scholarly journals Evaluation of Wild Segments Related to Growth Period Traits Using a Modified Wild Soybean (Glycine Soja Sieb. Et Zucc.) Chromosome Segment Substitution Line Population

2021 ◽  
Author(s):  
He Qingyuan ◽  
Shihua Xiang ◽  
Huawei Yang ◽  
Wubin Wang ◽  
Yingjie Shu ◽  
...  
Keyword(s):  
Glycine Soja ◽  
Wild Soybean ◽  
Growth Period ◽  
Mixed Linear Model ◽  
Single Nucleotide ◽  
Genetic Coefficient Of Variation ◽  
Varied Range ◽  
Segment Substitution ◽  
Genetic Coefficient ◽  
100 Seed Weight

Abstract Soybean seeds contain high levels of oil and protein providing 69% and 57% of dietary protein and oil respectively. Although many quantitative trait loci for 100-seed weight (100SW), protein content (PRC) and oil content (OIC) have been reported, their genetic controls in soybeans remain unclear. The QTL–allele constitution of three traits in the Sichuan and Chongqing eco-regions population (SCLBP) was studied using a representative sample composed of 228 accessions tested under four environments and analyzed based on 135 SSR and 107081 valid SNP (single nucleotide polymorphism linkage) markers. The varied range of 100SW, PRC and OIC of SCLBP were 4.82-33.35, 36.47-49.75 and 14.68-21.77 among accessions, respectively. The heritability (h2) and genetic coefficient of variation (GCV) of three traits were high. As a result, 26, 33 and 31 QTLs were found by SSR to be associated with 100SW, PRC and OIC, respectively. The allele of Sat_260 for 100SW was detected in 4 environments. In addition, 28, 198 and 250 loci for 100SW, PRC and OIC were found by SNP using mixed linear model (MLM), respectively. Further SNP haplotype analysis showed that 13, 35 and 60 blocks for 100SW, RPC and OIC were found. The block of Gm11_9895764-9917646 for 100SW was simultaneously detected in four environments. Among these QTLs, 1, 5, and 7 for 100SW, PRC and OIC were found by two methods of SSR and SNP at the same time. A majority of these QTLs overlapped with the previously reported loci. However, 9, 11 and 9 loci for 100SW, PRC and OIC using SSR and 3, 5 and 8 for 100SW, PRC and OIC haven’t been reported using SNP in the study, respectively. Moreover, the genes of Glyma.11g130800, Glyma.13g217000 and Glyma.08g122600 were considered to be the most likely genes controlling 100SW, PRC and OIC, respectively. Our findings provide evidence for mixed major plus polygenes inheritance for three traits and an extended understanding of their genetic architecture for molecular dissection and breeding utilization in soybeans.

scholarly journals The Evaluation of Agronomic Traits of Wild Soybean Accessions (Glycine soja Sieb. and Zucc.) in Heilongjiang Province, China

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 586
Author(s):  
Wei Li ◽  
Mu Peng ◽  
Zhen Wang ◽  
Yingdong Bi ◽  
Miao Liu ◽  
...  
Keyword(s):  
Quantitative Traits ◽  
Agronomic Traits ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Heilongjiang Province ◽  
Wild Type ◽  
The Rich ◽  
Geographical Distribution Pattern ◽  
100 Seed Weight

Wild soybean germplasm is distributed widely in China, particularly in Heilongjiang Province. In this study, 242 wild soybean accessions from four agricultural divisions in Heilongjiang Province were evaluated based on six qualitative and eight quantitative traits. Results showed that a large amount of variation occurred in these evaluated traits. Abundant qualitative traits included the wild type (78.51%), purple flowers (90.50%), needle leaf (39.26%), black seed (83.88%), brown hilum (52.07%), and mud film (87.60). Results of multivariate analysis based on quantitative traits showed that 100-seed weight, seeds weight per plant, number of seeds per plant, number of effective pods, and number of invalid pods were significantly different among samples (p < 0.05). A total of 27 germplasms were screened. Cluster analysis identified the 242 accessions into two groups, not following the geographical distribution pattern, with rich wild soybean resources revealed in the northern site. The present study indicated that wild soybean in Heilongjiang Province should be conserved in situ. The rich genetic diversity revealed in soybeans of different sites in Heilongjiang Province suggested its significant potential utilization in genetic improvement and breakthrough for soybean breeding. This information will help to exploit and conserve wild soybean accessions in Heilongjiang Province.

Phenotypic traits and diversity of different leaf shape accessions of the wild soybean (Glycine soja Sieb. et Zucc.) in China

2014 ◽  
Vol 94 (2) ◽  
pp. 397-404 ◽  
Author(s):  
Xuefei Yan ◽  
Hongkun Zhao ◽  
Xiaodong Liu ◽  
Qiyun Li ◽  
Yumin Wang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Seed Weight ◽  
Glycine Soja ◽  
Leaf Shape ◽  
Wild Soybean ◽  
Phenotypic Traits ◽  
Evolutionary Status ◽  
Important Indicator ◽  
Linear Leaf ◽  
100 Seed Weight

Yan, X., Zhao, H., Liu, X., Li, Q., Wang, Y., Yuan, C. and Dong, Y. 2014. Phenotypic traits and diversity of different leaf shape accessions of the wild soybean (Glycine soja Sieb. et Zucc.) in China. Can. J. Plant Sci. 94: 397–404. The phenotypic traits and diversity of different leaf shape groups of 6169 wild soybean accessions were investigated. The results included the following conclusions. First, the lanceolate leaf group had the highest genetic diversity. Second, the mean 100-seed weight was highest in the round leaf group (10.1 g); mean maturity time was longest in the eliptical leaf group (142 d); the ovate–round and linear leaf groups had higher mean protein content (45.7–46.1%); and the round leaf group had the highest oil content (16.3%). Finally, frequencies of white flower, high 100-seed weight and other traits in the round leaf group were disproportionately high; in contrast, linear leaf accessions were primarily characterized by purple flower and low 100-seed weight. Based on these results, we inferred that the lanceolate and linear leaves accessions tended to be wild types, while the round leaf group represented more recently evolved accessions. In addition, the accessions with eliptical, ovate–round and other leaves belonged to the intermediate evolutionary types. Our results also suggest that the abundant variation in leaf shape is an important indicator of level of genetic diversity and evolutionary status, which could facilitate the utilization of germplasm evaluation and classification for the wild soybean.

Development of a chromosome segment substitution line population with wild soybean (Glycine soja Sieb. et Zucc.) as donor parent

Euphytica ◽  
2012 ◽  
Vol 189 (2) ◽  
pp. 293-307 ◽  
Author(s):  
Wubin Wang ◽  
Qingyuan He ◽  
Hongyan Yang ◽  
Shihua Xiang ◽  
Tuanjie Zhao ◽  
...  
Keyword(s):  
Glycine Soja ◽  
Wild Soybean ◽  
Substitution Line ◽  
Chromosome Segment ◽  
Chromosome Segment Substitution Line ◽  
Donor Parent ◽  
Segment Substitution

scholarly journals Association between the Polymorphisms of fads2a and fads2b and Poly-Unsaturated Fatty Acids in Common Carp (Cyprinus carpio)

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1780
Author(s):  
Yan Zhang ◽  
Xiao-Qing Sun ◽  
Yu-Qing Ye ◽  
Qi Wang ◽  
Qing-Song Li ◽  
...  
Keyword(s):  
Common Carp ◽  
Unsaturated Fatty Acids ◽  
Major Gene ◽  
Fatty Acid Desaturase ◽  
Mixed Linear Model ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Food Fish ◽  
Rate Limiting ◽  
Fatty Acid Desaturase 2

Fatty acid desaturase 2 (fads2) is one of the rate-limiting enzymes in PUFAs biosynthesis. Compared with the diploid fish encoding one fads2, the allo-tetraploid common carp, one most important food fish, encodes two fads2 genes (fads2a and fads2b). The associations between the contents of different PUFAs and the polymorphisms of fads2a and fads2b have not been studied. The contents of 12 PUFAs in common carp individuals were measured, and the polymorphisms in the coding sequences of fads2a and fads2b were screened. We identified five coding single nucleotide polymorphisms (cSNPs) in fads2a and eleven cSNPs in fads2b. Using the mixed linear model and analysis of variance, a synonymous fads2a cSNP was significantly associated with the content of C20:3n-6. One non-synonymous fads2b cSNP (fads2b.751) and one synonymous fads2b cSNP (fads2b.1197) were associated with the contents of seven PUFAs and the contents of six PUFAs, respectively. The heterozygous genotypes in both loci were associated with higher contents than the homozygous genotypes. The fads2b.751 genotype explained more phenotype variation than the fads2b.1197 genotype. These two SNPs were distributed in one haplotype block and associated with the contents of five common PUFAs. These results suggested that fads2b might be the major gene responding to common carp PUFA contents and that fads.751 might be the main effect SNP. These cSNPs would be potential markers for future selection to improve the PUFA contents in common carp.

scholarly journals A Novel Pinkish-White Flower Color Variant Is Caused by a New Allele of Flower Color Gene W1 in Wild Soybean (Glycine soja)

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1001
Author(s):  
Jagadeesh Sundaramoorthy ◽  
Gyu Tae Park ◽  
Hyun Jo ◽  
Jeong-Dong Lee ◽  
Hak Soo Seo ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Substitution ◽  
Functional Activity ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Flower Color ◽  
Loss Of Function ◽  
Protein Variation ◽  
Color Variant ◽  
Color Gene

The enzyme flavonoid 3′,5′-hydroxylase (F3′5′H) plays an important role in producing anthocyanin pigments in soybean. Loss of function of the W1 locus encoding F3′5′H always produces white flowers. However, few color variations have been reported in wild soybean. In the present study, we isolated a new color variant of wild soybean accession (IT261811) with pinkish-white flowers. We found that the flower’s pinkish-white color is caused by w1-s3, a single recessive allele of W1. The SNP detected in the mutant caused amino acid substitution (A304S) in a highly conserved SRS4 domain of F3′5′H proteins. On the basis of the results of the protein variation effect analyzer (PROVEAN) tool, we suggest that this mutation may lead to hypofunctional F3′5′H activity rather than non-functional activity, which thereby results in its pinkish-white color.

Evaluation of seed amino acid content and its correlation network analysis in wild soybean (Glycine soja) germplasm in Japan

2021 ◽  
Vol 19 (1) ◽  
pp. 35-43
Author(s):  
Awatsaya Chotekajorn ◽  
Takuyu Hashiguchi ◽  
Masatsugu Hashiguchi ◽  
Hidenori Tanaka ◽  
Ryo Akashi
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Acid Content ◽  
Free Amino ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Amino Acid Content ◽  
Soybean Seeds ◽  
Free Amino Acid Content

AbstractWild soybean (Glycine soja) is a valuable genetic resource for soybean improvement. Seed composition profiles provide beneficial information for the effective conservation and utilization of wild soybeans. Therefore, this study aimed to assess the variation in free amino acid abundance in the seeds of wild soybean germplasm collected in Japan. The free amino acid content in the seeds from 316 accessions of wild soybean ranged from 0.965 to 5.987 mg/g seed dry weight (DW), representing a 6.2-fold difference. Three amino acids had the highest coefficient of variation (CV): asparagine (1.15), histidine (0.95) and glutamine (0.94). Arginine (0.775 mg/g DW) was the predominant amino acid in wild soybean seeds, whereas the least abundant seed amino acid was glutamine (0.008 mg/g DW). A correlation network revealed significant positive relationships among most amino acids. Wild soybean seeds from different regions of origin had significantly different levels of several amino acids. In addition, a significant correlation between latitude and longitude of the collection sites and the total free amino acid content of seeds was observed. Our study reports diverse phenotypic data on the free amino acid content in seeds of wild soybean resources collected from throughout Japan. This information will be useful in conservation programmes for Japanese wild soybean and for the selection of accessions with favourable characteristics in future legume crop improvement efforts.

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