Noninvasive technology of photostimulation of lymphatic clearance of red blood cells from the mouse brain after intraventricular hemorrhage.

2021 ◽  
Author(s):  
Dongyu Li ◽  
Shaojun Liu ◽  
Tingting Yu ◽  
Zhang Liu ◽  
Silin Sun ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Intraventricular Hemorrhage ◽  
Lymphatic Vessels ◽  
Hematoma Expansion ◽  
Non Invasive ◽  
Clearing Functions ◽  
Fatal Form ◽  
First Time ◽  
New Strategies

Abstract Intraventricular hemorrhage (IVH) is the most fatal form of brain injury in adults and infants. However, therapy of IVH is very limited, and new strategies are needed to reduce hematoma expansion. For the first time, in our experiments on mice with IVH, we clearly demonstrate that transcranial low-level infra-red photostimulation (PS) accelerates the red blood cells (RBCs) evacuation from the ventricles that is associated with reducing the mortality in 1.57 times, improving emotional status and recovery of intracranial pressure. We revealed the mechanisms of PS-modulation of lymphatic contractility and tone of the lymphatic vessels underlying the lymphatic clearing functions. Our findings strongly suggest that PS-mediated stimulation of lymphatic clearance of RBCs can be a novel non-invasive, bedside, readily applicable, and commercially viable technology for the treatment of IVH.

scholarly journals Photostimulation of lymphatic clearance of red blood cells from the mouse brain after intraventricular hemorrhage

2020 ◽  
Author(s):  
Dong-Yu Li ◽  
Shao-Jun Liu ◽  
Ting-Ting Yu ◽  
Zhang Liu ◽  
Si-Lin Sun ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Intraventricular Hemorrhage ◽  
Therapeutic Effects ◽  
Invasive Therapy ◽  
Non Invasive ◽  
Clearing Functions ◽  
Promising Therapeutic Target ◽  
Fatal Form ◽  
Stimulation Of

AbstractIntraventricular hemorrhage (IVH) is the most fatal form of brain injury, yet a therapy directed at ameliorating intraventricular clot is very limited. There is accumulating evidence that an augmentation of the meningeal lymphatic (MLVs) functions might be a promising therapeutic target for IVH. In particular, the photostimulation (PS) of MLVs could be promising for non-invasive therapy of IVH via PS of clearance of red blood cells (RBCs) from the brain via MLVs. Indeed, we uncover that PS has therapeutic effects on IVH in mice reducing the mortality, improving the emotional status, accelerating the RBCs evacuation from the ventricles and increasing the ICP recovery. Our findings strongly suggest that the PS-mediated stimulation of drainage and clearing functions of MLVs can be a novel bedside, readily applicable and commercially viable technologies for treatment of IVH. These pilot results open new horizons in a non-invasive therapy of IVH via PS stimulation of regenerative lymphatic mechanisms.

SCANNING ELECTRON MICROSCOPE: POSSIBILITIES FOR STUDYING KIDNEY DISEASES

2020 ◽  
pp. 176-182
Author(s):  
Mamaeva S.N. ◽  
Vinokurov R.R. ◽  
Munkhalova Ya.A. ◽  
Dyakonova D.P. ◽  
Platonova V.A. ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Filtration Rate ◽  
Kidney Diseases ◽  
First Time ◽  
Scanning Electron

Currently, due to the intensive development of high-tech science-intensive medical and research devices, more and more attention is paid to the development of diagnostics of rare and difficult to diagnose diseases. It is known that among numerous nephropathies, hematuria may be the only symptom of kidney and urinary tract diseases, which complicates their diagnosis and treatment. In order to develop new approaches for the diagnosis of nephropathies, the authors have been studying the morphology of red blood cells in the blood and urine of children and adults using a scanning electron microscope for several years. The paper presents the results of studies of children with various kidney diseases, including IgA-nephropathy, and chronic glomerulonephritis. Scanning electron microscopy was used for the first time to detect nanoparticles on the surface of red blood cells, the size of which is comparable to the size of viruses, which became the basis for one of the authors ' assumptions, namely, the possible transport of certain types of viruses by red blood cells. Thus, some kidney diseases could be considered virus-associated. This paper presents for the first time the results of determining the glomerular filtration rate of both kidneys separately in the study of separate kidney function and of the study of urine smears obtained during catheterization of the ureters in patients with hydronephrosis of one of the kidneys by scanning electron microscopy. As in previous studies, nanoparticles were found on the surface of red blood cells, which leads to the conclusion about the possible viral nature of the disease of the considered patient. In addition, smear images obtained using a microscope showed a significant difference in the elements of the right and left kidneys urine, which did not contradict the data on the study of glomerular filtration rate. According to the authors, the capabilities of the scanning electron microscope can be applied in fundamental research of kidney diseases at the cellular and molecular levels, forming new ideas about their origin, as well as on the basis of which new methods of non-invasive diagnostics can be built.

EFFECT OF STENOSIS SEVERITY ON SHEAR-INDUCED DIFFUSION OF RED BLOOD CELLS IN CORONARY ARTERIES

2019 ◽  
Vol 19 (05) ◽  
pp. 1950034 ◽  
Author(s):  
ABDULRAJAK BURADI ◽  
SUMANT MORAB ◽  
ARUN MAHALINGAM
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Mixture Theory ◽  
Theory Approach ◽  
Newtonian Viscosity ◽  
Maximum Fluctuation ◽  
Stenosis Severity ◽  
Flux Model ◽  
First Time ◽  
Arterial Endothelial Cells

In large blood vessels, migration of red blood cells (RBCs) affects the concentration of platelets and the transport of oxygen to the arterial endothelial cells. In this work, we investigate the locations where hydrodynamic diffusion of RBCs occurs and the effects of stenosis severity on shear-induced diffusion (SID) of RBCs, concentration distribution and wall shear stress (WSS). For the first time, multiphase mixture theory approach with Phillips shear-induced diffusive flux model coupled with Quemada non-Newtonian viscosity model has been applied to numerically simulate the RBCs macroscopic behavior in four different degrees of stenosis (DOS) geometries, viz., 30%, 50%, 70% and 85%. Considering SID of RBCs, the calculated average WSS increased by 77.70% which emphasises the importance of SID in predicting hemodynamic parameters. At the stenosis throat, it was observed that 85% DOS model had the lowest concentration of RBCs near the wall and highest concentration at the center. For the stenosis models with 70% and 85% DOS, the RBC lumen wall concentration at the distal section of stenosis becomes inhomogeneous with the maximum fluctuation of 1.568%. Finally, the wall regions with low WSS and low RBC concentrations correlate well with the atherosclerosis sites observed clinically.

scholarly journals Monitoring the side effects with DSC caused by cyclophosphamide treatment

2019 ◽  
Vol 142 (2) ◽  
pp. 765-770
Author(s):  
Péter Farkas ◽  
Franciska Könczöl ◽  
Dénes Lőrinczy
Keyword(s):  
Side Effects ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Peripheral Nerves ◽  
Chemotherapeutic Agents ◽  
Cyclophosphamide Treatment ◽  
Micro Dsc ◽  
Induced Changes ◽  
First Time ◽  
Dose Dependent

AbstractPolyneuropathy is defined as a simultaneous malfunction of several peripheral nerves, which could be a side effect of a cancer therapy (using cyclophosphamide) as well. In the daily use, it is very important to know the kinetics and metabolism of anticancer drugs because this way we can estimate their undesirable consequences to avoid the unwanted complications. Nowadays, the application of DSC in diagnosis of different diseases using blood compounds is increasing; therefore, the purpose of recent study was to introduce at the first time the DSC in the detection of cyclophosphamide-induced changes in plasma and red blood cells in case of an experimental animal model. Guinea pigs (Cavia porcellus, n = 60, in 12 different groups) underwent to cyclophosphamide treatment administrated intraperitoneally with the dose comparable to the human dosage. At the end of treatments, the animals were euthanized, and the experimental samples (plasma end red blood cells) were analyzed by a SETARAM Micro DSC-II calorimeter. The denaturation temperatures and the calorimetric enthalpies were calculated from the heat flow curves. Our results exhibited a dose-dependent difference between thermal parameters of untreated and treated samples, demonstrating that DSC is applicable in this field too. After deconvolution of DSC scans, the changes could be assigned to the attacked compounds. Recently published papers call the attention for this technique more frequently in the medical diagnosis because this way from small amount of sample very quickly and economically possible to detect and predict the expected and unwanted side effects of chemotherapeutic agents.

scholarly journals Evaluation of an Integrated Smart Sensor System for Real-Time Characterization and Digitalization of Postoperative Abdominal Drain Output: A Pilot Study

2021 ◽  
pp. 155335062110314
Author(s):  
Mario V. Roser ◽  
Alexander H. R. Frank ◽  
Lea Henrichs ◽  
Christian Heiliger ◽  
Dorian Andrade ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Real Time ◽  
Blood Cells ◽  
Modern Medicine ◽  
Sensor System ◽  
Smart Sensor ◽  
Routine Laboratory ◽  
Laboratory Measurements ◽  
Abdominal Drain ◽  
First Time

Background: For centuries, surgeons have relied on surgical drains during postoperative care. Despite all advances in modern medicine and the area of digitalization, as of today, most if not all assessment of abdominal secretions excreted via surgical drains are carried out manually. We here introduce a novel integrated Smart Sensor System ( Smart Drain) that allows for real-time characterization and digitalization of postoperative abdominal drain output at the patient’s bedside. Methods: A prototype of the Smart Drain was developed using a sophisticated spectrometer for assessment of drain output. The prototype measures 10 × 6 × 6 cm and therefore easily fits at the bedside. At the time of measurement with our Smart Drain, the drain output was additionally sent off to be analyzed in our routine laboratory for typical markers of interest in abdominal surgery such as bilirubin, lipase, amylase, triglycerides, urea, protein, and red blood cells. A total of 45 samples from 19 patients were included. Results: The measurements generated were found to correlate with conventional laboratory measurements for bilirubin (r = .658, P = .000), lipase (r = .490, P = .002), amylase (r = .571, P = .000), triglycerides (r = .803, P = .000), urea (r = .326, P = .033), protein (r = .387, P = .012), and red blood cells (r = .904, P = .000). Conclusions: To our best knowledge, for the first time we describe a device using a sophisticated spectrometer that allows for real-time characterization and digitalization of postoperative abdominal drain output at the patient’s bedside.

Non invasive method to measure red blood cells transit time through the microcirculation of the brain

2016 ◽  
Vol 8 (3-4) ◽  
pp. 461-466
Author(s):  
T. Herrmann ◽  
E. Gremillet ◽  
J. Juge ◽  
A. Champailler ◽  
P. Rusch ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Transit Time ◽  
Blood Cells ◽  
Non Invasive ◽  
Invasive Method ◽  
The Brain

scholarly journals Non-invasive evaluation of a liver mass in a patient post splenectomy

2018 ◽  
Vol 64 (1) ◽  
pp. 35-39
Author(s):  
Bella Smolen ◽  
Johad Khoury ◽  
Yaacov Baruch ◽  
Tarek Saadi
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Correct Diagnosis ◽  
Rare Condition ◽  
Liver Lesions ◽  
Extensive Investigation ◽  
Liver Mass ◽  
Case Presentation ◽  
Non Invasive ◽  
Invasive Evaluation

Introduction Numerous conditions may cause liver lesions, solitary or multiple, benign or malignant. It can be crucial to establish the correct diagnosis. Splenosis is a rare condition that may result from the spillage of cells from the splenic pulp following abdominal trauma, accidental lesions to the spleen during operation or elective splenectomy. These splenic ‘implants’, which are often multiple, can be located anywhere in the peritoneal cavity, although they are most often found in the left upper quadrant of the abdomen. They may be confused with neoplasms or endometriosis, and may rarely be the cause of small bowel obstruction. Case presentation A 35-year-old man presented with a hepatic mass, and malignancy was suspected. After extensive investigation, it was diagnosed as splenosis using Tc-99m-labelled heat-denaturated red blood cells scintigraphy, without the need for liver biopsy. We consider this the most effective method for diagnosing splenosis. Conclusion When splenosis is suspected, Tc-99m-labelled heat-denaturated red blood cells scintigraphy can be used to confirm the diagnosis, and may avoid invasive investigation.

Sign in / Sign up

Export Citation Format

Share Document