scholarly journals Impact of Manipulating Quail Eggs with Betaine During Early Embryogenesis on Chick Development, Hatchability, Hematological Variables, and Some Physiological Estimates

2021 ◽  
Author(s):  
Ahmed Abdelkareem Abuoghaba ◽  
Fatma Ali ◽  
Amira Ahmed Mohamed Abdelwahab
Keyword(s):  
Incubation Temperature ◽  
Positive Control ◽  
Negative Control ◽  
Early Embryogenesis ◽  
Feed Consumption ◽  
Blood Protein ◽  
Distilled Water ◽  
Slaughter Weight ◽  
Japanese Quails ◽  
Chick Development

Abstract This study evaluated the effect of spraying incubated quail eggs with betaine on chick development, hatchability and some physiological estimates of Japanese quails during early embryogenesis. A total of 750 eggs were equally divided into two groups (2 groups × 5 treatments × 5 replicates × 15 eggs). Eggs in the 1st group were incubated at normal incubation temperature (37.5°C/NIT), while those in the 2nd group were incubated at chronic incubation temperature (39.5 °C/CIT) for 3 hours daily from the 4th up to and 6th day of incubation. Eggs in NIT and CIT groups were subjected to five treatments, T1 (negative control), T2 sprayed distilled water (positive control), while T3, T4, T5 groups sprayed distilled water supplemented with 500, 1000 and 2000mg betaine/L respectively. The chick weight at hatch and slaughter weight and first egg weight was significantly impaired by CIT treatment. CIT group revealed a significant increase in the H/L ratio and a significant decrease in T3 hormone and blood protein levels than the NIT group. Regarding betaine effects, the embryonic mortality rates, hatchability, hatched chick weight, and slaughter weight were significantly improved compared with the control. Also, betaine significantly increased blood protein and T3 hormone levels and significantly decrease the liver enzymes levels and total feed consumption compared with untreated group. The RV/TV ratio of quails in CIT group was significantly increased, while betaine treatment significantly decreased this ratio. Considering these results, it’s strongly suggested that spraying of betaine on eggs at 1000 or 2000 mg//L optimizes Japanese quails performance.

scholarly journals Silver Nanoparticle of Acalypha indica Linn. Leaf As Bio-larvicide against Anopheles sp. Larvae

2021 ◽  
Vol 9 (A) ◽  
pp. 760-765
Author(s):  
Yos Banne ◽  
Olfie Sahelangi ◽  
Steven Soenjono ◽  
Elisabeth Natalia Barung ◽  
Selfie Ulaen ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Group ◽  
Distilled Water ◽  
Lc50 Value ◽  
Acalypha Indica

BACKGROUND: Acalypha indica Linn. has been used as traditional medicine, it contains flavonoids, alkaloids, tannins, saponins, steroids, triterpenoids, and essential oils. AIM: This study aimed to determine the bio-larvicide effects of A. indica Linn. leaf stew and the silver nanoparticles against Anopheles sp. larvae. METHODS: The fresh leaves of A. indica Linn. extracted using distilled water at 100°C for 30 min. The silver nanoparticles were made by mixing a solution of silver nitrate with the stew, which acts as a reducing agent. The resulting silver nanoparticles were characterized by particle size analyzer and UV-vis spectrophotometer. The bio-larvicide effects against Anopheles sp. larvae performed using a completely randomized design. There were eight groups consisted of ten larvae and three replications. Treatment groups of stew and silver nanoparticle for concentrations 0.05%, 0.5%, and 5%, respectively. The negative control group was distilled water and the positive control group was the 0.01% abate solution. Assessment of larvicide activity was carried out every hour for 6 h and continued if there were larvae that live up to 24 h. The LC50 value was calculated based on Probit analysis. RESULTS: The results showed that the A. indica Linn. leaf stew can be made into silver nanoparticles preparations, optimal results were obtained from a mixture of 1% stew and 3 mM AgNO3. The result of bio-larvicides effect test against Anopheles sp. larvae showed that the LC50 value of the A. indica Linn. leaf stew was 727,3 ppm and the LC50 value of silver nanoparticles was 3.366 ppm. CONCLUSION: It can be concluded that A. indica Linn. is a promising larvicidal plant and can be made into silver nanoparticle preparations.

scholarly journals Pengaruh Antibakteri Ekstrak Kulit Batang Matoa (Pometia pinnata) terhadap Bakteri Staphylococcus aureus secara In vitro

Jurnal MIPA ◽  
2013 ◽  
Vol 2 (2) ◽  
pp. 128 ◽  
Author(s):  
Mercy Ngajow ◽  
Jemmy Abidjulu ◽  
Vanda S. Kamu
Keyword(s):  
Staphylococcus Aureus ◽  
Secondary Metabolites ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Positive Control ◽  
Negative Control ◽  
Bark Extract ◽  
Distilled Water

Telah dilakukan penelitian secara kualitatif terhadap pengaruh antibakteri dari ekstrak kulit batang matoa (Pometia pinnata. Spp.) terhadap bakteri Gram positif Staphylococcus aureus . Sebelum dilakukan uji antibakteri, sampel yang telah diekstrak secara maserasi diuji fitokimia terlebih dahulu untuk menentukan kandungan metabolit sekunder yang telah diketahui berperan sebagai agen antibakteri. Setelah dilakukan uji fitokimia, ekstrak diuji aktivitas antibakterinya terhadap bakteri Staphylococcus aureus dengan menggunakan teknik difusi agar dengan cara sumuran. Ekstrak dilarutkan pada aquades steril dengan perbandingan 2 g ekstrak pada 2 mL air. Untuk kontrol positif, digunakan ciprofloxacin dan aquades steril sebagai kontrol negatif. Hasil penelitian ini menunjukkan bahwa ekstrak kulit batang matoa memiliki aktivitas antibakteri setelah diinkubasi selama 24 jam pada 37 OC. Dari 3 kali pengulangan dengan masing – masing 3 sumuran, didapat zona hambat masing – masing 16.84 mm, 12.5 mm dan 14.5 mm dengan kontrol positif 29.67 mm serta kontrol negatif 0 mm. Hasil yang diperoleh didukung oleh keberadaan metabolit sekunder hasil uji fitokimia yaitu tanin, flavonoid, terpenoid dan saponin.A qualitative study has done  of the antibacterial effect of matoa (Pometia pinnata) stem bark extract against Gram-positive bacteria Staphylococcus aureus . Before the antibacterial test , samples were extracted by maceration and then  phytochemical  tested to measuring the content of secondary metabolites  that have been known to act as an antibacterial agent . After being tested of phytochemical , extracts were tested the antibacterial effect  against Staphylococcus aureus using agar diffusion technique. Extract was dissolved in sterile distilled water with a ratio of 2 g of extract in 2 mL of water . For the positive control , use of ciprofloxacin and sterile distilled water as a negative control . Results of this study indicate that matoa bark extract has antibacterial effect after incubation for 24 h at 37OC . Of 3 times with each repetition - each 3 wells, the inhibition zone obtained - each 16.84 mm , 12.5 mm and 14.5 mm with 29.67 mm of positive control and a negative control by 0 mm . The results are supported by the presence of secondary metabolites by phytochemical test such as tannins , flavonoids , terpenoids and saponins.

scholarly journals Effects of coronal leakage on concentration of hydrogen ions and calcium release of several calcium hydroxide pastes over different periods of time

2013 ◽  
Vol 1 (3) ◽  
pp. 230
Author(s):  
Mariana Pires Crespo ◽  
Milton Carlos Kuga ◽  
Marcus Vinicius Reis Só ◽  
Katia Cristina Keine ◽  
André Luis Shinohara
Keyword(s):  
Calcium Hydroxide ◽  
Calcium Release ◽  
Water Concentration ◽  
Positive Control ◽  
Negative Control ◽  
Distilled Water ◽  
Filling Material ◽  
Hydrogen Ions ◽  
Ion Release ◽  
Temporary Filling

PURPOSE: To evaluate the effects of coronal leakage on concentration of hydrogen ions (pH) and calcium release of several calcium hydroxide pastes, over different periods of time. MATERIAL AND METHODS:  Fifty extracted human mandibular central incisors (n=10) were instrumented up to the F2 instrument and assigned to the following intracanal dressing: G1- Calen, G2- Calen with 0.4% chlorhexidine (CHX), G3- Calcium hydroxide with camphorated paramonochlorophenol (CPMC) and glycerin, G4- Calen, but temporary filling material maintained during all test (positive control) and G5- Root canal without intracanal dressing (negative control). All groups were immersed in distilled water for 7 days. In sequence, the temporary filling materials were removed, except in controls groups. All specimens were individually mounted on a specific device and only its root again immersed in distilled water. Concentration of hydrogen ions and calcium release by calcium hydroxide pastes in distilled water were evaluated in 24h, 7, 14 and 28 days. The results were submitted to ANOVA test (p = 0.05). After 28 days, root canals from experimental groups were examined in SEM. RESULTS: G1, G2, G3 and G4 presented similar pH values and calcium release and did not differ from each other (p>0.05), up to 7 days. After this time G1, G2 and G3 presented values lower values than G4 (p<0.05). In SEM analysis, calcium hydroxide residues were observed in all experimental groups. CONCLUSIONS: After 7 days, coronal leakage decreased the concentration of hydrogen ions and calcium ion release provided by all calcium hydroxide pastes.

scholarly journals Penggunaan Kulit Nanas Fermentasi dalam Ransum yang Mengandung Gulma Berkhasiat Obat Terhadap Lemak dan Kolesterol Ayam Broiler

2015 ◽  
Vol 15 (1) ◽  
pp. 20-27 ◽  
Author(s):  
Wasir Ibrahim ◽  
Rita Mutia ◽  
Nurhayati Nurhayati
Keyword(s):  
Broiler Chicken ◽  
Liver Weight ◽  
Positive Control ◽  
Negative Control ◽  
Blood Cholesterol ◽  
Feed Consumption ◽  
Randomized Design ◽  
Pineapple Peel ◽  
Fat Level ◽  
Range Test

(Use of Fermented Pineapple Peel in the Ration Containing Medicinal Weeds on Fat and Cholesterol of Broiler Chicken) ABSTRACT. The objective of the experiment was to determine the effect of using fermented pineapple peel in the ration containing medicinal weeds on fat and cholesterol broiler chicken. The experiment was designed into completely randomized design (CRD) with 5 treatments and 4 replicates. Treatments consisted of P0 = 0% of fermented pineapple peels meal (FPPM) in ration containing 0% medicinal weed (MW), positive control). P1 = 0% of fermented pineapple peels meal (FPPM) in the ration containing 2 % medicinal weed (MW), negative control). P2 = 7,5% of FPPM in the ration containing P1. P3 = 15% of FPPM in the ration containing P1. P4 = 22,5% of FPPM in the ration containing P1. Two hundred 2 days old male broiler chicken were used in this study and kept for 42 days. The parameters were feed consumption, liver weight, bile volume, meat fat level, and meat and blood cholesterol. The observed data were analyzed by Statistical Product and Service Solution (SPSS 16.0) and significant effect among treatments were tested by Duncan’s Multiple Range Test. The results of thus study showed that the treatments were significant effect (P 0.05) on  feed consumption, meat fat of thigh. but were not significant effect (P 0.05) on liver weight, bile volume, meat fat level and meat and blood cholesterol. It is concluded  that use of fermented pineapple peel in the ration improved fat and cholesterol on broiler chicken.

scholarly journals CONTROL OF Vibrio alginolyticus INFECTION IN ASIAN SEA BASS Lates calcarifer USING AMBON BANANA PLANT POWDER Musa paradisiacal THROUGH THE FEED

2021 ◽  
Vol 15 (2) ◽  
pp. 85
Author(s):  
Hamida Pattah ◽  
Dinamella Wahjuningrum ◽  
Munti Yuhana ◽  
Widanarni Widanarni
Keyword(s):  
Challenge Test ◽  
Sea Bass ◽  
Positive Control ◽  
Negative Control ◽  
Vibrio Alginolyticus ◽  
Feed Consumption ◽  
Banana Plant ◽  
Asian Sea Bass ◽  
Antibiotic Control ◽  
Lower Production

Asian sea bass is one of economically important commodities in aquaculture. However, its culture often challenged by vibriosis infection which resulted in lower production. The study aimed to evaluate the effectiveness of the ambon banana plant powder application in feed as an effort to control the disease caused by Vibrio alginolyticus infection. Asian sea bass with total length of 7.48 ± 0.45 cm. Five different treatments were applied in this experiment, i.e. positive control, negative control, prevention, curative, and antibiotic application. The challenge test is carried out on the 15th day. Challenge test was performed with intramuscularly injection of V. alginolyticus at the cell density of 106 CFU/fish. The results showed the amount of feed consumption after the challenge test was highest in the curative and antibiotic control. However, these results were not significantly different (P>0.05) among preventive and curative treatments but were significantly different from controls (P<0.05). Fish treated with supplemented feed showed the highest total erythrocytes, total leukocytes, hemoglobin, respiratory burst, and lysozyme activity compared to controls. IL-1b gene expression increased after the challenge test with the highest level of expression in the curative treatment. It can be concluded that the administration of ambon banana plant powder (3 g/100 g diet) could control of V. alginolyticus infection in Asian sea bass and resulted 70.00% survival rate on prevention and 83.33% on curative treatments.

scholarly journals UJI EFEK ANTIPIRETIK EKSTRAK MENIRAN (Phyllantus niruri L.) PADA TIKUS WISTAR (Rattus norvegicus) JANTAN YANG DIINDUKSI VAKSIN DPT-HB

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Ivana Jansen ◽  
Jane Wuisan ◽  
Henoch Awaloei
Keyword(s):  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Distilled Water ◽  
Phyllanthus Niruri ◽  
Antipyretic Effect ◽  
Flower Extract ◽  
Hb Vaccine

Abstract: The aim of this study is to investigate the antipyretic effect of leaf-flower extract (Phyllanthus niruri L.) in Wistar rats induced by DPT-HB vaccine. Samples were 15 Wistar rats as test animals and were divided into five groups of three in each. The negative control group was given orally distilled water, positive control group was given paracetamol and 3 experimental groups were given ethanolic leaf-flower extracts (100 mg, 200 mg and 300 mg /200 g body weight of rats). Pyrexia was induced in rats by intramuscular injection of 0.2 ml DPT-HB vaccine. Antipyretic activity was measured at 0, 30, 60, 90, 120, 150, 180 minutes after administration of leaf-flower extract, paracetamol and distilled water. The results showed that leaf-flower extract at a dose of 300 mg /200 g BW decreased the rectal temperature greater than with doses of 100 and 200 mg /200 g BW for 180 minutes of measurement. Conclusion: Leaf-flower extract has antipyretic effect in Wistar rats.Keywords: antipyretic, phyllanthus niruri, DPT-HB, wistar ratAbstrak: Penelitian ini bertujuan untuk menguji efek antipiretik ekstrak meniran (Phyllanthus niruri L.) pada tikus Wistar yang diinduksi vaksin DPT-HB. Metode: Hewan uji yang digunakan adalah 15 ekor tikus Wistar yang dibagi ke dalam 5 kelompok, masing-masing kelompok terdiri dari 3 ekor tikus. Kelompok kontrol negatif diberikan aquades, kelompok kontrol positif diberikan parasetamol, dan 3 kelompok eksperimental diberikan ekstrak tanaman meniran masing-masing dengan dosis 100mg, 200mg dan 300 mg/200 grBB tikus. Induksi demam pada hewan uji menggunakan vaksin DPT-HB 0,2ml secara intramuskular. Pengukuran suhu rektal dilakukan sebelum dan sesudah pemberian vaksin dan setelah pemberian bahan uji yaitu pada menit ke-30, 60, 90, 120, 150 dan 180. Hasil penelitian memperlihatkan pemberian ekstrak meniran dengan dosis 300 mg/200 grBB menunjukkan penurunan suhu rektal lebih besar dibanding dengan dosis 100 dan 200 mg/200 grBB selama 180 menit pengukuran. Simpulan: Ekstrak meniran memiliki efek antipiretik pada tikus Wistar.Kata kunci: antipiretik, phyllanthus niruri, DPT-HB, tikus wistar

An In Situ Study of the Influence of Staining Beverages on Color Alteration of Bleached Teeth

2016 ◽  
Vol 41 (6) ◽  
pp. 627-633 ◽  
Author(s):  
AL Briso ◽  
TC Fagundes ◽  
MO Gallinari ◽  
J Moreira ◽  
LCAG de Almeida ◽  
...  
Keyword(s):  
Color Change ◽  
Grape Juice ◽  
Study Groups ◽  
Positive Control ◽  
Negative Control ◽  
Distilled Water ◽  
Dental Bleaching ◽  
Tooth Color ◽  
Mean Values ◽  
Bleaching Treatment

SUMMARY The aim of this study was to evaluate overall color change in bovine tooth fragments submitted to dental bleaching treatment performed simultaneously with the ingestion of beverages containing dyes. For this purpose, tooth fragments assembled into intraoral devices were submitted to at-home dental bleaching using 10% carbamide peroxide (CP) for 14 days and to immersion in staining beverages for 10 minutes daily. The specimens were divided into the following study groups according to bleaching treatment and staining substance (n=12): G I (negative control): no bleaching + distilled water; G II (positive control): bleaching + distilled water; G III: bleaching + coffee; and G IV: bleaching + grape juice. Twelve volunteers used the device continually, except during meals, oral hygiene, dental bleaching, and pigment challenge. Color readings were performed using a spectrophotometer both before the bleaching treatment and after each treatment week. The results were submitted to the normality test. The data obtained were submitted to analysis of variance and the Tukey or Kruskal-Wallis and Dunn tests (α=0.05). All bleached groups showed similar ΔE results at the end of treatment. Staining beverages generated negative ΔL mean values, and the lowest result was obtained in the treatment with coffee after 14 days. The Δa values in the groups that received treatment with staining beverages were higher when compared to the control groups. Dental bleaching associated with the consumption of staining substances may not affect overall tooth color change by the end of the treatment, although the consumption of staining substances did influence the different color dimensions.

Effects of Fluoride on the Mouse Sperm Morphology Test

1987 ◽  
Vol 66 (9) ◽  
pp. 1509-1511 ◽  
Author(s):  
Y. Li ◽  
A.J. Dunipace ◽  
G.K. Stookey
Keyword(s):  
Sperm Morphology ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Distilled Water ◽  
Genotoxic Effects ◽  
Mouse Sperm ◽  
Abnormal Sperm ◽  
Group I ◽  
Cervical Dislocation

There has been little information and much confusion regarding the genotoxic effects of fluoride. The purpose of this study was to examine the spermatogenic influence of sodium fluoride (NaF) on the germ cells by means of the mouse sperm morphology test. Male mice of genotype B6C3F1 were obtained at about eight weeks of age and maintained on a low-fluoride diet (<0.2 ppm F) and distilled water ad libitum throughout the experiment. At approximately 13 weeks of age, the animals were randomly assigned to eight groups. Group I was intubated with the Maximum Tolerable Dosage (MTD) of NaF (70 mg/kg). Groups II through VI received NaF by stomach intubation at doses of 35, 20, 10, 1, and 0.1 mg/kg, respectively. Group VII served as a negative control and was intubated with distilled water. The positive control, Group VIII, was exposed to a known mutagen, cyclophosphamide (20 mglkg, i.p). The animals were treated daily for five days, and killed by cervical dislocation 35 days after the first exposure to chemicals. Slides of sperm from the cauda epididymides were prepared and blindly scored for morphological abnormalities. Weight of the testes was recorded, and the femurs were saved for fluoride (F) analysis. Analysis of bone F demonstrated the effective absorption of fluoride following intubation. The counts of abnormal sperm and the weights of the testes for mice exposed to NaF doses up to the MTD were not significantly different from those of the negative control. The results of this study showed that NaF did not have adverse effects on mouse sperm morphology.

scholarly journals Antibacterial Activity of Cold Powder Preparation of (Ethanol Extract) Starfruit Leaf (Averrhoa bilimbi Linn.) Against Propionibacterium acnes

2020 ◽  
Vol 6 (2) ◽  
pp. 120-128
Author(s):  
Helen Anjelina Simanjuntak ◽  
Kasta Gurning ◽  
Verawati Br. Sinaga
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plant ◽  
Research Method ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Distilled Water ◽  
Powder Preparation

Acne is a skin disease that occurs due to chronic trade that is triggered by the bacterium Propionibacterium acnes. Starfruit leaf (Averrhoa bilimbi Linn.) as a traditional medicinal plant which has antibacterial activity. The purpose of this study was to determine the antibacterial activity from ethanol extract in the preparations cold powder of starfruit leaf (Averrhoa bilimbi Linn.) againts Propionibacterium acnes. The research method was carried out experimentally, and testing for antibacterial activity was carried out using agar diffusion methods. Cold powder formulation such as F1 (5%), F2 (10%), F3 (15%), F4 (20%), positive control with clindamycin, negative control with distilled water. The results showed that the F3 (15%) and F4 (20%) formulations were more effective in inhibiting the growth of Propionibacterium acnes bacteria with inhibition zone diameters of 13.66 mm and 15.16 mm in the strong category

scholarly journals Effect of Long Bean (Vignaunguiculata) on Blood Glucose Level of Mice (Mus musculus) Induced by Alloxan

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Intan Fitri Aprila ◽  
M. Nur Salim ◽  
Razali Daud ◽  
T. Armansyah ◽  
Nuzul Asmilia ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Mus Musculus ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Distilled Water ◽  
Decrease Blood Glucose

The aim of this research was to find out the effect of long bean (Vigna unguiculata) on level of blood glucose on mice (Mus musculus) induced by alloxan. Twelve mice with the weigh of 25-40 grams and clinically healthy were used in this research. All mice were randomly divided into 4 treatment groups, 3 mice each. K0 was negative control group, only given aquadest. K1, K2, and K3 groups were induced by alloxan 0.5 ml. K1 group was positive control. K2 group was fed with 100 grams long bean mixed with 50 ml distilled water. K3 group was fed with 100 grams long bean mixed with 100 ml distilled water. Long bean was fed orally 0.5 ml every morning and evening for 7 consecutive days. On day eight the examination of blood glucose level was performed. The average level of blood glucose were K0 (142.00±23.39), K1 (167.00±10.54), K2 (122.67±12.50), dan K3 (154.67±16.26) mg/dL. In conclusion, the administration of long bean for seven consecutive days does not decrease blood glucose level on mice induced by alloxan.Key words: blood glucose, alloxan, long bean

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