Root Development of Bean (Phaseolus vulgaris L.) is Affected by Trifluralin Herbicide
The objectives of this study were to investigate the effects of trifluralin (TRI) herbicide on plant vigor and lateral root growth of snap beans. Lexington silt loam soil, which had not been treated with herbicides for at least 3 years, was used in this study. TRI herbicide was mixed with appropriate amounts of soil in a cement mixer to provide a constant rate of 560 g ai/ha TRI. To simulate TRI herbicide incorporation, bands of treated and untreated soil were layered into 30 × 30 × 15 cm (vol 13,500 cm3) bottomless wood boxes. Treatments were 1) control, 2) 2.5-cm treated band placed 2.5 cm below soil surface (BSS), 3) 2.5 cm treated band placed 5.1 cm BSS, 4) top 5.1 cm with treated soil, or 5) top 7.6 cm with treated soil. `Benton' snap beans were planted in each box and grown in the greenhouse for 25 days. Plant measurements made included plant height, leaf area, and fresh and dry weight. The root system of each plant was washed and roots arising from the hypocotyl, transition zone, and primary roots were counted. All roots from each box were combined, dried overnight at 100C and weighed. Plant top weight and leaf area were not affected by TRI treatments. Plants were shorter in all TRI treatments except where placement was banded at 5.1 cm BSS. More adventitious roots were produced on hypocotyls when TRI was applied in a 2.5-cm band at 2.5-cm BSS or in the top 5.1 cm of soil. Total root weight was lower and fewer lateral roots developed in all soil bands treated with TRI.