Circulating Lymphocyte Subsets are Prognostic Factors in Patients with Nasopharyngeal Carcinoma

2022 ◽  
Author(s):  
Jing Zhu ◽  
Ruhua Fang ◽  
Zhiwen Pan ◽  
Xu Qian
Keyword(s):  
Survival Rate ◽  
Nasopharyngeal Carcinoma ◽  
Mononuclear Cells ◽  
Lymphocyte Subsets ◽  
Distant Metastases ◽  
Clinical Parameters ◽  
Peripheral Blood Mononuclear ◽  
Younger Age ◽  
Ebv Dna ◽  
First Diagnosis

Abstract Background: Nasopharyngeal carcinoma (NPC) is a geographically and racially variable disease which has a high incidence in Southeast China. According to previous studies on tumor immunity, we compared multiple clinical parameters and blood indexes to find its relationship with prognosis in NPC patients.Methods: According to the load of EBV at diagnosis, 220 NPC patients receiving concurrent chemoradiotherapy (CRT) were divided into two groups. We compared clinical parameters, peripheral blood mononuclear cells, lymphocyte subsets and biochemical indexes between them. We analyzed distant metastases and overall survival rate between them.Results: In most cases, the two groups showed the same trend. Most blood indexes were decreased during CRT, the decrease in absolute count was more significant than in percentage. The younger age showed the higher CD3+ and CD3+CD8+ percentage. Patients whose EBV DNA≥1500 copies/mL at first diagnosis showed higher pN grade. Among them, higher CD3+CD8+ percentage or lower CD3-CD56+ percentage had batter OS rates. Patients whose EBV DNA<1500 copies/mL at first diagnosis had higher survival rate and longer survival time.Conclusions: CRT cause overall decrease of blood cells in NPC patients. The initial EBV load was related to prognosis. Among all the blood indexes, CD3+CD8+ percentage showed correlation with age and OS rates in patients whose EBV DNA≥1500 copies/mL at first diagnosis, which is worthy of further study.

Cytokine expression in dogs with naturalLeishmania infantuminfection

Parasitology ◽  
2009 ◽  
Vol 136 (8) ◽  
pp. 823-831 ◽  
Author(s):  
M. A. PANARO ◽  
O. BRANDONISIO ◽  
A. CIANCIULLI ◽  
P. CAVALLO ◽  
V. LACASELLA ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Clinical Status ◽  
Clinical Signs ◽  
Cytokine Expression ◽  
Tnf Alpha ◽  
Mrna Levels ◽  
Peripheral Blood Mononuclear ◽  
Ifn Gamma ◽  
First Diagnosis

SUMMARYThe aim of this study was to evaluate cytokine expression in 22Leishmania infantumnaturally infected dogs, in order to correlate this parameter with the clinical status of infected animals. After 4 and 8 months from the first diagnosis ofLeishmaniainfection, clinical and laboratory examination of dogs was performed and peripheral blood mononuclear cells (PBMC) were isolated. The cytokine profile was analysed in terms of IFN-gamma, IL-4, IL-10 and TNF-alpha mRNA expression in cultured PBMC by a semi-quantitative reverse transcriptase-PCR. Thirteen out of 22Leishmania-infected dogs remained asymptomatic in the follow-up, while 9 showed clinical signs of leishmaniasis. IL-4, IL-10, TNF-alpha and IFN-gamma mRNA levels were not significantly different in asymptomatic compared to symptomatic animals 4 months from the diagnosis ofLeishmaniainfection, but were significantly higher in symptomaticversusasymptomatic dogs after 8 months from diagnosis. In addition, IL-4, IL-10 and TNF-alpha mRNA levels significantly increased only in symptomatic dogs at 8 months, in comparison to the levels found at 4 months. These results show a mixed Th1 and Th2 cytokine response inLeishmania-infected dogs, with higher cytokine expression in dogs with manifest clinical disease, during the second follow-up after 8 months from the first diagnosis of infection.

scholarly journals Clinical relevance of virological verification methods for the etiology of infectious mononucleosis

2020 ◽  
Vol 19 (2) ◽  
pp. 29-37
Author(s):  
O. I. Demina ◽  
D. S. Tikhomirov ◽  
T. A. Chebotareva ◽  
L. N. Mazankova ◽  
T. A. Tupoleva
Keyword(s):  
Infectious Mononucleosis ◽  
Mononuclear Cells ◽  
Test Results ◽  
Elisa Method ◽  
Viral Dna ◽  
Peripheral Blood Mononuclear ◽  
Ebv Infection ◽  
Verification Methods ◽  
Ebv Dna ◽  
Mutually Independent

Purpose: to justify the need to use at least two methods (direct and indirect) for reliable laboratory decoding of infectious mononucleosis. Materials and methods. We observed 107 children with infectious mononucleosis. Deciphering the etiology was carried out using ELISA (We determined IgM VCA-EBV, IgG EA-EBV, IgG EBNA-EBV, IgM CMV, IgG CMV in serum) and PCR (We determined investigated viral DNA (EBV, CMV, HHV 6) in peripheral blood mononuclear cells). Results: In the structure of infectious mononucleosis, EBV remains the leading infection: 82 children (76.6%). In case of reactivated EBV infection, the isolated use of the ELISA method does not limit the possibility of interpreting the results without additional evaluation of the test results by PCR. A significantly level of viral DNA concentration in the examined children has not been established. The detection frequencies of EBV DNA and HHV 6 DNA by PCR are not mutually independent (p < 0.001). Detection of one of the viruses reduces the chance of detecting another virus (OR = 0.133; 95% CI from 0.0537 to 0.3273, p < 0.0001).

scholarly journals In Vitro and In Vivo Intracellular Killing Effects of Tigecycline against Clinical Nontyphoid Salmonella Isolates Using Ceftriaxone as a Comparator

2011 ◽  
Vol 55 (6) ◽  
pp. 2755-2759 ◽  
Author(s):  
Hung-Jen Tang ◽  
Wen-Chien Ko ◽  
Chi-Chung Chen ◽  
Po-Lin Chen ◽  
Han Siong Toh ◽  
...  
Keyword(s):  
Survival Rate ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Pathogen Resistance ◽  
Peripheral Blood Mononuclear ◽  
Content Type ◽  
Time Kill ◽  
Peritonitis Model

ABSTRACTSalmonellais an important, worldwide food-borne pathogen. Resistance to fluoroquinolones and cephalosporins has been increasingly reported, and new therapeutic agents are desperately needed. In this study, we evaluated thein vitroantimicrobial susceptibility of clinical nontyphoidalSalmonellaisolates to tigecycline. Antibacterial activity of tigecycline, ceftriaxone, and ciprofloxacin were investigated by time-kill studies and the murine peritonitis model. The MIC50/MIC90values of tigecycline, ceftriaxone, and ciprofloxacin against 76Salmonellaisolates were 0.25/0.5, 1/8, and 0.125/0.5 μg/ml, respectively. The intracellular inhibitory activity of tigecycline at 0.5 μg/ml (1× MIC) againstSalmonellaisolates in human peripheral blood mononuclear cells was sustained for 24 h. In a mouse peritonitis model, tigecycline reduced the extracellular and intracellular bacterial counts from 107CFU/ml and 105CFU/ml, respectively, to an undetectable level within 96 h. The results were similar to those obtained with ceftriaxone. The survival rate of mice exposed to tigecycline after being infected by an inoculum of 1 × 105CFU was 80%, and that of mice exposed to ceftriaxone was 100%. When the inoculum was increased to 1.3 × 106CFU, the survival rate of mice treated by tigecycline was 20%, and that of mice exposed to ceftriaxone was 0% (P= 0.2). When a ceftriaxone- and ciprofloxacin-resistant but tigecycline-susceptible isolate was tested, mice treated by tigecycline had a higher survival rate than those treated by ceftriaxone (15/20 [75%] versus 6/20 [30%];P= 0.011). Our results suggest that tigecycline is at least as effective as ceftriaxone for murineSalmonellainfections and warrants further clinical investigations to delineate its potential against humanSalmonellainfections.

The rational specimen for the quantitative detection of Epstein-Barr virus DNA load

2019 ◽  
Vol 57 (5) ◽  
pp. 759-765 ◽  
Author(s):  
Wang Kedi ◽  
Xu Dongjiang ◽  
Lv Zhi ◽  
Gao Yan ◽  
Jia Kun ◽  
...  
Keyword(s):  
Viral Load ◽  
Epstein Barr Virus ◽  
Mononuclear Cells ◽  
Quantitative Polymerase Chain Reaction ◽  
Diagnostic Value ◽  
Quantitative Detection ◽  
Peripheral Blood Mononuclear ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr

Abstract Background Epstein-Barr virus (EBV) DNA load monitoring in blood is essential for the diagnosis of EBV-associated diseases. However, the best-suited blood compartment for detection is still under discussion. The aim of this study was to evaluate the diagnostic value of EBV-DNA load in peripheral blood mononuclear cells (PBMC), plasma and whole blood (WB) samples. Methods A total of 156 patients, including 45 patients with infectious mononucleosis (IM), 57 patients with EBV-associated hemophagocytic lymphohistiocytosis (HLH) and 54 patients with post-transplant lymphoproliferative disorders (PTLD), were enrolled in this study. The EBV-DNA load in PBMC, plasma and WB samples were measured with real-time quantitative polymerase chain reaction (PCR). Results EBV-DNA load of patients with HLH showed no statistical difference in PBMC, plasma and WB samples, while patients with IM and PTLD showed a higher viral load in PBMC samples. The strongest correlation of EBV-DNA level was found between PBMC and WB samples among patients with IM, HLH and PTLD. The follow-up of EBV-DNA showed that the viral load became negative along with the recovery from the disease, while that in WB and PBMC would remain positive for a long time. Conclusions For the diagnosis and monitoring of EBV-DNA, the type of specimen should be chosen reasonably according to the disease. As for IM and HLH, plasma is recommended to quantify the EBV-DNA load, while PBMC and plasma are preferred in PTLD.

scholarly journals The clinical significance of EBV DNA in the plasma and peripheral blood mononuclear cells of patients with or without EBV diseases

Blood ◽  
2016 ◽  
Vol 127 (16) ◽  
pp. 2007-2017 ◽  
Author(s):  
Jennifer A. Kanakry ◽  
Aparna M. Hegde ◽  
Christine M. Durand ◽  
Allan B. Massie ◽  
Amy E. Greer ◽  
...  
Keyword(s):  
Clinical Significance ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Key Points ◽  
Blood Mononuclear Cells ◽  
Ebv Dna ◽  
Free Plasma ◽  
Better Than

Key PointsCell-free (plasma) EBV DNA performs better than cellular EBV DNA as a marker of a broad range of EBV+ diseases. Within a largely immunocompromised and hospitalized cohort, detection of EBV DNA in plasma is uncommon in the absence of EBV+ disease.

Prompt versus preemptive intervention for EBV lymphoproliferative disease

Blood ◽  
2004 ◽  
Vol 103 (10) ◽  
pp. 3979-3981 ◽  
Author(s):  
Hans-Joachim Wagner ◽  
Yee Chung Cheng ◽  
M. Helen Huls ◽  
Adrian P. Gee ◽  
Ingrid Kuehnle ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Cytotoxic T Cells ◽  
Quantitative Polymerase Chain Reaction ◽  
Lymphoproliferative Disease ◽  
Pcr Analysis ◽  
Peripheral Blood Mononuclear ◽  
Hematopoietic Stem ◽  
Preemptive Treatment ◽  
Ebv Dna

Abstract Posttransplantation lymphoproliferative disorders (PTLDs) caused by uncontrolled expansion of Epstein-Barr virus (EBV)–infected B cells after hematopoietic stem cell transplantation (HSCT) can be predicted by an increase in EBV DNA in peripheral blood mononuclear cells. We used real-time quantitative polymerase chain reaction (RQ-PCR) analysis to determine whether frequent monitoring of EBV DNA to allow preemptive treatment is truly of value in patients after HSCT. More than 1300 samples from 85 recipients were analyzed. No patient with consistently low EBV DNA levels developed PTLD. Nine patients had a single episode with a high EBV load (more than 4000 EBV copies/μg peripheral blood mononuclear cell [PBMC] DNA), and 16 patients had high EBV loads detected on 2 or more occasions. Only 8 of these developed symptoms consistent with PTLD, and all were promptly and successfully treated with EBV-specific cytotoxic T cells or CD20 monoclonal antibody. Hence, quantitative measurement of EBV DNA may best be used to enable the prompt rather than the preemptive treatment of PTLD.

scholarly journals Effects of Ovarian Steroids on Immunoglobulin-Secreting Cell Function in Healthy Women

2003 ◽  
Vol 10 (5) ◽  
pp. 944-949 ◽  
Author(s):  
Fabien X. Lü ◽  
Zhongmin Ma ◽  
Susie Moser ◽  
Thomas G. Evans ◽  
Christopher J. Miller
Keyword(s):  
Mononuclear Cells ◽  
Lymphocyte Subsets ◽  
Cell Function ◽  
Viral Infections ◽  
Immunoglobulin A ◽  
Secreting Cell ◽  
Peripheral Blood Mononuclear ◽  
Ovarian Steroids ◽  
Healthy Women ◽  
Immune Mediated

ABSTRACT To determine the effect of the ovarian hormone cycle on immunity, immunoglobulin-secreting cell (ISC) frequency and lymphocyte subsets were examined in the blood of healthy women. We found that immunoglobulin A (IgA)-secreting cells (IgA-ISC) were fourfold more frequent than IgG-ISC in peripheral blood mononuclear cells (PBMC). Further, the ISC frequency in PBMC was highest (P < 0.05) during the periovulatory stage of the menstrual cycle. Thus, endogenous ovarian steroids regulate the ISC frequency and this may explain why women are more resistant to viral infections and tend to have more immune-mediated diseases than men do.

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