Levofloxacin Might be Safe to use for OSCC Patients

Abstract PurposeSince oral squamous cell carcinoma (OSCC) patients are exhausted against the powerful chemotherapies and radiotherapies after surgeries, therefore most of the studies still look for less toxic but effective alternatives with new ideas such as antibiotic combinations.MethodsThe antiproliferative and apoptotic outcomes of levofloxacin with cisplatin combination as well as their single usage were examined with WST-1, Caspase-3/BCA and Annexin-V methods on SCC-15 cells and on a healthy cell line (MRC-5).Results24h treatment of 50 mM single levofloxacin, 50 mM single cisplatin and 50 mM levofloxacin-cisplatin combination resulted in cell viability rates of SCC-15 cells as 90%, 67% and 80.8% respectively. Caspase-3 enzyme activity was enhanced 0.92-fold for single levofloxacin, 13.05-fold for single cisplatin and 9.73-fold for the combination of levofloxacin-cisplatin, the total apoptotic activity of single levofloxacin, single cisplatin and levofloxacin-cisplatin combination were observed as 4.88%, 21.14% and 16.21% respectively on SCC-15. Also MRC-5 were showed the lower toxicity than cancer cells via apoptosis.ConclusionLevofloxacin-cisplatin combination results have also ended up apoptotic results with less toxicity for cells than single cisplatin treatment. Therefore, our apoptotic findings suggest that the different dosage combinations with levofloxacin and cisplatin are necessary to understand the interaction for the treatment of tongue squamous cell carcinoma.

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C-reactive protein promotes tongue squamous cell carcinoma chemoresistance by inhibiting the activation of caspase-3/9 via the CD64/AKT/mTOR pathway

Human Cell ◽

10.1007/s13577-021-00555-7 ◽

2021 ◽

Author(s):

Xiaodong Yan ◽

Meng Cao ◽

Zhigang Wang ◽

Shenglin Wang ◽

Qinchao Chen

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Caspase 3 ◽

Mtor Pathway ◽

Tongue Squamous Cell Carcinoma ◽

C Reactive Protein ◽

Reactive Protein

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DIAPH1 Is Upregulated and Inhibits Cell Apoptosis through ATR/p53/Caspase-3 Signaling Pathway in Laryngeal Squamous Cell Carcinoma

Disease Markers ◽

10.1155/2019/6716472 ◽

2019 ◽

Vol 2019 ◽

pp. 1-10 ◽

Cited By ~ 3

Author(s):

Jiechao Yang ◽

Liang Zhou ◽

Yanping Zhang ◽

Juan Zheng ◽

Jian Zhou ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Western Blot ◽

Cell Carcinoma ◽

Squamous Cell ◽

Blot Analysis ◽

Western Blot Analysis ◽

Molecular Mechanisms ◽

Caspase 3 ◽

Laryngeal Squamous Cell Carcinoma ◽

Poor Overall Survival

Cancer bioinformatics has been used to screen possible key cancer genes and pathways. Here, through bioinformatics analysis, we found that high expression of diaphanous related formin 1 (DIAPH1) was associated with poor overall survival in head and neck squamous cell carcinoma and laryngeal squamous cell carcinoma (LSCC). The effect of DIAPH1 in LSCC has not been previously investigated. Therefore, we evaluated the expression, function, and molecular mechanisms of DIAPH1 in LSCC. Immunohistochemistry and western blot analysis confirmed the significant upregulation of DIAPH1 in LSCC. We used DIAPH1 RNA interference to construct two DIAPH1-knockdown LSCC cell lines, AMC-HN-8 and FD-LSC-1, and validated the knockdown efficiency. Flow cytometry data showed that DIAPH1 inhibited apoptosis. Further, western blot analysis revealed that DIAPH1 knockdown increased the protein levels of ATR, p-p53, Bax, and cleaved caspase-3, -8, and -9. Thus, DIAPH1 is upregulated in LSCC and may act as an oncogene by inhibiting apoptosis through the ATR/p53/caspase-3 pathway in LSCC cells.

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Bone Marrow Mesenchymal Stem Cells (BMSCs) with High Expression miR-155 Affect the Proliferation and Metastasis of Oral Squamous Cell Carcinoma by Regulating Phosphatase and Tensin Homolog 12 (PTEN12)

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2021.2705 ◽

2021 ◽

Vol 11 (8) ◽

pp. 1571-1575

Author(s):

Guowu Ma ◽

Jia Hou ◽

Jiezi Qiu ◽

Jianlin Fan ◽

Jianxin Yang

Keyword(s):

Cell Proliferation ◽

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

High Expression ◽

Tensin Homolog ◽

Proliferation And Metastasis ◽

Apoptotic Activity ◽

And Migration

Oral squamous cell carcinoma (OSCC) had the poor prognosis. miR-155 was involved in some diseases. However, whether BMSCs with high expression miR-155 affect the proliferation and metastasis of OSCC is unclear. Our study aims to assess BMSCs’ effect on OSCC cells. miR-155 level in OSCC tumor tissues was analyzed. BMSCs were transfected with miR-155 followed by analysis of cell proliferation by MTT assay, cell apoptosis and migration, and MMP-9 level by ELISA and PTEN12 level. In the tumor tissue, miR-155 level was significantly increased (P <0.05). Co-culture of BMSCs with high expression miR-155 with OSCC cells could significantly promote OSCC cell proliferation and reduced cell apoptotic activity, increased cell migration and MMP-9 secretion as well as downregualted PTEN12 expression (P <0.05). In conclusion, miR-155 was increased in the OSCC patients and BMSCs of high expression miR-155 could promote the proliferation and metastasis of OSCC by regulating PTEN12.

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Increased killing of SCCVII squamous cell carcinoma cells after the combination of Pc 4 photodynamic therapy and dasatinib is associated with enhanced caspase-3 activity and ceramide synthase 1 upregulation

International Journal of Oncology ◽

10.3892/ijo.2013.2132 ◽

2013 ◽

Vol 43 (6) ◽

pp. 2064-2072 ◽

Cited By ~ 12

Author(s):

DUSKA SEPAROVIC ◽

PAUL BREEN ◽

NITHIN B. BOPPANA ◽

ERIC VAN BUREN ◽

NICHOLAS JOSEPH ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Photodynamic Therapy ◽

Cell Carcinoma ◽

Squamous Cell ◽

Caspase 3 ◽

Carcinoma Cells ◽

Ceramide Synthase

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Expression levels of cleaved caspase-3 and caspase-3 in tumorigenesis and prognosis of oral tongue squamous cell carcinoma

PLoS ONE ◽

10.1371/journal.pone.0180620 ◽

2017 ◽

Vol 12 (7) ◽

pp. e0180620 ◽

Cited By ~ 22

Author(s):

Pei-Feng Liu ◽

Yu-Chang Hu ◽

Bor-Hwang Kang ◽

Yu-Kai Tseng ◽

Pi-Chuang Wu ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Caspase 3 ◽

Tongue Squamous Cell Carcinoma ◽

Oral Tongue ◽

Expression Levels

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The microbial characteristics of patients with esophageal squamous cell carcinoma before and after immunotherapy.

Journal of Clinical Oncology ◽

10.1200/jco.2021.39.15_suppl.e16016 ◽

2021 ◽

Vol 39 (15_suppl) ◽

pp. e16016-e16016

Author(s):

Jing Zuo ◽

Wenjing Lv ◽

Yudong Wang ◽

Zhisong Fan ◽

Li Feng ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

16S Rrna ◽

Esophageal Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Fecal Samples ◽

Β Diversity ◽

Α Diversity ◽

Before And After ◽

New Ideas

e16016 Background: Esophageal squamous cell carcinoma (ESCC) is a common malignancy without effective therapy. Immune checkpoint–oriented immunotherapies have shown considerable promise and the advent of esophageal microbiome provides researchers with new ideas. Methods: DNA was extracted from blood, oral mucosal, saliva, urine, fecal samples from 20 ESCC patients before and after immunotherapy. Total microbial genomic DNA samples were extracted using an OMEGA Soil DNA Kit (D5625-01). The V3–V4 regions of bacterial 16S rRNA genes were amplified by PCR using the forward primer and the reverse primer and were sequenced with Illumina MiSeq platform. In order to comprehensively evaluate the α diversity of microbial communities, we used Chao1 and Observed Species indices to characterize the richness, Shannon and Simpson indices to characterize the diversity. PCoA were used to analyze differences in β diversity. Functions of 16S rRNA sequences were predicted using the PICRUSt2 and KEGG databases. Results: A comparison of blood, oral mucosal, saliva, urine, fecal samples of ESCC patients before and after immunotherapy showed that α diversity was not statistically significant. In terms of β diversity, no statistically significant differences were detected within blood, oral mucosal, saliva, urine, fecal samples of ESCC patients before and after immunotherapy. In ESCC patients treated before immunotherapy, the α diversity and β diversity of blood, oral mucosal, saliva, urine, fecal samples were different, and in ESCC patients treated after immunotherapy had the same rule. At the phylum level, the top 5 microbes in ESCC patients before and after immunotherapy were Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Fusobacteria. At the genus level, the top 5 microbes in ESCC patients before immunotherapy were Aquabacterium, Streptococcus, Prevotella, Veillonella, Bacteroides, and in ESCC patients after immunotherapy were Aquabacterium, Streptococcus, Prevotella, Faecalibacterium, Veillonella. In terms of the microbial functions in ESCC patients before and after immunotherapy, the metabolic pathways accounted for the most. Conclusions: This study is conducive to exploring new mechanisms for tumor cells to evade host immune surveillance, providing new ideas and new strategies for the microecology-based immunotherapy of ESCC.

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