scholarly journals In Vitro Screening of Cucmis Melo L. Against Drought Mediated By PEG and Sorbitol

2021 ◽  
Author(s):  
Maryam Nekoee Mehmandar ◽  
Farzad Rasouli ◽  
Mousa Torabi Giglou ◽  
Seyed Morteza Zahedi ◽  
Mohammad Ali Aazami
Keyword(s):  
Antioxidant Enzymes ◽  
Water Deficit ◽  
Photosynthetic Pigments ◽  
Dry Weight ◽  
Growth Stages ◽  
Guaiacol Peroxidase ◽  
Coleoptile Length ◽  
Screening And Identification ◽  
Osmotic Solution

Abstract Water deficit in first growth stages of melon (Cucumis melo L.) in formation of first true leaves after germination can be a factor limiting production. The first step for resolve the problem is genotypes evaluation and identification of drought tolerant melons. An effective method to achieve the goal is use of osmotic solution in tissue culture. Responses of Iranian melon landraces to drought was evaluated using sorbitol at 0.1, 0.2 and 0.4 M or polyethyleneglycol (PEG 6000) at 0.009, 0.012 and 0.015 M concentrations, and MS medium without treatment as the control. Coleoptile length, fresh and dry weight of shoots and roots, photosynthetic pigments, protein, proline, malondialdehyde (MDA) and antioxidant enzymes superoxide dismutase, guaiacol peroxidase and ascorbate peroxidase were measured. The PEG or sorbitol decreased coleoptile length, fresh weight and photosynthetic pigments, and led to enhancement of proline and MDA. Contents of protein and antioxidant enzymes was completely dependent on genotype and type and concentration of osmotic material. The in vitro culture for screening and identification of tolerant and sensitive drought genotypes could be rapid, useful and effective, with sorbitol mimicing drought better than PEG. After in vitro evaluation, the genotype responses to induced water deficit need to be confirmed under field conditions.

scholarly journals Responses of antioxidant enzymes, photosynthetic pigments and carbohydrates in micropropagated Pitcairnia encholirioides L.B. Sm. (Bromeliaceae) under ex vitro water deficit and after rehydration

2019 ◽  
Vol 79 (1) ◽  
pp. 53-62 ◽  
Author(s):  
C. F. Resende ◽  
V. S. Pacheco ◽  
F. F. Dornellas ◽  
A. M. S. Oliveira ◽  
J. C. E. Freitas ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Water Deficit ◽  
Photosynthetic Pigments ◽  
Culture Conditions ◽  
Proline Content ◽  
High Tolerance ◽  
Ex Vitro ◽  
Carbohydrate Contents ◽  
Progressive Water Stress

Abstract In this study, the activities of antioxidant enzymes, photosynthetic pigments, proline and carbohydrate contents in Pitcairnia encholirioides under ex vitro conditions of water deficit were evaluated. Results show that plants under progressive water stress, previously in vitro cultured in media supplemented with 30 g L-1 sucrose and GA3, accumulated more proline and increased peroxidase (POD) activity and the contents of photosynthetic pigments and carbohydrates. For plants previously in vitro cultured with 15 g L-1 sucrose and NAA, no differences were found for proline content and there were reductions in activities of peroxidase (POD), catalase (CAT) and poliphenoloxidase (PPO), and in contents of carbohydrates, with progress of ex vitro water deficit. After rehydration, plants showed physiological recovery, with enzymatic activities and contents of metabolites similar to those found in the controls not submitted to dehydration, regardless of the previous in vitro culture conditions. These results show that micropropagated P. encholirioides has high tolerance to dehydration once in ex vitro conditions, which can ensure the survival of plants from tissue culture when transferred to its natural environment, emphasizing the importance of such biotechnology for the propagation of endangered species.

scholarly journals Silver Nanoparticles Effects on In Vitro Germination, Growth, and Biochemical Activity of Tomato, Radish, and Kale Seedlings

Materials ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5340
Author(s):  
Alicja Tymoszuk
Keyword(s):  
Silver Nanoparticles ◽  
Practical Importance ◽  
Plant Cells ◽  
Dry Weight ◽  
Guaiacol Peroxidase ◽  
In Vitro Germination ◽  
Vegetable Crops ◽  
Fresh Weight ◽  
Biochemical Activity

The interactions between nanoparticles and plant cells are still not sufficiently understood, and studies related to this subject are of scientific and practical importance. Silver nanoparticles (AgNPs) are one of the most commonly produced and used nanomaterials. This study aimed to investigate the influence of AgNPs applied at the concentrations of 0, 50, and 100 mg·L−1 during the process of in vitro germination as well as the biometric and biochemical parameters of developed seedlings in three vegetable species: Solanum lycopersicum L. ‘Poranek’, Raphanus sativus L. var. sativus ‘Ramona’, and Brassica oleracea var. sabellica ‘Nero di Toscana’. The application of AgNPs did not affect the germination efficiency; however, diverse results were reported for the growth and biochemical activity of the seedlings, depending on the species tested and the AgNPs concentration. Tomato seedlings treated with nanoparticles, particularly at 100 mg·L−1, had shorter shoots with lower fresh and dry weights and produced roots with lower fresh weight. Simultaneously, at the biochemical level, a decrease in the content of chlorophylls and carotenoids and an increase in the anthocyanins content and guaiacol peroxidase (GPOX) activity were reported. AgNPs-treated radish plants had shorter shoots of higher fresh and dry weight and longer roots with lower fresh weight. Treatment with 50 mg·L−1 and 100 mg·L−1 resulted in the highest and lowest accumulation of chlorophylls and carotenoids in the leaves, respectively; however, seedlings treated with 100 mg·L−1 produced less anthocyanins and polyphenols and exhibited lower GPOX activity. In kale, AgNPs-derived seedlings had a lower content of chlorophylls, carotenoids, and anthocyanins but higher GPOX activity of and were characterized by higher fresh and dry shoot weights and higher heterogeneous biometric parameters of the roots. The results of these experiments may be of great significance for broadening the scope of knowledge on the influence of AgNPs on plant cells and the micropropagation of the vegetable species. Future studies should be aimed at testing lower or even higher concentrations of AgNPs and other NPs and to evaluate the genetic stability of NPs-treated vegetable crops and their yielding efficiency.

scholarly journals Effect Of Priming Of Seeds Of Medicago Sativa ‘Bami’ With Gibberellic Acid On Germination, Seedlings Growth And Antioxidant Enzymes Activity Under Salinity Stress

2014 ◽  
Vol 22 (2) ◽  
pp. 167-174 ◽  
Author(s):  
Omid Younesi ◽  
Ali Moradi
Keyword(s):  
Antioxidant Enzymes ◽  
Gibberellic Acid ◽  
Salinity Stress ◽  
Reductase Activity ◽  
Membrane Damage ◽  
Dry Weight ◽  
Guaiacol Peroxidase ◽  
Enzymes Activity ◽  
Mda Content ◽  
Glutathione Reductase Activity

AbstractThe experiment was conducted in order to study effects of seeds priming with gibberellic acid (GA3) at 0, 3, 5 and 8 mM on germination, growth and antioxidant enzymes activity in alfalfa seedlings under salinity stress (200 mM NaCl). All control seeds germinated. The rate of germinated seeds was reduced to 48% in the presence of NaCl, and increased to 76% after seeds priming with 5 mM GA3. Priming with 5 mM GA3 was also correlated with an increase of dry weight of seedlings derived from both stressed and non-stressed seeds as well as with the reduction of electrolyte leakage (EL) and malondialdehyde (MDA) level in salt stressed seedlings. The activity of superoxide dismutase, catalase, guaiacol peroxidase and ascorbate peroxidase in primed and non-primed seeds increased in the presence of NaCl and after priming of seeds with 5 mM GA3, whereas only small effect on glutathione reductase activity in both primed and non-primed seeds was observed. The total ascorbate level was higher in both stressed and non-stressed seedlings from primed seeds. These results suggest that GA3 priming might increase the salt tolerance of alfalfa seedlings through enhancing the activities of antioxidant enzymes and reducing the membrane damage as estimated using biomarkers, EL index and MDA content.

scholarly journals Biohardening of Arabidopsis thaliana Seeds and Seedlings with Fraser Photinia Associated Bacterium (PGB_invit) in In vitro Conditions

2019 ◽  
Vol 47 (3) ◽  
pp. 954-961
Author(s):  
Maria BATOOL ◽  
Özlem AKKAYA ◽  
Mine GÜL ŞEKER ◽  
Yelda ÖZDEN ÇİFTÇİ
Keyword(s):  
Arabidopsis Thaliana ◽  
Photosynthetic Pigments ◽  
Growth Parameters ◽  
Endophytic Bacterium ◽  
Dry Weight ◽  
Bacterial Populations ◽  
Positive Effects ◽  
Inactive Form ◽  
Potential Possibility

The aim of this study was to analyze possible positive effects of putatively endophytic PGPB (PGB_invit), which was isolated from long-term in vitro cultured fraser photinia microshoots, on seed and 7-day old seedling stages of Arabidopsis thaliana. Seeds and in vitro-germinated seedlings were inoculated with 107 CFU/mL and 108 CFU/mL active (A) and inactive (I) endophytic bacterial populations along with their mix compositions (A+I) and suspended in MPYE broth together with their controls (untreated ones). 14 days old seedlings were evaluated for various plant growth parameters [i.e., shoot and root fresh weight (FW), shoot length (SL), shoot and root dry weight (DW), root length (RL) and photosynthetic pigments including chlorophyll a, b and carotenoids of plantlets] as well as endophytic and rhizospheric trait of bacteria. Positive effects of inactive and active bacterium on FW, DW and photosynthetic pigments for 7-day inoculated seedlings were recorded whereas an increase in photosynthetic contents for seed stage inoculations was observed. Rhizospheric and endophytic colonization of the bacteria was confirmed by PCR with the presence of virD1 gene, which is previously recorded to be existed in the plasmid of bacterium after inoculation. Overall, these results demonstrated that this peculiar putative endophytic bacterium being beneficial in active and even more useful in inactive form for A. thaliana when optimum conditions and concentrations are used. Moreover, presence of virD1 gene suggested its potential possibility to be used in bioengineering along with various other beneficial PGPR features as biofertilizer.

scholarly journals Inhibitory impact of Daraikh (Melia Azedarach) leaves litter on wheat (Triticum aestivum) seedling

2021 ◽  
Vol 27 (2) ◽  
pp. 191-200
Author(s):  
Iqtidar Hussain
Keyword(s):  
Triticum Aestivum ◽  
Negative Impact ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Inhibitory Effect ◽  
Germination Percentage ◽  
Melia Azedarach ◽  
Growth Stages ◽  
Initial Growth ◽  
Coleoptile Length

A biological phenomenon by which one plant releases some chemicals in the environment that affect the rate of germination, its seedling emergence and physiology and overall growth of neighboring plants is called allelopathy. The significance of study was checked allelopathic phytochemical potential of Daraikh (Melia Azedarach L.) leaves on Wheat. Leaves litter were used to examine the allelopathic effects of Daraikh (Melia Azedarach) at five concentrations (100, 200, 300, 400 g, control) parameters studied germination percentage (%), Speed of germination, plant height (cm), root length (cm), Shoot length (cm), coleoptile length (cm), Fresh weight (g) and dry weight (g), Tiller (plant-1) and chlorophyll content (µ cm-2) of Triticum aestivum. All concentration of Leaves litter of Melia Azedarach showed pronounced inhibitory effect on all parameters of Triticum aestivum. Melia Azedarach exerted phytotoxic influence on Triticum aestivum at initial growth stages. Melia Azedarach exhibited a significant negative impact on germination of Triticum aestivum at 100, 200, 300, 400 g litter of leaves than control (Sterilized soil) repectively. Melia Azedarach halted the coleoptile length of Triticum aestivum @ 400 g leaves litter. Powdered leaves of Melia Azedarach in clay loam soil appeared to have strong allelopathic inhibition under maximum concentrations on growth and germination of Triticum aestivum. Hence, Melia Azedarach proved a strong allelopathic plant that should be planted aside from field to avoid harmful impacts during early growth stages of Triticum aestivum.

scholarly journals Biochemical Response and Nutrient Uptake of Two Arbuscular Mycorrhiza-Inoculated Chamomile Varieties Under Different Water Potentials

2021 ◽  
Author(s):  
Fatemeh Ebrahimi ◽  
Amin Salehi ◽  
Mohsen Movahedi Dehnavi ◽  
Amin Mirshekari ◽  
Mohammad Hamidian ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Arbuscular Mycorrhiza ◽  
Water Deficit ◽  
Crop Production ◽  
Soluble Sugars ◽  
Dry Weight ◽  
Water Deficit Stress ◽  
Water Potentials ◽  
Randomized Design ◽  
Total Dry Weight

Abstract BackgroundWater-deficit stress is one of the most important sources of damage to crop production worldwide. Adopting appropriate varieties using soil microorganisms such as arbuscular mycorrhiza(AM) can significantly reduce theadverseeffectsofwater deficiency.This study is aimed to evaluate the role of Funneliformismosseaeon nutrients uptake and some physiological traits of two chamomile varieties namely Bodgold (Bod) and Soroksári(Sor) under water-deficit stress. The pot experiment was performed in a hydroponic system within a completely randomized design considering four replications. Three levels of water-deficit stress (PEG 6000) were taken into account at water potentials of -0.4 and -0.8MPa. The second factor was AM inoculation.ResultsWater-deficit stress significantly reduced the uptake of macro-nutrients (N, P, and K) and micro-nutrients (Fe, Cu, Mn, and Zn) in the shoots and roots. Moreover, the level of osmolytes (total soluble sugars and proline) and the activity of antioxidant enzymes in the shoots of both varieties increased under water-deficit stress. In the case of Sor variety, the level of these compounds was more satisfactory. AM improved plant nutrition uptake and osmolyte contents while enhancing antioxidant enzymes and reducing theadverseeffectsofwater-deficit stress. Under water-deficit stress, the growth and total dry weight improved upon AM inoculation. ConclusionsIn general, inoculation of chamomile with AM balanced the uptake of nutrients increased the level of osmolytes, antioxidant enzymes, and hence improved plant characteristics under water-deficit stress in both varieties, however, it was more effective in reducing stress damages in Sor variety.

scholarly journals Different LED Light Intensities and 6-Benzyladenine Concentrations in Relation to Shoot Development, Leaf Architecture, and Photosynthetic Pigments of Gerbera jamesonii Bolus In Vitro

Agronomy ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 358 ◽  
Author(s):  
Monika Cioć ◽  
Andrzej Kalisz ◽  
Marek Żupnik ◽  
Bożena Pawłowska
Keyword(s):  
Photosynthetic Pigments ◽  
Photosynthetic Pigment ◽  
Shoot Multiplication ◽  
Dry Weight ◽  
Photon Flux ◽  
Gerbera Jamesonii ◽  
Leaf Architecture ◽  
Weight Content ◽  
Blade Area

A mixture of red and blue light-emitting diodes (LEDs; at a ratio of 7:3, respectively) were used to analyze the effects of different photosynthetic photon flux densities (PPFDs) (40, 80, and 120 µmol m−2 s−1 hereafter known as LED 40, 80, and 120, respectively) on the micropropagation of Gerbera jamesonii Bolus shoots. The experiment also examined the effect of 6-benzyladenine (BA) in 1, 2.5, and 5 µM concentrations in the media. Biometrical observations and analyses of leaf morphometry and photosynthetic pigment content were conducted. Shoot multiplication increased with an increasing BA concentration. A PPFD of 80 µmol m−2 s−1 and 5 µM BA is suggested as efficient for shoot propagation and economically viable. LED 120 increased the leaf blade area and its width, and circularity and elongation ratios. The intensity of light did not affect the fresh weight, which increased at higher BA concentrations (2.5 and 5 μM). The dry weight content decreased with increasing cytokinin concentration; the greatest content was observed on media with 1 µM BA under PPFD 120 µmol m−2 s−1. LED 80 increased the photosynthetic pigments content in the leaves in comparison to the standard intensity of LED 40. Increased BA concentration raises the content of chlorophyll a.

scholarly journals Effects of Triazole and Strobilurin-Based Fungicides on Fusarium culmorum on Wheat

2019 ◽  
Vol 11 (5) ◽  
pp. 16
Author(s):  
Füsun Sukut ◽  
Nagehan Desen Köycü
Keyword(s):  
Disease Severity ◽  
Germination Rate ◽  
Fusarium Culmorum ◽  
Dry Weight ◽  
Fusarium Spp ◽  
Coleoptile Length ◽  
Crown Root ◽  
Cultural Methods

Fusarium culmorum is an important pathogen, that causes pre and post-emergence death in seedlings from both seed and soil-borne sources, in addition to causing root, crown root and wheat head infections on wheat. Treatment of seeds with fungicides is especially important to inhibit seed originated seedling infections when cultural methods are ineffective against the infection of the pathogen in root and crown root and the lack of completely resistant wheat cultivars against this pathogen. The efficiency of fungicides with prothioconazole + tebuconazole and triticonazole + pyraclostrobin active ingredients, licensed on Fusarium spp. in seeds, on seed originated seedling infections of F. culmorum is determined in vitro and in vivo conditions. Seeds of Flamura-85 bread wheat cultivar, naturally infected with F. culmorum S-14, were used in this study. The effect of treating seeds naturally infected with F. culmorum S-14 with fungicides on seed germination, root length, coleoptile length and disease severity was determined in the experiment in vitro. Germination rate of the seed was determined as 68% for triticonazole + pyraclostrobin and 43% for prothioconazole + tebuconazole, while disease severity was 42.70% and 61.30%, respectively. While both fungicides were determined to be effective on the disease severity, the effect of triticonazole + pyraclostrobin on disease severity was found to be higher than prothioconazole + tebuconazole. Because of the higher rate of effectiveness of triticonazole + pyraclostrobin to prevent seed-borne infections in vitro conditions, it was determined that the fungicide causes an increase in germination rate with wet and dry weight of the seed, while significantly decreasing the disease severity, in the tests to determine the effectiveness of the fungicide in vivo conditions.

scholarly journals Biochemical Response and Nutrient Uptake of Two Arbuscular Mycorrhiza-Inoculated Chamomile Varieties under Different Water Potentials

2021 ◽  
Author(s):  
Fatemeh Ebrahimi ◽  
Amin Salehi ◽  
Mohsen Movahedi Dehnavi ◽  
Amin Mirshekari ◽  
Mohammad Hamidian ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Arbuscular Mycorrhiza ◽  
Water Deficit ◽  
Crop Production ◽  
Soluble Sugars ◽  
Am Fungi ◽  
Dry Weight ◽  
Water Deficit Stress ◽  
Randomized Design ◽  
Total Dry Weight

Abstract BackgroundWater-deficit stress is one of the most important sources of damage to crop production worldwide. Adopting appropriate varieties using soil microorganisms such as arbuscular mycorrhiza(AM) fungi can significantly reduce the adverse effects of water deficiency. This study is aimed to evaluate the role of Funneliformis mosseae on nutrients uptake and some physiological traits of two chamomile varieties namely Bodgold (Bod) and Soroksári (Sor) under water-deficit stress. The pot experiment was performed in a completely randomized design with three factors: water-deficit stress (PEG 6000) was applied along with Hoagland solution at three levels (0, -0.4 and -0.8 MPa), two German chamomile varieties (Bodgold (Bod) and Soroksari (Sor)) and AM inoculation (Funneliformis mosseae species (fungal and non-fungal)) at four replications in perlite substrate. ResultsWater-deficit stress significantly reduced the uptake of macro-nutrients (N, P, and K) and micro-nutrients (Fe, Cu, Mn, and Zn) in the shoots and roots. Moreover, the level of osmolytes (total soluble sugars and proline) and the activity of antioxidant enzymes in the shoots of both varieties increased under water-deficit stress. In the case of Sor variety, the level of these compounds was more satisfactory. AM improved plant nutrition uptake and osmolyte contents while enhancing antioxidant enzymes and reducing the adverse effects of water-deficit stress. Under water-deficit stress, the growth and total dry weight improved upon AM inoculation. ConclusionsIn general, inoculation of chamomile with AM balanced the uptake of nutrients increased the level of osmolytes, antioxidant enzymes, and hence improved plant characteristics under water-deficit stress in both varieties, however, it was more effective in reducing stress damages in Sor variety.

scholarly journals Effect of Polyethylene Glycol Induced Water Stress on Germination and Seedling Growth of Wheat (Triticum aestivum)

2017 ◽  
Vol 15 (1) ◽  
pp. 81-91 ◽  
Author(s):  
MS Rana ◽  
MA Hasan ◽  
MM Bahadur ◽  
MR Islam
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Stress Tolerance ◽  
Water Deficit ◽  
Seedling Growth ◽  
Dry Weight ◽  
Stress Sensitivity ◽  
Water Deficit Stress ◽  
Growth Stages ◽  
Wheat Genotypes

The performance of twenty wheat genotypes under Polyethylene Glycol (PEG) induced water stress during germination and early seedling growth stages were tested under three levels of water potential i) Control (Tap water), ii) -2 bars and iii) -4 bar at the Crop Physiology and Ecology Laboratory of Hajee Mohammad Danesh Science and Technology University, Dinajpur during September 2014 to October 2014. Rate of germination and vigor index of all wheat genotypes were delayed with the increment of water stress induced by PEG. Shoot and root lengths and seedling dry weight of 10 days old seedlings were found to be reduced due to the increment of water stress. However, the degree of reduction of these parameters with the increment of water stress was not similar for all wheat genotypes. Stress tolerance index (STI) based on seedling dry weight indicated a wide difference in stress tolerance among the wheat genotypes. At moderate water deficit stress, BARI Gom 25, E 34, E 28 and BAW 1170 showed more stress tolerance and the wheat genotypes- Sourav, E 23 and BAW 1140 showed greater stress sensitivity than the other wheat genotypes. At higher water deficit stress, BARI Gom 25, BARI Gom 28, E 28 and BAW 1170 showed more stress tolerance and the wheat genotypes- Satabdi, Sourav, BARI Gom 26, E 23, E 38, E 24, BAW 1163, BAW 1140 and BAW 1151 showed greater stress sensitivity than the others. Considering both moderate and high water deficit stress, BARI Gom 25, E 28 and BAW 1170 were found as tolerant and Sourav, E 23 and BAW 1140 were found as water deficit stress sensitive wheat genotypes. The Agriculturists 2017; 15(1) 81-91

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