scholarly journals A New OXA-66-harboring Acinetobacter Baumannii Sub-Clonal Complex 195 is Spreading Widely in Guangzhou, China, A Multicenter Study

2020 ◽  
Author(s):  
Yujun Li ◽  
Xiaomei Huang ◽  
Yuyao Wang ◽  
Chuzhi Pan ◽  
Zexun Mo ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Antimicrobial Agents ◽  
Clonal Complex ◽  
Genetic Relationships ◽  
Health Care Facilities ◽  
Hospital Environment ◽  
Teaching Hospitals ◽  
Care Facilities ◽  
Clonal Spread ◽  
Sequence Types

Abstract Objectives The clonal spread of Acinetobacter baumannii (A. baumannii) is an emerging problem. We analyzed the molecular epidemiology of A. baumannii isolated from 5 teaching hospitals in Guangzhou, China. Methods 138 A. baumannii isolates were collected. Multilocus sequence typing (MLST) was used to assess the genetic relationships among the isolates. The blaOXA-51-like gene was amplified and then sequenced. Results Most of the isolates (55.8%, 77/138) were obtained from intensive care units (ICUs). The respiratory system was the most common site where A. baumannii was found (72.5%, 100/138). A. baumannii remained susceptible to polymyxin and tigecycline, but the susceptibility to other antimicrobial agents was below 30%. We used MLST to group the A. baumannii isolates into 8 existing sequence types (STs) and 17 new STs. With the predicted founder ST195 (accounted for 35.5% of all isolates, 49/138), Clonal complex (CC) 195 was the most prevalent and widely spread STs in the hospital environment. All ST195 isolates harbored OXA-66 according to the blaOXA-51-like gene sequencing. But each hospital had its unique epidemiological feature. Conclusions OXA-66 gene harboring CC195 was the most epidemic STs in Guangzhou, China. Health care facilities should develop their own management strategy.

scholarly journals Molecular Survey of the Dissemination of TwoblaKPC-Harboring IncFIA Plasmids in New Jersey and New York Hospitals

2014 ◽  
Vol 58 (4) ◽  
pp. 2289-2294 ◽  
Author(s):  
Liang Chen ◽  
Kalyan D. Chavda ◽  
Roberto G. Melano ◽  
Tao Hong ◽  
Albert D. Rojtman ◽  
...  
Keyword(s):  
Health Care ◽  
New York ◽  
New Jersey ◽  
Plasmid Transfer ◽  
Health Care Facilities ◽  
Conjugative Plasmid ◽  
Content Type ◽  
Care Facilities ◽  
Clonal Spread ◽  
Sequence Types

ABSTRACTKlebsiella pneumoniaecarbapenemase (KPC)-producingK. pneumoniaestrains have spread worldwide and become a major threat in health care facilities. Transmission ofblaKPC, the plasmid-borne KPC gene, can be mediated by clonal spread and horizontal transfer. Here, we report the complete nucleotide sequences of two novelblaKPC-3-harboring IncFIA plasmids, pBK30661 and pBK30683. pBK30661 is 74 kb in length, with a mosaic plasmid structure; it exhibits homologies to several other plasmids but lacks the plasmid transfer operon (tra) and the origin of transfer (oriT) that are required for plasmid transfer. pBK30683 is a conjugative plasmid with a cointegrated plasmid structure, comprising a 72-kb element that highly resembles pBK30661 (>99.9% nucleotide identities) and an extra 68-kb element that harborstraandoriT. A PCR scheme was designed to detect the distribution ofblaKPC-harboring IncFIA (pBK30661-like and pBK30683-like) plasmids in a collection of clinicalEnterobacteriaceaeisolates from 10 hospitals in New Jersey and New York. KPC-harboring IncFIA plasmids were found in 20% of 491K. pneumoniaeisolates, and all carriedblaKPC-3. pBK30661-like plasmids were identified mainly in the epidemic sequence type 258 (ST258)K. pneumoniaeclone, while pBK30683-like plasmids were widely distributed in ST258 and otherK. pneumoniaesequence types and among non-K. pneumoniae Enterobacteriaceaespecies. This suggests that both clonal spread and horizontal plasmid transfer contributed to the dissemination ofblaKPC-harboring IncFIA plasmids in our area. Further studies are needed to understand the distribution of this plasmid group in other health care regions and to decipher the origins of pBK30661-like and pBK30683-like plasmids.

scholarly journals Perilaku Merokok Pengunjung dalam Mematuhi Peraturan Kawasan Tanpa Rokok (KTR) di Lingkungan RSUD S. K. Lerik Kota Kupang

2019 ◽  
Vol 1 (1) ◽  
pp. 8-14
Author(s):  
Imelda Fitryani Dam ◽  
Honey I. Ndoen ◽  
Indriati A. Tedjuhinga
Keyword(s):  
Health Care ◽  
General Hospital ◽  
Family Environment ◽  
Statistical Tests ◽  
Smoking Behavior ◽  
Health Care Facilities ◽  
Hospital Environment ◽  
Cross Sectional ◽  
Care Facilities ◽  
Level Of Knowledge

Non-smoking area is a room or area that is otherwise prohibited to activities of production, sales, advertising, promotion and use of cigarettes. Regional regulation Kupang City No. 3A year 2014 about non-smoking area explain that health care facilities, including hospitals is a place or non-smoking area. S. K. Lerik Municipality General Hopital is government owned health care facilities in Kupang and non-smoking area but still a lot of casual visitors who smoke in the hospital environment. The purpose of this research was to determine the relationship between the level of knowledge, education and family environment with the behavior of visitor to comply with regulation of non-smoking area at S. K. Lerik Kupang Municipality General Hospital 2016. The method of this research was analytical surveys using the cross-sectional design. The population in this research were all those at S. K. Lerik Kupang Municipality General Hospital environment with a total sample of 384 respondents. Statistical tests were used for data analysis in this research is Chi-Square with a degree of confidence α= 0.05. The results showed that there was correlation between the level of knowledge and education with smoking behavior of the visitor at S. K. Lerik Kupang Municipality General Hospital. There was no correlation between family environment with smoking behavior of the visitors at S. K. Lerik Kupang Municipality General Hospital.

scholarly journals Molecular Identification of Fusarium Species Recovered from Hospital Environment in Jos, Nigeria

2020 ◽  
pp. 303-308
Author(s):  
GE Kim ◽  
MO Okolo ◽  
UC Essien ◽  
UE Umeh ◽  
CC Iheukwumere
Keyword(s):  
Fusarium Species ◽  
Health Care Facilities ◽  
Control Measures ◽  
Hospital Environment ◽  
Chain Reaction ◽  
Opportunistic Fungi ◽  
Infection Control Measures ◽  
Hospital Environments ◽  
Care Facilities ◽  
Polymerase Chain

Fusariums pecies are opportunistic fungi that play an important role in nosocomial infection. The reservoir of Fusarium species in the hospital is not well understood in our environment. Therefore, the present study sought to identify the reservoir of Fusarium species in hospital environment. Three hundred and sixty (360) samples were collected from the environment of two tertiary health care facilities A and B. The sample consists of water (120), soil (120) and plants (120) which were sourced from hospital environments. Cultures of these samples were performed and polymerase chain reaction was used to confirm Fusarium species. The most predominant specie was Fusarium oxysporum Hospital A:(57.3%) and Hospital B:(64.4%). Most of the Fusarium isolates (76.7%) were recovered from soil samples, followed by water (45.0%) and the least were from plants (30.8%). In conclusion the present study has demonstrated that hospital environment is a reservoir for Fusarium species. However, identification of such reservoir would further enhance effective infection control measures.

scholarly journals REVIEWOF ACINETOBACTER BAUMANNII: TAXONOMY, EPIDEMIOLOGY, PATHOGENICITY, AND ANTIMICROBIAL RESISTANCE

2021 ◽  
Vol 03 (03) ◽  
pp. 18-24
Author(s):  
Khalida J. IBRAHEEM ◽  
Mohammed S. BAQER
Keyword(s):  
Acinetobacter Baumannii ◽  
The Elderly ◽  
Opportunistic Pathogen ◽  
Health Care Facilities ◽  
Multi Drug Resistance ◽  
Medical Field ◽  
Death Rates ◽  
Care Facilities ◽  
Different Types ◽  
Hospital Acquired

The genus Acinetobacter are a wide spread in nature, and there are at least 25 different types of them .In the medical field, Acinetobacter baumannii is one of the most important speciesof this genus . It is an opportunistic pathogen and have many virulence factors that make the bacteria capable of causing many hospital-acquired diseases which leads to nosocomial outbreak attendant with arise in death rates . This bacteria has the ability to be resistant to many antibiotics, and the emergence of high levels of multi-drug resistance A. baumannii has made it apriority health issue and is considered a serious threat to health care facilities, public health and the elderly . Which requires a tremendous effort to stop this escalation.

scholarly journals The Epidemiology and Control of Acinetobacter baumannii in Health Care Facilities

2006 ◽  
Vol 42 (5) ◽  
pp. 692-699 ◽  
Author(s):  
P. E. Fournier ◽  
H. Richet ◽  
R. A. Weinstein
Keyword(s):  
Health Care ◽  
Acinetobacter Baumannii ◽  
Health Care Facilities ◽  
Care Facilities ◽  
And Control

scholarly journals bla TEM, bla SHV and bla CTX-M-15 Extended Spectrum Beta-lactamase Produced by Acinetobacter baumanii, Enterobacter clocae and Proteus mirabilis from Pregnant Women in Three Secondary Health Care Facilities in South-south, Nigeria

2019 ◽  
pp. 1-9
Author(s):  
Florence Z. Uyanga ◽  
Emmanuel O. Ekundayo ◽  
Emmanuel O. Nwankwo
Keyword(s):  
Health Care ◽  
Pregnant Women ◽  
Acinetobacter Baumannii ◽  
Proteus Mirabilis ◽  
Bacterial Infections ◽  
Health Care Facilities ◽  
Bacterial Strains ◽  
Acinetobacter Baumanii ◽  
Care Facilities ◽  
Secondary Health Care

Background/Purpose: Based on fact there is high urinary tract infection and increasing treatment failure among pregnant women and this has led to increased mortality and morbidity among pregnant women, and increased stay in the hospital. This study was conducted to evaluate the prevalence of antimicrobial resistance and distribution of blaTEM, blaCTX-M-15 and blaSHV genes among A. baumannii, P. mirabilis and E. clocae strains isolated from urine samples from pregnant women attending antenatal at three secondary health care facilities south-south Nigeria. Methods: A. baumannii, P. mirabilis and E. clocae strains were isolated and identified using Microbact 24E. The disc diffusion and combined discs methods were used for testing antimicrobial susceptibility. The presence of ESBL was detected using Double Disk Synergy Test (DDST) and CHROMagar respectively. Plasmid extraction was carried out following the protocol of ZR Plasmid Miniprep-Classic extraction kit. Finally, the frequency of resistant genes including blaTEM, blaCTX-M-15 and blaSHV in selected 50 ESBL producing isolates was studied by PCR and using designed primers. Results: A total of 252 clinical isolates was collected from three secondary health care facilities in south-south, Nigeria. ESBLs were found in 231 (92%) isolates. blaCTX-M-15 was the commonest genotype (58.3%), followed by blaSHV (43.3%) and blaTEM (43.3%). Conclusion: ESBL positive strains of Enterobacter clocae, Acinetobacter baumannii and Proteus mirabilis are increasingly found in isolates from pregnant women. The widespread use of antibiotics has caused shifts in bacterial development to overcome the existing mechanisms of combating bacterial infections. These strains become resistant to frequently used antibiotics and they can pass the gene to other bacterial strains, the quick detection of these strains in clinical laboratories an essential step. The frequency of genes encoded ESBL isolates of Enterobacter clocae, Acinetobacter baumannii and Proteus mirabilis may be due to abuse and misuse of antibiotics.

Investigation of carbapenem resistant Acinetobacter baumannii ST2 in Iran

2020 ◽  
Author(s):  
Aliakbar Rezaei ◽  
Hossein Fazeli ◽  
Jamshid Faghri
Keyword(s):  
Acinetobacter Baumannii ◽  
Clonal Complex ◽  
Carbapenem Resistance ◽  
Diffusion Method ◽  
Disc Diffusion Method ◽  
Carbapenem Resistant ◽  
Rapd Pcr ◽  
Relationship Of ◽  
Sequence Types ◽  
Respiratory Specimens

AbstractThis study investigated carbapenem resistance among Acinetobacter baumannii isolated from respiratory specimens. Epidemiological relationship of the isolates was also evaluated. In this study, 81 respiratory specimens of A. baumannii from AL Zahra Hospital were confirmed by phenotypic and genotypic methods. Antimicrobial susceptibility was performed by disc diffusion method. Carbapenem resistance genes were identified by PCR. The isolates were typed by RAPD-PCR and multilocus sequence typing (MLST) methods. All isolates were resistant to imipenem and 80 isolates to meropenem. Frequency of oxacillinase genes was as follows: blaOXA-23 gene was positive in 74 (91.3%), blaOXA-24 gene in 50 (61.7%) and blaOXA-58 was not found in any isolates. On the other hand 22 (27.2%) isolates contained blaIMP-1, 3 (3.7%) isolates contained blaIMP-2 gene, 5 (6.2%) isolates contained blaVIM-1, 4 (5%) isolates had blaVIM-2 and none of the isolates had blaSIM-1 gene. RAPD-PCR typing identified 16 different patterns, with one pattern being the most frequent one in 26 isolates. In MLST 6 different sequence types were identified, the most predominant being ST2 belonging to clonal complex 2. The results of this study showed high resistance to carbapenems as well as high abundance of oxacillinase genes.

scholarly journals Distribution and Molecular Characterization of Acinetobacter baumannii International Clone II Lineage in Japan

2017 ◽  
Vol 62 (2) ◽  
Author(s):  
Mari Matsui ◽  
Masato Suzuki ◽  
Masahiro Suzuki ◽  
Jun Yatsuyanagi ◽  
Masanori Watahiki ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Fluoroquinolone Resistance ◽  
Content Type ◽  
Low Prevalence ◽  
Prevalent Species ◽  
Sequence Types ◽  
Insight Into

ABSTRACTMultidrug-resistant (MDR)Acinetobacterspp. have been globally disseminated in association with the successful clonal lineageAcinetobacter baumanniiinternational clone II (IC II). Because the prevalence of MDRAcinetobacterspp. in Japan remains very low, we characterized allAcinetobacterspp. (n= 866) from 76 hospitals between October 2012 and March 2013 to describe the entire molecular epidemiology ofAcinetobacterspp. The most prevalent species wasA. baumannii(n= 645; 74.5%), withA. baumanniiIC II (n= 245) accounting for 28.3% of the total. Meropenem-resistant isolates accounted for 2.0% (n= 17) and carried ISAba1-blaOXA-23-like(n= 10),blaIMP(n= 4), or ISAba1-blaOXA-51-like(n= 3). Multilocus sequence typing of 110 representativeA. baumanniiisolates revealed the considerable prevalence of domestic sequence types (STs).A. baumanniiIC II isolates were divided into the domestic sequence type 469 (ST469) (n= 18) and the globally disseminated STs ST208 (n= 14) and ST219 (n= 4). ST469 isolates were susceptible to more antimicrobial agents, while ST208 and ST219 overproduced the intrinsic AmpC β-lactamase.A. baumanniiIC II and someA. baumanniinon-IC II STs (e.g., ST149 and ST246) were associated with fluoroquinolone resistance. This study revealed that carbapenem-susceptibleA. baumanniiIC II was moderately disseminated in Japan. The low prevalence of acquired carbapenemase genes and presence of domestic STs could contribute to the low prevalence of MDRA. baumannii. A similar epidemiology might have appeared before the global dissemination of MDR epidemic lineages. In addition, fluoroquinolone resistance associated withA. baumanniiIC II may provide insight into the significance ofA. baumanniiepidemic clones.

scholarly journals A comparative analysis on safety culture in domestic and foreign health care facilities and enterprises of other activities (message 2)

2021 ◽  
Vol 26 (4) ◽  
pp. 153-160
Author(s):  
A.P. Yavorovsky ◽  
M.M. Rygan ◽  
A.N. Naumenko ◽  
Yu.N. Skaletsky ◽  
S.G. Gichka ◽  
...  
Keyword(s):  
Health Care ◽  
Patient Safety ◽  
Safety Culture ◽  
Nuclear Power ◽  
Power Plants ◽  
Nuclear Power Plants ◽  
Real Data ◽  
Health Care Facilities ◽  
Hospital Environment ◽  
Care Facilities

The characteristics of the safety culture of patients and personnel in health care facilities in Ukraine as a whole and separately among doctors-pathologists are analyzed with correlation of the data obtained with similar indicators of the culture of patient safety in medical facilities of other countries and comparison with the safety culture of workers of domestic nuclear power plants. It was confirmed that the weaknesses of the safety culture of the personnel of domestic hospitals is characterized by "Reaction to mistakes", which indicates the prevalence of the culture of blame (unfair culture) in domestic hospitals and, as a result, the absence of real data on medical errors and other incidents of patient safety. The high percentage of positive responses to the safety culture characteristic “Response to mistakes” among the workers of Ukrainian nuclear power plants is an example of the possibility of forming an appropriate safety culture in a separate domestic industry, and the high percentage of positive answers by this characteristic in domestic pathologists is a significant potential for the development of a safe hospital environment for patients. in Ukraine.

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