Phenotypic Characterization, Genetic Analysis and Antibiotic Sensitivity of Aeromonas Hydrophila Isolates Causing Abdominal Dropsy in Labeo Rohita Collected From Punjab, Pakistan

2021 ◽  
Author(s):  
Fatima Sughra ◽  
Hafeez-ur-Rahman Muhammad ◽  
Farzana Abbas ◽  
Imran Altaf ◽  
Akram Muhammad
Keyword(s):  
Labeo Rohita ◽  
Antibiotic Sensitivity ◽  
Phenotypic Characterization ◽  
Fresh Water Fish ◽  
Fish Disease ◽  
Fish Farms ◽  
Farmed Fish ◽  
Antibiotic Sensitivity Test ◽  
Primary Identification ◽  
Polymerase Chain

Abstract Motile Aeromonas Septicaemia (MAS) is a common fresh water fish disease and major threat to the aquaculture in Pakistan. The present study was carried out on suspected cases of Rohu (Labeo rohita) to characterize and genetically analyze local strains of Aeromonas (A.) hydrophila, a key pathogen responsible for the said disease in farmed fish of Pakistan. A total of ninety suspected samples were collected from fish farms in various districts of Punjab from June 2018 to April 2019. The samples were processed and A. hydrophila strains were isolated. The primary identification of sixty seven isolates was verified by colony morphology, microscopy and phenotypic characterization with ten biochemical reactions. The A. hydrophila strains of test samples were characterized by polymerase chain reaction (PCR) using 16S rRNA at desired size of 356 bp. The PCR amplified product was subjected to DNA sequencing and phylogenetic analysis showed homology with related strains of Aeromonas spp. By antibiotic sensitivity test, the isolates were checked for nine antibiotics, in which pathogen was sensitive to four and resistant to five drugs. Results of molecular characterization confirmed strains as A. hydrophila which are useful to take preventive measures against the said disease.

Isolation, identification, molecular detection and sensitivity to antibiotics of Salmonella from cattle faeces

2021 ◽  
Vol 24 (1) ◽  
pp. 57-66
Author(s):  
M. N. K. Khan ◽  
M. R. Das ◽  
M. A. Sabur ◽  
M. M. Rahman ◽  
M. B. Uddin ◽  
...  
Keyword(s):  
Antibiotic Sensitivity ◽  
Culture Method ◽  
Sensitivity Study ◽  
Culture Methods ◽  
Isolation And Identification ◽  
Conventional Culture ◽  
Faecal Samples ◽  
Antibiotic Sensitivity Test ◽  
Cattle Faeces ◽  
Polymerase Chain

The present study was designed with the aim of isolation and identification of Salmonella by con-ventional culture method and their confirmation by polymerase chain reaction (PCR). Antibacte-rial sensitivity study of isolated Salmonella from cattle faeces was also performed. During the study period of July 2017 to June 2018, a total of 200 faecal samples were collected from different government and private farms in Sylhet district of Bangladesh. Out of 200 samples, 24 (12%) were found to be positive for Salmonella by conventional culture methods. Among the twenty four suspected colonies of Salmonella, seventeen were confirmed by biochemical test and same number was detected by PCR estimating a prevalence of 8.5% (17/200). The prevalence was high-er in calves under 1 year of age (16%) compared with older animals (11.25% of 1–2 years; 10% of above 2 years of age) but without statistically significant differences (χ2=4.835, P=0.089). Moreo-ver, in diarrhoeic animals the prevalence was significantly higher (32.14%, χ2=49.414, P<0.01) than in apparently healthy animals (8.72%). The antibiotic sensitivity test showed that highest number of Salmonella isolates were sensitive to ciprofloxacin (100%), gentamicin (100%) and neomycin (100%). On the other hand, significantly high resistance of Salmonella isolates was detected to erythromycin (100%), amoxicillin (100%), cotrimoxazole (81.48%), streptomycin (62.96%) followed by tetracycline (55.56%).

scholarly journals Isolation and Association of Escherichia Coli AIDA-I/STb, Rather than EAST1 Pathotype, with Diarrhea in Piglets and Antibiotic Sensitivity of Isolates

2003 ◽  
Vol 15 (3) ◽  
pp. 242-252 ◽  
Author(s):  
Musangu Ngeleka ◽  
Jane Pritchard ◽  
Greg Appleyard ◽  
Dorothy M. Middleton ◽  
John M. Fairbrother
Keyword(s):  
Escherichia Coli ◽  
Dna Hybridization ◽  
Antibiotic Sensitivity ◽  
Virulence Determinants ◽  
Shiga Toxins ◽  
E Coli ◽  
Heat Stable ◽  
Antibiotic Sensitivity Test ◽  
Polymerase Chain ◽  
Important Marker

To identify emerging Escherichia coli that have the potential to cause diarrhea in pigs, the prevalence of E. coli pathotypes was determined among 170 and 120 isolates from diarrheic and nondiarrheic piglets, respectively. The isolates were tested for F4, F5, F6, F18, and F41 fimbriae, for E. coli attaching and effacing (EAE), porcine attaching and effacing–associated (Paa), and adhesin involved in diffuse adherence (AIDA-I) factors, for LT, STa, STb, and enteroaggregative heat-stable (EAST1) enterotoxins, and for Shiga toxins (Stx1, Stx2, and Stx2e), using DNA hybridization and polymerase chain reaction. All isolates were O-serotyped and tested for antibiotic resistance against 10 drugs. Seventeen different pathotypes, accounting for 40.0% of the isolates, were recovered from diarrheic piglets. The main pathotypes included EAST1 (13.5%), F4/LT/STb/EAST1 (6.5%), AIDA-I/STb/EAST1 (4.1%), F5/STa (2.9%), EAE/EAST1 (2.9%), and AIDA-I/F18 (2.3%). Only 3 pathotypes, EAE (11.7%), EAST1 (10.8%), and EAE/EAST1 (3.3%), were recovered from nondiarrheic piglets. Paa factor was detected in 8.8% and 7.5% of isolates from diarrheic and nondiarrheic piglets, respectively, and always was associated with other virulence determinants. Overall, 22.9% of isolates from diarrheic piglets appeared to be enteropathogens: enterotoxigenic E. coli (11.7%), enteropathogenic E. coli (3.5%), and E. coli isolates (3.0%) for which none of the above adherence factors was detected. Pathotypes AIDA-I/STb/EAST1 and AIDA-I/STb were isolated only from diarrheic piglets and accounted for 4.7% of isolates. Strains of these pathotypes induced diarrhea when inoculated into newborn colostrum-deprived pigs, in contrast to an isolate positive only for EAST1, which did not induce diarrhea. Antibiotic sensitivity test showed that isolates of the AIDA-I/STb/EAST1 and AIDA-I/STb pathotypes were the only strains sensitive to enrofloxacin, gentamicin, neomycin, and trimethoprim–sulfamethoxazole. This study showed that at least 20.5% of isolates from diarrheic piglets appeared to be associated with AIDA-I/STb pathotype and that EAST1 pathotype is probably not an important marker for diarrhea in piglets.

scholarly journals Bacteriological and molecular study of Pseudomonas aeruginosa strains isolated from different clinical cases in Erbil and Kurkuk

2018 ◽  
Vol 41 (2) ◽  
pp. 124-130
Author(s):  
Asif Hasan Abdul Razzaq
Keyword(s):  
Molecular Weight ◽  
Polymerase Chain Reaction ◽  
Virulence Genes ◽  
Antibiotic Sensitivity ◽  
Molecular Study ◽  
Clinical Samples ◽  
Chain Reaction ◽  
Biochemical Tests ◽  
Antibiotic Sensitivity Test ◽  
Polymerase Chain

     This study included isolates of bacteria from 125 clinical samples in Erbil and Kirkuk Hospital including (burns, wounds, urine and sputum); 38 isolates were identified as P. aeruginosa after conducting microscopic and biochemical tests. The results of antibiotic sensitivity test showed that all isolates of P. aeruginosa  were different in resistance to Pipracillin, Erythromycin with rate of (100%) and to the Nalidixic acid (94.73%) while the lowest resistant antibiotics were to Co-trimoxazole, Ceftazidime and Ciprofloxacin, which amounted to (26.31%, 23.68 and 21.05%) respectively. For molecular diagnosis of P. aeruginosa some virulence genes the alg D and exo A were amplified through Polymerase Chain Reaction technique. The results showed that in 38 isolates cases only 22 (57.9%) were positive for algD gene by amplification of 520 bp band. While in urinary tract infection; 6 samples (60%) had alg D gene, and 8 (57.14%) isolates had alg D gene in wounds samples; also 7(70%) isolates from burns had that gene, while the sputum samples showed only one with alg D gene which was the lowest ratio; but in amplification of exo A, the results showed the presence of only one isolate from burns with molecular weight 396 bp with no appearance in others. 

A RETROSPECTIVE STUDY ON ANTIBIOTIC USAGE IN A TERTIARY CARE HOSPITAL

2020 ◽  
Vol 4 (6) ◽  
Author(s):  
Anna Joy ◽  
Aparna Anand ◽  
Arathy R Nath ◽  
Meera S Nair ◽  
Dr. K. G. Prasanth
Keyword(s):  
Retrospective Study ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
Actual Process ◽  
Care Hospital ◽  
Rational Use ◽  
Antibiotic Susceptibility Test ◽  
Antibiotic Sensitivity Test ◽  
Rational Use Of Antibiotics

Antibiotics are one of the most commonly prescribed drugs today. Rational use of antibiotics is therefore extremely important as their injudicious use can adversely affect the patient. Drug Utilization Evaluation (DUE) is a system of ongoing systematic criteria based evaluation of drug that will help to ensure that medicines are used appropriately. It is drug/disease specific and can be structured so that it will assess the actual process of prescribing, dispensing, or administration of drug. The retrospective study was conducted At Pk Das Institute of Medical Sciences, Palakkad, Kerala for a duration of 6 months (February 2017 - January 2018). A source of data includes Patient case sheets &medication charts, nursing charts, culture & sensitivity reports. The inclusion criterion includes Patients aged between 18- 80 year, prescribed with oral and parenteral antibiotics. . On analyzing the gender, male gender (n= 111, 55.5%) were higher in numbers as compared to female counterparts (n=89, 44.5%). In our study the majority of the patients prescribed with antibiotics were with the clinical assessment of COPD (n=39, 19.5%), UTI (n=37, 18.5%) and LRTI (n=28, 14%), Bronchial asthma (n=19, 9.5%) respectively. On analyzing the data based on antibiotic sensitivity test, antibiotic test were performed and followed in (n=64,32%)prescriptions and in (n=47,23.5%)prescriptions were test is not followed respectively. In (n=89,44.5%) prescriptions, antibiotic sensitivity test is not performed. On analysis of antibiotics prescribed, the most commonly prescribed antibiotics were cephalosporins, of these ceftriaxone was highly prescribed of all (n=95). The high percentage of antibiotic prescriptions may indicate a high probability of irrational use. This study also point out irrational use of antibiotics are more leading to resistance, misuse and serious problems. So certain strategies should be put forward to strengthen rational use of antibiotics. Keywords: Antibiotics, Antibiotic Susceptibility Test, Irrational use, Resistance

scholarly journals Pediatric Septic Arthritis of the Knee Due to a Multi-Sensitive Streptococcus pyogenes Strain Responsive to Clindamycin—A Case Report

Children ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 189
Author(s):  
Giada Maria Di Pietro ◽  
Irene Maria Borzani ◽  
Sebastiano Aleo ◽  
Samantha Bosis ◽  
Paola Marchisio ◽  
...  
Keyword(s):  
Septic Arthritis ◽  
Streptococcus Pyogenes ◽  
Antibiotic Sensitivity ◽  
Antimicrobial Treatment ◽  
Antibiotic Regimen ◽  
Hematogenous Spread ◽  
Clinical Resistance ◽  
Antibiotic Sensitivity Test ◽  
Clinical Conditions

Septic arthritis is an inflammatory process usually generated by a bacterial infection. The knee is one of the most frequently involved joints. The etiology varies depending on age, and hematogenous spread remains the primary cause in children. Herein, we report a case of a previously healthy three-year-old female who was referred to our institution for acute swelling of her right knee. After a clinical and radiological diagnosis of septic arthritis, an empirical treatment with a combination of cefotaxime and clindamycin was initiated. The isolation of a multi-sensitive Streptococcus pyogenes strain from the joint’s effusion prompted the discontinuation of clindamycin and the usage of cefotaxime alone. One week later, an ultrasound was executed due to worsening in the patient’s clinical conditions, and an organized corpuscular intra-articular effusion with diffuse synovial thickening was revealed. Cefotaxime was therefore replaced with clindamycin, which improved the symptoms. Despite the antibiotic sensitivity test having revealed a microorganism with sensitivity to both cephalosporin and clindamycin, clinical resistance to cefotaxime was encountered and a shift in the antimicrobial treatment was necessary to ensure a full recovery. This case study confirms that an antibiotic regimen based solely on a susceptibility test may be ineffective for such cases.

Antibiotic susceptibility testing of bacteria in drinking water sources in Akungba‐Akoko, Ondo State, Nigeria

2011 ◽  
Vol 1 (4) ◽  
pp. 233-237
Author(s):  
A. O. Ajayi ◽  
N. F. Agangan
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Multiple Drug Resistance ◽  
Antibiotic Sensitivity ◽  
Water Sources ◽  
Resistant Bacteria ◽  
Multiple Drug ◽  
Antibiotic Resistant ◽  
Antibiotic Sensitivity Test ◽  
The University

In present investigation, the bacteriological analysis and antibiotic sensitivitypattern of drinking water samples collected from different sources ofAkungba Ã¢â‚¬ÂAkoko, Nigeria was done. The antibiotics mainly considered in ourstudy for determining the sensitivity were amongst the commonly used inthis area for treatment of infectious diseases. As a result, the bacteriologicalindex, especially coliform count was observed notably high with 72 x10 1 cfu/ml for stream sample and 26.4 x 10 1 cfu/ml for borehole sample. The majorbacterial isolates identified in the water samples were Staphylococcus sp,E.coli  Ã¢â‚¬Ânegative bacteria wereobserved showing 75% and 65% resistant to Septrin and Amoxicillin respectively.Also, multiple drug resistance was observed for many antibiotics.Therefore, the presence of high amount antibiotic resistant bacteria of clinicalimportance is reported in these water sources which are usually consumedby students and members of the University community. Hence, thisstudy necessitates the need for water treatment so that epidemics of waterbornebacterial disease can be averted in this region., Klebsiella sp, Pseudomonas sp., Enterococcus sp., Bacillus cereus andothers. With regards to the antibiotic sensitivity test, all isolates showed100% resistance to Ampicillin and Cloxacillin and 85.7% resistance to Zinnacef[a cephalosporin product]. However, the gram 

scholarly journals BACTERIOLOGICAL EXAMINATION OF PUS SAMPLE COLLECTED FROM A COW: A CASE STUDY

2016 ◽  
Vol 4 (12) ◽  
pp. 31-33
Author(s):  
Subha Ganguly
Keyword(s):  
Present Article ◽  
Antibiotic Sensitivity ◽  
Neck Region ◽  
Sensitivity Test ◽  
Bacteriological Examination ◽  
Antibiotic Sensitivity Test

The present article reports on the bacteriological examination and antibiotic sensitivity test of pus sample collected from an abscess at the neck region of a cow.

scholarly journals Analysis of River Water Along Klang Valley to Evaluate the Prevalence of Antibiotics Resistant Strains within Urbanized Areas of Selangor, Malaysia

2020 ◽  
Vol 2 (1) ◽  
pp. 59
Keyword(s):  
River Water ◽  
Antibiotic Sensitivity ◽  
Most Probable Number ◽  
Resistant Bacteria ◽  
Minimal Bactericidal Concentration ◽  
Isolation And Identification ◽  
Antibiotic Resistant ◽  
Probable Number ◽  
Antibiotic Sensitivity Test ◽  
Klang Valley

The determination of antibiotic-resistant bacteria in Klang river water in Klang valley is performed as the river exposed to various environments. The analysis is performed through enumeration, isolation, and identification process. The water samples were obtained from the origin of the river, housing region, and hospital region. The coliforms obtained through enumeration and identification was then used to determine antibiotic sensitivity, minimum inhibitory concentration (MIC), and minimal bactericidal concentration (MBC). The level of coliforms was indicated through the most probable number (MPN), which 70 MPN per 100 ml of river water in the origin of the river while housing and hospital regions showed more than 1600 MPN per 100 ml of river water. The results obtained from the antibiotic sensitivity test showed that the degree of resistance of coliforms is varied in different regions. The zone of inhibition to ampicillin and tetracyclin for coliforms in housing regions is 20 mm, while the coliforms in the hospital region are 6 mm and 7 mm, respectively. The overall results showed that the level of coliforms and the antibiotic sensitivity of coliforms are different in various regions. The coliforms in the hospital region are more resistant to antibiotics compared to the housing region.

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