scholarly journals PAZ Domain is Critical for PIWIL1 Function During Spermatogenesis in Chicken

2020 ◽  
Author(s):  
Qixin Guo ◽  
Lu Xu ◽  
Xiaoya Yuan ◽  
Yong Jiang ◽  
Hao Bai ◽  
...  
Keyword(s):  
Sperm Quality ◽  
3D Structure ◽  
Structure Model ◽  
Amino Acid Residues ◽  
Rt Pcr ◽  
Double Knockout ◽  
Stem Cell Proliferation ◽  
Cell Clones ◽  
Paz Domain

Abstract Background: Piwi-like protein 1 (PIWIL1) plays a crucial role in stem cell proliferation, embryogenesis, growth, and development. The aim of this study was to reveal the function of PIWIL1 and its PAZ (Piwi/Argonaute/Zwille) domain in chicken embryogenesis. Results: PIWI1 expression was analyzed in different stages of spermatogenesis by RT-PCR and the PAZ domain was mutated based on its 3D structure model using the CRISPR/Cas9 technology. The results indicated that PIWIL1 mRNA was specifically expressed in spermatogonium cells undergoing meiosis. After targeting the PAZ domain (300–370 amino acid residues), we obtained two mutant DF-1 cell clones with 23-bp and 8-bp deletions. Injection of the pCMV-Cas9-puro-sgRNA-2 construct into 2.5-day embryos resulted in generation of 19 different PAZ mutants (13 males and 6 females), which had delayed hatching, reduced quality of semen, and decreased expression of PIWIL1 and SOX2 at embryonic days 5 and 18. However, we could not obtain PAZ double knockout (KO) chickens by crossing of the F0 generation, suggesting that PAZ double KO may halt embryonic development. Conclusions: Our results indicate that PIWIL1 plays an important role in meiosis and that PAZ mutations can lead to decreased sperm quality, whereas its double KO may arrest embryogenesis in chicken.

Effect of new dietary supplement on sperm quality

2012 ◽  
Vol 153 (45) ◽  
pp. 1787-1792 ◽  
Author(s):  
Mária Horváth ◽  
Endre Czeizel
Keyword(s):  
Adverse Effect ◽  
Clinical Trial ◽  
Dietary Supplement ◽  
Male Fertility ◽  
Sperm Quality ◽  
Sperm Count ◽  
Before And After ◽  
The Usa ◽  
New Treatments

Introduction: There is a decline in male fertility thus new treatments are needed. Aims: To test the efficacy of a new dietary supplement developed in the USA and registered as a curing drug in Hungary (OGYI). Methods: In a clinical trial 100 men with low sperm quality (spermium count 5–20 M/ml, good motility 10–40%, and adverse shape 30–50%) were examined. Results: Sperm parameters were measured before and after a 3-month treatment and after another 3-month without treatment. This dietary supplement statistically and clinically significantly improved sperm count and motility. In 74 cases this dietary supplement demonstrated a beneficial effect on sperm quality (more than 10% increase in sperm count, or quality of motility, or shape); in 16 cases the improvement exceeded 30%. No adverse effect could be accounted for this treatment. Conclusions: This new dietary supplement may contribute to the treatment of male infertility. Orv. Hetil., 2012, 153, 1787–1792.

In Silico Prediction and Validation of Oxygen-Regulated Protein N-myc Downstream Regulated Gene 3 and Virtual Screening of Competitive Inhibitors of L-Lactate as Therapeutics

2019 ◽  
Vol 16 (6) ◽  
pp. 637-644
Author(s):  
Hongyu Cao ◽  
Yanhua Wu ◽  
Xingzhi Zhou ◽  
Xuefang Zheng ◽  
Ge Jiang
Keyword(s):  
Active Sites ◽  
Competitive Inhibition ◽  
Hydrophobic Effect ◽  
3D Structure ◽  
Amino Acid Residues ◽  
In Silico Prediction ◽  
Hypoxic Response ◽  
Drug Candidates ◽  
Competitive Inhibitors ◽  
Extracellular Regulated Protein Kinases

Background: N-myc downstream regulated gene 3 (NDRG3) is a newly discovered oxygen-regulated protein which will bind with L-Lactate in hypoxia and further activate Raf (rapidly accelerated fibrosarcoma)-ERK (extracellular regulated protein kinases) pathway, promoting cell growth and angiogenesis. Methods: Competitive inhibition on the binding of NDRG3 and L-Lactate may be potentially a useful strategy for the repression of hypoxic response mediated by NDRG3. The threedimensional (3D) structure of NDRG3 was built by using homology modeling for its crystal structure was not available. Then, L-Lactate was docked into NDRG3, from which we knew it bound with amino acid residues Gln69, His183, Asn189, Ala72 and Pro66 of NDRG3 in the most possible active sites. Approximately 3000 compounds have been virtually screened and the 6 topranked compounds were selected as reference molecules to analyze their interaction relationships, which illustrated that some of them might form electrostatic interaction with Glu70 and Asp187, π-&π stack with Phe75 and Tyr180, hydrogen bonds with Gly71 and Asn189, hydrophobic effect with Ala72 and Ile184. Results: Novel molecules were designed through structural optimization of the 6 top-ranked compounds and subsequently their ADMET properties were predicted. Conclusion: These molecules may be potential drug candidates for the suppression of hypoxic response mediated by NDRG3 and targeted therapy for hypoxia-induced diseases.

Free Testosterone Level And Quality of Cauda Epididymis Sperm of Local Rabbit That Given Commercial Feed Supplemented By Cod Fish Liver Oil

2016 ◽  
Vol 2 (3) ◽  
pp. 1
Author(s):  
Ni Gusti Ayu Manik Ermayanti ◽  
I Gusti Lanang Oka ◽  
I Gede Mahardika ◽  
I Putu Suyadnya
Keyword(s):  
Testosterone Level ◽  
Sperm Quality ◽  
Free Testosterone ◽  
Fish Liver ◽  
Testosterone Levels ◽  
Commercial Feed ◽  
One Way Anova ◽  
Free Testosterone Level ◽  
Range Test

This study was intended to determine the free testosterone levels and sperm quality of local rabbit that was given commercial feed supplemented cod fish liver oil.  The experiment design that was used in this research was Complete Random Design (CRD) with four experiments of feed, i.e. commercial feed without cod fish liver oil (R-0) as control, commercial feed + 1,5% cod fish liver oil (R-1), commercial feed + cod fish liver oil 3% (R-2), commercial feed + cod fish liver oil 4,5% (R-3). The each experiment included eight rabbits and feed experiment was given starting by 13 weeks to 26 weeks years old. The variable that observed was free testosterone level and sperm quality of local rabbit. The data that was obtained to be analyzed with One Way Anova and if its contrast was done more test with Duncan’s Multiple Range Test (DMRT). The result of this research was to show that supplementation of cod fish liver oil in commercial feed was to show the result that a real distinction of (P<0, 05) towards free testosterone level and sperm quality of local rabbit.

Influence of social status on growth performance, reproductive success and sperm quality of the African cichlid Aulonocara nyassae

2021 ◽  
Vol 237 ◽  
pp. 105292
Author(s):  
Walisson de Souza Silva ◽  
Lucas Pedro Gonçalves-Júnior ◽  
Marc Roger Jean Marie Henry ◽  
André Lima Ferreira ◽  
Isabela Fernanda Araújo Torres ◽  
...  
Keyword(s):  
Reproductive Success ◽  
Growth Performance ◽  
Social Status ◽  
Sperm Quality ◽  
African Cichlid

The clpB gene of Bifidobacterium breve UCC 2003: transcriptional analysis and first insights into stress induction

Microbiology ◽  
2005 ◽  
Vol 151 (9) ◽  
pp. 2861-2872 ◽  
Author(s):  
Marco Ventura ◽  
John G. Kenny ◽  
Ziding Zhang ◽  
Gerald F. Fitzgerald ◽  
Douwe van Sinderen
Keyword(s):  
3D Structure ◽  
Binding Mode ◽  
Dna Interaction ◽  
Transcriptional Analysis ◽  
Amino Acid Residues ◽  
Mobility Shift ◽  
Bifidobacterium Breve ◽  
Gel Mobility Shift ◽  
Slot Blot Analysis

The so-called clp genes, which encode components of the Clp proteolytic complex, are widespread among bacteria. The Bifidobacterium breve UCC 2003 genome contains a clpB gene with significant homology to predicted clpB genes from other members of the Actinobacteridae group. The heat- and osmotic-inducibility of the B. breve UCC 2003 clpB homologue was verified by slot-blot analysis, while Northern blot and primer extension analyses showed that the clpB gene is transcribed as a monocistronic unit with a single promoter. The role of a hspR homologue, known to control the regulation of clpB and dnaK gene expression in other high G+C content bacteria was investigated by gel mobility shift assays. Moreover the predicted 3D structure of HspR provides further insight into the binding mode of this protein to the clpB promoter region, and highlights the key amino acid residues believed to be involved in the protein–DNA interaction.

scholarly journals Investigation of major amino acid residues of anti-norfloxacin monoclonal antibodies responsible for binding with fluoroquinolones

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Patamalai Boonserm ◽  
Songchan Puthong ◽  
Thanaporn Wichai ◽  
Sajee Noitang ◽  
Pongsak Khunrae ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Amino Acid ◽  
3D Structure ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Amino Acid Residues ◽  
Variable Regions ◽  
Complementarity Determining Regions ◽  
Crucial Part ◽  
Major Amino Acid

AbstractIt is important to understand the amino acid residues that govern the properties of the binding between antibodies and ligands. We studied the binding of two anti-norfloxacins, anti-nor 132 and anti-nor 155, and the fluoroquinolones norfloxacin, enrofloxacin, ciprofloxacin, and ofloxacin. Binding cross-reactivities tested by an indirect competitive enzyme-linked immunosorbent assay indicated that anti-nor 132 (22–100%) had a broader range of cross-reactivity than anti-nor 155 (62–100%). These cross-reactivities correlated with variations in the numbers of interacting amino acid residues and their positions. Molecular docking was employed to investigate the molecular interactions between the fluoroquinolones and the monoclonal antibodies. Homology models of the heavy chain and light chain variable regions of each mAb 3D structure were docked with the fluoroquinolones targeting the crucial part of the complementarity-determining regions. The fluoroquinolone binding site of anti-nor 155 was a region of the HCDR3 and LCDR3 loops in which hydrogen bonds were formed with TYR (H:35), ASN (H:101), LYS (H:106), ASN (L:92), and ASN (L:93). These regions were further away in anti-nor 132 and could not contact the fluoroquinolones. Another binding region consisting of HIS (L:38) and ASP (H:100) was found for norfloxacin, enrofloxacin, and ciprofloxacin, whereas only ASP (H:100) was found for ofloxacin.

The Potential Effect of Mucuna pruriens Seed Extract on Sperm Quality experimental study on mice exposed to cigarette smoke

2021 ◽  
Vol 20 (4) ◽  
pp. 768-773
Author(s):  
Meidona Nurul Milla ◽  
Yani Istadi ◽  
Vania Shaula ◽  
Deastri Anjeas Wari ◽  
Chntyia Dwi Cahyani Puspitasari ◽  
...  
Keyword(s):  
Experimental Study ◽  
Cigarette Smoke ◽  
Sperm Quality ◽  
Medical Science ◽  
Seed Extract ◽  
Reproductive Age ◽  
Testicular Atrophy ◽  
Mucuna Pruriens ◽  
Control Group

Background: Infertility has been more common problems among couple of reproductive age. One of the factors causing this disorder is unhealthy environmental factors including exposure to cigarette smoke. Polynuclear aromatic hydrocarbons (PAHs) in cigarette smoke can cause testicular atrophy, while the free radicals can inhibit the stages of spermatogenesis, and nicotine in cigarettes affects the brain dopamine levels affecting the levels of GnRH, and subsequently affect the levels of FSH and LH needed in spermatogenesis. The use of Mucuna pruriens seed extract containing antioxidants and L-dopa is expected to improve the quality of sperm after exposure to cigarette smoke. Objective: This study aimed to determine the effect of Mucuna pruriens seed extract on the sperm quality in mice exposed to cigarette smoke. Methods: This study was an experimental study with a post test only control group design. A total of 20 mice were divided into 4 groups of five mice each. All groups were exposed to cigarette smoke. Group 1 was the negative control exposed to cigarette smoke. Groups 2, 3, 4 were exposed to cigarettes smoke and given Mucuna pruriens seed extracts at the dose of 250; 300; and 350 mg/Kg BW/day. Parameters of sperm quality included concentration, morphology, motility and viability. Results: Post hoc tests showed there were significant differences among treatment groups. Conclusion: the administration of Mucuna pruriens seed extract affects the sperm quality of BALB/c mice exposed to cigarettes smoke. Bangladesh Journal of Medical Science Vol.20(4) 2021 p.768-773

Addition of l-carnitine to the freezing extender improves post-thaw sperm quality of Okinawan native Agu pig

2021 ◽  
Author(s):  
Hideki Tatemoto ◽  
Naoto Osokoshi ◽  
Moe Hirai ◽  
Yusuke Masuda ◽  
Toshihiro Konno ◽  
...  
Keyword(s):  
Sperm Quality
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