scholarly journals Serum miR-218 is a Potential Biomarker in the Diagnosis of Colorectal Cancer

2020 ◽  
Author(s):  
Keqian Zhang ◽  
Tianqi Mao ◽  
Zhicheng He ◽  
Xiaojiao Wu ◽  
Yu Peng ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Roc Curve ◽  
Serum Samples ◽  
Chi Square ◽  
Diagnostic Significance ◽  
Serum Microrna ◽  
Potential Biomarker ◽  
Area Under Roc Curve ◽  
Chi Square Test ◽  
And Gender

Abstract Background: Our study was designed to explore the diagnostic role of serum microRNA-218 (miR-218) in colorectal cancer (CRC).Methods: Serum level of miR-218 was measured in 117 CRC samples and 88 normal controls using quantitative reverse transcription (qRT-PCR). Chi-square test was performed to assess the relationships between miR-218 expression and clinical characteristics of CRC patients. The receiver operating characteristic (ROC) analysis was established to investigate the diagnostic significance of miR-218 in CRC with the area under ROC curve (AUC).Results: MiR-218 was found to be weekly expressed in CRC serum samples compared with healthy controls (P < 0.001). And the down-regulation of miR-218 shared close relationships with lymph node metastasis (P = 0.013), vascular invasion (P = 0.020) and TNM stage (P = 0.031). However, age, tumor size and gender had no significant influence on miR-218 expression (P > 0.05). According to the ROC curve, miR-218 yielded an AUC value of 0.897 and an optimal cutoff point value of 0.021, providing a 83.8% sensitivity and a 78.4% specificity. Conclusion: In summary, decreased expression of serum miR-218 was detected in CRC patients and the expression of miR-218 was a diagnostic marker in CRC.

scholarly journals MiR-378 Has the Capacity to Serve as a Diagnostic Biomarker for Prostate Cancer Patient

2020 ◽  
Author(s):  
Shuangqing Cao ◽  
Lei Zheng
Keyword(s):  
Prostate Cancer ◽  
Roc Curve ◽  
Roc Analysis ◽  
Operating Characteristic ◽  
Diagnostic Value ◽  
Healthy Individuals ◽  
Chi Square ◽  
Urine Retention ◽  
Potential Biomarker ◽  
Chi Square Test

Abstract Background: This study aimed to examine the expression of serum miR-378 in prostate cancer (PCa) patients and healthy individuals and to identify the value of miR-378 in PCa diagnosis.Methods: The expression of serum miR-378 between groups was compared by t-test. The association between miR-378 expression and clinical characteristics of PCa patients was assessed using Chi-square test. The diagnostic value of serum miR-378 in PCa was estimated by the receiver operating characteristic (ROC) analysis.Results: The expression level of serum miR-378 in PCa patients was significantly lower than that in healthy individuals (P<0.0001). MiR-378 expression was affected by positive AR (P=0.004), large Gleason score (P=0.013) and advanced TNM stage (P=0.020), however, it had no relationship with age, serum PSA, NED rate and urine retention (all, P>0.05). The ROC curve showed that the optimal cutoff value was 1.845, giving the sensitivity and specificity of 75.21% and 89.77%, respectively. Besides, the area under the ROC curve (AUC) was 0.894, indicating serum miR-378 was of great diagnostic value in screening PCa patients from healthy controls (P<0.0001, 95%CI =0.852-0.936).Conclusions: Taken together, the increased expression of serum miR-378 might act as a potential biomarker for PCa diagnosis.

scholarly journals Per1 has the capacity to serve as a diagnostic biomarker for cholangiocarcinoma patient

2020 ◽  
Author(s):  
Ning Wang ◽  
Yanni Li ◽  
Yanfang Zheng ◽  
Huoming Chen ◽  
Xiaolong Wen ◽  
...  
Keyword(s):  
Roc Curve ◽  
Area Under The Curve ◽  
Serum Levels ◽  
Diagnostic Value ◽  
Positive Lymph Node ◽  
Early Screening ◽  
Chi Square ◽  
Potential Biomarker ◽  
Tumorigenesis And Progression ◽  
Chi Square Test

Abstract Background Period 1 (Per1) had been reported to be involved in the tumorigenesis and progression of human cancers. However, the clinical significance of Per1 in cholangiocarcinoma (CCA) was unclear. The purpose of this study was to explore the diagnostic value of serum Per1 in CCA patients. Methods Serum levels of Per1 in CCA patients and healthy individuals were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Chi-square test was used to evaluate the relationship between Per1 expression and clinical characteristics of patients. The diagnostic value of Per1 in CCA was estimated by establishing a receiver operating characteristic (ROC) curve. Results Serum Per1 level was significantly down-regulated in CCA patients compared to that in healthy controls (P < 0.001). Moreover, the decreased expression of Per1 was closely associated with poor histological differentiation (P = 0.040), advanced TNM stage (P = 0.035) and positive lymph node metastasis (P = 0.007). ROC curve indicated that the area under the curve (AUC) was 0.863 with a sensitivity of 88.1% and a specificity of 72.1%, revealing the high diagnostic value of serum Per1 in CCA. Conclusions Per1 is down-regulated in CCA and negatively correlated with tumor progression. Serum Per1 may be a potential biomarker for early screening of CCA.

scholarly journals High-expression of LncRNA MAFG-AS1 is associated with the prognostic of patients with colorectal cancer

2020 ◽  
Vol 66 (11) ◽  
pp. 1530-1535
Author(s):  
Weichun Cui ◽  
Yong Wang ◽  
Xiangji Shen ◽  
Xudong Wu ◽  
Hui Liu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Survival Time ◽  
Cox Regression ◽  
Disease Free Survival ◽  
High Expression ◽  
Rank Test ◽  
Depth Of Invasion ◽  
Chi Square ◽  
Potential Biomarker ◽  
Chi Square Test

SUMMARY OBJECTIVE: Long noncoding RNAs (lncRNAs) have been proven to exhibit distinct functions on the convoluted processes of tumor developments. Some studies on the biological functions of lncRNA MAFG-AS1 (MAFG-AS1) in cancers revealed that they may serve as an oncogene in some kinds of tumors, including colorectal cancer (CRC). However, little is known about the role of MAFG-AS1 in the prognostic of CRC. METHODS: A public dataset was mined for the screening of dysregulated lncRNAs in CRC. Quantitative real-time reverse transcription-polymerase chain reaction(qPCR) was used to compare the levels of MAFG-AS1 between paired MAFG-AS1 specimens and normal adjacent tissues. The correlations between MAFG-AS1 and clinic pathological features in CRC were analyzed using the chi-square test. The log-rank test and Kaplan-Meier test were carried out to compare the survival time of patients with high and low expressions of MAFG-AS1. Cox regression was applied for univariate and multivariate assays to validate whether MAFG-AS1 could be an independent factor in the prognosis of CRC. RESULTS: We found that the distinct upregulation of MAFG-AS1 in various tumors was a common event. MAFG-AS1 was distinctly up-regulated in CRC specimens compared to matched non-tumor specimens (p < 0.01). High MAFG-AS1 expressions were closely associated with depth of invasion (p = 0.011) and TNM stage (p = 0.022). Survival assays revealed that patients with high expression of MAFG-AS1 have a shorter overall survival (p = 0.0030) and disease-free survival (p = 0.0002). CONCLUSIONS: MAFG-AS1 can serve as a novel potential biomarker to predict CRC patients’ survival time.

scholarly journals HTATIP2 Represents an Innovative Bio-marker in Hepatocellular Carcinoma Detection

2020 ◽  
Author(s):  
Xianfeng Zhang ◽  
Xianjun Zhang ◽  
Xinguo Li ◽  
Hongbing Bao ◽  
Guang Li ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Serum Level ◽  
Roc Curve ◽  
Mrna Level ◽  
Venous Invasion ◽  
Operating Characteristics ◽  
Chi Square ◽  
Diagnostic Significance ◽  
Diagnostic Role ◽  
Chi Square Test

Abstract Background: This study was designed to investigate the serum level of HIV-1 Tat interactive protein 2 (HTATIP2) mRNA in hepatocellular carcinoma (HCC) patients and its diagnostic significance in the disease.Methods: The serum HTATIP2 mRNA level was determined by quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between HTATIP2 expression and clinical parameters was analyzed using Chi-square test. Receiver operating characteristics (ROC) curve was adopted to estimate the diagnostic role of serum HTATIP2 in HCC.Results: HCC patients showed a significantly lower serum level of HTATIP2 than the healthy control (P<0.001). The level of HTATIP2 was closely associated with venous invasion (P=0.011), lymph node metastasis (P=0.007) and TNM stage (P=0.016). ROC curve demonstrated that HTATIP2 could discriminate between HCC patients and healthy individuals at the optimal cutoff point of 2.39. Besides, the AUC was 0.892, with the corresponding sensitivity and specificity of 83.90% and 84.37%, respectively. Conclusions: HTATIP2 is negatively expressed in HCC and may be a diagnostic biomarker for this disease.

The Influence of Age and Gender on Mandibular Indices among the Libyan Population

2019 ◽  
Vol 3 (2) ◽  
Keyword(s):  
Negative Correlation ◽  
Bone Mineralization ◽  
Age Groups ◽  
T Test ◽  
Chi Square ◽  
Age And Gender ◽  
Significant Difference ◽  
Chi Square Test ◽  
And Gender ◽  
Libyan Population

Radiographic Mandibular Indices serve as easy and relatively cheap tools for evaluating bone mineralization. Objectives: To examine the effect of age and gender on three mandibular indices: the panoramic mandibular index (PMI), the mandibular ratio (MR) and the mandibular cortical index (MCI), among Libyan population. Methods: The three indices were measured on 317 digital (OPGs) of adult humans (155 males, 162 females). The sample was divided into six age groups (from 18-25 years through 56-65 years). The measurements were analyzed for interactions with age and sex, using SPSS (Statistical Package for Social Studies) software version no. 22. The tests employed were two way ANOVA, the unpaired T-test and chi-square test. Results: The mean PMI fluctuated between 0.37 s.d. 0.012 and 0.38 s.d. 0.012. among the sixth age groups. One-way ANOVA statistical test revealed no significant of age on PMI. On the other hand gender variation has effect on PMI, since independent sample t-test disclosed that the difference between the male and female PMI means statistically significant. ANOVA test showed that the means of MR among age groups showed a negative correlation i.e. MR mean declined from 3.01 in 18-25 age groups to 2.7 in 55-65 age groups. In contrary, the gender showed no effect on MR according two sample t-test at p> 0.05. In regards with MCI, statistical analysis showed that it affected by age that is C1 was decreasing by age while C2 and C3 were increased by age. Using chi square test the result indicated that there is a significant difference among the different age group and the two genders in MCI readings. Conclusion: PMI was influenced significantly by age but minimally by the gender. MR is not affected by gender but has a negative correlation with age. MCI is affected by both age and gender

scholarly journals Seroepidemiological study of toxoplasmosis in women referred to a pre-marriage counseling center in Alborz Province, Iran

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Melica Shahighi ◽  
Aliehsan Heidari ◽  
Hossein Keshavarz ◽  
Amir Bairami ◽  
Saeedeh Shojaee ◽  
...  
Keyword(s):  
Congenital Toxoplasmosis ◽  
Pcr Analysis ◽  
Women Of Childbearing Age ◽  
Childbearing Age ◽  
Serum Samples ◽  
Chi Square ◽  
Igg Avidity ◽  
Two Samples ◽  
Chi Square Test ◽  
Associated Risk Factors

Abstract Objectives The aim of the current study was to assess prevalence of Toxoplasma infection and its associated risk factors in women of childbearing-age in central Iran. Results Of 400 serum samples assessed for anti-T. gondii antibodies, 81 (20.25%) samples were positive for anti-T. gondii antibodies, including 74 positive samples (91.3%) for anti-T. gondii IgG and seven positive samples (8.7%) for IgG and IgM. Of seven IgG and IgM positive samples, five and two samples were high and low in IgG avidity, respectively. Based on PCR analysis, Toxoplasma infection was detected in one sample with anti-T. gondii IgM and low IgG avidity. The Chi-square test showed significant correlations of T. gondii seropositivity with history of undercooked meat consumption and contacts with cats (p < 0.05). In the present study, 79.75% of the participants were negative for IgG against T. gondii infection. Furthermore, recently acquired Toxoplasma infection was found using IgG avidity and PCR assays among women of childbearing-age in the study area, which would increase the risk of their fetus becoming infected. Educational program and antenatal screening of childbearing-age women for T. gondii infection may be important primary prevention strategies and help reduce the risk of congenital toxoplasmosis in this population.

scholarly journals Gender Differences in Witnessing and the Prevalence of Intimate Partner Violence from the Perspective of Children in Finland

2021 ◽  
Vol 18 (9) ◽  
pp. 4724
Author(s):  
Johanna Hietamäki ◽  
Marjukka Huttunen ◽  
Marita Husso
Keyword(s):  
Intimate Partner Violence ◽  
Partner Violence ◽  
Well Being ◽  
Intimate Partner ◽  
Clinical Samples ◽  
Chi Square ◽  
Child Victim ◽  
Chi Square Test ◽  
And Gender ◽  
Gender Variations

Background—Intimate partner violence (IPV) has both direct and longer-term effects on children’s well-being. Much of the research thus far has relied on caregiver reports of IPV and clinical samples of children. By contrast, minimal research has examined violence between parents from the perspective of children using nationwide samples. Objective—This study explored the frequency of IPV witnessed by children and gender variations regarding the victims, perpetrators, and witnesses. Methods—The data were derived from a sample of 11,364 children from the Finnish Child Victim Survey 2013. The children were between 11 and 17 years old and were enrolled in the Finnish school system. The main methods of analysis included crosstabulation and the chi-square test. Results—The results indicate that children witnessed more IPV against their mother (4.9%) than their father (3.5%). Girls reported having witnessed more violence against both their mother (7.0%) and father (5.1%) than boys did (mothers 2.7%, fathers 1.8%). Girls’ reports of IPV against both parents were twice or more than twice as common as boys’ reports. Conclusions—The above differences might result from gendered expectations and boys’ and girls’ different relationships to violence, as well as differences in the recognition and interpretation of violent incidents. Therefore, practitioners should adopt a gender-sensitive approach as a precondition and practice for working with children in social and health care.

scholarly journals Self‐Assessment Quiz Taking Behaviour Analysis in an Online Course

2016 ◽  
Vol 19 (2) ◽  
pp. 15-31 ◽  
Author(s):  
Yasin Ozarslan ◽  
Ozlem Ozan
Keyword(s):  
Online Course ◽  
Distance Learners ◽  
Self Assessment ◽  
Chi Square ◽  
Final Exam ◽  
Undergraduate Level ◽  
Chi Square Test ◽  
Learner Profile ◽  
Exam Scores ◽  
And Gender

AbstractSelf-assessment is vital for online learning since it is one of the most essential skills of distance learners. In this respect, the purpose of this study was to understand learners’ self-assessment quiz taking behaviours in an undergraduate level online course. We tried to figure out whether there is a relation between self-assessment quiz taking behaviours and final exam scores or not. In addition, we investigated how self-assessment quiz taking behaviour differs with respect to learner profile. In line with this purpose, 677 students’ 6092 test events across Project Culture course on Sakai CLE LMS were analyzed. For the analysis of the quantitative data, one-way ANOVA, Chi-Square test of independence, independent-samples t-test and descriptive statistics were utilized. The results revealed that learners who attended self-assessment quizzes regularly had higher final exam scores than others who did not attend those quizzes. Also, they were more satisfied with the course than others study field. In addition, learners who attended selfassessment quizzes regularly had a higher degree of perceived learning. However, number of attempts to those quizzes does not have an effect on final exam scores. On the other hand, a statistically significant relationship was found between attempt number and gender in favour of female learners.

scholarly journals Prevalence of Microdontia among Patients Undergoing Orthodontic Treatment

2020 ◽  
Keyword(s):  
Orthodontic Treatment ◽  
Cross Sectional Study ◽  
P Value ◽  
Chi Square ◽  
Cross Sectional ◽  
Single Tooth ◽  
Chi Square Test ◽  
Pre Treatment ◽  
And Gender ◽  
The Relationship

Objective: This study was carried out to determine the prevalence of microdontia among patients undergoing orthodontic treatment. Materials and Methods: This was a cross-sectional study conducted at Sindh Institute of Oral Health Sciences, (JSMU) from January-2020 to May-2020. Pre-treatment casts were taken of 140 subjects. The mesiodistal dimension of each tooth was recorded through the vernier caliper. Frequency and percentage were calculated for the presence of microdontia. The test applied was Pearson’s Chi-square test to assess the relationship between microdontia and variables like age and gender. P-value <0.05 was taken as statistically significant. Data analysis was performed on SPSS version 22. Results: A total of 140 subjects were selected i.e. 105 (75%) females and 35 (25%) males aged range 13 -30 years with mean age 18.29 ± 3.88. Out of 42, single tooth microdontia was found in 3 (7.1%), more than one tooth microdontia, and generalized microdontia was present in 36 (85.7%) and 3 (7.1%) respectively. Microdontia was found to be more common in the maxilla (n=42, 100%) than the mandible (n=14, 33.3%). It was found more common in females (n=37, 35.2%) as compared to males (n=5, 14.3%). Statistically significant relationship was found among gender and prevalence of microdontia (p=0.019) with a statistically insignificant relationship between age and presence of microdontia (p=0.228). Conclusions: Microdontia was found to be a frequent dental anomaly, was more common in maxilla and females with a significant association with gender.

scholarly journals The Influence of Different Factors on the Oral Health Status of Smoking and Nonsmoking Adults

2013 ◽  
Vol 14 (4) ◽  
pp. 731-737 ◽  
Author(s):  
Reem Saleem Tubaishat ◽  
Zain A Malkawi ◽  
Zakereyya S Albashaireh
Keyword(s):  
Health Status ◽  
Oral Health ◽  
Oral Health Status ◽  
Chi Square ◽  
Once Daily ◽  
Covering Letter ◽  
Gingival Bleeding ◽  
Chi Square Test ◽  
And Gender ◽  
Emotional Effects

ABSTRACT Aim The aim of this study to determine the influence of smoking on self-reported oral health status among university students. Materials and methods Voluntary sample of 669 adults of 18 to 26 years old were invited to participate in the study. They were asked to read a self-designated questionnaire and a covering letter which explained the purpose of the study. The questionnaire addressed self-reported demographics, oral hygiene and smoking habits; knowledge about smoking and its effects on oral health. Data were statistically analyzed using Chi-square test to determine statistically significant differences across the oral health status. Results The sample included 340 (56.7%) males and 240 (43.3%) females; 56.8% of the subjects were nonsmokers; 43.2% were current smokers, and 17.5% smoke argileh. Almost 24.7% of subjects started smoking because of emotional effects; 66.9% did not smoke because of its harm to health. The percentage of subjects with dental plaque on their teeth was 27.1%, and with calculus was 27.9%, and with stains was 26.9%. About 35.5% of the subjects brushed their teeth once daily. Statistically, significant association was proven between smoking and gender (p < 0.01); and smoking and halitosis (p < 0.01). Conclusion Smoking is significantly related to esthetics, calculus, tooth stains, halitosis, gingival bleeding, and gender with p < 0.01, p < 0.01, p < 0.01, p < 0.01, p = 0.05, and p < 0.01 respectively. How to cite this article Tubaishat RS, Malkawi ZA, Albashaireh ZS. The Influence of Different Factors on the Oral Health Status of Smoking and Nonsmoking Adults. J Contemp Dent Pract 2013;14(4):731-737.

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