scholarly journals Germination Temperature and the Effect of Storage Time on the Seed Viability of Zephyra elegans (Tecophilaeaceae)

HortScience ◽  
2018 ◽  
Vol 53 (6) ◽  
pp. 887-890
Author(s):  
Carlos De la Cuadra ◽  
Alexis K. Vidal ◽  
Patricia Peñaloza ◽  
Leví Mansur ◽  
Carlos Huenchuleo
Keyword(s):  
Room Temperature ◽  
Natural Habitat ◽  
Seed Viability ◽  
Dry Storage ◽  
High Viability ◽  
High Germination ◽  
Temperature Requirements ◽  
Variable Precipitation ◽  
Storage Times ◽  
Desert Climate

Zephyra elegans is a geophyte endemic to Chile that has horticultural value as a cut flower. Its natural habitat is a coastal desert climate with abundant cloud cover, little and variable precipitation, and mean monthly temperatures between 10 and 20 °C. It is hypothesized that the temperature requirements for germination of Z. elegans seeds are similar to those of Zephyra compacta, which shares the same habitat. As it is a species from a desert climate, it can be expected that its seeds remain viable during long periods of storage. The seeds used in this study were harvested in different years from plants grown from cultivated corms of Z. elegans. The germination test temperatures were 10, 15, 20, and 25 °C, and the dry storage times at room temperature (20 ± 5 °C) were 4, 16, 28, and 40 months. The temperature requirements for germination of Z. elegans seeds are similar to those of Z. compacta, which has been studied earlier, i.e., the temperature range for achieving high germination percentages is 10 to 20 °C, whereas 25 °C can be considered supraoptimal. After-ripening of the seeds occurred during dry storage, as shown by increased germination when tested at a supraoptimal temperature. The seeds had high viability after 40 months of dry storage at room temperature.

scholarly journals BIOCHEMICAL ANALYSES OF SEED EXUDATES DURING EARLY IMBIBITION

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 684g-685
Author(s):  
Eric E. Roos ◽  
Sharon Sowa ◽  
Kristin Pedas
Keyword(s):  
Oryza Sativa ◽  
Protein Concentration ◽  
Room Temperature ◽  
Seed Viability ◽  
Distilled Water ◽  
Short Term ◽  
Time Period ◽  
High Viability ◽  
Biochemical Analyses ◽  
Bca Assay

Short term soaking of seeds does not appear to be detrimental to seed viability and may provide a means of testing seed viability non-destructively. Seeds of corn (Zea mays L.) and rice (Oryza sativa L.), differing in viability, were soaked for 0.5, 1, 2 and 4 hr in distilled water at room temperature. Analyses of pH, protein/polypeptides (BCA assay and absorbance at 280 nm), and potassium (and other metals), were done on individual seed leachates. After each time period seeds were germinated for 7 d to determine viability. For both corn and rice, pH remained constant between 0.5 and 4 hr of soaking. Protein concentration gradually increased during the 4 hr soak in both corn and rice, but varied with seed lot. Potassium was the most common metal excreted and increased 3 to 4 fold between 0.5 and 4 hr of soaking. Although seed to seed variability in any given lot was high, in general, low viability seeds lost more cellular constituents than high viability seeds.

scholarly journals Effect of common bean (Phaseolus vulgaris L.) seed viability on drying dynamics

2008 ◽  
Vol 20 (2) ◽  
pp. 85-92 ◽  
Author(s):  
Krzysztof Ratajczak ◽  
Roman Hołubowicz
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Seed Viability ◽  
Artificial Ageing ◽  
Germination Capacity ◽  
Phaseolus Vulgaris L ◽  
High Viability ◽  
High Germination ◽  
Thermal Chamber ◽  
The Common

Abstract ‘Tina’ and ‘Libra’ cultivar seeds of the common bean (Phaseolus vulgaris L.) beginning germination capacity of 98%, were subjected to artificial ageing. In the thermal chamber, they were twice treated for 72 hours at 45°C. After the treatment, the seeds had lower viability. They germinated at 12% and 0% for ‘Libra’ and ‘Tina’ cultivars, respectively. The dynamics and rate of seed drying were measured in samples with high and low viability. It was found that the seeds of both cultivars with high viability, expressed by high germination capacity, dried at a slower rate than ones with low viability. In the future, this method of estimating the drying rate could probably be used to test seeds with different degrees of viability.

scholarly journals Seed productivity and dynamics of germination of Ziziphora L. species in the Pamir-Alay

2018 ◽  
Vol 11 ◽  
pp. 00006
Author(s):  
Kobil Bobokalonov ◽  
Vera Cheryomushkina
Keyword(s):  
Seed Viability ◽  
Seed Productivity ◽  
Germination Energy ◽  
Dormancy Period ◽  
High Viability ◽  
High Germination

Seed productivity of 4 Ziziphora species: Z. pamiroalaica, Z. interrupta, Z. brevicalyx, and Z. suffruticosa growing in the Pamir-Alay Mountains was stUdied. It was revealed that the indicator of real seed productivity did not exceed 40 %. Seed viability and character of germination were studied. It was established that seeds of all species did not have a dormancy period, had high viability, up to 95 % and high germination energy.

scholarly journals Pollination of Dendrobium Hybrids Using Cryopreserved Pollen

HortScience ◽  
2008 ◽  
Vol 43 (1) ◽  
pp. 264-267 ◽  
Author(s):  
Wagner A. Vendrame ◽  
Virginia S. Carvalho ◽  
José M.M. Dias ◽  
Ian Maguire
Keyword(s):  
Liquid Nitrogen ◽  
Room Temperature ◽  
Seed Viability ◽  
Viability Test ◽  
Pollination Effectiveness ◽  
High Viability ◽  
Leaves And Roots ◽  
Germinating Seeds

Pollination effectiveness was evaluated for pollen (pollinia) from two Dendrobium hybrids, ‘Sena Red’ and ‘Mini WRL’, submitted to cryopreservation using a vitrification protocol. Parameters evaluated included pollinia exposure to a previtrification solution (PVS2) under ice (0 °C) or room (27 ± 2 °C) temperatures from 1 to 4 hours before cryopreservation (LN). On removal from cryopreservation, pollinia were used to pollinate flowers of the same hybrids to verify viability and germination. All pollinia showed high percentages of germination (greater than 80%) after crosses were performed, except for pollinia from Dendrobium ‘Sena Red’ submitted to 3 hours of precooling (0 °C) in PVS2 followed by LN (60%) and for pollinia submitted to PVS2 for 3 hours at room temperature with no precooling (70%). Capsules were formed for both hybrids and seeds were successfully produced. The seed viability test revealed high viability (90% to 95%) for all treatments for both hybrids. Seeds observed under a microscope contained well-formed embryos and no abnormalities were identified. Seeds from all treatments germinated. Germinating seeds developed into healthy seedlings with well-formed leaves and roots. Cryopreservation of pollinia was successfully accomplished either by direct storage in liquid nitrogen without cryoprotection treatments or by using a PVS2 vitrification protocol.

scholarly journals Seed germination requirements of Hypericum scruglii, an endangered medicinal plant species of Sardinia (Italy)

Botany ◽  
2020 ◽  
Vol 98 (10) ◽  
pp. 615-621 ◽  
Author(s):  
Marco Porceddu ◽  
Martina Sanna ◽  
Sara Serra ◽  
Maria Manconi ◽  
Gianluigi Bacchetta
Keyword(s):  
Medicinal Plant ◽  
Seed Bank ◽  
Seed Viability ◽  
Base Temperature ◽  
Endemic Plant ◽  
Endangered Medicinal Plant ◽  
High Viability ◽  
Wide Range ◽  
Baseline Information ◽  
Germination Requirements

Hypericum scruglii Bacch., Brullo & Salmeri is an endangered endemic plant of Sardinia, and the phloroglucinol compounds identified in this species have been reported to inhibit the activity of the Human Immunodeficiency Virus. Seed banks are a genetic repository that effectively preserve taxa of conservation interest, and they hold knowledge about the biology and germination ecophysiology of the taxa they preserve. The main goals of this study were to investigate the germination requirements for H. scruglii, to evaluate the seed viability after eight years of long-term conservation and to suggest an efficient protocol for germination. Seeds stored at –25 °C in the seed bank were tested at temperatures from 5 to 30 °C and 25/10 °C. Base temperature (Tb) and thermal time (θ50) for germination were estimated. Seeds of H. scruglii germinated over a wide range of temperatures, responding positively to high temperatures and 25/10 °C. Tb was estimated at 4.92 °C, and θ50 was estimated at 198.27 degree days. The high viability detected in seeds of H. scruglii allows us to suggest the use of seeds stored in a seed bank when fresh material for plant propagation is scarce. Our results provide new and useful baseline information for implementing conservation and multiplication strategies for this endangered medicinal plant.

scholarly journals Materials for Future Quantum Dot-Based Memories

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
T. Nowozin ◽  
D. Bimberg ◽  
K. Daqrouq ◽  
M. N. Ajour ◽  
M. Awedh
Keyword(s):  
Cross Sections ◽  
Room Temperature ◽  
Storage Time ◽  
Thermal Emission ◽  
Current Status ◽  
Strong Electron ◽  
Self Organized ◽  
Hole Confinement ◽  
Storage Times ◽  
Capture Cross Sections

The present paper investigates the current status of the storage times in self-organized QDs, surveying a variety of heterostructures advantageous for strong electron and/or hole confinement. Experimental data for the electronic properties, such as localization energies and capture cross-sections, are listed. Based on the theory of thermal emission of carriers from QDs, we extrapolate the values for materials that would increase the storage time at room temperature to more than millions of years. For electron storage, GaSb/AlSb, GaN/AlN, and InAs/AlSb are proposed. For hole storage, GaSb/Al0.9Ga0.1As, GaSb/GaP, and GaSb/AlP are promising candidates.

scholarly journals Biological features of seeds of a wild-growing species of Poterium polygamum

2022 ◽  
Vol 354 (11-12) ◽  
pp. 97-99
Author(s):  
M. A. Starostina ◽  
N. G. Lapenko
Keyword(s):  
Natural Population ◽  
Seed Viability ◽  
Storage Period ◽  
Seed Storage ◽  
Scientific Basis ◽  
The Third ◽  
Biological Features ◽  
High Germination ◽  
Grass Stand ◽  
The Subject

Relevance. Biological features (morphometric indicators, viability and germinating power) of Poterium polygamum seeds found in natural communites of the Stavropol Territory have been studied. The conservation of wild-growing plant species in the natural population and the rational use of their potential are relevant.Methods. Years of study — 2013–2021. The subject of the study is Poterium polygamum seeds. Seeds were collected in 2013. Studies were carried out according to the methods of studying seed material. Freshly harvested seeds and seeds of different shelf life were germinated in petri dishes at a temperature of 18–20 °C.Results. The collected seeds of Poterium polygamum are quite large. The length of the seeds is 4–5 mm, the width is about 2.5 mm. The mass of 1000 pieces of seeds on average was 10.59 grams. Complete absence of viability of freshly harvested Poterium polygamum seeds was revealed. After three months, seed viability was 97%. This is a shallow physiological rest associated with the post-harvest maturation period. During the storage of seeds, their high germination was established — from 70 to 100%. The duration of seed storage is 8 years. The largest number of germinated seeds and their germinating power were noted in the third year of their storage (100%). Seeds germinated on the third or fourth day. The differences between the viability of seeds and their germinating power are not great. In the eighth year of storage of Poterium polygamum seeds, viability and germinating power were slightly reduced. The storage period of seeds, in which they retain the ability to germinate and the germinating power, is long. It has been found that the wild species Poterium polygamum belongs to plants with a high (70 to 100%) viability. This species is regularly resumed in the natural population. The revealed biological features of Poterium polygamum seeds can become the scientific basis during the reconstruction of degraded natural grass stand and in selection work.

scholarly journals Viability of recalcitrant Araucaria angustifolia seeds in storage and in a soil seed bank

2019 ◽  
Vol 31 (6) ◽  
pp. 2413-2422 ◽  
Author(s):  
Ezequiel Gasparin ◽  
José M. R. Faria ◽  
Anderson C. José ◽  
Olivia A. O. Tonetti ◽  
Rodrigo A. de Melo ◽  
...  
Keyword(s):  
Water Content ◽  
Seed Bank ◽  
Forest Cover ◽  
Soil Seed Bank ◽  
Natural Habitat ◽  
Seed Viability ◽  
Germplasm Conservation ◽  
Forest Edge ◽  
Araucaria Angustifolia ◽  
Subsequent Decrease

Abstract Araucaria angustifolia (Bertol.) Kuntze is a representative species of the Mixed Ombrophilous Forest in the Atlantic Forest Biome of Brazil. The development of a germplasm conservation protocol for long-term seed bank storage is compromised for this species, as it is sensitive to desiccation. Furthermore, in situ establishment of a soil seed bank in its natural habitat may be limited. This study evaluates the storability of two provenances of A. angustifolia seeds and their behavior in an artificial soil seed bank in two forest environments (understory and edge). Results show that both seed provenances may be stored at 5 °C for approximately 12 months, retaining high viability. The subsequent decrease in germination was associated with a reduction and an increase in seed water content, as well as with increased electrical conductivity. In the understory environment, seed viability was above 85% for the first 60 days, and at the end of the experiment (270 days), seedlings emerged. However, at the forest edge, there was a total loss of seed viability after 120 days associated with a reduction in water content and high predation. It is concluded, therefore, that short-term storage of A. angustifolia seeds is possible in a cold room, which is fundamental to supply seed demand outside the production period. Forest cover conservation is important for regeneration and conservation of the species.

Purple prairie clover seed viability and germinability after passing through the digestive tracts of yearling steers

2019 ◽  
Vol 99 (5) ◽  
pp. 734-739
Author(s):  
Chen Gu ◽  
Alan D. Iwaasa ◽  
Mengli Zhao
Keyword(s):  
Low Cost ◽  
Seed Viability ◽  
Germination Percentage ◽  
Cattle Dung ◽  
Western Canada ◽  
Important Method ◽  
Hard Seed ◽  
Native North American ◽  
High Viability ◽  
Clover Seed

Endozoochory by cattle has been considered an important method for population self-regeneration; however, the effects of cattle digestive tracts on the viability and germinability of purple prairie clover (Dalea purpurea Vent.), a native North American species, remain understudied. The experiment was conducted at the Agriculture and Agri-Food Canada Swift Current Research and Development Centre in the semiarid prairie of western Canada. Purple prairie clover seeds extracted from cattle dung pats were tested for germination and compared with uningested and scarified seeds from the same paddock. The germination percentage (within 200 d) of the ingested seeds was 16.5% ± 0.8%, two times higher after passing through digestive tracts than uningested seeds (8.3% ± 1.2%), while 76.6% ± 1.5% of ingested seeds remained dormant. As many as 92.4% ± 0.9% of purple prairie clover seeds survived digestion due to their high hard seed rate (90.5% ± 1.0%), which was lower than the uningested seeds by 6.9%. Therefore, cattle dispersing purple prairie clover seeds with high viability and germinability is a low-cost method to increase and rejuvenate purple prairie clover for late-season grazing pastures.

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