scholarly journals Extraction of Protein Isolates From Grouper (Epinephelus Diacanthus) Filleting Waste Using pH Shift Method; Influence of Process Variables on Recoveries

2021 ◽  
Author(s):  
Uzair Shafiq ◽  
Kirankumar Gopalbai Baraiya ◽  
VIJAY KUMAR REDDY SURASANI ◽  
Sagar Joshi ◽  
Raju Chikoppa Varadaraju
Keyword(s):  
Extraction Process ◽  
Protein Isolate ◽  
Raw Material ◽  
Solvent Ratio ◽  
Process Variables ◽  
Optimum Conditions ◽  
Shift Method ◽  
Protein Isolates ◽  
Ph Shift ◽  
Alkaline Range

Abstract Recovery of proteins from grouper (Epinephelus diacanthus) filleting waste was done using isoelectric solubilization method. During the isolation, influence of process variables such as; temperature, ratio of solvent and homogenate, pH, time of centrifugation and speed of centrifugation on protein yields was studied. It was observed that the homogenate to solvent ratio, centrifugation speed protein recoveries. For isolating the proteins from grouper waste using pH shift process the optimum conditions found were; pH-11.0 in alkaline range, pH 3.0 in acidic range, 120 min extraction time, homogenate to solvent ratio of 1:6 and centrifugation speed of 10000 rpm. During the pH shift processing of grouper proteins myoglobin, total pigments and lipid content of proteins were reduced by 90.77, 70.79 and 82.69%, and 93.91, 79.59 and 82.04%, in alkaline and acidic extraction process, respectively. A significant increase in the lightness and whiteness values of the isolates was observed as compared to the raw material. Protein isolates obtained using acidic solubilisation process were found to be whiter than alkali-aided protein isolate.

PEMBUATAN ZAT WARNA ALAMI DARI BIJI KESUMBA DALAM BENTUK KONSENTRAT TINGGI UNTUK PEWARNA MAKANAN

EKUILIBIUM ◽  
2014 ◽  
Vol 13 (2) ◽  
Author(s):  
Paryanto Paryanto
Keyword(s):  
Sodium Hydroxide ◽  
Water Content ◽  
Negative Impact ◽  
Extraction Process ◽  
Solvent Ratio ◽  
Synthetic Dyes ◽  
Bixa Orellana ◽  
Optimum Conditions ◽  
Natural Pigments ◽  
Maximum Absorbance

<p>Abstract: The use of synthetic dyes for food in Indonesia reached 88%. The synthetic dyes<br />have the negative impact for healthy. This study was conducted to determine how produce bixin<br />natural pigments from annatto seed by extraction process, how the optimum conditions and<br />their application. Bixin extraction from the seeds annatto using acetone (CH<br />3<br />) and<br />sodium hydroxide (NaOH) as solvent. The various of extraction were concentration, extraction<br />temperature, stirring speed and the ratio of material to solvent. The bixin analysis used UV-Vis<br />spectrophotometer at maximum absorbance and FTIR spectrophotometer to determine the<br />groups of bixin. The water content in the bixin seeds is 37%. The optimum conditions of the<br />extraction process of bixin seed is 0.25 N NaOH as solvent, 60<br />C, 400 rpm stirring speed and<br />weight of material to solvent ratio 1:20.<br />Keywords: Extraction, bixin, annatto, bixa orellana, sodium hydroxide<br />o<br />COCH<br />3</p>

Removal of Lipids, Cholesterol, Nucleic Acids and Haem Pigments During Production of Protein Isolates from Broiler Meat Using pH-shift Processes

2017 ◽  
Vol 13 (4) ◽  
Author(s):  
Manat Chaijan ◽  
Worawan Panpipat
Keyword(s):  
Nucleic Acids ◽  
Protein Isolate ◽  
Total Lipids ◽  
Functional Protein ◽  
Broiler Meat ◽  
Protein Isolates ◽  
Ph Shift ◽  
Gelling Properties ◽  
Gel Forming Ability ◽  
Water Holding

Abstract The effect of acid and alkaline pH shift processes on removal of total lipids, cholesterol, nucleic acids and haem pigments during production of protein isolates from broiler meat was investigated. The gel-forming ability of resulting protein isolates were evaluated in comparison with raw broiler meat and water washed broiler meat. Significant reduction of total lipids, cholesterol, nucleic acids and haem proteins was obtained from both pH shift processes (p < 0.05). Acid process recovered more protein with less total haem pigments resulting in a greater breaking force and whiteness of the isolate gel compared to alkaline counterpart (p < 0.05). However, protein isolate gels showed inferior deformation and water holding capacity to washed mince gel (p < 0.05). Therefore, the pH shift processing could be used to produce a functional protein isolate with low nucleic acids, haem pigments and lipids and, thereby, reduced cholesterol level. The protein isolates, particularly acid version, still had good gelling properties.

ZAT WARNA DARI GETAH TANGKAI DAUN PISANG (MUSA SSP.)

EKUILIBIUM ◽  
2015 ◽  
Vol 14 (2) ◽  
Author(s):  
Paryanto Paryanto
Keyword(s):  
Rotational Speed ◽  
Extraction Process ◽  
Natural Dye ◽  
Solvent Ratio ◽  
Optimum Conditions ◽  
Total Level ◽  
Banana Leaf ◽  
Banana Stem ◽  
Material Extraction ◽  
Brown Color

<p>Abstract: One of the natural dye sources for the brown color called tannins is Resin Banana<br />Leaf Stem. Tannins were obtained through the extraction process of Resin Banana Leaf Stem<br />using 96% ethanol. This study aimed to obtain a total level by extraction and drying. The<br />influence of the material with a solvent ratio (weight/volume), time, temperature, and rotational<br />speed stirrer in the extraction of dye from resin stem of banana were studied to get an optimum<br />process. This experiment is using some ratios of the weight of banana leaf to the volume of<br />solvent, i.e.: 20:200 and 50:200 (g/ml). The condition was set at 63<br />C for 160 minutes and<br />stirring rotational speed of 200 rpm. With the same material, extraction with soxhlet was done<br />until the solvent becomes clear. Total tannin was obtained at 7.68%. With a three-neck flask<br />extraction process obtained the optimum conditions, i.e.: the ratio of the weight to the volume of<br />solvent of 50:200 g/ml at 63<br />o<br />o<br />C, and stirring rotational speed of 200 rpm for 140 minutes. This<br />condition process produce heavy tannins about 29.2576 mg.<br />Keywords: banana stem, resin, extraction, natural dye</p>

Optimization of Extraction Process of Lycopene from Watermelon (Citrullus lanatus) by Response Surface Methodology

2011 ◽  
Vol 140 ◽  
pp. 385-393 ◽  
Author(s):  
Xian Feng Shi ◽  
Yuan Xu ◽  
Yu Hua Li ◽  
Hong Xia Zeng ◽  
Yu Hong Sun
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Citrullus Lanatus ◽  
Extraction Process ◽  
Quadratic Equation ◽  
Raw Material ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Extraction Solvent

The extraction processing of lycopene from watermelon was investigated in this paper. Based on single factor experiments, response surface methodology (RSM) was adopted to study the key parameters such as number of extraction, extraction temperature, extraction time in order to increase extraction rate of lycopene. A second order quadratic equation was established and the applicability of model and interaction involved factors on predicting the lycopene extracting content was verified. The results indicated that the optimum extraction conditions were hexane (containing 2% dichloromethane) as extraction solvent, ratio of solvent to raw material of 3:1 (mL/g), number of extraction of 2, extraction time of 1.9 h and extraction temperature of 29.8°C. Under these conditions the extracting content of lycopene was 14.71±0.22 mg/kg, which is well in agreement with value predicted by the model.

scholarly journals Citrus Bioactive Limonoid Extraction using Environment-friendly Hydrotropy

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1008C-1008
Author(s):  
Deepak Dandekar ◽  
G. K. Jayaprakasha ◽  
Bhimanagouda Patil
Keyword(s):  
Extraction Efficiency ◽  
Design Method ◽  
Maximum Yield ◽  
Extraction Process ◽  
Extraction Methods ◽  
Raw Material ◽  
Extraction Temperature ◽  
Optimum Conditions ◽  
Environment Friendly ◽  
Health Promoting

Citrus consumption has been shown to promote human health due to presence of several bioactive compounds. In the process of understanding the health benefits of citrus, we need to isolate and characterize these compounds. Limonoids are one of such prominent, but lesser-known phytonutrients that have been shown to prevent cancers of the mouth, skin, lung, breast, and colon. With the growing interest in the health-promoting properties of citrus limonoids, the demand for these bioactives has significantly increased. It has been critical to explore environment-friendly extraction methods rather than using hazardous organic solvents. A water-based hydrotropic extraction of limonoid aglycones from sour orange (Citrus aurantium L.) seeds was developed. Two hydrotropes, sodium salicylate (Na-Sal) and sodium cumene sulfonate (Na-CuS), were studied for extraction efficiency using the Box Behnken experiment design method. The extraction efficiency of prominent aglycone limonin was observed depending on hydrotrope concentration, extraction temperature, and percentage of raw material loading. Response Surface Analysis (RSA) of data predicted the optimum conditions for maximum yield. Recovery of aglycones from filtered extract is also easily achieved by mere dilution using water at pH 3 or 7 or by partitioning the extract with dichloromethane. At optimum conditions, limonin yield of 0.46 mg/g seeds in the case of Na-Sal extraction and 0.65 mg/g seeds in the case of Na-CuS extraction was achieved. The results demonstrated that the hydrotropic extraction process of limonoid aglycones has practical commercial importance. This project is based upon work supported by the USDA–CSREES IFAFS #2001 52102 02294 and USDA–CSREES #2005-34402-14401 “Designing Foods for Health” through the Vegetable and Fruit Improvement Center.

scholarly journals Research on polyphenols extraction from Polygonum multiflorum Thunb. roots

2020 ◽  
Vol 66 (1) ◽  
pp. 9-17
Author(s):  
Le Pham Tan Quoc
Keyword(s):  
Phenolic Compounds ◽  
Extraction Process ◽  
Raw Material ◽  
Extraction Time ◽  
Initial Structure ◽  
Solvent Ratio ◽  
Water Mixture ◽  
Extraction Temperature ◽  
Polygonum Multiflorum ◽  
Dpph Method

SummaryIntroduction:Polygonum multiflorum Thunb. is a herbal common plant in Asia, with many beneficial health effects for human because it contains many bioactive compounds which can prevent some diseases, for instance such as cardiovascular diseases, cancers, neurodegenerative diseases, etc.Objective: The purpose of this research is to point out the effects of extraction factors such as type of solvent, material/solvent ratio (w/v), solvent concentration (%, v/v), temperature (°C) and extraction time on the extraction yield of phenolic compounds from Polygonum multiflorum Thunb. roots, for instance, total polyphenol content (TPC) and antioxidant capacity (AC).Methods: The raw material consisting of Polygonum multiflorum Thunb root was extracted by the reflux maceration method. TPC and AC of received extract were evaluated by the Folin-Ciocalteu technique and DPPH method with Trolox as a standard agent.Results: The optimal conditions for the extraction process were acetone-water mixture (60%, v/v) as a solvent, material/solvent ratio of 1/40, extraction temperature of 50°C and extraction time of 90 minutes. The surface structure of material after extraction process changed insignificantly compared with the initial structure.Conclusion: The results showed that TPC and AC obtained the best values (38.60±0.56 mg GAE/g DW (dry weight) and 298.15±2.99 μmol TE/g DW, respectively) at optimal extraction conditions. In addition, some phenolic compounds were detected in the extract such as gallic acid, catechin and resveratrol.

scholarly journals Impact of Processing Technology on Macro- and Micronutrient Profile of Protein-Enriched Products from Fish Backbones

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 950
Author(s):  
Mehdi Abdollahi ◽  
Haizhou Wu ◽  
Ingrid Undeland
Keyword(s):  
Protein Isolate ◽  
Processing Technology ◽  
Protein Recovery ◽  
Food Ingredients ◽  
Protein Isolates ◽  
Ph Shift ◽  
Vitamins E And C ◽  
Copper Zinc ◽  
Mechanical Separation ◽  
Mechanically Separated Meat

Impacts of processing technology (mechanical separation and pH-shift processing) on protein recovery from salmon, herring and cod backbones and the content of macro- and micronutrients in the recovered protein enriched products were investigated. Mechanical separation led to higher protein recovery compared with the pH-shift process and using both techniques, recovery ranked the species as herring > salmon > cod. However, the pH-shift process up-concentrated protein from herring and salmon backbones more efficiently than mechanical separation by removing more fat and ash. This consequently reduced n-3 PUFA and vitamin D content in their protein isolates compared with the backbones and mechanically separated meat (MSM). Cod protein isolate, however, contained higher levels of these nutrients compared with MSM. Mechanical separation concentrated vitamins E and C in salmon MSM but not for cod and herring. Opposite, pH-shift processing reduced levels of these two vitamins for cod and herring backbones, while vitamins D and C were reduced for salmon. For minerals, selenium, calcium, magnesium, and potassium were lower in protein isolates than MSM, while copper, zinc, iron and manganese were similar or higher. Overall, there is a major potential for upcycling of fish backbones to food ingredients, but processing technology should be carefully balanced against the desired nutrient profile and final application area.

scholarly journals Optimization of the extraction process of compounds with antioxidant activity of tommy atkins mango peel

2020 ◽  
Vol 9 (10) ◽  
pp. e9939109273
Author(s):  
Felipe da Silva Veloso ◽  
Eliane Colla ◽  
Aziza Kamal Genena
Keyword(s):  
Antioxidant Activity ◽  
Extraction Process ◽  
Fractional Factorial ◽  
Raw Material ◽  
Extraction Time ◽  
Antioxidant Compounds ◽  
Solvent Ratio ◽  
Solution Ph ◽  
Maximum Extraction ◽  
Mango Peel

It is well known that vegetable residues from the food industry can be used as a possible source for the extraction of compounds with antioxidant activity; in the case of mango, approximately 40 to 60% of the total mass of the fruit is considered as residue after processing. This work focused on the optimization of the extraction process of compounds with antioxidant activity from the rind of Tommy Atkins mango. Initially a Fractional Factorial Design 25-1 (FFD) was applied to study the effect of five variables on the extraction process, namely: extraction time (min), percentage of ethanol in aqueous solution (%), pH, dry/solvent mango peel ratio (g/mL) and ultrasound power range (%). The variables extraction time and dry/solvent mango peel ratio were selected, which were evaluated sequentially by the application of a Central Composite Rotatable Design (CCRD) to determine the conditions of maximum extraction of antioxidant compounds by the response surface analysis. The ABTS•+ and Folin-Ciocalteu methods were used for the quantification of the antioxidant activity.  The maximum extraction occurred with the use of water, extraction time of 30 min, dry mango peel/solvent ratio of g/mL (1:100), at natural pH of the mixture (pH 4.6 ± 0.20) and sonication amplitude at 50%. It was possible to develop an extraction process of compounds with antioxidant activity from the mango peel, in order to maximize yield through the use of non-toxic solvents and using an agro-industrial residue as raw material.

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