vol 27 (3) ◽  
pp. E202031
Author(s):  
Fidelis Ifeakachuku Achuba ◽  
Charles Offor
Keyword(s):  
Crude Oil ◽  
Wistar Rats ◽  
Aqueous Ethanol ◽  
Animal Feed ◽  
White Blood Cells ◽  
Oxidative Enzymes ◽  
Chromolaena Odorata ◽  
Crude Petroleum ◽  
Male Wistar Rats ◽  
Group B

The objective of this study was to investigate the effects of the different extracts  (aqueous, ethanol and blended mixture) of Chromolaena odorata leaves on Wistar rats feed with crude petroleum (CP) tainted feed (4 ml/100 g diet). Materials and methods: Twenty-five male Wistar rats weighing 140 -180 g were used for this study. The rats were kept one in a cage. Group A which was the control was allowed diet free of crude oil and were not treated with any of the extracts. Group B were exposed to a diet contaminated with crude oil that contained 4ml of crude oil per 100 gram of animal feed without treatment. Groups C-E were fed with diets contaminated with 4ml of crude oil per 100g of feed and treated with 500mg/Kg-1 bodyweight of aqueous, ethanol and blended mixture of ethanol and aqueous extracts, respectively. The respective extracts and blended mixture were given daily to the rats via oral gavage in line with body weights. The study was for duration of twenty eight days after which the rats were sacrificed under sedation with chloroform. This was followed with collection of blood samples and organs of interest. All samples were stored at 40C and used within forty eight hour for various biochemical analyses. Results: There were significant decrease in haematological indices, liver proteins, oxidative enzymes,  and antioxidant enzymes when compare with  Group 1. Also, liver function maker enzymes, white blood cells (WBC), and malondialdehyde (MDA)   were significantly increased  by petroleum intoxication. Significant increase were observed in Hb, PCV, RBC, SO, MO, XO, SOD, CAT, albumin, total protein in the serum, liver and kidney; as against decreases in WBC, AST, ALT, ALP and MDA  in rats fed crude petroleum (CP) tainted feed that was treated with C.  odorata extract.  Conclusion: This study showed that administration of C. odorata leaves extracts to rats prevented health risk associated with crude petroleum toxicity.

scholarly journals Effect of Fluticasone and Salmeterol on Tracheal Responsiveness to Ovalbumin and Lung Inflammation, Administrated during and after Sensitization

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Zahra Gholamnezhad ◽  
Mohammad Hossain Boskabady ◽  
Mohammad Reza Khazdair ◽  
Mahmoud Hosseini ◽  
Mahdi Abbasnejad
Keyword(s):  
Fluticasone Propionate ◽  
Blood Cell ◽  
White Blood Cell ◽  
Blood Cells ◽  
Lung Inflammation ◽  
White Blood Cells ◽  
Control Group ◽  
Treatment Groups ◽  
Group B ◽  
Differential White Blood Cell

The effect of duration of administration of fluticasone propionate and salmeterol on tracheal responsiveness to ovalbumin and total and differential white blood cell in sensitized guinea pig was examined. Six groups of guinea pigs (n=7) were sensitized to ovalbumin. Three groups of them were subjected to inhaled fluticasone propionate and salmeterol, one group during sensitization (A), one group after that (for 18 days, B), and the other one during sensitization but with 18 days delay before measurements (C). Three other groups were treated with placebo in the same manner. The tracheal responsiveness to ovalbumin and total and differential white blood cells of three placebo groups were significantly higher than those of control group (P<0.001for all cases). Tracheal responsiveness to ovalbumin and total and differential white blood cell in treated groups with fluticasone propionate and salmeterol were significantly decreased compared to those of placebo groups (nonsignificant toP<0.001). The improvement in all variables in treatment groups A and C were more pronounced than group B. The results showed that fluticasone propionate and salmeterol had a prevention effect on tracheal hyperresponsiveness to ovalbumin and lung inflammation which was more pronounced when administered during than after sensitization.

scholarly journals Comparative Advantage of Keyhole Right Flank Laparotomy and Ventral Midline Celiotomy for Ovariohysterectomy in Bitches

2016 ◽  
Vol 4 (2) ◽  
pp. 198-202 ◽  
Author(s):  
Mohan Acharya ◽  
Manoj Kumar Sah ◽  
Dinesh Kumar Singh ◽  
Subir Singh ◽  
Santosh Dhakal
Keyword(s):  
Wound Healing ◽  
Packed Cell Volume ◽  
Blood Cells ◽  
White Blood Cells ◽  
Surgical Techniques ◽  
Relative Advantage ◽  
Ventral Midline ◽  
Group A ◽  
Group B ◽  
Time Required

The objective was to compare the relative advantage of adopting two ovariohysterectomy techniques: keyhole right flank laparotomy or ventral midline celiotomy, for ovariohysterectomy in bitches. Clinically healthy non pregnant bitches (n = 20; 2 ± 0.5 year age; 25 ± 5 kg weight) were used for the study. Bitches were randomly divided into two groups: group A (n = 10) bitches were ovariohysterectomized adopting keyhole right flank laparotomy, whereas in group B (n = 10) bitches were ovariohysterectomized adopting ventral midline celiotomy. Time required for completion of surgery, and length of suturing materials required was recorded, blood hematology and fibrinogen levels were analyzed, and post-operative wound healing was monitored. Length of chromic cat gut No. 1-0 (Ethicon; P < 0.01), and vicryl No. 3-0 (Ethicon; P < 0.001) used was more in ventral midline celiotomy compared with keyhole right flank laparotomy; however, similar length of chromic cat gut No. 2-0 (Ethicon; P = 0.47) was used in both the surgical techniques. Longer time (P < 0.001) was required to complete surgical procedure following ventral midline celiotomy compared with keyhole right flank laparotomy. Blood packed cell volume (PCV), total white blood cells (WBC), neutrophils, monocytes, and fibrinogen levels were statistically similar between the two surgical techniques. Wound healing was better in keyhole right flank laparotomy compared with ventral midline celiotomy.  Conclusion: Keyhole right flank laparotomy required less time for surgery, less suturing materials and better wound healing compared with ventral midline celiotomy for ovariohysterectomy in bitches. Int J Appl Sci Biotechnol, Vol 4(2): 198-202 

scholarly journals The influence of acute handling stress on some blood parameters in cultured sea bream (Sparus aurata Linnaeus, 1758)

2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Francesco Fazio ◽  
Vincenzo Ferrantelli ◽  
Gianluca Fortino ◽  
Francesca Arfuso ◽  
Giuseppe Giangrosso ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Cells ◽  
Sparus Aurata ◽  
White Blood Cells ◽  
Sea Bream ◽  
Biometric Data ◽  
Handling Stress ◽  
Haematological Profile ◽  
Group A ◽  
Group B

The effect of acute handling stress on haematological profile, blood glucose and lactate (secondary stress markers) in cultured sea bream <em>Sparus aurata</em> was evaluated. Sixty six <em>Sparus aurata</em> were used and equally divided into two groups (A and B). Group A was not subjected to stress, Group B was subjected to acute handling stress. From each fish, biometric data and blood samples were collected to evaluate haematological profile, blood glucose and lactate. Unpaired t-test Student was applied to evaluate possible differences in parameters between the two groups. Red blood cells, haematocrit, haemoglobin, white blood cells (WBC), glucose and lactate showed an increase (P&lt;0.05) in Group B compared with Group A, while mean corpuscular volume decreased (P&lt;0.05) in Group B. The results highlight the role of studied parameters in monitoring the stressful conditions of aquaculture production which affect animal welfare and fish products quality.

scholarly journals Immune status in sarcoidosis: one size does not fit all

2020 ◽  
Vol 90 (3) ◽  
Author(s):  
Ilias Papanikolaou ◽  
Hidenobu Shigemitsu ◽  
Andreas Afthinos
Keyword(s):  
Immune Status ◽  
White Blood Cells ◽  
Inverse Correlation ◽  
Retrospective Chart Review ◽  
Cell Immunity ◽  
Caucasian Origin ◽  
Group A ◽  
Group B ◽  
Serological Data ◽  
Group D

B-cell immunity and immunoglobulins are less commonly affected in sarcoidosis. We aimed to evaluate immune status in sarcoidosis patients. Retrospective chart review of sarcoidosis patients attending a out-patient clinic over 3 months period. Immunoglobulins levels were recorded (A, M, G, E) along with clinical and serological data. They were divided in group A (normal IgG), group B (increased IgG), group C (decreased IgG) and group D (decreased IgG and IgM and/or IgA). Of 50 subjects, 68% were females and 62% of Caucasian origin. 22 (44%) had normal IgG levels, 16 (32%) had increased IgG levels, 10 (20%) had hypogammaglobulinemia and 2 (4%) had combined hypogammaglobulinemia, diagnosed with combined sarcoidosis and common variable immunodeficiency. Decreased IgA values was found in groups C and D. IgE was high in group B. Globulin was increased in group B and decreased in groups C and D. Decreased neutrophils were found in group D (all statistically significant). Correlation analysis showed significant association of angiotensin converting enzyme with IgA and IgM, inverse correlation of IgG with white blood cells and neutrophils, of IgA with globulin and inverse with albumin and of calcium with albumin. Most sarcoidosis patients have normal or increased immunoglobulin levels, that correlate with serum biomarkers of disease activity. Hypogammaglobulinemia may reflect treatment side effects and accompanied by blood leukocytosis. Combined severe immunodeficiency occurs in sarcoidosis.

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