scholarly journals Evaluation of an Integrated Smart Sensor System for Real-Time Characterization and Digitalization of Postoperative Abdominal Drain Output: A Pilot Study

2021 ◽  
pp. 155335062110314
Author(s):  
Mario V. Roser ◽  
Alexander H. R. Frank ◽  
Lea Henrichs ◽  
Christian Heiliger ◽  
Dorian Andrade ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Real Time ◽  
Blood Cells ◽  
Modern Medicine ◽  
Sensor System ◽  
Smart Sensor ◽  
Routine Laboratory ◽  
Laboratory Measurements ◽  
Abdominal Drain ◽  
First Time

Background: For centuries, surgeons have relied on surgical drains during postoperative care. Despite all advances in modern medicine and the area of digitalization, as of today, most if not all assessment of abdominal secretions excreted via surgical drains are carried out manually. We here introduce a novel integrated Smart Sensor System ( Smart Drain) that allows for real-time characterization and digitalization of postoperative abdominal drain output at the patient’s bedside. Methods: A prototype of the Smart Drain was developed using a sophisticated spectrometer for assessment of drain output. The prototype measures 10 × 6 × 6 cm and therefore easily fits at the bedside. At the time of measurement with our Smart Drain, the drain output was additionally sent off to be analyzed in our routine laboratory for typical markers of interest in abdominal surgery such as bilirubin, lipase, amylase, triglycerides, urea, protein, and red blood cells. A total of 45 samples from 19 patients were included. Results: The measurements generated were found to correlate with conventional laboratory measurements for bilirubin (r = .658, P = .000), lipase (r = .490, P = .002), amylase (r = .571, P = .000), triglycerides (r = .803, P = .000), urea (r = .326, P = .033), protein (r = .387, P = .012), and red blood cells (r = .904, P = .000). Conclusions: To our best knowledge, for the first time we describe a device using a sophisticated spectrometer that allows for real-time characterization and digitalization of postoperative abdominal drain output at the patient’s bedside.

SCANNING ELECTRON MICROSCOPE: POSSIBILITIES FOR STUDYING KIDNEY DISEASES

2020 ◽  
pp. 176-182
Author(s):  
Mamaeva S.N. ◽  
Vinokurov R.R. ◽  
Munkhalova Ya.A. ◽  
Dyakonova D.P. ◽  
Platonova V.A. ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Filtration Rate ◽  
Kidney Diseases ◽  
First Time ◽  
Scanning Electron

Currently, due to the intensive development of high-tech science-intensive medical and research devices, more and more attention is paid to the development of diagnostics of rare and difficult to diagnose diseases. It is known that among numerous nephropathies, hematuria may be the only symptom of kidney and urinary tract diseases, which complicates their diagnosis and treatment. In order to develop new approaches for the diagnosis of nephropathies, the authors have been studying the morphology of red blood cells in the blood and urine of children and adults using a scanning electron microscope for several years. The paper presents the results of studies of children with various kidney diseases, including IgA-nephropathy, and chronic glomerulonephritis. Scanning electron microscopy was used for the first time to detect nanoparticles on the surface of red blood cells, the size of which is comparable to the size of viruses, which became the basis for one of the authors ' assumptions, namely, the possible transport of certain types of viruses by red blood cells. Thus, some kidney diseases could be considered virus-associated. This paper presents for the first time the results of determining the glomerular filtration rate of both kidneys separately in the study of separate kidney function and of the study of urine smears obtained during catheterization of the ureters in patients with hydronephrosis of one of the kidneys by scanning electron microscopy. As in previous studies, nanoparticles were found on the surface of red blood cells, which leads to the conclusion about the possible viral nature of the disease of the considered patient. In addition, smear images obtained using a microscope showed a significant difference in the elements of the right and left kidneys urine, which did not contradict the data on the study of glomerular filtration rate. According to the authors, the capabilities of the scanning electron microscope can be applied in fundamental research of kidney diseases at the cellular and molecular levels, forming new ideas about their origin, as well as on the basis of which new methods of non-invasive diagnostics can be built.

Real-Time Vision Using a Smart Sensor System

2007 ◽  
Author(s):  
Ahmed Nabil Belbachir ◽  
Martin Litzenberger ◽  
Christoph Posch ◽  
Peter Schon
Keyword(s):  
Real Time ◽  
Sensor System ◽  
Smart Sensor

scholarly journals An LC–MS Assay to Measure Superoxide Radicals and Hydrogen Peroxide in the Blood System

Metabolites ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 175 ◽  
Author(s):  
Ioannis Tsamesidis ◽  
Chinedu O. Egwu ◽  
Pierre Pério ◽  
Jean-Michel Augereau ◽  
Françoise Benoit-Vical ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Hydrogen Peroxide ◽  
Red Blood Cells ◽  
Blood Cells ◽  
High Resolution Mass Spectrometry ◽  
Modern Medicine ◽  
Reactive Species ◽  
Blood System ◽  
Pathological Conditions ◽  
Depth Study

Red blood cells are constantly exposed to reactive species under physiological or pathological conditions or during administration of xenobiotics. Regardless of the source, its accurate quantification is paramount in the area of theragnostics, which had been elusive up until now. Even if there are a lot of approaches to evaluate the oxidative stress, very sensitive methods are missing for the blood system. We therefore sought to apply a highly sensitive approach, by liquid chromatography coupled to mass spectrometry (UPLC–MS), for the quantification of reactive species such as superoxide radical and hydrogen peroxide using dihydroethidium (DHE) and coumarin boronic acid (CBA) probes respectively through the detection of 2-hydroxyethidium (2OH-E+) and 7-hydroxycoumarin (COH). The use of the high-resolution mass spectrometry associated to UPLC ensured a selective detection of superoxide and hydrogen peroxide in the blood system under diverse conditions such as oxidized red blood cells (RBCs), untreated and treated parasitized RBCs. Moreover, this technique allowed the determination of reactive species in human plasma. This protocol provides a huge opportunity for in-depth study of several pathological conditions vis-a-vis their treatment in modern medicine.

EFFECT OF STENOSIS SEVERITY ON SHEAR-INDUCED DIFFUSION OF RED BLOOD CELLS IN CORONARY ARTERIES

2019 ◽  
Vol 19 (05) ◽  
pp. 1950034 ◽  
Author(s):  
ABDULRAJAK BURADI ◽  
SUMANT MORAB ◽  
ARUN MAHALINGAM
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Mixture Theory ◽  
Theory Approach ◽  
Newtonian Viscosity ◽  
Maximum Fluctuation ◽  
Stenosis Severity ◽  
Flux Model ◽  
First Time ◽  
Arterial Endothelial Cells

In large blood vessels, migration of red blood cells (RBCs) affects the concentration of platelets and the transport of oxygen to the arterial endothelial cells. In this work, we investigate the locations where hydrodynamic diffusion of RBCs occurs and the effects of stenosis severity on shear-induced diffusion (SID) of RBCs, concentration distribution and wall shear stress (WSS). For the first time, multiphase mixture theory approach with Phillips shear-induced diffusive flux model coupled with Quemada non-Newtonian viscosity model has been applied to numerically simulate the RBCs macroscopic behavior in four different degrees of stenosis (DOS) geometries, viz., 30%, 50%, 70% and 85%. Considering SID of RBCs, the calculated average WSS increased by 77.70% which emphasises the importance of SID in predicting hemodynamic parameters. At the stenosis throat, it was observed that 85% DOS model had the lowest concentration of RBCs near the wall and highest concentration at the center. For the stenosis models with 70% and 85% DOS, the RBC lumen wall concentration at the distal section of stenosis becomes inhomogeneous with the maximum fluctuation of 1.568%. Finally, the wall regions with low WSS and low RBC concentrations correlate well with the atherosclerosis sites observed clinically.

scholarly journals Monitoring the side effects with DSC caused by cyclophosphamide treatment

2019 ◽  
Vol 142 (2) ◽  
pp. 765-770
Author(s):  
Péter Farkas ◽  
Franciska Könczöl ◽  
Dénes Lőrinczy
Keyword(s):  
Side Effects ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Peripheral Nerves ◽  
Chemotherapeutic Agents ◽  
Cyclophosphamide Treatment ◽  
Micro Dsc ◽  
Induced Changes ◽  
First Time ◽  
Dose Dependent

AbstractPolyneuropathy is defined as a simultaneous malfunction of several peripheral nerves, which could be a side effect of a cancer therapy (using cyclophosphamide) as well. In the daily use, it is very important to know the kinetics and metabolism of anticancer drugs because this way we can estimate their undesirable consequences to avoid the unwanted complications. Nowadays, the application of DSC in diagnosis of different diseases using blood compounds is increasing; therefore, the purpose of recent study was to introduce at the first time the DSC in the detection of cyclophosphamide-induced changes in plasma and red blood cells in case of an experimental animal model. Guinea pigs (Cavia porcellus, n = 60, in 12 different groups) underwent to cyclophosphamide treatment administrated intraperitoneally with the dose comparable to the human dosage. At the end of treatments, the animals were euthanized, and the experimental samples (plasma end red blood cells) were analyzed by a SETARAM Micro DSC-II calorimeter. The denaturation temperatures and the calorimetric enthalpies were calculated from the heat flow curves. Our results exhibited a dose-dependent difference between thermal parameters of untreated and treated samples, demonstrating that DSC is applicable in this field too. After deconvolution of DSC scans, the changes could be assigned to the attacked compounds. Recently published papers call the attention for this technique more frequently in the medical diagnosis because this way from small amount of sample very quickly and economically possible to detect and predict the expected and unwanted side effects of chemotherapeutic agents.

Binding Analysis of a Novel Peptide to Plasmodium falciparum Knob-Associated Histidine Rich Protein (KAHRP)

2007 ◽  
Vol 1 (1) ◽  
Author(s):  
J. Preston ◽  
L. Zeng ◽  
C. G. Takoudis ◽  
X. Li ◽  
A. Chishti
Keyword(s):  
Plasmodium Falciparum ◽  
Amino Acid ◽  
Red Blood Cells ◽  
Blood Vessels ◽  
Real Time ◽  
Optical Sensor ◽  
Blood Cells ◽  
Specific Binding ◽  
Binding Interaction ◽  
The Real

Knob-associated histidine rich protein (KAHRP) is secreted by Plasmodium falciparum in infected red blood cells. This protein is required for the production of surface protrusions called knobs, which have been shown to be crucial for the adherence of P. falciparum-infected erythrocytes (Pf-IRBC) to the endothelia of small blood vessels. KP-AP, a 10-amino acid (AA) peptide (FITRANDTSK), binds specifically with KAHRP in preliminary studies. KP-AP is expected to disrupt knob formation and prohibit adherence of Pf-IRBC to blood vessels and greatly reduce the pathogenicity of the parasite. This paper describes an investigation into the binding interaction between biotinylated KP-AP (Biotin-AP) and a segment of KAHRP. ELISA and the real-time bio-interaction optical sensor, BIAcore are the methods of detection. The specific binding was confirmed with ELISA and the KD value was estimated to be 1.2 μM. Binding was not detected with BIAcore, most likely due to the reduced flexibility of Biotin-AP while immobilized on the sensor chip.

scholarly journals Metabolite Profiling of Dihydroartemisinin in Blood of Plasmodium-Infected and Healthy Mice Using UPLC-Q-TOF-MSE

2021 ◽  
Vol 11 ◽  
Author(s):  
Yifan Zhao ◽  
Peng Sun ◽  
Yue Ma ◽  
Xiaoqiang Chang ◽  
Xingyu Chen ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Red Blood Cells ◽  
Metabolic Pathway ◽  
Blood Cells ◽  
Metabolite Profiling ◽  
First Line ◽  
First Time ◽  
Insight Into ◽  
Tof Ms

Dihydroartemisinin (DHA) and its’ derivatives have been employed as the most powerful first-line drugs for malarial treatment for several decades. The metabolism of DHA has not been studied clearly. Previous reports were focused on the pharmacokinetics procedure of DHA in healthy rats. The metabolites of DHA in red blood cells (RBC), especially in the RBC from Plasmodium-infected models, have rarely been studied. The Plasmodium species parasitize inside RBC, and these cells should be the final place where DHA performs its activity. In this study, the profile of DHA metabolites in biosample (blood, plasma, and RBC) of the infected and healthy mice was investigated with UPLC-Q-TOF-MS and UNIFI platform to gain insight into DHA metabolism. Results show that a total of 25 metabolites were successfully identified in infected (30 in healthy) blood, 27 in infected (27 in healthy) plasma, and 15 in infected (22 in healthy) RBC. Results show that hydroxylation, OH-dehydration, and glucuronidation reactions were important in the metabolic pathway in vivo. Significantly, DHA metabolites inside RBC were identified for the first time. 8-Hydroxy (8-OH) DHA, 4α-OH deoxy ART, and 6β-OH deoxy ART were identified in vivo for the first time.

Noninvasive technology of photostimulation of lymphatic clearance of red blood cells from the mouse brain after intraventricular hemorrhage.

2021 ◽  
Author(s):  
Dongyu Li ◽  
Shaojun Liu ◽  
Tingting Yu ◽  
Zhang Liu ◽  
Silin Sun ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Intraventricular Hemorrhage ◽  
Lymphatic Vessels ◽  
Hematoma Expansion ◽  
Non Invasive ◽  
Clearing Functions ◽  
Fatal Form ◽  
First Time ◽  
New Strategies

Abstract Intraventricular hemorrhage (IVH) is the most fatal form of brain injury in adults and infants. However, therapy of IVH is very limited, and new strategies are needed to reduce hematoma expansion. For the first time, in our experiments on mice with IVH, we clearly demonstrate that transcranial low-level infra-red photostimulation (PS) accelerates the red blood cells (RBCs) evacuation from the ventricles that is associated with reducing the mortality in 1.57 times, improving emotional status and recovery of intracranial pressure. We revealed the mechanisms of PS-modulation of lymphatic contractility and tone of the lymphatic vessels underlying the lymphatic clearing functions. Our findings strongly suggest that PS-mediated stimulation of lymphatic clearance of RBCs can be a novel non-invasive, bedside, readily applicable, and commercially viable technology for the treatment of IVH.

Sign in / Sign up

Export Citation Format

Share Document