scholarly journals Molecular Identification of a Novel Iflavirus in Brown-Spotted Pitvipers (Protobothrops Mucrosquamatus)

2021 ◽  
Author(s):  
Zhige Tian ◽  
Ying Deng ◽  
Hongzhi Cao ◽  
Miaomiao Zheng ◽  
Ke Li ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Genomic Data ◽  
Amino Acid Sequences ◽  
Lygus Lineolaris ◽  
Structural Genes ◽  
High Identity ◽  
Vespa Velutina ◽  
Enveloped Viruses ◽  
Rdrp Domain

Abstract Background: Iflaviridae is a family of small non-enveloped viruses with monopartite, positive-stranded RNA genomes, which are identified in arthropod hosts, primarily infecting insect species. Herein, we firstly identify the sequence of an iflavirus (YB-PMP20) found in brown-spotted pitvipers in China. Results: The sequence of YB-PMP20 showed high identity to the sequences of Hubei picorna-like virus (HUPV) (99.2% in nt), Vespa velutina-associated iflavirus like virus (VVAIV) (58.6% in nt) and Lygus lineolaris virus (LyIV-1) (46.6% in nt) in nucleotides encoding polyproteins. It contained a single large ORF (304–9291 nt) encoding 2996 amino acids. The deduced amino acid sequences were compared with those of iflavirus. Helicase, protease and the RdRp domain were found to be located at the 3´ end, and structural genes (VP1, VP2 and VP3) were found to be located at the 5´ end. Phylogenetic analysis indicated that YB-PMP20 belongs to the iflavirus cluster, and is similar to HUPV, LyIV-1 and VVAIV. Conclusion: The present study described the genetic characterization of a PmIFV strain in brown-spotted pitvipers. Our genomic data extend knowledge of the diversity of viruses in snakes.

scholarly journals Prevalence and Genetic Characterization of Two Mitochondrial Gene Sequences of Strobilocercus Fasciolaris in the Livers of Brown Rats (Rattus norvegicus) in Heilongjiang Province in Northeastern China

2020 ◽  
Vol 10 ◽  
Author(s):  
Fengnian Zhao ◽  
Yun Zhou ◽  
Yanchen Wu ◽  
Kexin Zhou ◽  
Aiqin Liu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Intraspecific Variation ◽  
Rattus Norvegicus ◽  
Genetic Characterization ◽  
Northeastern China ◽  
Amino Acid Sequences ◽  
Morphological Observation ◽  
Heilongjiang Province ◽  
Gene Sequences

Rodents constitute the largest and most successful group of mammals worldwide. Brown rats (Rattus norvegicus) are one of the most common rodent species, and they serve as intermediate hosts of Hydatigera taeniaeformis. Although there have been a few studies reporting on the presence of the larval form of H. taeniaeformis (strobilocercus fasciolaris) in brown rats worldwide, little information is available on the genetic characterization of this parasite, with no molecular data from China. Therefore, from April 2014 to March 2016, this study was carried out to understand the prevalence and genetic characters of strobilocercus fasciolaris in brown rats captured in Heilongjiang Province in northeastern China. The livers of brown rats were collected and examined for the presence of cysts. Each cyst was identified based on morphological observation: the larvae with the naked eye and the scolexes under a microscope. The results were confirmed by polymerase chain reaction (PCR) and sequencing of the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 4 (nad4) genes. At the investigated sites, 11.8% (13/110) of the brown rats were infected with strobilocercus fasciolaris. Based on sequence analysis, there were 10 and six haplotypes regarding the cox1 and the nad4 loci, with 24 and 42 polymorphic sites, respectively (degree of intraspecific variation: 0.3%–4.4% and 0.6%–4.7%, respectively). Twelve nucleotide sequences (six of the 10 at the cox1 locus and all six at the nad4 locus) have not previously been described. Base differences in three of the six novel cox1 gene sequences and five of the six novel nad4 gene sequences caused amino acid changes. Phylogenetic analyses of the cox1 and nad4 gene sequences based on neighbor-joining and Bayesian inference trees indicated that all the strobilocercus fasciolaris isolates belonged to Hydatigera taeniaeformis sensu stricto (s.s.). This is the first report on the genetic characterization of strobilocercus fasciolaris in brown rats in China. The findings of novel cox1 and nad4 nucleotide and amino acid sequences may reflect the region-specific genetic characterization of the parasite. The data will be useful to explore the biological and epidemiological significance of the intraspecific variation within H. taeniaeformis s.s.

scholarly journals Biochemical and Genetic Characterization of the Two-Peptide Bacteriocin Enterocin 1071 Produced by Enterococcus faecalis FAIR-E 309

2002 ◽  
Vol 68 (5) ◽  
pp. 2550-2554 ◽  
Author(s):  
Charles M. A. P. Franz ◽  
Alexandra Grube ◽  
Anette Herrmann ◽  
Hikmate Abriouel ◽  
Joachim Stärke ◽  
...  
Keyword(s):  
Amino Acids ◽  
Sequence Analysis ◽  
Enterococcus Faecalis ◽  
Dna Sequence ◽  
Genetic Characterization ◽  
Peptide Sequence ◽  
Transporter Gene ◽  
Structural Genes ◽  
Heterologous Host

ABSTRACT The structural genes for the two-peptide bacteriocin enterocin 1071 (Ent1071) in Enterococcus faecalis FAIR-E 309 were cloned. DNA sequence analysis showed that the enterocin 1071A (Ent1071A) peptide of strain FAIR-E 309 differed by two amino acids from the Ent1071A reported for E. faecalis BFE 1071 (E. Balla, L. M. T. Dicks, M. Du Toit, M. J. van der Merwe, and W. H. Holzapfel, Appl. Environ. Microbiol. 66:1298-1304, 2000), while the Ent1071B gene encoded identical peptides in these strains. However, resequencing of ent1071A from E. faecalis BFE 1071 showed that the Ent1071A peptide sequence reported previously was incorrect in two amino acids. Also, ent1071B in E. faecalis FAIR-E 309 encoded a prepeptide that was three amino acids shorter than that previously reported for E. faecalis BFE 1071 Ent1071B. A presumptive immunity gene (eni1071) was located downstream of the bacteriocin structural genes. This gene was cloned into the heterologous host E. faecalis ATCC 19433 and was shown to confer immunity. A truncated ABC transporter gene was located upstream of the Ent1071 structural genes.

scholarly journals Genetic characterization of rabies virus isolated from bovines and equines between 2007 and 2008, in the States of São Paulo and Minas Gerais

2010 ◽  
Vol 43 (2) ◽  
pp. 116-120 ◽  
Author(s):  
Carla Isabel Macedo ◽  
Pedro Carnieli Junior ◽  
Willian de Oliveira Fahl ◽  
Jonas Yoshitaka de Oliveira Lima ◽  
Rafael de Novaes Oliveira ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Genetic Characterization ◽  
Active Regions ◽  
Minas Gerais ◽  
Sao Paulo ◽  
Amino Acid Sequences ◽  
Biologically Active ◽  
Economic Losses ◽  
São Paulo

INTRODUCTION: Rabies is an acute disease of the central nervous system and is responsible for the deaths of thousands of humans, wild animals and livestock, particularly cattle, as well as causing major economic losses. This study describes the genetic characterization of rabies virus variants that circulate in Desmodus rotundus populations and are transmitted to herbivores. METHODS: Fifty rabies virus isolates from bovines and equines in the States of São Paulo and Minas Gerais, Brazil, were genetically characterized and compared with sequences retrieved from GenBank. RESULTS: Two clusters (I and II) with mean nucleotide identities of 99.1 and 97.6% were found. The first of these contained nearly all the samples analyzed. Lineages from other Brazilian states grouped in cluster II. CONCLUSIONS: Analysis of the amino acid sequences of the N proteins revealed the existence of genetic markers that may indicate possible variations between geographic regions, although the biologically active regions are conserved within the species over space and time.

scholarly journals Identification and Genetic Characterization of a Novel Respirovirus in Alpine Chamois (Rupicapra rupicapra rupicapra)

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 704
Author(s):  
Camilla Luzzago ◽  
Erika Ebranati ◽  
Antonio Lavazza ◽  
Martina Besozzi ◽  
Gianguglielmo Zehender ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Phylogenetic Analyses ◽  
Amino Acid Sequences ◽  
Species Demarcation Criterion ◽  
Demarcation Criterion ◽  
Protein Amino Acid ◽  
Rupicapra Rupicapra ◽  
Separate Branch ◽  
Alpine Chamois

The Respirovirus genus, family Paramamixoviridae, includes respiratory viral pathogens. Here we report the identification and genetic characterization of a respirovirus in an Alpine chamois showing interstitial pneumonia associated with catarrhal bronchopneumonia. The full-genome characterization of this respirovirus, named ChamoisRV/IT2014, revealed low similarities to caprine respirovirus (77.1%), bovine respirovirus (74.5%) and human respirovirus (72.0%). The phylogenetic analyses based on the full-length genome sequence of the novel isolate and reference respirovirus strains showed that ChamoisRV/IT2014 clustered with caprine respirovirus but formed a separate branch. The phylogenetic tree topology of complete large protein amino acid sequences, representing the current species demarcation criterion for Respirovirus genus, showed a 0.05 branch length of ChamoisRV/IT2014 sequence between the nearest node and the tip of the branch, suggesting that this virus belongs to a novel species. This new isolate in a new host species raises several questions to be addressed on the epidemiological role of chamois and the risks of cross-transmission between wild ruminants and livestock.

scholarly journals Genetic analysis of African swine fever virus based on major genes encoding p72, p54 and p30

2021 ◽  
Vol 20 (03) ◽  
pp. 18-25
Author(s):  
Duy T. Do
Keyword(s):  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
Complex Structure ◽  
Clinical Signs ◽  
African Swine Fever ◽  
Amino Acid Sequences ◽  
Sequencing Analysis ◽  
Genes Encoding ◽  
Genotype Ii

African swine fever (ASF) is reported as a highly contagious hemorrhage lethal disease of domestic and wild swine. The causative agent of ASF is a large enveloped DNA virus with a complex structure. There are twenty-four ASFV genotypes described to date. However, in Vietnam, only genotypes II had been previously described. The genetic characterization of ASFV enhances the understanding of ASF epidemiology in terms of the extent, severity, source, and potential genetic variations among ASFV strains circulating in Southern Vietnam. Twenty ASFV strains were collected from pigs with ASFV-infected clinical signs from 10 provinces during 2019 - 2020. Partial B646L (p72) gene, complete E183L (p54), and CP204L (p30) genes were amplified, purified, and sequenced. Phylogenetic analysis confirmed the circulation of genotype II by both the partial B646L (p72) and full-length E183 (p54) gene sequencing. Analysis of the p72, p54, and p30 regions did not indicate any change in the nucleotide and amino acid sequences among these strains. The results of this study revealed that these ASFVs shared high homology to ASFV isolates detected in Northern Vietnam and China.

Histopathological and genetic characterization of colorectal mucinous carcinoma

2001 ◽  
Vol 120 (5) ◽  
pp. A166-A166
Author(s):  
S FUJII ◽  
T KUSAKA ◽  
T KAIHARA ◽  
Y UEDA ◽  
T CHIBA ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Mucinous Carcinoma

Genetic characterization of high hyperdiploidy in childhood acute lymphoblastic leukemia

2009 ◽  
Vol 221 (03) ◽  
Author(s):  
R Vagkopoulou ◽  
C Eckert ◽  
U Ungethüm ◽  
G Körner ◽  
M Stanulla ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Genetic Characterization ◽  
Lymphoblastic Leukemia ◽  
Childhood Acute Lymphoblastic Leukemia

Characterization of a cDNA for Rhesus Monkey Protein C Inhibitor – Evidence for N-Terminal Involvement in Heparin Stimulation

1995 ◽  
Vol 74 (04) ◽  
pp. 1079-1087 ◽  
Author(s):  
Klaus-P Radtke ◽  
José A Fernández ◽  
Bruno O Villoutreix ◽  
Judith S Greengard ◽  
John H Griffin
Keyword(s):  
Rhesus Monkey ◽  
Protein C ◽  
Activated Protein C ◽  
Pcr Amplification ◽  
Amino Acid Sequences ◽  
Heparin Binding ◽  
Reactive Center ◽  
Protein C Inhibitor ◽  
Polymerase Chain

SummarycDNAs for protein C inhibitor (PCI) were cloned from human and rhesus monkey 1 liver RNAs by reverse transcription and polymerase chain reaction (PCR) amplification. Sequencing showed that rhesus monkey and human PCI cDNAs were 93% identical. Predicted amino acid sequences differed at 26 of 387 residues. Pour of these differences (T352M, N359S, R362K, L3631) were in the reactive center loop that is important for inhibitory specificity, and two were in the N-terminal helix (M8T, E13K) that is implicated in glycosaminoglycan binding. PCI in human or rhesus monkey plasma showed comparable inhibitory activity towards human activated protein C in the presence of 10 U/ml heparin. However, maximal acceleration of the inhibition of activated protein C required 5-fold lower heparin concentration for rhesus monkey than for human plasma, consistent with the interpretation that the additional positive charge (E13K) in a putative-heparin binding region increased the affinity for heparin.

TBE in Sweden

2020 ◽  
Keyword(s):  
Genetic Characterization ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Second Phase ◽  
Serum Samples ◽  
Tick Borne Encephalitis ◽  
Specific Immunoglobulin ◽  
Tick Borne Encephalitis Virus ◽  
First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

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