scholarly journals Acupoint Nanocomposite Hydrogel for Simulation of Acupuncture and Targeted Delivery of Triptolide Against Rheumatoid Arthritis

2021 ◽  
Author(s):  
Shujing Ren ◽  
Heng Liu ◽  
Xitong Wang ◽  
Jiquan Bi ◽  
Shengfeng Lu ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Drug Delivery ◽  
Targeted Delivery ◽  
Pain Threshold ◽  
Modern Medicine ◽  
Inflammatory Factors ◽  
Nanocomposite Hydrogel ◽  
Analgesic Effects ◽  
Anti Inflammatory ◽  
Targeting Ability

Abstract BackgroundAttenuating the inflammatory response and relieving pain are two therapeutical goals for rheumatoid arthritis (RA). Anti-inflammatory and analgesic drugs are often associated with many adverse effects due to nonspecific distribution. New drug delivery systems with effective targeting ability and other complementary strategies are on urgent need to be explored. To achieve this goal, an acupoint drug delivery system that can simulate acupuncture in relieving pain and targeted deliver anti-inflammatory drugs is constructed, which can co-deliver 2-chloro-N (6)-cyclopentyl adenosine (CCPA) and triptolide (TP). ResultsWe have successfully demonstrated that the nanocomposite hydrogel composed of TP-Human serum album nanoparticles (HSA NPs) and CCPA could effectively treat the RA. We found that this combination therapy can enhance analgesic effects while the mechanical pain threshold was 5.2 times compared with model group, and the thermal pain threshold was 1.4 times. Acupoint nanocomposite hydrogel could not only improve the accumulation of the designed nanomedicine in arthritic paws (13.5% higher than those in non-acupoint at 48h), but also cooperate with nanomedicine to exert synergetic improvement of inflammation and reduction of systemic toxicity. Furthermore, it can regulate inflammatory factors and restore the balance of Th17/Treg cell which provide a novel effective treatment strategy for RA.ConclusionThis novel therapeutic approach-acupoint nanocomposite hydrogel, builds a bridge between acupuncture and drugs which sheds light on the combination of traditional and modern medicine.

scholarly journals Acupoint nanocomposite hydrogel for simulation of acupuncture and targeted delivery of triptolide against rheumatoid arthritis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Shujing Ren ◽  
Heng Liu ◽  
Xitong Wang ◽  
Jiquan Bi ◽  
Shengfeng Lu ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Drug Delivery ◽  
Targeted Delivery ◽  
Bone Erosion ◽  
Treg Cells ◽  
Modern Medicine ◽  
Inflammatory Factors ◽  
Nanocomposite Hydrogel ◽  
Analgesic Effects ◽  
Anti Inflammatory

Abstract Background Attenuating inflammatory response and relieving pain are two therapeutic therapeutical goals for rheumatoid arthritis (RA). Anti-inflammatory and analgesic drugs are often associated with many adverse effects due to nonspecific distribution. New drug delivery systems with practical targeting ability and other complementary strategies urgently need to be explored. To achieve this goal, an acupoint drug delivery system that can target deliver anti-inflammatory drugs and simulate acupuncture in relieving pain was constructed, which can co-deliver triptolide (TP) and 2-chloro-N (6)-cyclopentyl adenosine (CCPA). Results We have successfully demonstrated that acupoint nanocomposite hydrogel composed of TP-Human serum album nanoparticles (TP@HSA NPs) and CCPA could effectively treat RA. The result shows that CCPA-Gel can enhance analgesic effects specifically at the acupoint, while the mechanical and thermal pain threshold was 4.9 and 1.6 times compared with non-acupoint, respectively, and the nanocomposite gel further enhanced. Otherwise, the combination of acupoint and nanocomposite hydrogel exerted synergetic improvement of inflammation, bone erosion, and reduction of systemic toxicity. Furthermore, it could regulate inflammatory factors and restore the balance of Th17/Treg cells, which provided a novel and effective treatment strategy for RA. Interestingly, acupoint administration could improve the accumulation of the designed nanomedicine in arthritic paws (13.5% higher than those in non-acupoint at 48 h), which may explain the better therapeutic efficiency and low toxicity. Conclusion This novel therapeutic approach-acupoint nanocomposite hydrogel, builds a bridge between acupuncture and drugs which sheds light on the combination of traditional and modern medicine. Graphical Abstract

Therapeutic Properties of PDMS Nanoparticles: A Promising New Drug Delivery Vehicle Against Inflammatory Conditions

2021 ◽  
Vol 24 ◽  
Author(s):  
Aiswarya Anilkumar Ajitha ◽  
Sri SivaKumar ◽  
Gayathri Viswanathan ◽  
Sabulal Baby ◽  
Prabath Gopalakrishnan Biju
Keyword(s):  
Drug Delivery ◽  
Targeted Delivery ◽  
Release Kinetics ◽  
Drug Loading ◽  
Systemic Circulation ◽  
Biological Evaluation ◽  
Raw 264.7 Cells ◽  
Morphological Examination ◽  
Inflammatory Conditions ◽  
Anti Inflammatory

Background: Over the last few decades, there has been a stupendous change in the area of drug delivery using particulate delivery systems, with increasing focus on nanoparticles in recent times. Nanoparticles helps to improve and alter the pharmacodynamic properties and pharmacokinetics of various types of drug molecules. These features help to protect the drug entity in the systemic circulation, access of the drug to the chosen sites, and to deliver the drug in a controlled and sustained rate at the site of action. Objective: Nanoparticle based targeted delivery of anti-inflammatory drugs/signal modulatory agents to the cytoplasm or nuclei of the targeted cell can significantly enhance the precision and efficacy of intended therapeutic activity. To this end, we report ligand free, enhanced intra-nuclear delivery model of anti-inflammatory therapeutics via PDMS nanoparticles. Method: PDMS nanoparticles were prepared by sacrificial silica template-based approach and details of their characterization for suitability as a nanoparticle-based delivery material is detailed herein. Results: Biological evaluation for compatibility was carried out and the results showed that the PDMS nanoparticle has no toxicity on RAW 264.7 cells in the concentration range of 10, 20, 40, 60, 80, 100 and 120 μg/mL in culture. Biocompatibility and absence of toxicity was determined by morphological examination and cell viability assays. Drug loading and release kinetics were carried out with the anti-inflammatory drug Diclofenac. Conclusion: In this paper we clearly demonstrate the various aspects of nanoparticle articulation, characterization, effect of their characteristics and their applications as a non-toxic drug delivery molecule for its potential applications in therapeutic delivery of drugs for sustained release.

scholarly journals Repairing and Analgesic Effects of Umbilical Cord Mesenchymal Stem Cell Transplantation in Mice with Spinal Cord Injury

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Ling-ling Wu ◽  
Xiao-ming Pan ◽  
Hao-hao Chen ◽  
Xiao-yan Fu ◽  
Jinzhan Jiang ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Treatment Group ◽  
Pain Threshold ◽  
Inflammatory Factors ◽  
Histological Evaluation ◽  
Spinal Cord Tissue ◽  
Model Group ◽  
Analgesic Effects ◽  
Cord Injury ◽  
Over Time

Transplantation of human umbilical cord mesenchymal stem cells (hUC-MSCs) into spinal cord injury (SCI) may alleviate neuropathic pain and promote functional recovery. The underlying mechanism likely involves activation of glial cells and regulation of inflammatory factors but requires further validation. SCI was induced in 16 ICR mice using an SCI compression model, followed by injection of lentiviral vector-mediated green fluorescent protein- (GFP-) labeled hUC-MSCs 1 week later. Behavioral tests, histological evaluation, and inflammatory factor detection were performed in the treatment (SCI+hUC-MSCs) and model (SCI) groups. Histological evaluation revealed GFP expression in the spinal cord tissue of the treatment group, implying that the injected MSCs successfully migrated to the SCI. The Basso, Beattie, and Bresnahan (BBB) scores showed that motor function gradually recovered over time in both groups, but recovery speed was significantly higher in the treatment group than in the model group. The pain threshold in mice decreased after SCI but gradually increased over time owing to the self-repair function of the body. The corresponding pain threshold of the treatment group was significantly higher than that of the model group, indicating the therapeutic and analgesic effects of hUC-MSCs. Expression of IL-6 and TNF-α in the spinal cord tissue of the treated group decreased, whereas glial cell line-derived neurotrophic factor (GDNF) expression along with ED1 expression increased compared with those in the model group, suggesting that SCI activated ED1 inflammatory macrophages/microglia, which were subsequently reduced by hUC-MSC transplantation. hUC-MSCs are speculated to enhance the repair of the injured spinal cord tissue and exert an analgesic effect by reducing the secretion of inflammatory factors IL-6 and TNF-α and upregulating the expression of GDNF.

scholarly journals Localized and targeted delivery of NSAIDs for treatment of inflammation: A review

2018 ◽  
Vol 244 (6) ◽  
pp. 433-444 ◽  
Author(s):  
Rebecca M Haley ◽  
Horst A von Recum
Keyword(s):  
Drug Delivery ◽  
Side Effects ◽  
Targeted Delivery ◽  
Local Drug Delivery ◽  
Future Research ◽  
Systemic Delivery ◽  
Number Of Patients ◽  
Long Term Treatment ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

Inflammatory processes are increasingly being identified at the core of many different disease states (e.g. heart disease, cancer, diabetes). As such, anti-inflammatory strategies available through drug delivery have undergone renewed interest. Due to the systemic side effects of steroidal drugs, non-steroidal anti-inflammatory drugs are often preferred for long-term treatment of inflammation in a variety of applications. While non-steroidal anti-inflammatory drugs are generally safe, there are some serious side effects that can be associated with their usage, particularly when given systemically or orally. Due to the high number of patients taking non-steroidal anti-inflammatory drugs, the reduction or elimination of these side effects, such as is possible through local drug delivery, could have a very powerful effect on patient quality of life. This review comments on a sampling of existing methods for localized or targeted delivery of non-steroidal anti-inflammatory drugs, with the goal of helping future research groups to focus on bettering methods shown to be effective and filling the gaps of knowledge in this field. Additionally, commentary is made on the field as a whole, and the standardization issues that arise from its expansiveness and diversity. Impact statement This work provides an overview of research currently being done exploring potential drug delivery device strategies for NSAIDs as an alternative to systemic delivery. Commentary on this field is made in an attempt to aid future experimental design, enabling researchers to determine the drugs and delivery vehicles which are most advantageous for them to pursue, as well as suggestions to standardize the reporting of such future research.

scholarly journals Lung-targeted Macrophage Biomimetic Nanocarriers Based on Platycodon Grandiflorum Polysaccharides for Calming Cytokine Storm in Pneumonia

2021 ◽  
Author(s):  
Yi Li ◽  
Chunjing Guo ◽  
Qiang Chen ◽  
Yanguo Su ◽  
Huimin Guo ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Targeted Delivery ◽  
Severe Pneumonia ◽  
Scientific Basis ◽  
Cytokine Storm ◽  
Platycodon Grandiflorum ◽  
Life Threatening ◽  
Targeting Ability ◽  
Delivery Efficiency

Abstract Background Pneumonia is a life-threatening respiratory disease without effective treatment due to uncontrolled inflammation of the lung tissue. Suppression of cytokine storms may be one of the keys to saving the lives of patients with severe pneumonia. Given the fragile delivery efficiency of drugs in vivo, novel delivery platforms to address these issues are necessary. Results Here, we developed a biomimetic nanocarrier (MNPs) with macrophage membranes coated ROS-responsive Platycodon grandiflorum polysaccharides nanoparticles (PNPs) for targeted delivery of curcumin (MNPs@Cur) to inflamed lungs and treat inflammation by calming cytokine storms. In the study, we could clearly find that MNPs@Cur significantly attenuated inflammation and cytokine storm syndrome in acute lung injury (ALI) mice by neutralizing multiple proinfammatory cytokines. Interestingly, we found that the PNPs also had potent pulmonary targeting compared to other polysaccharide carriers, which probably means that PNPs have inherited the natural targeting ability in the medicinal guide theory of Traditional Chinese Medicine (TCM). Conclusion The results demonstrated that the developed drug delivery system may serve as an effective and safe nanoplatform for the treatment of pneumonia, as well as provide experimental scientific basis for the medicinal guide theory of TCM and its clinical application.

scholarly journals Facile Construction of a Solely-DNA-Based System for Targeted Delivery of Nucleic Acids

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1967
Author(s):  
Ziwen Dai ◽  
Juan Li ◽  
Yongfang Lin ◽  
Zhigang Wang ◽  
Yang Huang
Keyword(s):  
Drug Delivery ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Drug Delivery System ◽  
Delivery System ◽  
Targeted Delivery ◽  
Dna Aptamers ◽  
Dna Nanostructures ◽  
Dna Strands ◽  
Targeting Ability

We designed a functional drug delivery system based solely on DNA. The whole system was built with only four DNA strands. Cyclization of DNA strands excluded the formation of byproducts. DNA aptamers were equipped to endow triangular DNA nanostructures with targeting ability. The homogeneity of materials enabled not only facile construction but also convenient loading of nucleic acid-based drugs with much ease.

Bioerodible Polymeric Nanoparticles for Targeted Delivery of Proteic Drugs

2006 ◽  
Vol 6 (9) ◽  
pp. 3040-3047 ◽  
Author(s):  
Elisabetta E. Chiellini ◽  
Federica Chiellini ◽  
Roberto Solaro
Keyword(s):  
Drug Delivery ◽  
Targeted Delivery ◽  
Polymeric Nanoparticles ◽  
Hybrid Nanoparticles ◽  
Polymeric Systems ◽  
Nanoparticle Surface ◽  
Biological Tests ◽  
Targeting Ability ◽  
Average Size ◽  
Targeted Release

Significant efforts are being devoted to develop nanotechnology for drug delivery, mainly because of the distinct advantages offered by nanometer-size polymeric systems. Moreover, targeted drug delivery can be obtained by polymer conjugation to biospecific ligands. The present investigation was aimed mainly at determining the targeting ability of hybrid nanoparticles based on synthetic polymer/protein hybrid matrices. These nanoparticles were designed for liver targeted release of proteic drugs with antiviral activity, such as α-interferon. Human serum albumin and the monoesters of alternating copolymers of maleic anhydride/alkyl vinyl ethers of oligo(ethylene glycol) were selected as proteic and synthetic components, respectively. Digalactosyl diacyl glycerol, a natural glycolipid selectively recognized by the asialofetuin receptor present on liver hepatocytes was used as active targeting agent. Nanoparticles of 100–300 nm average size were obtained by controlled coprecipitation method. Investigation of nanoparticle surface properties by spectroscopic analysis and by biological tests indicated that the synthesized nanoparticles do expose on their surface targeting moieties that selectively interact with liver hepatocytes receptors.

scholarly journals Green Synthesis of Gold and Iron Nanoparticles for Targeted Delivery: An In Vitro and In Vivo Study

2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
Beenish Khanzada ◽  
Nosheen Akthar ◽  
Muhammad Zeeshan Bhatti ◽  
Hammad Ismail ◽  
Mohammed Alqarni ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Medicinal Plants ◽  
Targeted Delivery ◽  
Iron Nanoparticles ◽  
Drug Loading ◽  
Research Work ◽  
Ficus Microcarpa ◽  
Anti Inflammatory

Nanotechnology has vast applications in almost all fields of science and technology. The use of medicinal plants for the synthesis of metallic nanoparticles has gained much attention nowadays. In the current research work, six medicinal plants were used for the synthesis of gold nanoparticles (AuNPs) and iron nanoparticles (FeNPs). The synthesized nanoparticles were characterized by different techniques including UV-visible spectrophotometry, scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR). Furthermore, the activities of green synthesized nanoparticles were screened in vitro using, for example, antibacterial, antioxidant, cytotoxic, and DNA protection assays. Both FeNPs and AuNPs had spherical shapes with an average size less than 50 nm and were found to have good antimicrobial and nontoxic effects. Furthermore, FeNPs from Ficus microcarpa demonstrated high drug loading efficiency (65%) as compared to an anti-inflammatory drug (diclofenac potassium, DFP). We also evaluated the drug delivery potential, as well as anti-inflammatory and anticoagulant properties, of nanoparticles in vivo. Interestingly, AuNPs of Syzygium cumini exhibited strong anti-inflammatory potential as compared to DFP and diclofenac-loaded FeNPs of Ficus microcarpa. The results suggest potential pharmacological applications of biogenic synthesized AuNPs and FeNPs which can be explored further. The study revealed that the green synthesized AuNPs and FeNPs provide a promising approach for the synthesis of drug-loaded nanoparticles and consequently in the field of targeted drug delivery.

ANALGESIC AND ANTI-INFLAMMATORY ACTIVITY OF METHANOLIC EXTRACT FROM JATROPHA CURCAS (EUPHORBIACEAE) LEAVES ON EXPERIMENTAL ANIMALS

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (08) ◽  
pp. 32-38
Author(s):  
P Chandra ◽  
◽  
N Sachan ◽  
R Yadav ◽  
K. Kishore ◽  
...  
Keyword(s):  
Jatropha Curcas ◽  
Pain Threshold ◽  
Methanolic Extract ◽  
Paw Edema ◽  
Traditional Use ◽  
Analgesic Effects ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Analgesic Activities ◽  
Rat Paw

The present study evaluates the analgesic and anti-inflammatory activities of the methanolic extract from Jatropha curcas (Euphorbiaceae) leaves to provide experimental evidence for its traditional use. Investigations on the analgesic effects and anti-inflammatory activities of J. curcas were carried out by utilizing the different animal models. It has been shown that the methanolic extract from Jatropha curcas leaves significantly increased pain threshold and reduced writhing response as well as inhibited the increase in vascular permeability. Also, it significantly decreased the carrageenan-induced rat paw edema. The results show that the methanolic extract from Jatropha curcas leaves have both central and peripheral analgesic activities and as anti-inflammatory effects, supporting the traditional application of this herb in treating various diseases associated with inflammation and pain.

scholarly journals Update on Nanoparticle-Based Drug Delivery System for Anti-inflammatory Treatment

2021 ◽  
Vol 9 ◽  
Author(s):  
Huailan Wang ◽  
Yunxiang Zhou ◽  
Qunan Sun ◽  
Chenghao Zhou ◽  
Shiyao Hu ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Drug Delivery ◽  
Inflammatory Bowel Disease ◽  
Drug Delivery Systems ◽  
Inflammatory Diseases ◽  
Future Perspective ◽  
Efficient Delivery ◽  
Inflammatory Bowel ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

Nanobiotechnology plays an important role in drug delivery, and various kinds of nanoparticles have demonstrated new properties, which may provide opportunities in clinical treatment. Nanoparticle-mediated drug delivery systems have been used in anti-inflammatory therapies. Diseases, such as inflammatory bowel disease, rheumatoid arthritis, and osteoarthritis have been widely impacted by the pathogenesis of inflammation. Efficient delivery of anti-inflammatory drugs can reduce medical dosage and improve therapeutic effect. In this review, we discuss nanoparticles with potential anti-inflammatory activity, and we present a future perspective regarding the application of nanomedicine in inflammatory diseases.

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