Serum levels of Vitamin D3, Zinc, and Parathyroid Hormone in HCV-induced Hepatocellular Carcinoma.

Abstract Background and AimThe role of the metabolic syndrome in hepatocellular carcinoma (HCC) has been previously reported. This study aims to investigate the possible role of vitamin D3, Zinc, Parathyroid hormone (PTH), calcium and phosphorus serum levels as non-traditional metabolic risk factors in HCV-related HCC.MethodThis cross-sectional observational study recruited HCV infected patients with and without HCC. All patients were subjected to demographic, biochemical, and hematological assessment. Serum levels of vitamin D3, Zinc, PTH, calcium, and phosphorus were determined in all the study participants.ResultsThis study includes 50 patients with HCV-related HCC compared to 40 patients with HCV-related liver cirrhosis and 30 patients with HCV chronic hepatitis C (CHC) without HCC. Our results show significantly higher age, male sex, aspartate transaminase (AST), PTH and corrected serum calcium levels in the HCC patients compared to values in the other two groups, (p < 0.001); while significant lower vitamin D3 and zinc levels were detected among the HCC patients compared to patients with non-HCC liver cirrhosis and CHC, (p < 0.001).Vitamin D3 deficiency was detected in 96% of the HCC patients, while it was detected in only 22.5% of the cirrhotic patients and in none of the CHC patients, (p < 0.001). However, on multiple stepwise regression analysis, only the age, AST, PTH, and corrected calcium levels were the independent predictors for HCC when studied in relation to chronic liver disease.ConclusionThis study indicates the prevalent deficient levels of vitamin D3 and zinc in HCC patients; however, a causal relationship is not established in this study.

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The role of chemokine CC ligand 20 in patients with liver cirrhosis and hepatocellular carcinoma

The International Journal of Biological Markers ◽

10.5301/jbm.2012.9097 ◽

2012 ◽

Vol 27 (2) ◽

pp. 125-131 ◽

Cited By ~ 8

Author(s):

Hanan H. Soliman ◽

Hala Nagy ◽

Nesreen Kotb ◽

Mohamed A. Alm El-Din

Keyword(s):

Hepatocellular Carcinoma ◽

Liver Cirrhosis ◽

Predictive Value ◽

Serum Levels ◽

Study Groups ◽

Cross Sectional Study ◽

Cross Sectional ◽

Asymptomatic Patients ◽

Group I

Background and aim To evaluate the role of chemokine CC ligand 20 (CCL20) as a biomarker for hepatocellular carcinoma (HCC). Patients and methods Ninety patients in four groups were enrolled in this prospective cross-sectional study: 30 with HCC (group I), 30 with liver cirrhosis (group II), 15 with hepatitis C virus infection (group III), and 15 healthy blood donors as controls. Alpha fetoprotein (AFP), CCL20 and vascular endothelial growth factor (VEGF) were measured in all groups. Results Serum levels of CCL20 were significantly different among the study groups (F=230.979, p<0.001). The highest level was found in HCC patients (57.305 ± 6.386 pg/mL) followed by patients with cirrhosis (45.999 ± 5.165 pg/mL) compared with 22.781 ± 5.986 pg/mL and 18.585 ± 3.554 pg/mL in asymptomatic patients with HCV infection and controls, respectively. In HCC patients, CCL20 significantly correlated with VEGF (r=0.559, p=0.001), AFP (r=0.814, p<0.001), Child score (r=0.748, p<0.001), and tumor size (r=0.825, p<0.001). The cutoff value of CCL20 for the detection of HCC in HCV-infected patients was 54 pg/mL with 93.1% accuracy, 89.6% negative predictive value, 92.6% positive predictive value, 83.3% sensitivity, and 93.3% specificity. In patients with cirrhosis, CCL20 significantly correlated with VEGF (r=0.455, p=0.011), AFP (r=0.975, p<0.001), and Child score (r=0.977, p<0.001). Conclusion CCL20 may be used for the detection of HCC in HCV-infected patients with comparable specificity and higher sensitivity than AFP.

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SUBLINGUAL VITAMIN D3 DRUG THERAPY IN VITAMIN D DEFICIENCY PATIENTS AT PRAVARA RURAL HOSPITAL

International Journal of Clinical and Biomedical Research ◽

10.31878/ijcbr.2019.54.05 ◽

2019 ◽

pp. 18-20

Author(s):

Sanjeeva Kumar Goud T ◽

Rahul Kunkulol

Keyword(s):

Vitamin D ◽

Vitamin D Deficiency ◽

Vitamin D3 ◽

Serum Vitamin ◽

Cross Sectional ◽

Serum Vitamin D ◽

Significant Difference ◽

Before And After

The present study was aimed to study the effect of Sublingual Vitamin D3 on Serum Vitamin D level in Vitamin D deficiency patients. This was a cross-sectional and interventional study. All the Vitamin D deficiency patients of age 18-60years and either gender, willing to participate in the study were included. Patients who had greater than 20 ng/ml were excluded from the study. The total number of participants in our study was 200, out of these 111 males and 89 females, the mean age in our study was 51.07 ± 7.39Yrs. All volunteers were given sublingual vitamin D3 (60,000IU) in six doses every fifteen days of follow up for 3 months. The subject’s serum 25(OH)D levels were estimated before and after the treatment of sublingual vitamin D3. There was a statistically significant difference in serum vitamin D3 level before 16.61±6.71 ng/ml and after 35.80±7.80 ng/ml after treatment with Sublingual Vitamin D3. Six doses of 60,000IU of Vitamin D3 sublingual route having improved the role of serum 25(OH)D levels in the treatment of Vitamin D3 deficiency patients.Keywords: Vitamin D3; Sublingual route

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Multiplexed Proteomic Approach for Identification of Serum Biomarkers in Hepatocellular Carcinoma Patients with Normal AFP

Journal of Clinical Medicine ◽

10.3390/jcm9020323 ◽

2020 ◽

Vol 9 (2) ◽

pp. 323

Author(s):

Young-Sun Lee ◽

Eunjung Ko ◽

Eileen L. Yoon ◽

Young Kul Jung ◽

Ji Hoon Kim ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Liver Cirrhosis ◽

Cell Lines ◽

Cellular Proliferation ◽

Hepatoma Cell ◽

Multiple Reaction Monitoring ◽

Serum Levels ◽

Human Hepatoma Cell ◽

Hepatoma Cell Lines ◽

Proteomic Approach

Alpha fetoprotein (AFP) has been used as a serologic indicator of hepatocellular carcinoma (HCC). We aimed to identify an HCC-specific serum biomarker for diagnosis using a multiplexed proteomic technique in HCC patients with normal AFP levels. A total of 152 patients were included from Guro Hospital, Korea University. Among 267 identified proteins, 28 and 86 proteins showed at least a two-fold elevation or reduction in expression, respectively. Multiple reaction monitoring (MRM) analysis of 41 proteins revealed 10 proteins were differentially expressed in patients with liver cirrhosis and HCC patients with normal AFP. A combination of tripartite motif22 (Trim22), seprase, and bone morphogenetic protein1 had an area under receiver operating characteristic of 0.957 for HCC diagnosis. Real-time PCR and western blot analysis of the paired tumor/non-tumor liver tissue in HCC revealed a reduced expression of Trim22 in the tumor tissue. Also, serum levels of Trim22 were significantly reduced in HCC patients with normal AFP compared to those with liver cirrhosis (p = 0.032). Inhibition of Trim22 increased cellular proliferation in human hepatoma cell lines, whereas overexpression of Trim22 decreased cellular proliferation in hepatoma cell lines. In conclusion, the combination of three serum markers improved the chance of diagnosing HCC. MRM-based quantification of the serum protein in patients with normal AFP provides the potential for early diagnosis of HCC.

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The Role of Dietary Calcium in the Physiology of Vitamin D Toxicity: Excess Dietary Vitamin-D3 Blunts Parathyroid Hormone Induction of Kidney 1-Hydroxylase

Archives of Biochemistry and Biophysics ◽

10.1006/abbi.1995.1328 ◽

1995 ◽

Vol 319 (2) ◽

pp. 535-539 ◽

Cited By ~ 32

Author(s):

M.J. Beckman ◽

J.A. Johnson ◽

J.P. Goff ◽

T.A. Reinhardt ◽

D.C. Beitz ◽

...

Keyword(s):

Vitamin D ◽

Parathyroid Hormone ◽

Vitamin D3 ◽

Dietary Calcium ◽

Dietary Vitamin ◽

Vitamin D Toxicity

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Genetic polymorphisms of interleukin-6 influence the development of hepatitis B virus-related liver cirrhosis

10.21203/rs.2.522/v1 ◽

2019 ◽

Author(s):

Caixia Xia ◽

Wei Zhu ◽

Chunhong Huang ◽

Guohua Lou ◽

Bingjue Ye ◽

...

Keyword(s):

Liver Cirrhosis ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Interleukin 6 ◽

Serum Levels ◽

Healthy Controls ◽

Nucleotide Polymorphisms ◽

Cross Sectional ◽

B Virus ◽

Infected Liver

Abstract Background Interleukin-6 (IL-6) plays an important role in chronic inflammation. Thus, we aimed to investigate the effects of IL-6 polymorphisms in predicting the progression of hepatitis B virus (HBV) -related liver cirrhosis. Methods A cross-sectional study was conducted to analysis IL-6 polymorphisms and serum levels of IL-6 in HBV-infected patients of different clinical phases and in healthy controls. IL-6 polymorphisms were detected by Taqman PCR method and plasma IL-6 levels were assessed by ELISA. Results Our analysis included 182 chronic hepatitis B (CHB) patients, 190 HBV-infected liver cirrhosis cases, 125 inactive HBsAg carriers, and 246 healthy controls. Seven SNPs in IL-6 including rs10499563, rs17147230, rs1800796, rs2069837, rs1524107, rs2066992, rs2069852 were analyzed. In haplotype analysis between HBV-infected liver cirrhosis cases with CHB patients, inactive HBV-carriers or healthy controls, haplotype CT in block 1 and haplotype GGCGG in block 2 were associated with liver cirrhosis (P<0.05). What’s more, the genotype or allele frequencies were significantly different in IL-6 rs10499563 and rs2069837 when HBV-infected liver cirrhosis patients compared with CHB patients, inactive HBV-carriers or healthy controls. A further study found that compared with the controls or CHB patients, plasma IL-6 was elevated in HBV-infected liver cirrhosis patients (P<0.05). Conclusion In conclusion, the polymorphisms of the IL-6 rs10499563 and rs2069837 are associated with the susceptibility of liver cirrhosis may through their effects on IL-6 expressions and these two single nucleotide polymorphisms can be used as potential predicting markers for prognosis of HBV-infected liver cirrhosis.

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Angiopoietin-2 Serum Levels Are Elevated in Patients With Liver Cirrhosis and Hepatocellular Carcinoma

The American Journal of Gastroenterology ◽

10.1111/j.1572-0241.2007.01377.x ◽

2007 ◽

Vol 102 (11) ◽

pp. 2471-2481 ◽

Cited By ~ 33

Author(s):

Arne Scholz ◽

Vanessa Annina Rehm ◽

Svenja Rieke ◽

Katja Derkow ◽

Petra Schulz ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Liver Cirrhosis ◽

Serum Levels ◽

Angiopoietin 2

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Diagnostic and prognostic role of SCCA-IgM serum levels in hepatocellular carcinoma (HCC)

Journal of Gastroenterology and Hepatology ◽

10.1111/jgh.12576 ◽

2014 ◽

Vol 29 (8) ◽

pp. 1637-1644 ◽

Cited By ~ 25

Author(s):

Caterina Pozzan ◽

Romilda Cardin ◽

Marika Piciocchi ◽

Nora Cazzagon ◽

Gemma Maddalo ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Serum Levels ◽

Prognostic Role

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Serum Zonulin in HBV-Associated Chronic Hepatitis, Liver Cirrhosis, and Hepatocellular Carcinoma

Disease Markers ◽

10.1155/2019/5945721 ◽

2019 ◽

Vol 2019 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

Xin Wang ◽

Ming-ming Li ◽

Ye Niu ◽

Xin Zhang ◽

Ji-bin Yin ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Liver Cirrhosis ◽

Chronic Hepatitis ◽

Diagnostic Accuracy ◽

Roc Curve ◽

Healthy Subjects ◽

Serum Levels ◽

Curve Analysis ◽

Advanced Stage ◽

Roc Curve Analysis

Background. The gut microbiota is involved in the occurrence and development of chronic liver diseases. Zonulin is considered a marker of intestinal permeability. The purpose of this study was to assess zonulin levels in patients with chronic hepatitis B (CHB), HBV-associated liver cirrhosis (LC), and HBV-associated hepatocellular carcinoma (HCC). Materials and Methods. The study population consisted of 90 HBV-associated HCC patients, 90 HBV-associated LC patients, 90 CHB patients, and 90 healthy subjects. Serum levels of zonulin and AFP were determined. The diagnostic accuracy of each marker was evaluated using receiver operating characteristic (ROC) curve analysis (AUC). Results. Serum zonulin levels were significantly higher in patients with HCC than in patients with LC or CHB or healthy subjects (p<0.001). Moreover, the zonulin levels were increased in the advanced stage of LC and HCC. ROC curve analysis revealed that serum zonulin could be used to differentiate CHB from cirrhosis. In addition, the combination of zonulin and AFP exhibited a significantly larger AUC compared with zonulin or AFP alone. Conclusions. Serum zonulin levels were significantly increased both in LC and in HCC and correlated with the advanced stage of LC and HCC. Moreover, the combination of zonulin and AFP confers significant benefit to diagnostic accuracy in differentiating LC from HCC.

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MicroRNA-140 inhibits skeletal muscle glycolysis and atrophy in endotoxin-induced sepsis in mice via the WNT signaling pathway

AJP Cell Physiology ◽

10.1152/ajpcell.00419.2018 ◽

2019 ◽

Vol 317 (2) ◽

pp. C189-C199 ◽

Cited By ~ 3

Author(s):

Li Liu ◽

Tian-Mei Li ◽

Xue-Ru Liu ◽

Yi-Ping Bai ◽

Jie Li ◽

...

Keyword(s):

Skeletal Muscle ◽

Lactic Acid ◽

Wnt Signaling ◽

Signaling Pathway ◽

Acid Production ◽

Lactic Acid Production ◽

Wnt Signaling Pathway ◽

Serum Levels ◽

Cross Sectional

Sepsis is a systemic inflammatory response syndrome resulting from infection. This study aimed at exploring the role of microRNA-140 (miR-140) in septic mice. Wnt family member 11 (WNT11) was verified to be a target gene of miR-140 after bioinformatic prediction and dual luciferase reporter gene assay. Importantly, miR-140 negatively regulated WNT11. We initially induced the model of sepsis by endotoxin, and then ectopic expression and knockdown experiments were performed to explore the functional role of miR-140 in sepsis. Additionally, cross-sectional areas of muscle fiber, lactic acid production, 3-methylhistidine (3-MH) and tyrosine (Tyr) production in extensor digitorium longus (EDL) muscles, and serum levels of inflammatory factors were examined. The effect of miR-140 on the expression of WNT signaling pathway-related and apoptosis-related factors in skeletal muscle tissue was determined. The experimental results indicated that upregulated miR-140 or silenced WNT11 increased cross-sectional areas of muscle fiber while decreasing lactic acid production, skeletal muscle cell apoptosis [corresponding to downregulated B cell lymphoma 2 (Bcl-2)-associated X protein (Bax) and caspase-3 and upregulated Bcl-2], and the proteolytic rate of Tyr and 3-MH. Also, overexpressed miR-140 or silenced WNT11 reduced inflammation as reflected by decreased serum levels of IL-6, IL-10, and TNF-α. Furthermore, overexpression of miR-140 was shown to suppress the activation of the WNT signaling pathway, accompanied by decreased expression of WNT11, β-catenin, and GSK-3β. Taken together, upregulation of miR-140 could potentially inhibit skeletal muscle lactate release, an indirect measure of glycolysis, and atrophy in septic mice through suppressing the WNT signaling pathway via inhibiting WNT11 expression.

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