scholarly journals Effects of Sevoflurane at Different Concentrations on ApoE in Hippocampus of Aged Rats With Hypercholesterolemia

2021 ◽  
Author(s):  
Yuhang Zhu ◽  
Li Luo ◽  
Lin Zhou ◽  
Zhenyu Wu ◽  
Yang Yang ◽  
...  
Keyword(s):  
Optical Density ◽  
Immunofluorescence Assay ◽  
Behavioral Changes ◽  
Aged Rats ◽  
Mixed Gas ◽  
High Concentration ◽  
Sevoflurane Group ◽  
Average Optical Density ◽  
Significant Difference ◽  
Hippocampal Ca3

Abstract Objective: To observe the behavioral changes of aged rats with hypercholesterolemia after inhalation of 1.0 MAC and 1.3MAC sevoflurane , the levels of hippocampal ApoE3, ApoE4 and Aβ1-42, as well as the changes of Aβ1-42 levels of optical density in hippocampal CA3 and CA1 regions, and to investigate the effects of different concentrations of sevoflurane on cognitive function in aged rats with hypercholesterolemia.Method: The 15-month-old male SD rats were fed a high-fat diet for 9 months. Rats successfully modeled (N=54) were randomly divided into three groups:control group (Con group, n=18), low concentration sevoflurane group ( Sev1.0 group, n=18), high concentration sevoflurane group (Sev1.3 group, n=18). Rats in the three groups inhaled aero mixed gas(1L/min O2+1L/min Air), 1.0MAC sevoflurane and 1.3MAC sevoflurane for 2h respectively.1 day, 30 days and 90 days after sevoflurane treatment were defined as T1, T30 and T90 experimental periods, respectively. In the three experiments, 6 rats were randomly selected from Con group, Sev1.0 group and Sev1.3 group to complete the behavior experiment in Morris water maze.Subsequently, the levels of ApoE3, ApoE4 and Aβ1-42 in the left hippocampus were detected by Western Blot.Expression of Aβ1-42 in the right hippocampal CA3 and CA1 regions of rats in each group was detected by frozen immunofluorescence assay.Result: 1. No behavioral changes were found in T1, T30 and T90 experiments.2. At T1 and T30, ApoE4 and Aβ1-42 in Sev1.3 group was significantly different from that in Con group. At T90, there was no difference in the levels of ApoE4 and Aβ1-42 between groups. ApoE3 expression was not statistically significant in the three experimental periods. 3. At T1 and T30, the average optical density of Aβ1-42 in CA3 and CA1 region, Sev1.3 group was significantly different from that in Con group. At T90, there was no significant difference between groups.Conclusion: After inhalation of 1.3MAC sevoflurane in aged SD hypercholesterolemia rats, the levels of ApoE4 and Aβ1-42 were increased in hippocampus at early and middle stage, and the average optical density of Aβ1-42 was increased in CA3 and CA1 area. The change trend of the two ones was consistent, but not enough to cause behavioral changes.

scholarly journals Rheumatologist perspective of the Brazilian consensus for detection of auto antibodies in HEp-2 CELLS

2021 ◽  
Vol 61 (1) ◽  
Author(s):  
Isadora Carvalho Medeiros Francescantonio ◽  
Leandro Augusto Rodrigues dos Santos ◽  
Paulo Luiz Carvalho Francescantonio ◽  
Luiz Eduardo Coelho Andrade ◽  
Wilson de Melo Cruvinel
Keyword(s):  
Clinical Practice ◽  
Immunofluorescence Assay ◽  
Chi Square ◽  
Dichotomous Data ◽  
Level Of Satisfaction ◽  
Significant Difference ◽  
Chi Square Test ◽  
Geographic Regions ◽  
Relevant Pattern ◽  
Structured Questionnaire

Abstract Objective To evaluate the perception of rheumatologists regarding the recommendations of the Brazilian Consensus for detection of Autoantibodies (BCA) on HEp-2 Cells by Indirect Immunofluorescence assay (IFA) and how BCA recommendations help in clinical practice. Methodology A structured questionnaire regarding the BCA recommendations for detection and interpretations of autoantibodies in HEp-2 cells was applied to randomly selected rheumatologists. The results were tabulated using the Microsoft® Excel program, expressed as a simple percentage and the dichotomous data were analyzed using the Chi-square test and the Epi Info® program. Results Four hundred fuorteen rheumatologists participated in the study: 70% of them considered their knowledge of the HEp-2 IFA test satisfactory or excellent, and 43% said they knew the BCA recommendations in general, without distinguishing the edition of the BCA to which they refer. The Revista Brasileira de Rheumatologia/Advances in Rheumatology was the means of dissemination most consulted by specialists (50%). According to the rheumatologists’ opinion, the most relevant pattern was the homogeneous nuclear (78%) and 65% stated they were satisfied with the BCA recommendations at a level of satisfaction greater than or equal to 80%. There was no significant difference in the perception of rheumatologists from the several Brazilian geographic regions. Conclusion Brazilian rheumatologists are aware of the BCA guidelines and most are satisfied with the content published, considering that the BCA recommendations assist positively in the clinical practice. Most rheumatologists recognize the patterns associated with rheumatic autoimmune diseases and have used BCA recommendations to interpret the results of the HEp-2 IFA test.

Comparative Study on Vit. E & Mefenamic acid vs Mefenamic acid alone on mean reduction in pain in Primary Dysmenorrhea

2021 ◽  
Vol 15 (12) ◽  
pp. 3195-3197
Author(s):  
Fariha Sadiqa ◽  
Mufakhara Fatimah ◽  
Abdul Mudabbir Rehan ◽  
Sidra Mushtaq ◽  
Asia Firdous ◽  
...  
Keyword(s):  
Vitamin E ◽  
Mefenamic Acid ◽  
Controlled Trial ◽  
Acid Group ◽  
P Value ◽  
Primary Dysmenorrhea ◽  
High Concentration ◽  
Randomized Controlled ◽  
Significant Difference ◽  
Menstrual Fluid

Background: Pelvic pain around the time of mensturation without any identifiable pathologic lesion present from menarche is called primary dysmenorrhea. The pain is believed to be related to prostaglandin (PG). Women with dysmenorrhoea have a relatively high concentration of PGF 2 alpha in menstrual fluid and suppression of PG synthesis has become the main treatment. Aim: To compare mean reduction in pain in patients presenting with primary dysmenorrhea given vitamin E & Mefenamic acid versus Mefenamic acid alone. Results: It was a randomized controlled trial which was conducted in Department of Obstetrics & Gynecology, THQ Raiwind Hospital, Lahore for 6 months duration w.e.f 01/02/2017 to 31/07/2017. In this study, 18(36%) in Vitamin-E group and 21(42%) in Mefenamic acid group were between 15-20 years while 32(64%) in Vitamin-E group and 29(58%) in Mefenamic acid group were between 21-25 years, mean±sd was calculated as 20.86±2.92 and 20.66±2.86 years respectively, mean dysmenorrheal pain at baseline was recorded as 50.06±10.27 in Vitamin-E group and 50.14±10.28 in Mefenamic acid group, p value < 0.754, showing that both groups are insignificant, mean dysmenorrheal pain after treatment was recorded as 20.50±10.04 in Vitamin-E group and 30.22±10.28 in Mefenamic acid group, p value was < 0.002 showing significant difference between the two group, comparison of mean reduction in dysmenorrheal pain after treatment was recorded as 20.56±0.91 in Vitamin-E group and 10.92±0.75 in Mefenamic acid group, p value was < 0.000, showing significant difference. Conclusion: We concluded that there is a significant mean reduction in dysmenorrhic pain in patients given Mefenamic Acid + Vitamen E as compared to patients given Mefenamic Acid alone. Keywords: Dysmenorrhic pain, Mefenamic Acid + Vitamen E, mean reduction in dysmenorrhic pain

22. Immune Response to Stenotrophomonas maltophilia in Cystic Fibrosis Patients

2018 ◽  
Author(s):  
Jillian Wettlaufer
Keyword(s):  
Cystic Fibrosis ◽  
Immune Response ◽  
Clinical Outcomes ◽  
Optical Density ◽  
Stenotrophomonas Maltophilia ◽  
Negative Control ◽  
Chronic Patients ◽  
Significant Difference ◽  
Antibody Levels ◽  
Sera Samples

Background: Stenotrophomonas maltophilia is one of the most common multidrug-resistant organisms isolated from the cystic fibrosis (CF) respiratory tract but it is unknown how it influences the long term clinical outcomes of CF patients.   Objective/Hypothesis: To characterize the immune response to S. maltophilia and its association with clinical outcomes in CF patients over time.  Methods: This was a longitudinal study from 2007-2014 of CF patients followed at The Hospital for Sick Children and St. Michael’s Hospital. All patients were classified as: 1) those with chronic S. maltophilia: ³2 positive cultures/year, 2) intermittent S. maltophilia: 1 positive sputum culture/year, and 3) no S. maltophilia cultures/year with and without chronic P. aeruginosa. IgG/IgA/IgM serologic responses were measured in serial sera samples by ELISA using whole cell S. maltophilia antigen. Results were calculated as the ratio of the average serum sample optical density to the average optical density of the negative control wells. Antibody levels for each patient were compared longitudinally to their rate of decline in FEV1 % predicted, body mass index, and rate of hospitalization.   Results: S. maltophilia antibody levels were measured in 350 sera samples from 113 CF patients. Median baseline antibody levels were 1.56 (range 0.996-5.15) in chronic patients, 1.09 (range 0.907-3.79) in intermittent patients, and 1.12 (range 0.737-4.86) in patients with no S. maltophilia.  Conclusions: Preliminary data suggests antibody levels to be significantly higher in patients with chronic S. maltophilia, and no significant difference between intermittent S. maltophilia and no S. maltophilia.

scholarly journals Impact of Biochar on Douglas-Fir Tussock Moth (Orgyia pseudotsugata Lepidoptera: Erebidae) Larvae Reared on Synthetic Die

Insects ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1065
Author(s):  
Stacey Rice-Marshall ◽  
Stephen P. Cook ◽  
John Randall
Keyword(s):  
Weight Gain ◽  
Larval Mortality ◽  
Douglas Fir ◽  
High Concentration ◽  
Larval Weight ◽  
Synthetic Diet ◽  
Tussock Moth ◽  
Orgyia Pseudotsugata ◽  
Compensatory Feeding ◽  
Significant Difference

The use of biochar as a soil amendment in forest ecosystems can be beneficial in the restoration of degraded soils. Forest insects such as the Douglas-fir tussock moth, Orgyia pseudotsugata (McDonnough) (Lepidoptera: Erebidae), may be exposed to biochar when the material is applied. Two experiments were conducted using biochar either (1) applied to the surface of the diet at three rates (0, 5, and 10 mg) or (2) incorporated into synthetic diet at four rates (0, 10, 20, and 40% volume/volume). The objective of both experiments was to determine if biochar on the surface or incorporated into a synthetic diet affected development and survival of O. pseudotsugata larvae. In both experiments, there was a significant decrease in estimated time to larval mortality in all biochar treatments compared to untreated controls. In the surface-applied biochar experiment, there was a significant difference in larval weight gain at day 12 between the control and 10 mg biochar treatments. In the experiment with biochar incorporated into the diet, mean larval weight at day 12 was highest in the low (10%) biochar treatment compared to all other treatments, although weight gain was only significantly different between the low- and high-concentration (40%) biochar treatments. Our results suggest that larvae, feeding on a low amount of biochar in the synthetic diet, may respond by engaging in compensatory feeding behavior. Fewer surviving larvae in the biochar treatment groups may contribute to the lack of significance found in the comparison of weight gain at day 24 in each experiment.

scholarly journals A Novel Engineered Single-Chain Antibody Fragment for Targeting Pediatric Philadelphia Chromosome-like Acute Lymphoblastic Leukemia

Blood ◽  
2020 ◽  
Vol 136 (Supplement 1) ◽  
pp. 36-36
Author(s):  
Sara M.A. Mohamed ◽  
Keith Sia ◽  
Karl-Heinz Friedrich ◽  
Andreas Wohlmann ◽  
Savvas Savvides ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Conflicts Of Interest ◽  
Lymphoblastic Leukemia ◽  
Statistical Significance ◽  
Immunofluorescence Assay ◽  
Laser Scanning Microscopy ◽  
Indirect Immunofluorescence Assay ◽  
Single Chain ◽  
Genetic Characteristics ◽  
Significant Difference

Introduction: Philadelphia-like acute lymphoblastic leukaemia (Ph-like ALL) is a high-risk ALL subtype characterized by an inferior survival rate and chemotherapeutic drug resistance (Tasian et al, Blood 130: 2064-2072, 2017). Around 50% of Ph-like ALL cases harbour gene rearrangements leading to the overexpression of cytokine receptor-like factor 2 (CRLF2) (Loh et al, Blood 121: 485-488, 2013). CRLF2 (also known as thymic stromal lymphopoietin receptor, TSLPR) heterodimerizes with the interleukin-7 receptor alpha (IL-7Rα) subunit to form the functional TSLPR. Upon TSLP binding, CRLF2 activates the JAK/STAT signalling pathway, leading to enhanced proliferation and survival of leukemia cells resulting in poor outcomes in patients (Harvey et al, Blood 115: 5312-5321, 2010). The extracellular overexpression of CRLF2 and association with poor prognosis suggest the translational value of designing precision-based therapeutics targeting CRLF2 in Ph-like ALL. Although conventional immunotherapy using full-sized antibodies is a promising treatment strategy that can improve treatment efficiency and minimize off-target toxicity, their clinical translation is challenging due to the high production cost and large size affecting targeting efficiency (Holliger et al, Nat Biotech 23: 1126-1136, 2005). Herein, we validated a novel single-chain variable fragment against CRLF2 (CRLF2-ScFv) for targeting Ph-like ALL cells. Methods: A CRLF2-rearranged Ph-like ALL cell line (MHH-CALL-4) was lentivirally transduced with a CRLF2-targeting shRNA driven by an inducible promoter, enabling the inducible knockdown of CRLF2. CRLF2 expression in MHH-CALL-4 cells after shRNA induction (KD-CALL-4) was evaluated using fluorescence-activated cell sorting (FACS). The cellular association of the CRLF2-ScFv was determined in MHH-CALL-4 and KD-CALL-4 at 4° and 37°C using an indirect immunofluorescence assay. Confocal laser scanning microscopy was used to visualize and compare the cellular association of CRLF2-ScFv in MHH-CALL-4 and KD-CALL-4. The cellular association of CRLF2-ScFv was also investigated ex vivo using a small panel of Ph-like and non-Ph-like ALL patient-derived xenografts (PDXs), representing similar immunophenotype and genetic characteristics to their original disease subtypes (Liem et al, Blood 103: 3905-3914, 2004), and peripheral blood mononuclear cells (PBMCs) to investigate the non-selective association. CRLF2 expression in MHH-CALL-4 and Ph-like ALL PDX cells was quantified using indirect immunofluorescence assay. The downstream impact of CRLF2-ScFv on STAT5 phosphorylation (pSTAT5) was determined by FACS either with or without TSLP stimulation. The statistical significance was tested using Unpaired unequal variances t-test or one-way ANOVA followed by multiple comparisons test. Statistical significance was considered when P ≤ 0.05. All experiments were performed in triplicate. Results: KD-CALL-4 showed a 75% reduction in CRLF2 expression compared with MHH-CALL-4 cells (P = 0.0009). CRLF2-ScFv exhibited a 94% higher association with MHH-CALL-4 compared with KD-CALL-4 cells at 37°C (P = 0.0013). The association of CRLF2-ScFv with MHH-CALL-4 cells was reduced by 75% at the non-proliferating state of cells at 4°C compared to 37°C (P = 0.006). Orthogonally viewed confocal microscopy images showed 82% higher intracellular uptake of CRLF2-ScFv in MHH-CALL-4 compared to KD-CALL-4 cells (P = 0.0003). CRLF2-ScFv showed &gt;80% higher association with a Ph-like PDX sample compared with a control CRLF2low PDX and PBMCs (P &lt; 0.001). Of note, a Ph-like ALL PDX exhibited only one-third of the association with CRLF2-ScFv compared with MHH-CALL-4 cells (P = 0.04), corresponding to the significant difference in CRLF2 surface expression (P = 0.01). CRLF2-ScFv significantly reduced pSTAT5 expression in MHH-CALL-4 cells (P = 0.05) and prevented TSLP-induced STAT5 phosphorylation (P = 0.01), suggesting competition with the TSLP binding site. Conclusion: CRLF2-ScFv is a selective targeting moiety for CRLF2 with a significant internalization potential and receptor antagonistic effect, highlighting the therapeutic implications for targeting Ph-like ALL. Keywords: CRLF2, ScFv, STAT5 phosphorylation, Patient-Derived Xenografts. Disclosures No relevant conflicts of interest to declare.

Studies of Exchange Coupling in Fe/Cu/Fe(001) “Loose Spin” Structures

1995 ◽  
Vol 384 ◽  
Author(s):  
M. Kowalewski ◽  
B. Heinrich ◽  
K. Totland ◽  
J.F. Cochran ◽  
S. Govorkov ◽  
...  
Keyword(s):  
Exchange Coupling ◽  
Interlayer Coupling ◽  
Antiferromagnetic Coupling ◽  
Concentration Limit ◽  
Interlayer Exchange Coupling ◽  
High Concentration ◽  
Significant Difference ◽  
Valence Electrons ◽  
Interlayer Exchange

ABSTRACTThe interlayer exchange coupling has been studied in two trilayer structures:(a) 5.7Fe/5Cu/1FecCu1-c/5Cu/10Fe(001), where c=0.0, 0.1, 0.2, 0.45 0.60(b) 5.7Fe/5Cu/1CrcCu1-c/5Cu/10Fe(001), where c=0.1, 0.45, 0.8 and 1.0.The intention of these studies was to identify the role of Fe and Cr atoms in the alloyed FecCu1-c and CrcCu1-c layers on the direct interlayer coupling which is facilitated by the Cu valence electrons. FMR, BLS and MOKE studies were used to determine the interlayer exchange coupling. Mossbauer spectroscopy was used to identify the magnetic state of the Fe atoms in the alloyed layer. The results showed that the presence of foreign atoms inside the Cu spacer significantly decreased the bilinear antiferromagnetic coupling between the Fe layers. In the low concentration limit the Fe and Cr atoms behaved in a similar manner. A significant difference was found in the high concentration limit where the Fe atoms start to be partially magnetically ordered.

scholarly journals Corticotrophin releasing factor receptor 1 antagonists prevent chronic stress-induced behavioral changes and synapse loss in aged rats

2018 ◽  
Vol 90 ◽  
pp. 92-101 ◽  
Author(s):  
Hongxin Dong ◽  
Jack M. Keegan ◽  
Ellie Hong ◽  
Christopher Gallardo ◽  
Janitza Montalvo-Ortiz ◽  
...  
Keyword(s):  
Chronic Stress ◽  
Behavioral Changes ◽  
Aged Rats ◽  
Synapse Loss ◽  
Corticotrophin Releasing Factor

Photodosimeter Badge System

PEDIATRICS ◽  
1985 ◽  
Vol 75 (2) ◽  
pp. 437-441
Author(s):  
Peter C. Scheidt ◽  
Phyllis M. Sternthal ◽  
Robert Anderson ◽  
Robert Studholme ◽  
Dolores A. Bryla ◽  
...  
Keyword(s):  
Child Health ◽  
Human Development ◽  
Optical Density ◽  
Action Spectrum ◽  
Radiant Energy ◽  
National Academy Of Sciences ◽  
High Concentration ◽  
Film Badge ◽  
The Difference ◽  
Academy Of Sciences

The Committee on Phototherapy in the Newborn of the National Academy of Sciences has recommended10 development of a compact system to continuously measure radiant energy exposure of infants treated with phototherapy. A small bilirubinimpregnated photodosimeter film badge was developed by the Beckman Instrument Company for this purpose and was used during the National Institute of Child Health and Human Development (NICHD) phototherapy study. This report summarizes the results of data obtained with this measurement device and briefly describes the performance of this system. METHODS The film badge dosimeter system consists of a 5.2x2.6-cm plastic badge composed of an imbedded disk of bilirubin cast in a polymeric substrate and sandwiched between two layers of clear plastic. Before exposure to light, the badge exhibits a high optical density at 460 nm due to a relatively high concentration of bilirubin. The optical density of the badge at 400 to 500 nm decreases as a function of the total dose of light within the action spectrum for the photodecomposition of bilirubin to which the badge has been exposed. Initial optical density at 460 nm is measured by a densitometer designed and dedicated specifically for this purpose. After exposure, the optical density is again measured by insertion into the densitometer; the total dosage, which is the time-integrated irradiance received by the badge during the photodecomposition process, is derived from the difference in optical density between the two readings. (A description of the calibration of the badge is available on request from Biometry Branch, Epidemiology and Biometry Research Program, National Institute of Child Health and Human Development, Bethesda, Maryland.)

scholarly journals FK960, a novel cognitive enhancer, increases synapses in the hippocampal CA3 region of aged rats

2000 ◽  
Vol 82 ◽  
pp. 238
Author(s):  
Akira Moriguchi ◽  
Kazuo Nakano ◽  
Kouichi Sano ◽  
Kosei Noda ◽  
Nobuya Matsuoka ◽  
...  
Keyword(s):  
Aged Rats ◽  
Cognitive Enhancer ◽  
Hippocampal Ca3 ◽  
Ca3 Region

scholarly journals Assessment of Vascular Endothelial Growth Factor in Fresh versus Frozen Platelet Rich Plasma

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Nada Hosny ◽  
Fikry Goubran ◽  
Basma BadrEldin Hasan ◽  
Noha Kamel
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Calcium Gluconate ◽  
Platelet Rich Plasma ◽  
Vascular Endothelial ◽  
High Concentration ◽  
Significant Difference ◽  
Growth Factor Release ◽  
Endothelial Growth Factor ◽  
Vegf Release

Platelet rich plasma (PRP) is hemoconcentration with platelets concentration above baseline values and high concentration of many growth factors. The aim of this study was to assess freezing effect on vascular endothelial growth factor (VEGF) release from PRP using two different activation methods to simplify its use in different clinical applications. PRP was prepared using two-centrifugation steps method from 12 qualified blood donors. VEGF concentrations were measured in fresh PRP and after freezing/thawing for one and three weeks with two methods of activation using (i) calcium gluconate and (ii) calcium gluconate and thrombin. Platelets count was significantly increased compared to baseline whole blood values in all fresh and frozen PRP samples (p value was <0.05). No significant difference was found between VEGF concentrations after activating fresh and frozen-thawed PRP samples for one and three weeks by calcium alone or calcium with thrombin, and also no significant difference was found when freezing period was extended from one to three weeks. Our results showed that platelets count does not correlate with variable levels of VEGF. PRP could be prepared once and preserved frozen for at least three weeks for the next treatment sessions and activation with thrombin addition to calcium will not augment the growth factor release.

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