scholarly journals Effects of Lactobacillus plantarum BCC65951 inoculation in Napier Pakchong 1 silage on in vitro rumen degradability and growth performance of Brahman cattle

2020 ◽  
Author(s):  
Saowaluck Yammuen-art ◽  
Soparak Khemarach ◽  
Kattareeya Taja ◽  
Vethachai Plengvidhya ◽  
Ittiphon Phaopaisal ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Body Weight Gain ◽  
Dry Mass ◽  
Gas Production ◽  
Silage Fermentation ◽  
Daily Body Weight ◽  
Ruminal Degradation ◽  
Silage Production

Abstract Background: Napier grass Pakchong 1 is used as a fodder crop in Southeast Asia. Unfortunately, its fermentation for silage production is challenging owing to the low dry mass content and epiphytic lactic acid bacteria. Here, Lactobacillus plantarum BCC65951 (LAB) inoculation was tested for impact on silage fermentation and use. Silage was prepared with or without inoculation of LAB and stored for 180 days prior to analysis.Results: Gas production at 4-24 h was significantly higher in LAB group (P=0.001). Therefore, the result implied the faster ruminal degradation of LAB, especially during the first 24 h. LAB-inoculated silage exhibited greater levels of lactic acid and in vitro rumen degradability compared to control silage, and significantly lower pH and acetic acid content. To investigate the effect of LAB inoculation on animal performance, ten Brahman bulls were fed on control or LAB-inoculated silage in a crossover experimental design. Animals fed with LAB-inoculated silage had significantly higher voluntary roughage intake and greater average daily body weight gain than those fed with control silage.Conclusions: Results from this study revealed the beneficial effect of Napier Pakchong 1 ensiled with L. plantarum BCC65951 inoculation, both on the fermentative quality of the silage and also the performance of animals fed with the inoculated silage.

Effect of inoculant and enzyme additives on fermentation characteristics and gas production of grass silage

1999 ◽  
Vol 1999 ◽  
pp. 145-145 ◽  
Author(s):  
Z.S. Davies ◽  
A.E. Brooks ◽  
M.K. Theodorou ◽  
G.W. Griffith ◽  
R.J Merry
Keyword(s):  
Lactic Acid ◽  
Cell Walls ◽  
Gas Production ◽  
Natural Process ◽  
Fermentable Sugar ◽  
Plant Cell Walls ◽  
Grass Silage ◽  
Silage Fermentation ◽  
Control Water

Inoculants containing lactic acid bacteria, added to herbage at time of cutting, aid the natural process of fermentation to produce lactic acid during ensilage. This lowers the pH and preserves the silage. It is also claimed that enzyme additives break down polysaccharides in plant cell walls, releasing fermentable sugar to stimulate fermentation and increase the digestibility of the silage. This study was carried out to investigate the effects of inoculants and enzymes on silage fermentation characteristics and gas production during in vitro fermentation.A second cut of perennial ryegrass (Lolium perenne) was mown, chopped to 2 – 4 cm lengths, divided into aliquots and treated at a rate of 10 ml kg-1. The treatments were: 1) control (water), 2) inoculant (Pediococcus pentosaceus, Lactobacillus plantarum and Propionibacter jensenii at 1.6 x 105 cells g-1 herbage) 3) enzyme (xylanase, β-glucanase and amylase, applied at a rate of 0.001 g kg-1 herbage) and 4) inoculant + enzyme.

scholarly journals Evaluación nutricional del ensilaje de Sambucus peruviana, Acacia decurrens y Avena sativa

2005 ◽  
Vol 6 (2) ◽  
pp. 81
Author(s):  
Gloria Milena Blanco ◽  
Diego Rosendo Chamorro ◽  
Luis Carlos Arreaza ◽  
Ana Maria Rey
Keyword(s):  
Lactic Acid ◽  
Avena Sativa ◽  
Gas Production ◽  
Enzymatic Extract ◽  
Mean Values ◽  
Des Moines ◽  
Nutritional Characteristics ◽  
Ruminal Degradation ◽  
Colonization Time

<p>Se evaluaron seis ensilajes mixtos de una gramínea y dos especies arbóreas (<em>Avena sativa, Acacía decurrens, Sambucus peruviana</em>) con la inclusión de melaza, ácido fórmico al 85%, extracto crudo enzimático de fluido ruminal y Kem Lac®*, en microsilos de PVC. Se analizó la eficiencia de la fermentación la composición química y la producción de gas in vitro con el fin de establecer los parámetros de degradación ruminal mediante el modelo matemático exponencial Y= a*{1- exp [-b*(X-c)]]. A los 45 días de fermentación se encontraron diferencias significativas (P&lt;0,01) entre los tratamientos con valores promedio de MS de l8%,  pH 3.84 N-NH3 5,49%, ácido acético 1,55%, ácido láctico 0,34%; no se constató la presencia de los ácidos butírico y propiónico. El tratamiento con adición de extracto enzimático ruminal presentó las mejores características nutricionales; esta respuesta se relaciona con el aporte de nutrientes y la eficiencia en su utilización: una DIVMS de 70,86% asociada con un reducido tiempo de colonización (O25 h), menor FDA (23,27%), contenidos mayores de hemicelulosa (21,13%) y mayor porcentaje de proteína protegida B3 (25,22%) (P &lt;0,01), efectos debidos posiblemente a que el extracto enzimático ruminal facilitó la biodisponibilidad de los polisacáridos para su fermentación a nivel ruminal.</p><p>*Kem-Lac@ es un compuesto de bacterias ácidolácticas y enzimas que favorece la fementación in vitro, en este caso en los microsilos. Kemin Industries, Inc. 2100 Maury St., Box 70. Des Moines, AI. USA. 50301-0070.5151226-2717.</p><p><strong>Nutritional evaluation of Sambucus perviana, Acacia decurrens and, Avena sativa silage </strong></p><p>Six mixed silages with grasses and arboreal species were evaluated using Avena sativa, Acacia decurrens, Sambucus  peruaiana with molasses and 85% formic acid, enzymatic crude extract of ruminal fluid and Kem-Lac®* additives, by means of PVC microsilos. Fermentation efficienry chemical composition and in aitro gas production were analysed for establishing parameters for ruminal degradation by means of Y= a*{1- exp [-b*(X-c)]] exponential mathematical model. After 45 days' fermentation there were significant differences (P&lt;0.01) between the treatments in terms of mean values for MS (18%), pH (3.84%), NNH3 (5.49%), acetic acid (1.55%), 0.34% lactic acid and the absence of butyric or propionic acid. The treatment having added ruminal enzymatic extract displayed the best nutritional characteristics, response related to nutrient contribution and efficiency in use: 70.86% DWMS, smaller colonization time (0.25h), less FDA (23.27%) greater hemicellulose content (21,.13%) and greatest insoluble but digestible protein (B3) percentage (25.22%) (P&lt;0.01). These effects were possibly associated with ruminal enzymatic extract facilitating bio-availability of polysaccharides for their fermentation at ruminal level.</p><p>*Kem-Lac@ is a compound of lactic acid bacteria and enzymes, favors in vitro fementación, in this case in the microsilos. Kemin Industries, Inc. 2100 Maury St., Box 70. Des Moines, AI. USA. 50301-0070.5151226-2717.</p>

scholarly journals Improving ensiling characteristics by adding lactic acid bacteria modifies in vitro digestibility and methane production of forage-sorghum mixture silage

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chatchai Kaewpila ◽  
Pongsatorn Gunun ◽  
Piyawit Kesorn ◽  
Sayan Subepang ◽  
Suwit Thip-uten ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Nutritive Value ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Acid Fermentation ◽  
Design Factor ◽  
Forage Sorghum ◽  
Randomized Design

AbstractImproving the nutrition of livestock is an important aspect of global food production sustainability. This study verified whether lactic acid bacteria (LAB) inoculant could promote ensiling characteristics, nutritive value, and in vitro enteric methane (CH4) mitigation of forage sorghum (FS) mixture silage in attacking malnutrition in Zebu beef cattle. The FS at the soft dough stage, Cavalcade hay (CH), and cassava chip (CC) were obtained. The treatments were designed as a 4 × 2 factorial arrangement in a completely randomized design. Factor A was FS prepared without or with CH, CC, and CH + CC. Factor B was untreated or treated with Lactobacillus casei TH14. The results showed that all FS mixture silages preserved well with lower pH values below 4.0 and higher lactic acid contents above 56.4 g/kg dry matter (DM). Adding LAB boosted the lactic acid content of silages. After 24 h and 48 h of in vitro rumen incubation, the CC-treated silage increased in vitro DM digestibility (IVDMD) with increased total gas production and CH4 production. The LAB-treated silage increased IVDMD but decreased CH4 production. Thus, the addition of L. casei TH14 inoculant could improve lactic acid fermentation, in vitro digestibility, and CH4 mitigation in the FS mixture silages.

scholarly journals Probiotic Potential of a Novel Vitamin B2-Overproducing Lactobacillus plantarum Strain, HY7715, Isolated from Kimchi

2021 ◽  
Vol 11 (13) ◽  
pp. 5765
Author(s):  
Joo-Yun Kim ◽  
Eun-Jung Choi ◽  
Jae-Ho Lee ◽  
Myeong-Seok Yoo ◽  
Keon Heo ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Probiotic Strain ◽  
Optimal Growth ◽  
Control Group ◽  
High Survival ◽  
Vitamin B2 ◽  
Riboflavin Deficiency

Vitamin B2, also known as riboflavin, is essential for maintaining human health. The purpose of this study was to isolate novel lactic acid bacteria that overproduce vitamin B2 and to validate their potential as probiotics. In this study, Lactobacillus plantarum HY7715 (HY7715) was selected among lactic acid bacteria isolated from Kimchi. HY7715 showed a very high riboflavin-producing ability compared to the control strain due to the high expression of ribA, ribB, ribC, ribH, and ribG genes. HY7715 produced 34.5 ± 2.41 mg/L of riboflavin for 24 h without consuming riboflavin in the medium under optimal growth conditions. It was able to produce riboflavin in an in vitro model of the intestinal environment. In addition, when riboflavin deficiency was induced in mice through nutritional restriction, higher levels of riboflavin were detected in plasma and urine in the HY7715 administration group than in the control group. HY7715 showed high survival rate in simulated gastrointestinal conditions and had antibiotic resistance below the cutoff MIC value suggested by the European Food Safety Authority; moreover, it did not cause hemolysis. In conclusion, HY7715 could be considered a beneficial probiotic strain for human and animal applications, suggesting that it could be a new alternative to address riboflavin deficiency.

Lactobacillus plantarum ZJ316 improves the quality of Stachys sieboldii Miq. pickle by inhibiting harmful bacteria growth and degrading nitrite, promoting the gut microbiota health in vitro

2022 ◽  
Author(s):  
Shu Ting Hang ◽  
Ling zhou Zeng ◽  
Jia run Han ◽  
Zhong qin Zhang ◽  
Qingqing Zhou ◽  
...  
Keyword(s):  
Quality Control ◽  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Microbial Contamination ◽  
Nitrite Accumulation ◽  
Bacteria Growth ◽  
Lactobacillus Plantarum Zj316 ◽  
Stachys Sieboldii

Microbial contamination and nitrite accumulation are two major concerns on the quality control of fermented vegetables. In the present study, a lactic acid bacteria strain Lactobacillus plantarum ZJ316 (ZJ316) was...

scholarly journals In Vitro Evaluation of Probiotic Properties of Lactic Acid Bacteria Isolated from Some Traditionally Fermented Ethiopian Food Products

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Guesh Mulaw ◽  
Tesfaye Sisay Tessema ◽  
Diriba Muleta ◽  
Anteneh Tesfaye
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Lactobacillus Plantarum ◽  
Pathogenic Bacteria ◽  
Survival Rates ◽  
Lactobacillus Paracasei ◽  
Probiotic Properties ◽  
Sequence Comparisons

Probiotics are live microorganisms which when consumed in large number together with a food promote the health of the consumer. The aim of this study was to evaluate in vitro probiotic properties of lactic acid bacteria (LAB) isolated from traditional Ethiopian fermented Teff injera dough, Ergo, and Kocho products. A total of 90 LAB were isolated, of which 4 (4.44%) isolates showed 45.35–97.11% and 38.40–90.49% survival rates at pH values (2, 2.5, and 3) for 3 and 6 h, in that order. The four acid-tolerant isolates were found tolerant to 0.3% bile salt for 24 h with 91.37 to 97.22% rate of survival. The acid-and-bile salt-tolerant LAB isolates were found inhibiting some food-borne test pathogenic bacteria to varying degrees. All acid-and-bile-tolerant isolates displayed varying sensitivity to different antibiotics. The in vitro adherence to stainless steel plates of the 4 screened probiotic LAB isolates were ranged from 32.75 to 36.30% adhesion rate. The four efficient probiotic LAB isolates that belonged to Lactobacillus species were identified to the strain level using 16S rDNA gene sequence comparisons and, namely, were Lactobacillus plantarum strain CIP 103151, Lactobacillus paracasei subsp. tolerans strain NBRC 15906, Lactobacillus paracasei strain NBRC 15889, and Lactobacillus plantarum strain JCM 1149. The four Lactobacillus strains were found to be potentially useful to produce probiotic products.

scholarly journals Low-Carbohydrate Tolerant LAB Strains Identified from Rumen Fluid: Investigation of Probiotic Activity and Legume Silage Fermentation

2020 ◽  
Vol 8 (7) ◽  
pp. 1044
Author(s):  
Palaniselvam Kuppusamy ◽  
Dahye Kim ◽  
Ilavenil Soundharrajan ◽  
Hyung Soo Park ◽  
Jeong Sung Jung ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Rumen Fluid ◽  
Lactic Acid Production ◽  
Antagonistic Activity ◽  
Probiotic Properties ◽  
Trifolium Incarnatum ◽  
Crimson Clover ◽  
Silage Fermentation ◽  
Low Carbohydrate

The objective of this study was to isolate and characterize lactic acid bacteria (LAB) with low carbohydrate tolerance from rumen fluid and to elucidate their probiotic properties and the quality of fermentation of Medicago sativa L. and Trifolium incarnatum L. silage in vitro. We isolated 39 LAB strains and screened for growth in MRS broth and a low-carbohydrate supplemented medium; among them, two strains, Lactiplantibacillus plantarum (Lactobacillus plantarum) RJ1 and Pediococcus pentosaceus S22, were able to grow faster in the low-carbohydrate medium. Both strains have promising probiotic characteristics including antagonistic activity against P. aeruginosa, E. coli, S. aureus, and E. faecalis; the ability to survive in simulated gastric-intestinal fluid; tolerance to bile salts; and proteolytic activity. Furthermore, an in vitro silage fermentation study revealed that alfalfa and crimson clover silage inoculated with RJ1 and S22 showed significantly decreased pH and an increased LAB population at the end of fermentation. Also, the highest lactic acid production was noted (p < 0.05) in LAB-inoculated silage vs. non-inoculated legume silage at high moisture. Overall, the data suggest that RJ1 and S22 could be effective strains for fermentation of legume silage.

Effects of adding a Lactobacillus Plantarum inoculant to grass ensiled at different dry matter concentrations and offered to beef cattle

1994 ◽  
Vol 1994 ◽  
pp. 115-115 ◽  
Author(s):  
P. O'Kiely
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Beef Cattle ◽  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Nutritive Value ◽  
Silage Fermentation ◽  
Different Levels

Silage fermentation is progressively restricted as the extent of pre-wilting increases (O'Kiely et_al., 1988). The magnitude of the improvement in silage nutritive value in response to a lactic acid bacteria inoculant could be related to the extent of the fermentation in the untreated silage. The objective of this experiment was to determine if the response in silage nutritive value to a Lactobacillus plantarum inoculant was similar at different levels of dry matter (DM) concentration.

Sign in / Sign up

Export Citation Format

Share Document