scholarly journals Comparative Immunomodulatory Efficacy of Rosemary and Fenugreek Against Escherichia Coli-Infection via Suppression of Inflammation and Oxidative Stress in Broilers

2021 ◽  
Author(s):  
Sameh Mohamed Farouk ◽  
Haidy G. Abd-El-Rahman ◽  
Osama A. Abdallah ◽  
Nashwa G. El-Behidy
Keyword(s):  
Body Weight ◽  
Morphological Changes ◽  
Body Weight Gain ◽  
Immunological Response ◽  
Health Condition ◽  
Significant Decline ◽  
Bursa Of Fabricius ◽  
Economic Losses ◽  
Normal Tissues ◽  
Escherichia Coli Infection

Abstract Broilers are frequently infected with Escherichia coli (E.Coli) bacteria, which often leads to the emergence of many diseases and high economic losses. Hence, the present study was performed to assess the comparative efficacy of dietary rosemary and fenugreek, under E.Coli-infection in broilers, via evaluation of growth performance, biochemical indices, immunological response and histo-morphological changes. Eighty Cobb broilers were allotted to four equal groups (n=20 chicks/group); control non-infected (CN), control infected (CI), rosemary infected (RI) and fenugreek infected (FI) groups. RI and FI groups showed a significant elevation in their body weight and body weight gain compared with CI group. Moreover, both groups revealed a significant decline in serum aspartate and alanine aminotransferase activities, as well as uric acid and creatinine levels. Significant decreases of total antioxidant capacity, catalase and superoxide dismutase activities were noted among CI chicks. Moreover, distinctly higher levels were evident in both RI and FI groups. Immunomodulatory markers assessment showed a significant increase in immunoglobulin G with a significant decline in interleukin-6 level in both RI and FI groups, with the lowest IL-6 value within FI group. Histopathological evaluations focused on the deleterious effect associated with E-Coli infection of broilers’ liver, kidney, intestine, spleen, bursa of fabricius, and thymus. A partial histological improvement was noticed among RI group, and nearly normal tissues were recorded in FI group. Overall, these findings suggest the ability of fenugreek to mitigate the adverse effects of E.Coli-infection on broiler performance and tissue pictures, through improvement of the chicken general health condition.

scholarly journals Subchronic Acephate Exposure Induces Immunotoxicity in White Leghorn Cockerels

2021 ◽  
Author(s):  
Syamantak Tripathi ◽  
Prem Govindappa ◽  
Megha Bedekar ◽  
Yash Sahni ◽  
B. Sarkhel ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Body Weight Gain ◽  
Animal Health ◽  
Acetylcholinesterase Activity ◽  
Bursa Of Fabricius ◽  
Delayed Type Hypersensitivity ◽  
White Leghorn ◽  
Groundnut Oil ◽  
Con A ◽  
Live Body Weight

Abstract We investigated the subchronic immunotoxicity of the phosphoramidothioate organophosphorous insecticide, acephate in white leghorn cockerels (WLH). The cockerels were divided into five groups; C1 (plain control), C2 (vehicle control), T1, T2, and T3 which received acephate suspended groundnut oil for 60 days at doses of 21.3, 28.4 and 42.6 mgkg−1respectively. The live body weight gain, absolute and relative weights of the spleen, thymus, and bursa of Fabricius, hemoglobin (Hb), total erythrocyte counts (TEC), packed cell volume (PCV) and lymphocytes were significantly decreased. However, monocytes, eosinophils, heterophils, and basophils were significantly increased. Total protein, albumin and albumin to globin ratio, the antibody response to RD-F and delayed-type hypersensitivity response to DNCB dye or PHA-P, erythrocyte and brain Acetylcholinesterase activity was also significantly reduced in T2 and T3. At 40 and 60 days of acephate exposure, nitrate and nitric oxide production by RD-F and mitogen Con A stimulated peripheral blood and splenic lymphocytes, as well as lymphocyte proliferation in response to antigen RD-F and mitogen Con A stimulation, were significantly decreased in groups T2 and T3. Furthermore, dose-dependent increases in the frequency of micronuclei formation, varying intensity serum protein bands with different protein fractions (14.85KDa), and splenic DNA laddering (180 bp) were observed in groups T2 and T3. Histopathologically, the spleen and bursa showed morphological changes and mild lymphocyte depletion. In conclusion, low-level acephate exposure may affect acetylcholinesterase, lymphocytes, and immune responses in cockerels. As a result, it should be considered when assessing immunotoxicity and the risk to human and animal health.

scholarly journals Subchronic Acephate Exposure Induces Immunotoxicity in White Leghorn Cockerels

2021 ◽  
Author(s):  
Syamantak Mani Tripathi ◽  
Prem Kumar Govindappa ◽  
Megha Kadam Bedekar ◽  
Yash Pal Sahni ◽  
B. C. Sarkhel ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Body Weight Gain ◽  
Animal Health ◽  
Acetylcholinesterase Activity ◽  
Bursa Of Fabricius ◽  
Delayed Type Hypersensitivity ◽  
White Leghorn ◽  
Groundnut Oil ◽  
Con A ◽  
Live Body Weight

Abstract We investigated the subchronic immunotoxicity of the phosphoramidothioate organophosphorous insecticide, acephate in white leghorn cockerels (WLH). The cockerels were divided into five groups; C1 (plain control), C2 (vehicle control), T1, T2, and T3 which received acephate suspended groundnut oil for 60 days at doses of 21.3, 28.4 and 42.6 mgkg− 1respectively. The live body weight gain, absolute and relative weights of the spleen, thymus, and bursa of Fabricius, hemoglobin (Hb), total erythrocyte counts (TEC), packed cell volume (PCV) and lymphocytes were significantly decreased. However, monocytes, eosinophils, heterophils, and basophils were significantly increased. Total protein, albumin and albumin to globin ratio, the antibody response to RD-F and delayed-type hypersensitivity response to DNCB dye or PHA-P, erythrocyte and brain Acetylcholinesterase activity was also significantly reduced in T2 and T3. At 40 and 60 days of acephate exposure, nitrate and nitric oxide production by RD-F and mitogen Con A stimulated peripheral blood and splenic lymphocytes, as well as lymphocyte proliferation in response to antigen RD-F and mitogen Con A stimulation, were significantly decreased in groups T2 and T3. Furthermore, dose-dependent increases in the frequency of micronuclei formation, varying intensity serum protein bands with different protein fractions (14.85KDa), and splenic DNA laddering (180 bp) were observed in groups T2 and T3. Histopathologically, the spleen and bursa showed morphological changes and mild lymphocyte depletion. In conclusion, low-level acephate exposure may affect acetylcholinesterase, lymphocytes, and immune responses in cockerels. As a result, it should be considered when assessing immunotoxicity and the risk to human and animal health.

scholarly journals Anti-aflatoxin B1 effects of Shirazi thyme (Zataria multiflora) in broilers: evaluation of performance and liver histopathology

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Omid Fani Makki ◽  
Arash Omidi ◽  
Hossein Ansari Nik ◽  
Seyed Ahmad Hasheminejad ◽  
Seyed Morteza Hosseini Senjedak
Keyword(s):  
Body Weight ◽  
Aflatoxin B1 ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Bursa Of Fabricius ◽  
Average Weight ◽  
Negative Effects ◽  
Liver Histopathology ◽  
Randomized Design ◽  
Significant Efficacy

An experiment was conducted to study the effect of <em>Zataria multifora</em> (ZM) on the performance and liver histopathology of broiler chickens contaminated with aflatoxin B1 (AFB1). One hundred and sixty Ross 308 male broilers (one-day-old) were divided into four treatment groups with four replicates with 10 birds in each replicate. The chickens were reared on the floor for 35 days. The groups were contaminated with AFB1 at two different concentrations,<em> i.e.,</em> 0 and 1000 ppb, and fed ZM in their feed at the concentrations of 0 and 20 gr Kg 1. The evaluated performance parameters were subjected to a completely randomized design with a 2×2 factorial arrangement of the treatments using SAS software (version 9/1). AFB1 had a statistical lowering effects on the feed intake, body weight, body weight gain and average weight of the carcass, thigh, chest, bursa of fabricius, back and neck. Also, the weights of liver, gizzard, pancreas, proventriculus, abdominal fat, full intestine, and heart were increased with AFB1 (P&lt;0.05). In histopathological evaluations, the liver of chickens that received feed containing AFB1 showed multifocal and varied cytoplasmic vacuolization, severe fatty change, degenerating foci, fibrosis of the portal regions, and bile duct hyperplasia. The variables that were evaluated in this study showed that ZM had significant efficacy in diminishing the aflatoxins negative effects on the chickens.

Experimental inoculation of day-old ducks with Brachyspira pilosicoli and B. alvinipulli

2011 ◽  
Vol 59 (2) ◽  
pp. 165-174 ◽  
Author(s):  
Ákos Thuma ◽  
Ádám Dán ◽  
Éva Kaszanyitzky ◽  
Béla Fazekas ◽  
Ádám Tóth ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Mucous Membrane ◽  
Body Weight Gain ◽  
Clinical Signs ◽  
Experimental Period ◽  
The Body ◽  
Bursa Of Fabricius ◽  
Brachyspira Pilosicoli ◽  
Significant Difference

Two groups of one-day-old Peking ducklings (Groups I and II, 12 birds/group) were inoculated orally withBrachyspira pilosicoliand two groups withB. alvinipulli(Groups III and IV, 12 birds/group). T-2 toxin was added to the feed of Groups II and IV in a dose of 1 mg/kg of feed. Groups V and VI served as uninfected control groups (ducks of Group VI received T-2 toxin). The body weight gain of the ducks was measured and clinical signs were monitored continuously. The birds were sacrificed and necropsied on days 7, 14, 21, and 28 post infection (PI). The liver, spleen, kidney, thymus, bursa of Fabricius, ileum, caecum and colon were examined histologically. Culturing ofBrachyspiraspp. and immunohistochemistry were performed from the sampled parts of the intestines as well. No gross pathological or histological lesions that could be associated withB. pilosicoliorB. alvinipulliwere detectable in the intestinal mucous membrane including the colonised intestinal glands. Mortality did not occur during the experimental period. Decrease in body weight gain was significant in the T-2-toxin-treated groups, and it was slight (not significant) in theBrachyspira-infected groups. Crust on the beaks, necrosis, crusting and ulceration in the mucous membrane of the oral cavity and on the skin of the feet, atrophy of the thymus and bursa of Fabricius due to the effect of T-2 toxin, accompanied by lymphocyte depletion, were observed. These lesions were most prominent on days 14 and 21 PI but were seen on day 28 PI as well. Immunohistochemical detection and reisolation ofB. pilosicoliandB. alvinipulliwere successful on days 7, 14, 21 and 28 days from different segments of the intestine of certain birds, but no significant difference was observed in the colonisation rate between the T-2-toxin-treated and the untreated groups.

scholarly journals Effects of haematinics on body weight and certain haematological values in sheep

1970 ◽  
Vol 2 (2) ◽  
pp. 151-153 ◽  
Author(s):  
ML Sharmin ◽  
M Myenuddin ◽  
MR Amin
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Ferrous Sulphate ◽  
Haemoglobin Concentration ◽  
Health Condition ◽  
The Body ◽  
Haematological Values ◽  
General Health Condition ◽  
Group B

Effects of haematinics on body weight gain and certain haematological values were studied on nine sheep of 1 to 2 years old, divided into three groups (A, B & C), each consisting of 3 sheep during the period from 15 January to 28 February 2002. Sheep of group B were treated with copper sulphate and cobalt sulphate @ 50 mg and 1 mg / head / day respectively and the sheep of group C, in addition to copper and cobalt sulphate, were treated orally with ferrous sulphate @ 200 mg / head / day along with IM injection of Vit-B12 (B50 Forte®, Square) @ 5 ml / sheep every 15 days interval for a period of 45 days whereas group A served as untreated control. Results showed that the body weight gain were significantly (p < 0.05) increased in the haematinics treated groups B and C (12.67 ± 2.33 g and 13.57 ± 2.24 g respectively) in comparison to control (9.37 ± 0.58 g) at 45 days of treatment. Haematological examination showed significantly (p < 0.01) increased haemoglobin (9.33 ± 0.42 g% and 10.0 ± 0.06 g%), packed cell volume (29.0 ± 0.58 % and 30.0 ± 0.58 %), total erythrocyte count (9.67 ± 0.22 and 10.27 ± 0.25 106 / mm3), and mean corpuscular haemoglobin concentration (32.26 ± 0.83 and 32.28 ± 0.53%) in the treated groups B and C at 45 days of treatment respectively. The present findings indicate that the supplementation of haematinics could be used in improving the general health condition in sheep and the haematological values.Key words: Haematinics; body weight; haematological values; Black Bengal goatsdoi: 10.3329/bjvm.v2i2.2559Bangl. J. Vet. Med. (2004). 2 (2): 151-153

scholarly journals Effects of Haematinics on Body Weight and Certain Haemotological Values in Black Bengal Goats

1970 ◽  
Vol 2 (1) ◽  
pp. 55-57
Author(s):  
ML Sharmin ◽  
MA Miah ◽  
M Myenuddin ◽  
M Shahjalal
Keyword(s):  
Body Weight ◽  
Body Weight Gain ◽  
Ferrous Sulphate ◽  
Haemoglobin Concentration ◽  
Health Condition ◽  
The Body ◽  
Corpuscular Haemoglobin ◽  
Haematological Values ◽  
Mean Corpuscular Haemoglobin ◽  
Group B

Effects of haematinics on body weight and certain haematological values were studied in nine female Black Bengal goats of 1 to 2 years old, divided into three groups (A, B, & C), each consisting of 3 goats during the period from 15 January to 28 February 2002. Goats of group B treated orally with copper sulphate and ferrous sulphate @ 50 mg and 200 mg / head / day respectively and the goats of group C, in addition to copper and ferrous sulphate were treated orally with cobalt sulphate @ 1 mg / head / day along with IM injection of Vit-B12 (B50 Forte®, Square) @ 5 ml / goat every 15 days interval for a period of 45 days whereas group A served as untreated control. Results showed that the body weight gain were significantly (p < 0.05) increased in the haematinics treated groups B and C (13.57 ± 3.63 g and 12.83 ± 1.01 g respectively) in comparison to control (13.30 ± 1.40 g) at 45 days of treatment. Haematological examination showed significantly (p < 0.01) increased haemoglobin (10.47 ± 0.13 g% and 10.77 ± 0.12 g%), packed cell volume (31.67 ± 0.33% and 32.33 ± 0.33%), total erythrocyte count (13.90 ± 0.12 and 14.78 ± 0.26 106 / mm3), mean corpuscular volume (22.78 ± 0.12 µ3 and 21.88 ± 0.16 µ3), mean corpuscular haemoglobin (7.53 ± 0.05 and 7.28 ± 0.05 µµg) and mean corpuscular haemoglobin concentration (33.05 ± 0.08 and 33.30 ± 0.09%) in the treated groups B and C at 45 days of treatment respectively. The present findings indicate that the supplementation of haematinics could be used in the improvement of general health condition as well as the haematological parameters. Key words: Haematinics; body weight; haematological values; Black Bengal goats doi: 10.3329/bjvm.v2i1.1936 Bangl. J. Vet. Med. (2004). 2 (1) : 55-57

scholarly journals Evaluation of Cellulosic Polymers and Curcumin to Reduce Aflatoxin B1 Toxic Effects on Performance, Biochemical, and Immunological Parameters of Broiler Chickens

Toxins ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 121 ◽  
Author(s):  
Bruno Solis-Cruz ◽  
Daniel Hernandez-Patlan ◽  
Victor Petrone ◽  
Karine Pontin ◽  
Juan Latorre ◽  
...  
Keyword(s):  
Body Weight ◽  
Aflatoxin B1 ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Skin Pigmentation ◽  
Toxic Effects ◽  
Bursa Of Fabricius ◽  
Immunological Parameters ◽  
Positive Effects ◽  
Cellulosic Polymers

To evaluate the effect of cellulosic polymers (CEL) and curcumin (CUR) on aflatoxin B1 (AFB1) toxic effects on performance, and the biochemical and immunological parameters in broiler chickens, 150 one-day-old male broiler chicks were randomly allocated into five groups with three replicates of 10 chickens per pen: Negative Control (feed); AFB1 (feed + 2 ppm AFB1); CUR (feed + 2 ppm AFB1 + Curcumin 0.2%); CEL (feed + 2 ppm AFB1 + 0.3% Cellulosic polymers); and, CEL + CUR (feed + 2 ppm AFB1 + 0.3% Cellulose polymers + 0.2% Curcumin). Every week, body weight, body weight gain, feed intake, and feed conversion ratio were calculated. On day 21, liver, spleen, bursa of Fabricius, and intestine from five broilers per replicate per group were removed to obtain relative organ weight. Histopathological changes in liver, several biochemical biomarkers, antibody titers, and muscle and skin pigmentation were also recorded. Dietary addition of 0.3% CEL and 0.2% CUR separately significantly diminished some of the toxic effects resulting from AFB1 on performance parameters, relative organs weight, histopathology, immune response, and serum biochemical variables (P < 0.05); however, the combination of CUR and CEL showed a better-integrated approach for the management of poultry health problems that are related with the consumption of AFB1, since they have different mechanisms of action with different positive effects on the responses of broiler chickens.

scholarly journals Comparative analysis of host resistance to Sarcoptes scabiei var. cuniculi in two different rabbit breeds

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Wenrui Wei ◽  
Yongjun Ren ◽  
Nengxing Shen ◽  
Hongyu Song ◽  
Jing Xu ◽  
...  
Keyword(s):  
Body Weight ◽  
Host Resistance ◽  
Body Weight Gain ◽  
Skin Lesions ◽  
Sarcoptes Scabiei ◽  
The Body ◽  
Economic Losses ◽  
Serum Samples ◽  
Domestic Rabbit ◽  
Experimental Infestation

Abstract Background Scabies, caused by infestation of the mite Sarcoptes scabiei, is one of the most severe ectoparasitic diseases in rabbits. Scabies seriously affects the commercial rabbit breeding, causing severe economic losses. Host resistance to S. scabiei is an important factor in further development of the rabbit industry. In the present study, we compared the host resistance to S. scabiei var. cuniculi of a new breed of domestic rabbit propagated by the Sichuan Animal Sciences Academy (QiXing rabbit, QX) compared with that of a traditional rabbit breed in the domestic rabbit industry (IRA rabbit, IRA). Methods Both QX and IRA rabbits were experimentally infested with live S. scabiei var. cuniculi mites for 48 h. Then, during the course of four-week experimental infestation period, the body weight of rabbits was recorded every two weeks for calculating body-weight variations in comparison to the non-infested control rabbits. Skin lesions in the foot area were assessed on weekly basis and serum samples were tested weekly for the estimation of changes in the total antibody levels (IgG, IgE and IgM). Moreover, DNA extracted from the blood samples was amplified for analysis of the genetic diversity in the major histocompatibility complex, class II, DQ Alpha (MHC-DQA) gene. Results Compared to the IRA rabbits, the QX rabbits showed a significantly higher (P < 0.05) relative body weight gain compared to the non-infested control rabbits and significantly lower (P < 0.05) scores for foot skin lesions and higher levels of IgG, IgE and IgM at weeks 1 to 4, week 2 and week 1 post-infestation, respectively. Furthermore, a polymorphism site at position 103 bp of exon two of MHC-DQA gene and a different gene frequency were found between two rabbit breeds, suggesting the genetic basis for the differential host resistance to the S. scabiei var. cuniculi between two rabbit breeds. Conclusions The QX rabbits showed higher host resistance to S. scabiei var. cuniculi compared to the IRA rabbits at the clinical, immunological and genetic levels. These results provide a reference for the breeding of rabbits with adequately improved and sustained host resistance to scabies in the domestic rabbit industry.

Reduction of economic losses caused by mycoplasmal pneumonia of pigs by vaccination with Respisure and by Tiamutin treatment

2003 ◽  
Vol 51 (3) ◽  
pp. 259-271 ◽  
Author(s):  
L. Stipkovits ◽  
Zs. Laky ◽  
T. Abonyi ◽  
Jurate Siugzdaite ◽  
I. Szabó
Keyword(s):  
Body Weight ◽  
Body Weight Gain ◽  
Cost Benefit ◽  
Economic Benefits ◽  
The Body ◽  
Economic Losses ◽  
Control Groups ◽  
Lung Lesions ◽  
Mycoplasmal Pneumonia ◽  
Weaning Piglets

The possibilities and economic benefits of controlling mycoplasmal pneumonia of pigs caused by Mycoplasma hyopneumoniae by immunisation with Respisure and by Tiamutin treatment were studied. The experiment was carried out in a herd comprising 1000 sows which was free of PRRS, Aujeszky's disease, swine dysentery and leptospirosis, and the prevalence of mycoplasmal pneumonia was low because the farm had recently been restocked. Groups C1 and C2 served as untreated controls, while Groups R1 and R2 received a prestarter diet containing 100 ppm Tiamutin from the time of weaning. Piglets of Group R1 were vaccinated with Respisure vaccine once on day 69, while those of Group R2 twice, on days 65 and 80. Piglets of Groups ST1 and ST2 were fed 100 ppm Tiamutin in the diet for 7 days at the time of weaning and then at 4 months of age, while pigs of Group ST2 received such treatment also in the 6th month of life. The efficacy of treatment was analysed on the basis of the number of animals that died, were emergency slaughtered or were retarded in growth in the different groups, the body weight of animals at weaning, at 94 and 148 days of age and at the time of slaughter, their daily body weight gain, the lung lesions found in animals slaughtered from the different groups, the costs of medication and vaccination, and the cost-benefit calculations of the results. The mortality and emergency slaughter rate was 2.88% and 4.62% in Groups ST2 and ST1, respectively, 4.23% and 4.62% in Groups R2 and R1, respectively, and 8.39% and 9.44% in the control groups (C2 and C1, respectively). The rate of growth retardation was 0.48% and 2.12% in Groups R1 and R2, respectively, 1.59% and 3.46% in Groups ST1 and ST2, respectively, as compared to 8.03% and 6.55% in the control groups (C1 and C2, respectively). The severity score of lung lesions was 1.82 and 1.46 in Groups R1 and R2, 2.18 and 2.93 in Groups ST1 and ST2, and 3.83 and 4.02 in the control groups C1 and C2, respectively. The mean finishing weight of pigs was 102.4-107.8 kg and 95.2-106.6 kg in the treated groups and 94.5-98.6 kg in the control groups. The classification of pigs according to the EUROP categories showed a shift to the E and U categories in the treated groups. The average feed cost per one kg of liveweight was 77.89-82.64 Forints in the treated groups and 85.66 Forints in the control groups.

Sign in / Sign up

Export Citation Format

Share Document