scholarly journals The Association between Polymorphism of NLRP3 rs10754558 and Susceptibility to Arteriosclerosis Obliterans in Chinese Han Male Population

2021 ◽  
Author(s):  
Kun Zhang ◽  
Wei Song ◽  
Jiaxuan Feng ◽  
Haoshan Qi ◽  
Mingjin Guo
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Control Group ◽  
Genotype Distribution ◽  
Male Population ◽  
Chinese Han ◽  
Cholesterol Crystals ◽  
Arteriosclerosis Obliterans ◽  
The Relationship

Abstract Background and aims: Cholesterol crystals have been proved to be able to cause inflammation. NLRP3 inflammasome is activated in response to the accumulation of cholesterol crystals to produce IL-1β, which is associated with atherosclerotic lesions. NLRP3, as part of innate immunity, is involved in the regulation of inflammatory activity described above. The main objective of this study was to investigate the relationship between the polymorphism of NLRP3 rs10754558 and the susceptibility to arteriosclerosis obliterans (ASO) in Chinese Han males. Methods: The NLRP3 rs10754558 genotype was detected by the TaqMan allele assays in 758 male patients suffered from arteriosclerosis obliteration and 793 male controls. Blood glucose, total cholesterol (TC), triglyceride (TG), urea nitrogen, creatinine, serum uric acid, high-density lipoprotein, low-density lipoprotein, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and IL-1β were detected in all subjects. Clinical data were recorded and genotype-phenotype was analyzed. Independent sample T test was used for comparison between the two groups. The odds ratio (ORs) with 95% confidence interval (CIs) was calculated to show the strength of the relationship between the genotype distribution or allele frequencies and ASO. Genotype-phenotype analysis was performed in ASO patients by ANOVA. Results: The frequencies of genotype and allele in ASO group were significantly different from that in control group (p = 0.022 by genotype, p = 0.003 by allele). People who were carrying genotype GG had a higher risk for ASO than those carrying genotype CC (OR=1.574,95％CI 1.161-2.135,P=0.003) ,which was still significant after the adjustment of the history of smoking, TC, LDL, fasting blood glucose, systolic blood pressure and BMI(OR=1.448,95％CI 1.046-1.461,P=0.004). Moreover, there was an interaction between rs10754558 of NLRP3 and rs2043211 of CARD8 gene. Under the premise of carrying the T allele of CARD8 rs2043211, the G allele of NLRP3 rs10754558 increases the susceptibility to ASO. This gene-gene interaction is consistent with IL-1β levels. Conclusion: Our finding suggests the polymorphisms of NLRP3 rs10754558 are probably associated with the development of ASO in Chinese Han male population. And there may be an interaction between rs10754558 of NLRP3 and rs2043211 of CARD8 gene in the development of ASO.

scholarly journals The Association between Polymorphism of CARD8 rs2043211 and Susceptibility to Arteriosclerosis Obliterans in Chinese Han Male Population

2017 ◽  
Vol 41 (1) ◽  
pp. 173-180 ◽  
Author(s):  
Kun Zhang ◽  
Wei Song ◽  
Dalin Li ◽  
Jingqiang Yan ◽  
Yunhui Chen ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Primary Objective ◽  
Control Group ◽  
Genotype Distribution ◽  
Male Population ◽  
Chinese Han ◽  
Arteriosclerosis Obliterans

Background and aims: Cholesterol crystals have been shown to cause inflammation. As a response to cholesterol crystal accumulation, the NLRP3 inflammasome is activated to produce IL-1β which eventually leads to atherosclerotic lesions. As a part of innate immunity, CARD8 is involved in the modulation of above mentioned inflammatory activities. The primary objective of this study was to investigate the association between polymorphism of CARD8 rs2043211 and susceptibility to arteriosclerosis obliterans (ASO) in Chinese Han male population. Methods: 758 male arteriosclerosis obliterans patients and 793 male controls were genotyped for rs2043211 with the TaqMan allele assays. Fasting blood-glucose (FBG), total cholesterol (TC), triglycerides (TG), urea nitrogen, creatinine, Serum uric acid, high density lipoprotein, low density lipoprotein, ALT, AST, and IL-1β in the blood were detected for all subjects. Clinical data were recorded to analyze the genotype-phenotype. Independent samples t-test was used to perform the comparisons between two groups. Odds ratios (ORs) with 95% confidence intervals (CIs) were calculated to measure the strength of relationship in the genotype distribution and allele frequencies between patients and controls. The analysis of variance was used for a genotype-phenotype analysis of the ASO patients. Results: The genotypic and allelic frequencies in the ASO group were significantly different from that in the control group (P = 0.014 by genotype, P = 0.003 by allele). Those carrying the genotype TT had a higher risk for ASO than those carrying the genotype AA (OR = 1.494, 95%CI1.131-1.974, P = 0.005).The difference was also significant after the adjustment for the history of smoking, TC, LDL, fasting blood glucose, systolic blood pressure and BMI(OR = 1.525, 95%CI1.158-2.009, P = 0.003). Conclusion: Our finding suggests that the polymorphism of CARD8 rs2043211 is probably associated with the development of ASO in Chinese Han male population.

scholarly journals Effects of Marine Drug Propylene Glycol Alginate Sodium Sulfate on Glucose and Lipid Metabolism in Mice

2021 ◽  
Vol 58 (2) ◽  
pp. 150-154
Author(s):  
Haiyue Liang ◽  
Qun Liu ◽  
Yonghong Xiu
Keyword(s):  
Lipid Metabolism ◽  
Blood Glucose ◽  
Sodium Sulfate ◽  
Glycosylated Hemoglobin ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Control Group ◽  
Distilled Water ◽  
Glucose And Lipid Metabolism ◽  
Propylene Glycol Alginate

Previous studies have shown that marine drug propylene glycol alginate sodium sulfate (PSS) plays important roles in human diseases. This study mainly explored the effects of PSS on hyperglycemia and hyperlipidemia in diabetic db/db mouse models. The db/db mice were randomly divided into 5 groups (n=12), which were model control group (distilled water), positive control group (metformin), PSS low, medium, and high dose groups (PSS25, PSS50, PSS100) and normal control group (C57/BL, distilled water). The mice in each group had free diet and water for 90 days. During the experiment, food intake was recorded every day and body weight was recorded weekly. In addition, fasting blood glucose and glycosylated hemoglobin levels were measured regularly. Finally, the contents of triglyceride (TG), low-density lipoprotein (LDL-c), high-density lipoprotein (HDL-c) and total cholesterol (TC) in the serum of mice were determined. PSS can significantly reduce fasting blood glucose and glycosylated hemoglobin levels in db/db mice, and improve insulin sensitivity. Moreover, PSS can reduce the fat accumulation of db/db mice and significantly improve the blood lipid level of db/db mice. PSS can significantly improve the symptoms of glucose and lipid metabolism disorders in db/db mice.

scholarly journals Effects of Fasting-Mimicking Diet and Specific Meal Replacement Foods on Blood Glucose Control in Patients with Type 2 Diabetes: A Randomized Controlled Trial

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Fang Tang ◽  
Xuan Lin
Keyword(s):  
Type 2 Diabetes ◽  
Blood Glucose ◽  
Venous Blood ◽  
Fasting Blood Glucose ◽  
Blood Glucose Control ◽  
Density Lipoprotein ◽  
Control Group ◽  
Meal Replacement ◽  
Experimental Group

Type 2 diabetes represents a serious societal health problem due to the vulnerability to cardiovascular events. Diet therapy is the most basic treatment for type 2 diabetes. The present study was conducted to study the effect of a fasting-mimicking diet and specific meal replacement foods on blood glucose control in patients with type 2 diabetes. Our study included 100 patients with type 2 diabetes who underwent a physical examination which were enrolled and randomly assigned as 50 patients each to the test group (with low energy-specific meal replacement meals during a fasting-mimicking diet) and the control group (with specific meal replacement foods given normal adult doses). After 4 months, efficacy indicators which were fasting blood glucose, 2-hour postprandial venous blood glucose, and glycosylated haemoglobin of the experimental group were all lower than those of the control group ( P < 0.05 ); observation indicators that include body mass index, waist circumference, blood lipids (triglyceride, cholesterol, and low-density lipoprotein), and blood pressure levels were all lower than the control group, and high-density lipoprotein levels were all higher than the control group (all P < 0.05 ). Both groups of fasting blood glucose, 2-hour postprandial venous blood glucose, and blood pressure had a relatively stable downward trend, but the experimental group had a more significant decline. In conclusion, the study revealed that a fasting-mimicking diet and specific meal replacement foods can safely and effectively reduce weight and improve metabolic syndrome in patients with type 2 diabetes.

scholarly journals Low Expression of FFAR2 in Peripheral White Blood Cells May Be a Genetic Marker for Early Diagnosis of Acute Myocardial Infarction

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Jianjun Ruan ◽  
Heyu Meng ◽  
Xue Wang ◽  
Weiwei Chen ◽  
Xiaomin Tian ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Risk Factor ◽  
Blood Glucose ◽  
Peripheral Blood ◽  
Fasting Blood Glucose ◽  
Control Group ◽  
Fasting Blood Glucose Level ◽  
The Relationship ◽  
Low Expression

Objective. To find molecular markers for the diagnosis of acute myocardial infarction (AMI), this research further verified the relationship between the expression level of FFAR2 gene and AMI by expanding the sample size based on the previous gene chip results. Methods. Peripheral venous leukocytes were collected from 113 patients with AMI and 94 patients with noncoronary artery disease as the experimental group and the control group, respectively. Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of the FFAR2 gene. Western blot analysis was applied to detect the relative expression of the FFAR2 gene at the level of protein. Furthermore, the relationship between gene expression and clinical data was also analyzed and compared. Results. The level of expression of FFAR2 gene in peripheral blood of patients with AMI was significantly lower than that of the control group (0.33 [0.04–1.08], 0.62 [0.07–1.86], respectively; p<0.05), which was 0.53 times that of the control group. Western blot results presented that the FFAR2 protein level in the peripheral blood of the AMI group was lower than that of the control group (0.114; p=0.004). Analyzing clinical data of the subjects indicated that the average age of the AMI group was significantly higher than the age of control group (p<0.01). Also, the fasting blood glucose level was higher (p<0.01), and the high-density lipoprotein cholesterol (HDL-C) level was lower (p=0.03). The FFAR2 mRNA level correlated positively with the HDL-C level (p<0.01). Logistic regression analysis suggested that the low expression of the FFAR2 gene in peripheral blood may be a risk factor for AMI independent of age, family history of diabetes, fasting blood glucose level, and HDL-C level (p=0.025). Compared with the high FFAR2 expression group, the risk of AMI in the low FFAR2 expression group was 6.308 times higher. Conclusion. The expression level of the FFAR2 gene in peripheral blood of patients with AMI was significantly lower than that in the control group. Low expression of the FFAR2 gene in peripheral blood is an independent risk factor for AMI. Hence, it may also be a potential biomarker to predict AMI.

scholarly journals Genotype Distribution of CNDP1 Polymorphisms in the Healthy Chinese Han Population: Association with HbA1c and Fasting Blood Glucose

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Shiqi Zhang ◽  
Juan Xu ◽  
Di Cui ◽  
Shujuan Jiang ◽  
Xin Xu ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Serum Glucose ◽  
Chinese Han Population ◽  
Metabolic Parameters ◽  
Genotype Distribution ◽  
Chinese Han ◽  
Allele Distribution ◽  
Han Population ◽  
Healthy Chinese

We have previously reported that the CNDP1 (CTG)5 allele affords protection against diabetic nephropathy (DN) in patients with Type 2 diabetes (T2DM) of Caucasian origin. Because the incidence of ESRD attributable to both Type 1 diabetes (T1DM) and T2DM is higher among South Asian than Caucasian people, the present study assessed relevant CNDP1 polymorphisms and their association with metabolic parameters in the Chinese Han population. To this end, the (CTG)n allele distribution along with 5 relevant SNPs in the CNDP1 gene, previously reported to be associated with DN in non (CTG)5 carriers of Afro-American ethnicity, were determined in 663 healthy individuals. The (CTG)6 homozygous genotype was the most prevalent (84.5%) genotype in the Chinese Han population. The (CTG)5 and (CTG)4 alleles were present in a small minority of individuals accounting for 15.2% and 0.3% of genotypes with at least one (CTG)5 or one (CTG)4 allele, respectively. Only 0.5% of individuals carried the homozygous (CTG)5 genotype and individuals carrying the homozygous (CTG)4 genotype were not found. The minor allele frequencies (MAFs) of the 5 SNP were 0.197 (C allele for rs4892247), 0.0855 (C allele for rs62099905), 0.085 (G allele for rs62099906), 0.066 (T allele for rs62099907), and 0.18 (A allele for rs72979715). All the SNPs except rs4892247 genotypes were in the Hardy-Weinberg equilibrium. Neither the (CTG)n polymorphism nor the latter three SNPs reached significance when compared with different metabolic parameters. In contrast, individuals with the TT genotype of rs62099905 presented lower fasting blood glucose but higher HbA1c levels. In conclusion, the rs62099905 in the CNDP1 gene is associated with serum glucose levels in the healthy Chinese Han population, while for the CNDP1 (CTG)n polymorphism, no association with serological parameters was found.

scholarly journals Clinical Efficacy and Safety of Yi-Shen-Hua-Shi Granule for Diabetic Kidney Disease: A Cohort Study

2020 ◽  
Author(s):  
Yebei Li ◽  
Zhipeng Yan ◽  
Kang Wang ◽  
Tianlun Huang ◽  
Xin Liu ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Kidney Disease ◽  
Diabetic Kidney Disease ◽  
Adverse Reactions ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Control Group ◽  
Efficacy And Safety ◽  
Diabetic Kidney

Abstract Background: The present study aims to explore the relative efficacy and safety of Yi-Shen-Hua-Shi (YSHS) granule in diabetic kidney disease (DKD) patients. Methods: A total of 260 DKD patients with the urinary albumin-to-creatinine ratio (UACR) ≥ 30 mg/g and estimated glomerular filtration rate (eGFR) > 30 ml/min/1.73 m2 were included. 130 patients receiving YSHS plus irbesartan and 130 patients with irbesartan alone. The primary outcomes were included serum creatinine (Scr), 24-hour urinary protein (24h UTP), UACR, and the overall response rate (ORR). The secondary outcomes included fasting blood glucose (FBG), hemoglobin A1c (HbA1c), total cholesterol (TC), triglyceride (TG), and low-density lipoprotein (LDL-C). Results: At the end of 12 weeks, compared with the controls, the YSHS group could effectively improve the level of 24h UTP (P = 0.007), UACR (P = 0.001), FBG (P = 0.024), HbA1c (P = 0.005), TC (P = 0.018), TG (P = 0.001), and LDL-C (P = 0.034). The ORR in the YSHS group and control group was 47 % versus 30 % at 12 weeks (P = 0.005), respectively. There was no statistical difference in major adverse reactions between the two groups during treatment.Conclusions: The YSHS granule has significant curative effects on DKD, especially in reducing proteinuria, improving blood glucose and lipid metabolism, with no obvious adverse reactions.

scholarly journals Evaluation of serum asprosin levels in women with metabolic syndrome in Duhok City-Kurdistan Region/Iraq

2021 ◽  
Vol 9 (8) ◽  
pp. 2240
Author(s):  
Dhia M. Sulaiman
Keyword(s):  
Metabolic Syndrome ◽  
Blood Glucose ◽  
Demographic Data ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Control Group ◽  
Fasting Insulin ◽  
Kurdistan Region ◽  
Cross Sectional ◽  
Significant Difference

Background: Serum asprosin, a recently discovered hormone as a new adipocytokine, which has been associated with the regulation of both glucose and lipid metabolism, and insulin resistance. Metabolic syndrome considered as a disorder of lipid and glucose metabolism, with impairment in insulin function, which might be associated with serum asprosin, therefore, new researches focused on the role of asprosin in the pathogenesis of metabolic syndrome to clarify such relationship. This study aimed to evaluate serum asprosin levels in women with metabolic syndrome and compared with a woman without metabolic syndrome.Methods: This study was performed at Obstetrics and Gynecology Hospital, and Mazi medical clinics in Duhok, Kurdistan Region-Iraq, the study was established from June, 2020 to January, 2021. In this cross-sectional study, serum asprosin concertation in 40 women with metabolic syndrome were compared with a 131 women without metabolic syndrome. The demographic data were collected, serum asprosin levels, lipid profile, fasting blood glucose, fasting insulin were biochemically analyzed by using the autoanalyzer machine COBASS series 6000 and ELISA technique.Results: The mean age of women with metabolic syndrome was (24.36±3.23) and women without metabolic syndrome was (23.18±3.87), serum aspersion in women with metabolic syndrome was (18.34±5.4) ng/ml, while in women without metabolic syndrome was (7.48±5.82) with significant difference (p<0.001). In study population, there was a positive correlation between asprosin and body mass index, waist circumference, triglyceride, total cholesterol, fasting blood glucose, fasting insulin, and HOMA-IR (p<0.0001), while serum asprosin was negatively correlated with high density lipoprotein- cholesterol (p<0.01).Conclusions: The study confirms that serum asprosin in women with metabolic syndrome was higher than in the control group.  

Analysis of the Effect of Miglitol in the Treatment of Diabetes Mellitus Based on Ag+ Disinfectant Sampling Needle

2020 ◽  
Vol 12 (4) ◽  
pp. 550-555
Author(s):  
Ling Zhao ◽  
Liping Tan ◽  
Guoliang Cheng ◽  
Yuanyuan Miao ◽  
Xiangming Zhou ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Adverse Reactions ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Insulin Level ◽  
Lipoprotein Cholesterol ◽  
Postprandial Blood Glucose ◽  
Control Group ◽  
Diabetic Patients ◽  
Study Group

The present work selected 120 patients, affected by diabetes, as control subjects in the Affiliated Hospital of Kunming Medical University. According to the principle of random distribution, two groups were established, miglitol study group (65 cases) and metformin control group (55 cases). The Ag + disinfectant sampling needle is a rapid detection tool to evaluate the efficacy of diabetes in both groups. The related biochemical indexes of the patients were measured before and after treatment at the 3rd, 6th, and 12th weeks. The test results showed that there were significant differences in fasting blood glucose value (FBG) and postprandial blood glucose value (2hFBG) among the two groups after 3-, 6-, and 12-month treatment. With the extension of treatment, the blood glucose level of diabetic patients gradually stabilized. Fasting insulin level (FINS) and postprandial two-hour insulin level (2hINS) increased gradually. At the 12th week, by measuring the HbA1c level of the patients, it was found decreasing significantly. In detail, the study group decreased more significantly than the control group, and the incidence of adverse reactions during treatment was 10.8% and 23.6%, respectively, with a significant statistical significance (P < 0 05). Meanwhile, we measured other biochemical indexes of patients from both groups after drug treatment. At the 6th week, the levels of glycosylated glycoprotein (HbA1c), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) in the study group decreased more than in the control group. Therefore, miglitol shows a better hypoglycemic effect on type 2 diabetes, and the gastrointestinal adverse reactions are lesser than metformin, which is more worthy in clinical application.

Anti-diabetic activity of diphenhydramine in diabetic rats

2020 ◽  
Vol 11 (4) ◽  
pp. 5067-5070
Author(s):  
Pang Jyh Chayng ◽  
Nurul Ain ◽  
Kaswandi Md Ambia ◽  
Rahim Md Noah
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Group Iv ◽  
Diabetic Rat ◽  
Control Group ◽  
Group I

The purpose of this project is to study the anti-diabetic effect of on a diabetic rat model. A total of Twenty male Sprague rats were used and it randomly distributed into four groups which are Group I: , Group II: negative control, Group III: and Group IV: and . In diabetic model were induced with via injection at the dosage of 65mg/kg. and FBG (Fasting Blood Glucose) level of diabetic rats were assessed every three days. Blood was collected via cardiac puncture at day 21 after the induction of treatment. Insulin level of the rats was assessed with the Mercodia Rat Insulin ELISA kit. FBG level of group I (12.16 ±3.96, p&lt;0.05) and group IV (11.34 ±3.67, p&lt;0.05) were significantly decreased. Meanwhile, the for all rats did not show any significant increase. However, the insulin level was escalated in group IV (0.74+0.25, p&lt;0.05) significantly. The present study shows that the and the combination of and lowered blood glucose level and enhanced insulin secretion.

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