scholarly journals Anti-aflatoxin B1 effects of Shirazi thyme (Zataria multiflora) in broilers: evaluation of performance and liver histopathology

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Omid Fani Makki ◽  
Arash Omidi ◽  
Hossein Ansari Nik ◽  
Seyed Ahmad Hasheminejad ◽  
Seyed Morteza Hosseini Senjedak

An experiment was conducted to study the effect of <em>Zataria multifora</em> (ZM) on the performance and liver histopathology of broiler chickens contaminated with aflatoxin B1 (AFB1). One hundred and sixty Ross 308 male broilers (one-day-old) were divided into four treatment groups with four replicates with 10 birds in each replicate. The chickens were reared on the floor for 35 days. The groups were contaminated with AFB1 at two different concentrations,<em> i.e.,</em> 0 and 1000 ppb, and fed ZM in their feed at the concentrations of 0 and 20 gr Kg 1. The evaluated performance parameters were subjected to a completely randomized design with a 2×2 factorial arrangement of the treatments using SAS software (version 9/1). AFB1 had a statistical lowering effects on the feed intake, body weight, body weight gain and average weight of the carcass, thigh, chest, bursa of fabricius, back and neck. Also, the weights of liver, gizzard, pancreas, proventriculus, abdominal fat, full intestine, and heart were increased with AFB1 (P&lt;0.05). In histopathological evaluations, the liver of chickens that received feed containing AFB1 showed multifocal and varied cytoplasmic vacuolization, severe fatty change, degenerating foci, fibrosis of the portal regions, and bile duct hyperplasia. The variables that were evaluated in this study showed that ZM had significant efficacy in diminishing the aflatoxins negative effects on the chickens.

Toxins ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 121 ◽  
Author(s):  
Bruno Solis-Cruz ◽  
Daniel Hernandez-Patlan ◽  
Victor Petrone ◽  
Karine Pontin ◽  
Juan Latorre ◽  
...  

To evaluate the effect of cellulosic polymers (CEL) and curcumin (CUR) on aflatoxin B1 (AFB1) toxic effects on performance, and the biochemical and immunological parameters in broiler chickens, 150 one-day-old male broiler chicks were randomly allocated into five groups with three replicates of 10 chickens per pen: Negative Control (feed); AFB1 (feed + 2 ppm AFB1); CUR (feed + 2 ppm AFB1 + Curcumin 0.2%); CEL (feed + 2 ppm AFB1 + 0.3% Cellulosic polymers); and, CEL + CUR (feed + 2 ppm AFB1 + 0.3% Cellulose polymers + 0.2% Curcumin). Every week, body weight, body weight gain, feed intake, and feed conversion ratio were calculated. On day 21, liver, spleen, bursa of Fabricius, and intestine from five broilers per replicate per group were removed to obtain relative organ weight. Histopathological changes in liver, several biochemical biomarkers, antibody titers, and muscle and skin pigmentation were also recorded. Dietary addition of 0.3% CEL and 0.2% CUR separately significantly diminished some of the toxic effects resulting from AFB1 on performance parameters, relative organs weight, histopathology, immune response, and serum biochemical variables (P < 0.05); however, the combination of CUR and CEL showed a better-integrated approach for the management of poultry health problems that are related with the consumption of AFB1, since they have different mechanisms of action with different positive effects on the responses of broiler chickens.


2020 ◽  
Vol 20 (2) ◽  
Author(s):  
Zulfan Zulfan ◽  
Zulfikar Zulfikar

ABSTRAK. Penelitian ini bertujuan untuk mengevaluasi performa tiga strain ayam broiler yang umumnya dipelihara di Aceh. Materi yang digunakan adalah 150 ekor anak ayam broiler (DOC) strain Cobb 500, CP 707, dan MB 202 masing-masing berjumlah 50 ekor. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan dan 5 ulangan. Setiap ulangan merupakan unit percobaan yang masing-masing terdiri dari 10 ekor anak ayam. Perlakuan adalah tiga strain ayam broiler: Cobb 500 (P1), CP 707 (P2), dan MB 202 (P3). Parameter yang diamati meliputi pertambahan berat badan, konsumsi ransum, konversi ransum (FCR), mortalitas, dan Income over Feed and Chick Cost (IOFCC). Hasil penelitian memperlihatkan performa broiler dipengaruhi oleh jenis strain. Strain MB 202 dan CP 707 memiliki bobot badan akhir (6 minggu) dan konsumsi ransum nyata (P0,05) lebih tinggi daripada Cobb 500. Angka konversi ransum tidak memperlihatkan perbedaan yang nyata antara ketiga strain pada semua umur panen. Nilai IOFCC tertinggi dipengaruhi oleh jenis strain dan lama pemeliharaan. Dari hasil penelitian dapat disimpulkan bahwa performa broiler dipengaruhi oleh kemampuan adaptasi strain terhadap iklim setempat (pesisir Aceh) pada periode berbeda-beda yang dapat memengaruhi tingkat keuntungannya. Strain Cobb 500 memiliki kemampuan adaptasi lebih baik selama periode awal, sedangkan CP 707 dan MB 202 selama periode akhir. (Evaluation of performances and income over feed chick cost (iofcc) of three broiler chicken strains commonly marketed in Aceh) ABSTRACT. The study aimed to evaluate the performances and Income over Feed and Chick Cost (IOFCC) of three broiler chicken strains commonly marketed in Aceh. As many as 150 chicks of three different broiler strains (50 birds each) were used in this study. The study was performed into Completely Randomized Design (CRD) consists of 3 treatments and 5 replications creating 15 experimental units containing 10 birds each. The treatments were 3 different strains of broiler chickens i.e. Cobb 500, CP 707, and MB 202. The birds were reared up to 6 weeks to expose their performances recorded as final body weight, body weight gain, feed intake, and feed conversion, as well as mortality. Economic value was evaluated by calculating IOFCC. Results of study showed that performances of broilers were significantly (P0,05) influenced by the strains. Strains MB 202 and CP 707 represented more superior than Cobb 500 for 5−6 weeks of raising periods. However, the later was not inferior during initial phase close to 4 weeks. There were no significant differences in FCR among all strains for different ages. The IOFCC related to the strains and their marketing ages. The highest IOFCC was obtained by Cobb 500, CP 707, and MB 202 as they were sold at the ages of 4, 5, and 6 weeks, respectively. In conclusion, the achievements of the performances in various broiler strains were affected by the site climate (Aceh) in different stages of their ages. Cobb 500 had proper adaptation during starter, while CP 707 and MB 202 appeared seem to be better than Cobb 500 during finisher period.


2020 ◽  
Vol 40 (1) ◽  
pp. 79-82
Author(s):  
C. T. Ezeokeke

Experiment was carried out to determine performance of day old local fowls, fed supplemented plantain peels meal (PPM). The birds were arranged in completely randomized design having six birds per replicate and two treatments comprising one test diet and one control diet. At starter phase, all the parameters (body weight, body weight gain, feed intake and feed conversion ratio) measured were not significant (p>0.05). The birds on the test diet had average initial weight, 42.00g while that of the control had average weight, 43.75g and at 5 weeks of age average weights of 133.30g and 134.54g (p>0.05) ,respectively. At the finisher phase, all the parameters measured were not significant (p>0.5). The prices per kg of feed for the test and control diets were N93.05 and N95.05, respectively. Therefore, PPM inclusion fed at early stage of life of the birds enhanced performance at a lower cost.


2016 ◽  
Vol 40 (3) ◽  
pp. 170
Author(s):  
Eny Sholikhatin ◽  
Ferry Poernama ◽  
Nanung Danar Dono ◽  
Zuprizal .

The aim of this study was to determine the calcium requirements of broiler chickens in starter phase with the addition of phytase enzyme. This experiment used 1,200 broiler chickens (mixed sex) for 21 days rearing period in a closed house. The basal diets was corn and soybean meal which contained 0.097% Ca and 0.123% P-av. The treatments consisted of 6 diets treatment: P1 (basal diet with the addition of 0.42% non-phytate phosphorus (NPP), and then P2 to P6 were basal diet with the addition of 0.22% NPP and 1,000 FTU/kg phytase and the addition of calcium (Ca) at different levels: 0.82% (P2); 0.74% (P3); 0.66% (P4); 0.58% (P5) and 0.50% (P6). The growth performance, feed efficiency, and bone mineralization parameters were studied using Oneway ANOVA in a Completely Randomized Design. Duncan's new Multiple Ranges Test was used to separate means with significant differences. Results showed that 0.90% Ca supplementation without phytase and the reduction levels of Ca from 0.82% to 0.50% increased the amount of feed consumed by birds in all growth phases (P<0.05). The addition of phytase had beneficial effects on increasing body weight (P<0.05) and average of body weight gain (P<0.05), and decreased the value of FCR in 11 - 21 days and 1 - 21 days. The addition of phytase increased protein and energy consumption, followed by increased in the value of PER and  EER (P<0.05). When phytase was added in the diets, reduction levels of Ca in the diets up to 0.50% did not give any adverse effect on the tibia bone ash. It can be concluded that feeding with phytase can sustain growth performance, feed efficiency, and bone mineralization.


2012 ◽  
Vol 12 (2) ◽  
pp. 37-41
Author(s):  
Meisji Liana Sari ◽  
F. Gurki N Ginting

The effect of supplementation phytase enzyme into poultry feed on the relative weight of broiler's digestive organs ABSTRACT. The objective of this research was to know the effect of enzim phytase supplementation on relative organ digestion broiler. The research was done for weeks at Animal Fees and Nutrition, Faculty of Agriculture, Sriwijaya University in Mei to Juni 2008 utilized. A Completely Randomized Design with 6 treatments {P0 (100% basal diet), P1 (100% basal diet + 500 FTU/kg), P2 (100% basal diet + 600 FTU/kg), P3 (100% basal diet + 700 FTU/kg), P4 (100% basal diet + 800 FTU/kg),P5 (100% basal diet + 900 FTU/kg)}. Each treatments was replicated for 3 times. The parameters are final body weight, (%) proventrikulus, (%) relatif intestine, (%) pancreas and (%) liver weigh broiler chickens . The research showed that adding enzim phytase has join no significant effect (P0.05) on the body, liver, proventrikulus, ventrikulus weight, bur it showed significant effect on relative intestines and pancreas weight (P0.05) of broiler chickens . the average weight of body weight was 1117.50-1873.00 gram, proventrikulus was 0,44-0,64% ventrikulus was 1.30-2.32%, intestine was 2.74-4.39%, pancreas was 0,21-0,32% and liver was 2,35-3.01%.


PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0254462
Author(s):  
Bahadır Can Güz ◽  
Ingrid C. de Jong ◽  
Carol Souza Da Silva ◽  
Fleur Veldkamp ◽  
Bas Kemp ◽  
...  

Pen enrichment for broiler chickens is one of the potential strategies to stimulate locomotion and consequently contribute to better leg health and welfare. This study was designed to evaluate effects of using a plethora of pen enrichments (barrier perches, angular ramps, horizontal platforms, large distance between feed and water and providing live Black Soldier fly larvae in a dustbathing area) on tibia characteristics, locomotion, leg health and home pen behaviour of fast and slower-growing broiler chickens. The experiment was set up as a 2 x 2 factorial arrangement with a total of 840 male broiler chickens in a complete randomized design (7 pens per treatment and 30 chickens per pen) with the following treatments: 1) pen enrichment (enriched pen or non-enriched pen); 2) broiler strain (fast-growing Ross 308 or slower-growing Hubbard JA 757). Home pen behaviour and use of enrichment were observed. At approximately 1400 and 2200 g body weight, two chickens per pen were randomly selected and slaughtered, to investigate tibia morphological, biophysical and mechanical characteristics and leg health. Pen enrichment positively affected tibia biophysical characteristics, e.g., osseous volume (Δ = 1.8 cm3, P = 0.003), total volume (Δ = 1.4 cm3, P = 0.03) and volume fraction (Δ = 0.02%, P = 0.002), in both fast and slower-growing chickens, suggesting that pen enrichment particularly affects ossification and mineralization mechanisms. Accordingly, locomotion and active behaviours were positively influenced by pen enrichment. However, pen enrichment resulted in lower body weight gain in both strains, which might be due to higher activity or lower feed intake as a result of difficulties of crossing the barrier perches. Regarding the strain, slower-growing chickens showed consistently more advanced tibia characteristics and more active behaviour than fast-growing chickens. It can be concluded that pen enrichment may lead to more activity and better bone development in both fast and slower-growing chickens.


2019 ◽  
Vol 20 (3) ◽  
pp. 352
Author(s):  
Bambang Sulistiyanto ◽  
Sri Kismiati ◽  
Cahya Setya Utama

An immunomodulator is a natural body defence mechanism due to the stimulation of objects/ environments, both specific and non-specific by means of cellular and humoraldefence. The objective of the study was to prove the immunomodulation ability of broiler chickens up to age 35 days that dietary fed various wheat pollard based rations. The design used in the study was a complete randomized design with six treatments and three replications. The treatment of broiler rats consisted of BR-IAJ (T0), control feed with wheat pollard base (T1), control feed with wheat pollard base plus probiotic (T2), wheat pollard based feed steamed (T3), fermented wheat pollard based feed 40% (T4 ) and fermented wheat pollard based feed 60% (T5). The rations used contained 20,5-22,5% protein with 2900-3100 Kcal metabolic energy. Lohman MB 202 Platinum from PT. Japfa Comfeed used in research with average day old chick (DOC) weight of 36,39 + 2,45 g. Parameters observed included body weight, feed intake, feed conversion ratio (FCR), relative weights of liver organ, thymus, lymph and bursa fabricius. The results showed that wheat pollard-based rations had a significant effect (p <0,05) on the relative weight of liver organ, body weight, FCR and ration consumption, but did not affect the relative weights of spleen organ, thymus and bursa fabricius. The mean relative weight of consecutive liver as follows: 2,47 g (T0), 2,83 g (T1), 3,40 g (T2), 2,99 g (T3), 3,15 g (T4) and 3,06 g (T5). The average body weight, consumption and FCR rations were as follows: 1415,24 g; 136,45 g/day; 2,03 (T0), 775,04 g; 125,19 g/day; 3,40 (T1), 813,17 g; 129,86 g / day; 3,36 (T2), 792,89 g; 128,14 g /day; 3,54 (T3), 892,91 g; 138,33 g /day; 3,27 (T4), 969,56 g; 155,52 g/day; 3,37 (T5). The conclusion of the study was that the ration with the addition of wheat pollard 60% was able to provide an increase in body weight gain and the best immunomodulation seen from the aspect of the proportion of immunomodulating organs to the growth of broiler chickens.


Gut Pathogens ◽  
2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Daniel Ruhnau ◽  
Claudia Hess ◽  
Barbara Doupovec ◽  
Bertrand Grenier ◽  
Dian Schatzmayr ◽  
...  

Abstract Background Intestinal epithelial cells are challenged by mycotoxins and many bacterial pathogens. It was previously shown that the mycotoxin deoxynivalenol (DON) as well as Campylobacter (C.) jejuni have a negative impact on gut integrity. Recently, it was demonstrated that DON increased the load of C. jejuni in the gut and inner organs. Based on this finding, it was hypothesized the DON metabolite (deepoxy-deoxynivalenol, DOM-1) should be able to reduce the negative effects of DON on colonization and translocation of C. jejuni in broilers, since it lacks the epoxide ring, which is responsible for the toxicity of DON. Methods A total of 180 broiler chickens were housed in floor pens on wood shavings with feed and water provided ad libitum. Birds were divided into six groups (n = 30 with 5 replicates/group): 1. Control, 2. DOM-1, 3. DON, 4. DOM-1 + C. jejuni, 5. DON + C. jejuni, 6. C. jejuni. At day 14, birds of groups 4, 5 and 6 were orally inoculated via feeding tube (gavage) with 1-ml of a PBS suspension containing 1 × 108 CFU of C. jejuni NCTC 12744. The performance parameters: body weight (BW), body weight gain (BWG), and feed intake of the birds were determined. At 7, 14, and 21 days post infection, samples from liver, spleen, duodenum, jejunum and cecum were aseptically collected and processed for bacteriological investigations. Finally, at each killing time point, segments of duodenum, jejunum and cecum were harvested and prepared for Ussing chamber studies to measure the paracellular mannitol fluxes. Results A significant decrease in body weight was observed for chickens receiving the DON diet with or without C. jejuni compared to the other groups. Furthermore, it was found that the co-exposure of birds to DON and C. jejuni resulted in a higher C. jejuni load not only in the gut but also in liver and spleen due to increased paracellular permeability of the duodenum, jejunum and cecum. On the contrary, DOM-1 supplementation in the feed improved the birds’ performance and led to a better feed conversion ratio throughout the trial. Furthermore, DOM-1 did not negatively affect gut permeability and decreased the C. jejuni counts in the intestine and internal organs. Conclusion Altogether, the presence of DOM-1 in the feed as a result of the enzymatic biotransformation of DON leads to a lower C. jejuni count in the intestine and better feed conversion ratio. Moreover, this study demonstrates that the detoxification product of DON, DOM-1, does not have negative effects on the gastrointestinal tract and reduces the Campylobacter burden in chickens and also the risk for human infection.


2016 ◽  
Vol 3 (1) ◽  
pp. 29-37
Author(s):  
. Andriyanto ◽  
Aryani Sismin Satyaningtijas ◽  
Raden Yufiandri ◽  
Regina Wulandari ◽  
Vinda Mulyetti Darwin ◽  
...  

Testosteron merupakan salah satu hormon anabolik yang dapat memacu pertumbuhan massa otot dan tulang. Hormon testosteron mampu merangsang sekresi growth hormone yang berperan dalam pertumbuhan. Penelitian ini bertujuan untuk mempelajari pengaruh pemberian hormon testosteron dengan dosis bertingkat terhadap performa dan kecernaan pakan ayam broiler. Sebanyak 44 ekor ayam broiler berumur 15 hari dengan rata-rata bobot badan antara 650 ± 71 g dibagi menjadi 4 kelompok (K, T1, T2, dan T3). K adalah kelompok kontrol; T1, T2, dan T3 adalah kelompok yang diberi testosteron dosis 1, 2, dan 4 mg per ekor. Parameter yang diukur adalah bobot badan, konsumsi pakan, rasio konversi pakan, serta persentase kandungan nutrisi (di dalam pakan dan feses). Hasil penelitian menunjukkan pemberian testosteron dosis 1 dan 4 mg dapat meningkatkan pertambahan bobot badan harian secara signifikan (p<0,05) pada ayam broiler yang berumur antara 15 dan 18 hari. Testosteron dosis 2 mg meningkatkan bobot badan harian secara signifikan (p<0,05) pada ayam broiler yang berumur antara 21 dan 24 hari. Konsumsi pakan, rasio konversi pakan, dan kecernaan pakan ayam broiler yang diberi hormon testosteron tidak menunjukkan perbedaan dibanding dengan kontrol. Akan tetapi, pada kelompok yang diberi testosteron dosis 4 mg, nilai kecernaan lemak, protein, dan karbohidrat cenderung meningkat.Kata kunci: broiler, kecernaan, pertambahan bobot badan harian, proksimat, testosteron (Broiler Chickens Performance and Feed Digestibility Treated with Multi-Dose Testosterone Hormone)Testosterone is one of the anabolic hormone that can trigger the growth of muscle mass and bone. Testosterone hormone can stimulate secretion of growth hormone that has a role in growth. The aim of this research was to observe the administration of multi-dose testosterone hormone on broiler chickens performance and feed digestibility. Fourty four broiler chickens 15 days old with average weight 650 ± 71 g were divided into 4 groups (K, T1, T2, and T3). K was control group; T1, T2, and T3 were groups which given testosterone dose 1, 2, and 4 mg each chicken. Parameters measured were body weight, feed consumption, feed conversion ratio (FCR), and nutritions percentage (in feed and feces). The results showed that testosterone dose 1 and 4 mg could increase the daily body weight gain significantly (p<0.05) in broiler chickens aged between 15 and 18 days old. Testosterone dose 2 mg could increase the daily body weight gain significantly (p<0.05) in broiler chickens aged between 21 and 24 days old. Feed consumption, FCR, and feed digestibility of chickens given testosterone did not show any difference compare with control. However, in group which given testosterone dose 4 mg, the digestibility values of fat, protein, and carbohydrates tended to increase.Keywords: broiler, digestibility, daily body weight gain, proximate, testosterone


Cassowary ◽  
2018 ◽  
Vol 1 (1) ◽  
pp. 55-62
Author(s):  
Alimuddin Alimuddin ◽  
M. Jen Wajo ◽  
Marlyn N Lekitoo

The research was aimed to study Bali bulls fed with Pennisetum purpureum with fermented rice straws substitution in Manokwari Regency, West Papua was conducted experimentally with 12 Bali bulls that have average weight of 176.02±38.35 kg with 2 – 2,5 years old for 5 months duration. The study was designed using complete randomized design of nested pattern with 3 (three) treatments, 4 (four) replications and 4 (four) times weighing, with an assumption that Bali bulls are nested during weighing time. The results of this study showed that Penicetum purpureum substitution with fermented straws did not significant on Bali bulls body weight. The duration of fermented rice straws feeding that substituted with Penicetum purpureum significant for Bali bulls weight gain with the average absolute weight gain is 0,68 kg/ day, as well as relative body weight gain of 0,033 kg/ day. The potential of rice straws in Manokwari Regency might be utilized as Bali bulls feed as many 9.431 – 46.846 cattle.


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