Morphological Markers of Pathological Changes in Bitches Endometrium Affected by a Cystic Endometrial Hyperplasia - Pyometra Complex (CEH-P)

Abstract Background The aim of the analysis was to designate the morphological symptoms that appear during the Cystic Endometrial Hyperplasia - Pyometra complex (CEH-P), diagnosed by microscopic examination. The investigation were conducted on the uteri of 120 bitches in age between 1–16. The microscopic examinations were based on histological stainings. The aim of examinations was to find the differences in morphology of endometrium in the specimens of the uteri wall (H&E stain). All of the uteri were divided into three pathological groups (GI – GIII), created on the basis of clinically symptoms of analyzed diseases. Uteri without symptoms were classified as a control group (C).Results Histopathological analysis that were conducted have confirmed preliminary macroscopic evaluation for control group with unchanged uteri, group GII with cystic endometrial hyperplasia of uteri (CEH), and group GIII with uteri with pyometra. The confirmation of compatibility of both macroscopic and microscopic evaluation of the uteri were observed in groups GII and GIII. In the uteri of the group GI a severe congestion of endometrium have been observed – it is typical for the inflammation – which was not confirmed during histopathological examinations. Those examinations revealed only endometrial haemorrhage.Conclusions The results are showing that during classification of research material microscopic examinations are required. The diagnose based on the macroscopic changes in typical for CEH-P symptoms might be incorrect, if it is not supported by detailed research. More than that, in all uteri with the closed pyometra the CEH was also observed. It suggest that pyometra may occur as a consequence of cystic endometrial hyperplasia and bacterial infections ended with inflammation. The results obtained can be used to create a basis for pathologic classification of endometrial hyperplasia, including CEH-P complex in bitches.

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The morphological changes in bitches endometrium affected by Cystic Endometrail Hyperplasia - Pyometra complex – the value of routine histopathological examination

10.21203/rs.3.rs-26656/v2 ◽

2020 ◽

Author(s):

Magdalena Woźna-Wysocka ◽

Marta Rybska ◽

Beata Błaszak ◽

Bartłomiej Maria Jaśkowski ◽

Magdalena Kulus ◽

...

Keyword(s):

Bacterial Infections ◽

Endometrial Hyperplasia ◽

Histopathological Examination ◽

Morphological Changes ◽

Control Group ◽

Histopathological Analysis ◽

Research Material ◽

Microscopic Evaluation ◽

Pathologic Classification

Abstract Background: The aim of the analysis was to designate the morphological symptoms that appear during the Cystic Endometrial Hyperplasia - Pyometra complex (CEH-P), diagnosed by microscopic examination. The investigation were conducted on the uteri of 120 bitches in age between 1 – 16. The microscopic examinations were based on histological stainings. The aim of examinations was to find the differences in morphology of endometrium in the specimens of the uteri wall (H&E stain). All of the uteri were divided into three pathological groups (GI – GIII), created on the basis of macroscopic examinations of analyzed diseases. Uteri without macroscopic pathological changes were classified as a control group (C).Results: Histopathological analysis that were conducted have confirmed preliminary macroscopic evaluation for control group with unchanged uteri, group GII with cystic endometrial hyperplasia of uteri (CEH), and group GIII with uteri with pyometra. The confirmation of compatibility of both macroscopic and microscopic evaluation of the uteri were observed in groups GII and GIII. In the uteri of the group GI a severe congestion of endometrium have been observed – it is typical for the inflammation – which was not confirmed during histopathological examinations. Those examinations revealed only endometrial haemorrhage.Conclusions: The results are showing that during classification of research material microscopic examinations are required. The diagnose based on the macroscopic changes in typical for CEH-P symptoms might be incorrect, if it is not supported by detailed research. More than that, in all uteri with the closed pyometra the CEH was also observed. It suggest that pyometra may occur as a consequence of cystic endometrial hyperplasia and bacterial infections ended with inflammation. The results obtained can be used to create a basis for pathologic classification of endometrial hyperplasia and indicate typical morphological changes in endometrium during development of CEH-P complex in bitches.

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Morphological changes in bitches endometrium affected by cystic endometrial hyperplasia - pyometra complex – the value of histopathological examination

BMC Veterinary Research ◽

10.1186/s12917-021-02875-0 ◽

2021 ◽

Vol 17 (1) ◽

Author(s):

Magdalena Woźna-Wysocka ◽

Marta Rybska ◽

Beata Błaszak ◽

Bartłomiej M. Jaśkowski ◽

Magdalena Kulus ◽

...

Keyword(s):

Endometrial Hyperplasia ◽

Histological Analysis ◽

Histopathological Examination ◽

Morphological Changes ◽

Clinical Signs ◽

Control Group ◽

Pathological Changes ◽

Microscopic Evaluation ◽

Uterine Diseases

Abstract Background Cystic endometrial hyperplasia-pyometra complex (CEH-P) is one of the most common uteropathies in bitches. In diseases with mild or obscure clinical signs and normal uterine size, a diagnosis based on a clinical assessment might be incorrect. The main aim of the research was to determine the morphological variables accompanying uterine diseases in bitches in microscopic evaluation. Consequently, the obtained results can be used to create a new classification system for uterine pathological changes during the development of the CEH-P, diagnosed by microscopic examination in bitches. Material for the study consisted of the uteri of 120 female dogs, aged 1–16 years, obtained during routine ovariohysterectomies. Macroscopic observation after a longitudinal incision of the uterine horns, allowed a preliminary classification of the uteri into research groups: control group (physiological uteri), and groups GI-III uteri collected form bitches with varying degrees of endometrial pathology. These preliminary classifications were then verified by histological analysis (H&E stain). Results The obtained results made it possible to determine and describe the prevalence (%) of pathological changes characteristic of the analyzed uterine diseases in the examined bitches. Histopathological analyses that were conducted have confirmed preliminary macroscopic evaluation for the control group, group GII (CEH), and group GIII (pyometra). In the uteri of the GI group, a severe congestion of the endometrium has been observed – this is typical of inflammation – which was not confirmed during histopathological examinations. However, these examinations revealed acute endometrial haemorrhage of varying severity. Conclusions Early reproduction disorders in bitches are, in general, not confirmed by clinical signs in the examined animals. The results show that during classification of typical morphological changes in the endometrium over the development of the CEH-P complex in bitches microscopic examinations are required. The obtained results indicate a frequent lack of consistency in the macroscopic assessment and histological analysis of the endometrium, observed in the analyzed uterine diseases, which in most cases is not followed by clinical symptoms. The presented classification of uterine diseases may be useful as a diagnostic tool in reproductive disorders in bitches and in examination in the field of basic research.

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The morphological changes in bitches endometrium affected by Cystic Endometrail Hyperplasia - Pyometra complex – the value of histopathological examination

10.21203/rs.3.rs-26656/v3 ◽

2020 ◽

Author(s):

Magdalena Woźna-Wysocka ◽

Marta Rybska ◽

Beata Błaszak ◽

Bartłomiej Maria Jaśkowski ◽

Magdalena Kulus ◽

...

Keyword(s):

Histological Analysis ◽

Clinical Symptoms ◽

Histopathological Examination ◽

Morphological Changes ◽

Clinical Signs ◽

Control Group ◽

Pathological Changes ◽

Microscopic Evaluation ◽

Uterine Diseases

Abstract Background: The cystic endometrial hyperplasia-pyometra complex (CEH-P) is one of the most common uteropathies in bitches. In diseases with mild or obscure clinical signs and normal uterine size, a diagnosis based on a clinical assessment might be incorrect. The main aim of the research was to determine the morphological variables accompanying uterine diseases in bitches in microscopic evaluation. Consequently, the obtained results can be used to create a new classification system for uterine pathological changes during the development of the CEH-P, diagnosed by microscopic examination in bitches. Material for the study consisted of the uteri of 120 female dogs, aged 1-16 years, obtained during routine ovariohysterectomies. Macroscopic observation after a longitudinal incision of the uterine horns, allowed a preliminary classification of the uteri into research groups: control group (physiological uteri), and groups GI-III uteri collected form bitches with varying degrees of endometrial pathology. These preliminary classifications were then verified by histological analysis (H&E stain).Results: The obtained results made it possible to determine and describe the prevalence (%) of pathological changes characteristic of the analyzed uterine diseases in the examined bitches. Histopathological analyses that were conducted have confirmed preliminary macroscopic evaluation for the control group, group GII (CEH), and group GIII (pyometra). In the uteri of the GI group, a severe congestion of endometrium has been observed – this is typical of inflammation – which was not confirmed during histopathological examinations. However, these examinations revealed acute endometrial haemorrhage of varying severity.Conclusions: Early reproduction disorders in bitches are, in general, not confirmed by clinical signs in the examined animals. The results show that during classification of typical morphological changes in endometrium over the development of the CEH-P complex in bitches microscopic examinations are required. The obtained results indicate a frequent lack of consistency in the macroscopic assessment and histological analysis of the endometrium, observed in the analyzed uterine diseases, which in most cases is not followed by clinical symptoms. The presented classification of uterine diseases may be useful as a diagnostic tool in reproductive disorders in bitches and in examination in the field of basic research.

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The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice

Toxins ◽

10.3390/toxins10110449 ◽

2018 ◽

Vol 10 (11) ◽

pp. 449 ◽

Cited By ~ 7

Author(s):

Nan Wang ◽

Peng Li ◽

Mingyang Wang ◽

Si Chen ◽

Sheng Huang ◽

...

Keyword(s):

Water System ◽

Protective Role ◽

Feed Additive ◽

Control Group ◽

Histopathological Analysis ◽

Distilled Water ◽

Pathological Changes ◽

Bacillus Velezensis ◽

Toxic Damage ◽

Fermented Feed

Zearalenone (ZEN) is an estrogen-like mycotoxin produced by Fusarium that seriously compromises the safety of animal and human health. In this study, our aim was to evaluate the protective effect of Bacillus velezensis A2 against biochemical and pathological changes induced by zearalenone in mice. Kunming mice (n = 40; 25 ± 2 g) were allotted to four treatment groups: a control group (basic feed); a ZEN group (basic feed with a ZEN dose of 60 mg/kg); an A2 strain fermented feed group (150 g of feed mixed with 150 mL of sterile distilled water and inoculated with 5 mL of phosphate buffer salt (PBS) resuspended A2 strain); and an A2 strain fermented ZEN-contaminated feed group. (A2 strain group 150 mL pure bacterial distilled water system mixed with 150 g ZEN-contaminated feed.) Our results showed that the Bacillus velezensis A2 strain can completely degrade the ZEN-contaminated feed within 5 days. (The concentration of ZEN in fermentation was 60 μg/mL.) After the mice fed for 28 days, compared with the control group, the activities of AST and ALT were increased, the activities of glutathione peroxidase (GSH-PX) and total superoxide dismutase (T-SOD) were decreased, and the amount of creatinine (CRE), blood urea nitrogen (BUN), uric acid (UA), and malondialdehyde (MDA) in the ZEN group were increased in the mice serum (p < 0.05; p < 0.01). However, compared with the ZEN group, these biochemical levels were reversed in the A2 strain fermented feed group and in the A2 strain fermented ZEN-contaminated feed group (p < 0.05; p < 0.01). Furthermore, histopathological analysis only showed pathological changes of the mice liver in the ZEN group. The results showed that Bacillus velezensis A2 as additive could effectively remove ZEN contamination in the feed and protect the mice against the toxic damage of ZEN. In conclusion, Bacillus velezensis A2 has great potential use as a microbial feed additive to detoxify the toxicity of zearalenone in production practice.

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Cerebrospinal fluid neopterin as a biomarker of neuroinflammatory diseases

Scientific Reports ◽

10.1038/s41598-020-75500-z ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Marta Molero-Luis ◽

Didac Casas-Alba ◽

Gabriela Orellana ◽

Aida Ormazabal ◽

Cristina Sierra ◽

...

Keyword(s):

Cerebrospinal Fluid ◽

Discriminant Analysis ◽

Bacterial Infections ◽

Pediatric Patients ◽

Canonical Discriminant Analysis ◽

Control Group ◽

Csf Biomarkers ◽

Molecular Testing ◽

Respiratory Pathogens

Abstract The elevation of neopterin in cerebrospinal fluid (CSF) has been reported in several neuroinflammatory disorders. However, it is not expected that neopterin alone can discriminate among different neuroinflammatory etiologies. We conducted an observational retrospective and case–control study to analyze the CSF biomarkers neopterin, total proteins, and leukocytes in a large cohort of pediatric patients with neuroinflammatory disorders. CSF samples from 277 subjects were included and classified into four groups: Viral meningoencephalitis, bacterial meningitis, acquired immune-mediated disorders, and patients with no-immune diseases (control group). CSF neopterin was analyzed with high-performance liquid chromatography. Microbiological diagnosis included bacterial CSF cultures and several specific real-time polymerase chain reactions. Molecular testing for multiple respiratory pathogens was also included. Antibodies against neuronal and glial proteins were tested. Canonical discriminant analysis of the three biomarkers was conducted to establish the best discriminant functions for the classification of the different clinical groups. Model validation was done by biomarker analyses in a new cohort of 95 pediatric patients. CSF neopterin displayed the highest values in the viral and bacterial infection groups. By applying canonical discriminant analysis, it was possible to classify the patients into the different groups. Validation analyses displayed good results for neuropediatric patients with no-immune diseases and for viral meningitis patients, followed by the other groups. This study provides initial evidence of a more efficient approach to promote the timely classification of patients with viral and bacterial infections and acquired autoimmune disorders. Through canonical equations, we have validated a new tool that aids in the early and differential diagnosis of these neuroinflammatory conditions.

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Clinical and sonological features in endometriomas in women of reproductive age

HEALTH OF WOMAN ◽

10.15574/hw.2016.114.85 ◽

2016 ◽

pp. 85-89

Author(s):

О. Shapoval ◽

Keyword(s):

Endometrial Hyperplasia ◽

Benign Tumor ◽

Pain Syndrome ◽

Reproductive Age ◽

Control Group ◽

Women Of Reproductive Age ◽

Ovarian Endometriosis ◽

Gynecological Examination ◽

Different Characteristics ◽

Ovarian Endometriomas

The objective: to study the prevalence of ovarian endometriosis in women of reproductive age, the features of clinical and ultrasound picture of endometriosis. Patients and methods. The study involved 22 patients with endometriomas, the control group – 50 women gynecological and somatically healthy. Results. The incidence of ovarian endometriomas in the structure of benign tumor-like formations of ovaries is 0.62%. In 72.73% of ovarian endometriomas occur on a background of concomitant gynecological pathology. Clinically, in 77.27% of cases there is a pain syndrome, in 59.09% – algomenorrhea, in 13.64% – infertility; 18.18% of cases endometriomas remain «dumb» and proceed with the erased clinical picture. Sonologically in patients with endometriomas adenomyosis, endometrial hyperplasia, changes in the contralateral ovary are determined. Conclusion. Without additional methods of diagnostic gynecological examination may identify the 3 cm tumor-like formation of the ovary with different characteristics, which does not allow to differentiate endometrioma from inflammation, functional and ovarian tumors. Ultrasound can diagnose pathological ovarian formation of 1 cm, detailing the nature of the cyst. Key words: endometriosis, reproductive age, retrospective analysis, ultrasound.

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STUDY OF ORNAMENTS IN HUNTO SULTAN AMAY MOSQUE GORONTALO

ARTic ◽

10.34010/artic.v4i0.2418 ◽

2019 ◽

Vol 4 ◽

pp. 167-176

Author(s):

Risti Puspita Sari Hunowu

Keyword(s):

Qualitative Method ◽

The Other ◽

Visual Form ◽

Research Material ◽

Other Hand ◽

The World ◽

Existing Form ◽

The City ◽

Dutch Colonial

This research is aimed at studying the Hunto Sultan Amay Mosque located in Gorontalo City. Hunto Sultan Amay Mosque is the oldest mosque in the city of Gorontalo The Hunto Sultan Amay Mosque was built as proof of Sultan Amay's love for a daughter and is a representation of Islam in Gorontalo. Researchers will investigate the visual form of the Hunto Sultan Amay Mosque which was originally like an ancient mosque in the archipelago. can be seen from the shape of the roof which initially used an overlapping roof and then converted into a dome as well as mosques in the world, we can be sure the Hunto Sultan Amay Mosque uses a dome roof after the arrival of Dutch Colonial. The researcher used a qualitative method by observing the existing form in detail from the building of the mosque with an aesthetic approach, reviewing objects and selecting the selected ornament giving a classification of the shapes, so that the section became a reference for the author as research material. Based on the analysis of this thesis, the form of the Hunto Sultan Amay mosque as well as the mosques located in the archipelago and the existence of ornaments in the Hunto Sultan Amay Mosque as a decorative structure support the grandeur of a mosque. On the other hand, Hunto Mosque ornaments reveal a teaching. The form of a teaching is manifested in the form of motives and does not depict living beings in a realist or naturalist manner. the decorative forms of the Hunto Sultan Sultan Mosque in general tend to lead to a form of flora, geometric ornaments, and ornament of calligraphy dominated by the distinctive colors of Islam, namely gold, white, red, yellow and green.

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Dendrobium officinalis Flower Improves Learning and Reduces Memory Impairment by Mediating Antioxidant Effect and Balancing the Release of Neurotransmitters in Senescent Rats

Combinatorial Chemistry & High Throughput Screening ◽

10.2174/1386207323666200407080352 ◽

2020 ◽

Vol 23 (5) ◽

pp. 402-410 ◽

Cited By ~ 1

Author(s):

Lin-Zi Li ◽

Shan-Shan Lei ◽

Bo Li ◽

Fu-Chen Zhou ◽

Ye-Hui Chen ◽

...

Keyword(s):

Learning And Memory ◽

Brain Aging ◽

Morris Water Maze Test ◽

Control Group ◽

Histopathological Analysis ◽

Hippocampal Damage ◽

Common Belief ◽

Mao B ◽

Positive Effects ◽

The Brain

Aim and Objective: The Dendrobium officinalis flower (DOF) is popular in China due to common belief in its anti-aging properties and positive effects on “nourish yin”. However, there have been relatively few confirmatory pharmacological experiments conducted to date. The aim of this work was to evaluate whether DOF has beneficial effects on learning and memory in senescent rats, and, if so, to determine its potential mechanism of effect. Materials and Methods: SD rats were administrated orally DOF at a dose of 1.38, or 0.46 g/kg once a day for 8 weeks. Two other groups included a healthy untreated control group and a senescent control group. During the 7th week, a Morris water maze test was performed to assess learning and memory. At the end of the experiment, serum and brain samples were collected to measure concentrations of antioxidant enzymes, including malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GSH-Px) in serum, and the neurotransmitters, including γ-aminobutyric acid (γ-GABA), Glutamic (Glu), and monoamine oxidase B (MAO-B) in the brain. Histopathology of the hippocampus was assessed using hematoxylin-eosin (H&E) staining. Results: The results suggested that treatment with DOF improved learning as measured by escape latency, total distance, and target quadrant time, and also increased levels of γ-GABA in the brain. In addition, DOF decreased the levels of MDA, Glu, and MAO-B, and improved SOD and GSHPx. Histopathological analysis showed that DOF also significantly reduced structural lesions and neurodegeneration in the hippocampus relative to untreated senescent rats. Conclusion: DOF alleviated brain aging and improved the spatial learning abilities in senescent rats, potentially by attenuating oxidative stress and thus reducing hippocampal damage and balancing the release of neurotransmitters.

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Rapid uropathogen identification using surface enhanced Raman spectroscopy active filters

Scientific Reports ◽

10.1038/s41598-021-88026-9 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Simon D. Dryden ◽

Salzitsa Anastasova ◽

Giovanni Satta ◽

Alex J. Thompson ◽

Daniel R. Leff ◽

...

Keyword(s):

Raman Spectroscopy ◽

Bacterial Infections ◽

Surface Enhanced Raman Spectroscopy ◽

Active Filters ◽

Raman Signal ◽

Rapid Diagnostics ◽

Bacterial Classification ◽

Surface Enhanced ◽

Surface Enhanced Raman

AbstractUrinary tract infection is one of the most common bacterial infections leading to increased morbidity, mortality and societal costs. Current diagnostics exacerbate this problem due to an inability to provide timely pathogen identification. Surface enhanced Raman spectroscopy (SERS) has the potential to overcome these issues by providing immediate bacterial classification. To date, achieving accurate classification has required technically complicated processes to capture pathogens, which has precluded the integration of SERS into rapid diagnostics. This work demonstrates that gold-coated membrane filters capture and aggregate bacteria, separating them from urine, while also providing Raman signal enhancement. An optimal gold coating thickness of 50 nm was demonstrated, and the diagnostic performance of the SERS-active filters was assessed using phantom urine infection samples at clinically relevant concentrations (105 CFU/ml). Infected and uninfected (control) samples were identified with an accuracy of 91.1%. Amongst infected samples only, classification of three bacteria (Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae) was achieved at a rate of 91.6%.

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Effect of a single or two doses of an anti-GnRH vaccine on testicle morpho-functional characteristics in Nelore bulls

Tropical Animal Health and Production ◽

10.1007/s11250-021-02600-x ◽

2021 ◽

Vol 53 (1) ◽

Author(s):

Emanuel M. Doroteu ◽

Joao H. M. Viana ◽

Jair A. Ferreira Junior ◽

Juliana T. A. Macedo ◽

Rodrigo A. Oliveira ◽

...

Keyword(s):

Bos Taurus ◽

Active Immunization ◽

Control Group ◽

Histopathological Analysis ◽

Sample Collection ◽

Functional Characteristics ◽

Scrotal Circumference ◽

Bos Taurus Indicus ◽

Boost Dose ◽

And Control

AbstractThe aim of this study was to compare testicle morpho-functional characteristics in bulls undergoing a single or two immunizations against GnRH. Nelore (Bos taurus indicus) bulls were randomly allocated into three experimental groups: G1 (n=12), a single 400 μg dose of anti-GnRH vaccine on day 0; G2 (n=11), a first 400 μg dose of anti-GnRH vaccine on day 0 followed by a second (boost) dose 30 days later; and control group (CG, n=12), 1 mL saline 0.9% at day 0. Every 30 days, from day 0 until slaughter at day 90, the bulls were weighed and underwent testicular biometry, semen collection and analysis, and blood sample collection for testosterone measurement. Immediately after slaughter, the testicles were removed and transport at 15°C to the laboratory for histopathological analysis. There was a decrease in testicular height (P=0.0476), width (P=0.0021), and in scrotal circumference (P=0.0001), after either a single (G1) or two (G2) immunizations against GnRH. Both G1 and G2 had lower testosterone concentrations than CG from day 60 on (P<0.01), but in G2, it was also lower than in G1 at day 90 (P=0.0006). All sperm parameters were affected by active immunization against GnRH (P<0.05), and in G2, averages were lesser (P<0.05) than in G1 from day 60 on. No signs of seminiferous tubule degeneration were found in any sample from the CG, contrasting with 75.0% and 100.0% of the samples from G1 and G2, respectively. In summary, immunocastration affected testicle morpho-functional characteristics in bulls in a time- and dose-dependent way.

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