scholarly journals Indole -3- Butyric Acid Induces Plant Regeneration From Stem Cuttings Of Three Medicinal Plants

2020 ◽  
Vol 37 (1) ◽  
pp. 109-121
Author(s):  
C.U. Okafor ◽  
E.U. Njoku ◽  
F.C. Ike ◽  
C.C. Onyekwuluje
Keyword(s):  
Medicinal Plants ◽  
Butyric Acid ◽  
Survival Rates ◽  
Control Treatment ◽  
Stem Cuttings ◽  
Root Number ◽  
Growth And Survival ◽  
Root Numbers ◽  
Significant Difference ◽  
Leaf Formation

Field regeneration of three (3) medicinal plants - Securidaca longepedunculata (violet tree), Ocimum gratissimum (scent leaf) and Pterocarpus mildebraedii by means of rooting of stem cuttings at different lengths of 10 -15 cm and 15 -20 cm were studied. The effect of the plant hormone Indole-3-Butyric acid (IBA) at 5g/l was tested on rooting, bud sprout and leaf formation of the rooted stem cuttings of the plant species as well as the effect of the length of the stem cuttings on their growth and survival rates. The results of the study revealed that stem cuttings of O. gratissimum treated with IBA and the control treatment sprouted within 5 - 8 days. The treated O. gratissimum stem cuttings produced a slightly higher number of buds (2.58 ± 0.86) when compared to the control (2.00 ± 0.89) with no significant difference at P ≤ 0.05. Stem cuttings of the treated P. mildbraedii cuttings sprouted within 8 -10 days with 1.40 ± 0.37 number of buds, with the control showing no sprouts. Consequently, IBA application produced more leaves (13.00 ± 5.58) and roots (1.33 ± 0.01) than the control. However, O. gratissimum stem cuttings had the highest leaf (13.08 ± 4.47) and root numbers (135.00 ± 13.45) followed by the control with values- 13.00 ± 5.58 for leaf development and 61.66 ± 2.34 for root number respectively. Irrespective of the treatments, none of the stem cuttings of S. longepedunculata sprouted. This study showed that exogenous application of IBA to stem cuttings of the tested plants, except S. Longepedunculata, improved the root number, number of leaves and number of buds. Keywords: Stem cuttings, Indole-3-Butyric acid (IBA), Rooting

scholarly journals Three Mussaenda Cultivars Propagated by Stem Cuttings Exhibit Variation in Rooting in Response to Hormone and Rooting Conditions

2000 ◽  
Vol 10 (4) ◽  
pp. 780-784
Author(s):  
Rolston St. Hilaire ◽  
Carlos A. Fierro Berwart
Keyword(s):  
Butyric Acid ◽  
Root Length ◽  
Clonal Propagation ◽  
Stem Cuttings ◽  
Root Number ◽  
Root Numbers ◽  
Rooting Medium ◽  
Rooting Of Cuttings

The effects of 1H-indole-3-butyric acid (IBA), cutting position on stock plants, the date of propagation, the type of rooting substrate and temperature on rooting of mussaenda (Mussaenda erythrophylla Schumach. & Thonn. `Ashanti Blood' and `Rosea', and Mussaenda philippica A. Rich `Aurorae') stem cuttings were determined. Cuttings of `Ashanti Blood' produced the largest number of roots when treated with 15 mmol (3000 ppm) IBA and rooted in perlite at 29 °C (84 °F). Cutting position on stock plants did not affect rooting in any of the three cultivars. Propagation date and temperature of the rooting medium affected root numbers in `Aurorae'. With `Rosea', only the type of rooting substrate affected root number. Rooting percentage was 22%, 48%, and 39% in `Ashanti Blood', `Aurorae', and `Rosea' respectively. After 30 days of propagation average root length was 4, 12, and 4 mm (0.2, 0.5, and 0.2 inch) in `Ashanti Blood', `Aurorae', and `Rosea' respectively. Growers must determine precise rooting conditions for each cultivar to obtain consistent rooting of cuttings. This process may not be economically feasible on a commercial basis because rooting percentages are relatively low. We conclude that other methods of clonal propagation need to be evaluated before uniform rooted stem cuttings of mussaenda can be produced economically.

scholarly journals Study of indole butyric acid (IBA) effects on cutting rooting improving some of wild genotypes of damask roses (Rosa damascena Mill.)

2015 ◽  
Vol 60 (3) ◽  
pp. 263-275 ◽  
Author(s):  
Fardin Nasri ◽  
Arsalan Fadakar ◽  
Mahmood Saba ◽  
Bayzid Yousefi
Keyword(s):  
Butyric Acid ◽  
Control Treatment ◽  
Rosa Damascena ◽  
Indole Butyric Acid ◽  
Significant Difference ◽  
Maximum Root Length ◽  
Number Of Leaves ◽  
Maximum Root ◽  
Medicinal Properties ◽  
The Rose

Rosa damascena is very important for essential oil production, medicinal properties and it is also widely cultivated as a garden rose. The Rose species is mainly propagated by stem cutting. In the present study, the effect of different levels of 0, 500 and 1,000 mg l-1 (quick dip method for 20 s) of indole butyric acid (IBA) on the rooting of 12 wild genotypes (including: Kurdistan 1 to Kurdistan 12) of R. damascena was investigated. The results show that the rooting ability of R. damascena differs significantly between the twelve genotypes. The highest rooting (79.56%) and callus production (69.08%), number of roots (8.33), root fresh and dry weights (361.80 and 244.74 mg, respectively) were recorded in Kurdistan 5 genotype with 1,000 mg l-1 IBA. The maximum root length (5.84 cm) was observed in Kurdistan 5 genotype with 500 mg l-1 IBA that showed a significant difference compared to the control treatment (0.96 cm). The highest number of leaves per bud (7.33 at 500 mg l-1 IBA) and number of buds (5.00 at 1,000 mg l-1 IBA) were recorded in Kurdistan 1 genotype. The current study demonstrated that the different genotypes of R. damascena were in a difficult-toroot state, which suggests that cutting treatment with 1,000 mg l-1 IBA overcame the problem of the difficult-to-root state, and it can also enhance the rooting percentage in the studied genotypes.

scholarly journals Role of micro and macronutrients enrich fertilizers on the growth performance of prawn (Macrobrachium rosenbergii), rohu (Labeo rohita) and mola (Amblypharyngodon mola) in a polyculture system

2018 ◽  
Vol 8 (2) ◽  
pp. 47-53
Author(s):  
MM Rahman ◽  
S Sultana ◽  
M Kabiraj ◽  
M Das
Keyword(s):  
Growth Performance ◽  
Labeo Rohita ◽  
Survival Rates ◽  
Macrobrachium Rosenbergii ◽  
Quality Parameters ◽  
Mean Value ◽  
Control Treatment ◽  
Significant Difference ◽  
Level Of Significance

A six month’s experiment was carried out in earthen ponds to evaluate the effect of micro and macro nutrients on the growth performance of Prawn (Macrobrachium rosenbergii), Rohu (Labeo rohita) and Mola (Amblypharyngodon mola) in a polyculture system during July to December 2017. The trial was conducted into two different treatments and one control each with three replications. In treatment-1, macronutrients enrich fertilizers like urea, TSP and molasses-yeast mixture were applied at the rate of 2.45, 2.45, and 3.09 (g m-2 week-1), whereas in treatment-2 micronutrients composition mixer applied at the rate of 4.50 g m-2 week-1 while no micro and macronutrients were applied in control. The mean value of all water quality parameters showed no significant differences among control and two treatments when One-way ANOVA was performed except hardness. The average final mean individual weights of prawn, rohu and mola in control, treatment-1 and treatment-2 were 22.10, 24.35, and 24.70 g; 141.10, 190.60 and 182.20 g; 3.36, 3.67 and 3.73 g, respectively. The survival rates of various species namely prawn, rohu and mola in control, treatment-1 and treatment-2 were 73.33, 89.58 and 86.25%; 78.00, 74.00 and 74.00%; 10.67, 15.33 and 15.33%, respectively. There was no significant difference in the survival rates of prawn, rohu and mola among control and treatments. Production after six months of culture was 351.8, 461.5 and 445.3 kg ha-1 in control, treatment-1 and treatment-2, respectively which were significantly different at 5% level of significance. Int. J. Agril. Res. Innov. & Tech. 8 (2): 47-53, December, 2018

scholarly journals Effects of Auxin Concentration and Medium Temperature on Four Woody Plant Taxa Propagated by Stem Cuttings using Recirculating Subirrigation in a Growth Chamber

2003 ◽  
Vol 21 (3) ◽  
pp. 126-130
Author(s):  
James S. Owen ◽  
William A. Johnson ◽  
Brian K. Maynard
Keyword(s):  
Root Formation ◽  
Woody Plant ◽  
Adventitious Root Formation ◽  
Stem Cuttings ◽  
Root Number ◽  
Medium Temperature ◽  
Root Numbers ◽  
Auxin Treatment ◽  
Auxin Concentration ◽  
Propagation Medium

Abstract Four woody plant taxa (‘Sparkleberry’ holly, ‘Mariesii’ viburnum, ‘Shasta’ viburnum, and Red Sunset® maple) were propagated from softwood stem cuttings in a recirculating subirrigation propagation system to evaluate the effect on adventitious root formation of three auxin (Dip-n-Grow®) concentrations [0 (water), 20: 1, or 10: 1] and three medium temperatures [20C (68F), 23C (74F), or 26C (79F)]. All taxa showed a positive rooting response to auxin treatment. Compared to cuttings treated with water, root number of all taxa doubled when treated with the highest auxin concentration (10: 1 Dip-n-Grow® dilution). Length of the longest root of rooted cuttings increased with auxin treatment of two taxa. Medium temperature also affected rooting of all taxa. The intermediate temperature evaluated, 23C (74F), was optimal for all rooting parameters on cuttings of ‘Mariesii’ viburnum, and for root length and percentage rooting on cuttings of ‘Shasta’ viburnum and Red Sunset® maple. A propagation medium temperature of 26C (79F) resulted in the greatest root number on cuttings of ‘Shasta’ viburnum, while 20C (68F) was optimal for root number on cuttings of Red Sunset® maple. Root numbers on cuttings of ‘Mariesii’ viburnum were reduced at 20C (68F) and 26C (79F), which appeared to be sub-and supra-optimal temperatures, respectively, for root initiation. All rooting parameters on cuttings of ‘Sparkleberry’ holly increased with propagation medium temperature. Temperature-auxin interactions were apparent for root number and length on cuttings of ‘Mariesii’ and ‘Shasta’ viburnum. The recirculating subirrigation system was useful for evaluating the basal temperature and auxin dose interactions and was effective for rooting softwood stem cuttings without intermittent mist irrigation.

scholarly journals Indolebutyric acid on boxwood propagation by stem cuttings

2018 ◽  
Vol 24 (4) ◽  
pp. 347-352 ◽  
Author(s):  
Leandro Marcolino Vieira ◽  
Silvano Kruchelski ◽  
Erik Nunes Gomes ◽  
Katia Christina Zuffellato-Ribas
Keyword(s):  
Statistical Difference ◽  
Callus Formation ◽  
Adventitious Rooting ◽  
Commercial Production ◽  
Stem Cuttings ◽  
Root Number ◽  
Indolebutyric Acid ◽  
Significant Percentage ◽  
Root Numbers ◽  
Buxus Sempervirens

Buxus sempervirens L., popularly known as boxwood, is a woody shrub widely used in gardening and landscaping. One of the most important factors for B. sempervirens commercial production is its propagation. The objective of this study was to evaluate the effect of indolebutyric acid (IBA) concentrations on the adventitious rooting of semihardwood stem cuttings of the species. Branches with new shoots were collected from stock plants with approximately 17 years of growth. Stem cuttings of six centimeters in length with two leaves in the upper region were made with a straight cut at the apex and a bevel cut at the base. Cuttings bases were immersed for 10 seconds in hydroalcoholic solutions (50% v v-1) with IBA at 0, 1500, 3,000 or 6,000 mg L-1. The cuttings were evaluated at 64 and 116 days after planting. Rooting, mortality, callus formation and sprouting percentages as well as root number and root length were measured. At 64 days, no significant percentage of rooting was observed in B. sempervirens stem cuttings and sprouting and callus formation were not significantly affected by the treatments. Keeping the cuttings in the greenhouse for 116 days led to rooting rates up to a 97.5%, with no statistical difference among treatments. Root lengths and root numbers did not differ as a function of treatments. The required period for B. sempervirens stem cuttings rooting is around 116 days; IBA application does not affect rooting, survival or sprouting of B. sempervirens stem cuttings.

scholarly journals CRESCIMENTO E SOBREVIVÊNCIA DE QUATRO ESPÉCIES FLORESTAIS NATIVAS PLANTADAS EM ÁREAS COM DIFERENTES ESTÁGIOS DE SUCESSÃO NO RESERVATÓRIO IRAÍ-PR

FLORESTA ◽  
2011 ◽  
Vol 41 (4) ◽  
Author(s):  
Larissa De Bortolli Chiamolera ◽  
Alessandro Camargo Angelo ◽  
Maria Regina Boeger
Keyword(s):  
Native Species ◽  
Riparian Forest ◽  
Survival Rates ◽  
Water Reservoir ◽  
Open Area ◽  
Schinus Terebinthifolius ◽  
Growth And Survival ◽  
Significant Difference ◽  
Native Tree ◽  
Native Tree Species

O objetivo deste trabalho foi avaliar o crescimento e a sobrevivência de Schinus terebinthifolius, Mimosa scabrella, Podocarpus lambertii e Luehea divaricata plantadas em áreas com diferentes graus de sucessão, nas margens do Reservatório do Iraí - PR. S. terebinthifolius apresentou uma superioridade de crescimento entre os indivíduos que estavam na capoeira até os 18 meses, não diferindo mais aos 24 meses, com taxa de sobrevivência, aos 24 meses de plantio, de 81,37% para a área de capoeira e 68,15% para a área aberta. M. scabrella foi a espécie que obteve os piores índices de sobrevivência em ambas as áreas, restando, aos 24 meses, na área aberta, 13,54% das mudas implantadas, e na capoeira, 21,18%. P. lambertii apresentou sempre melhores índices de crescimento na área aberta. L. divaricata, até os 18 meses, não apresentou diferença significativa entre a área aberta e a capoeira, porém, aos 24 meses, a altura foi maior na área de capoeira e para o diâmetro foi maior na área aberta. Assim, entre as espécies estudadas, S. terebinthifolius é a espécie com maior plasticidade fenotípica, seguida por P. lambertii. L. divaricata deve ser priorizada em plantios que ofereçam maior sombreamento e M. scabrella é recomendada somente em altas densidades.Palavras-chave: Silvicultura de espécies nativas; recuperação de ambientes ciliares. AbstractGrowth and survival of four forest native species growing in areas with diferent sucessional status on Iraí’s Reservoir margins.The objective of this study was to evaluate the growth and survival of Schinus terebinthifolius, Mimosa scabrella, Podocarpus lambertii and Luehea divaricata planted in two areas with different degrees of succession, in Iraí’s water reservoir margins, State of Paraná, Brazil.S. terebinthifolius showed a superiority of the individuals who were in the regenerating area up to 18 months did not differ at 24 months and their survival rate at 24 months after planting, was 81.37% for regenerating area and 68.15% for the open area. M. scabrella was the kind that had the poorest survival rates in both areas, leaving at 24 months in the open area 13.54% of the seedlings established and regenerating area, 21.18%. P. lambertii always had the best growth rates in the open area. L. divaricata up to 18 months showed no significant difference between the open area and regenerating area, but at 24 months the time was higher for regenerating area and the diameter was greater in the open area. Thus, among the species S. terebinthifolius is the species with greater plasticity followed by P. lambertii. L. divaricata should be prioritized in plantations to provide greater shading and M. scabrella is recommended only in high densities.Keywords: Native tree species silviculture; riparian forest recuperation.

scholarly journals Exogenous Treatments to Enhance Splice-Grafted Watermelon Survival

Horticulturae ◽  
2021 ◽  
Vol 7 (7) ◽  
pp. 197
Author(s):  
Pinki Devi ◽  
Lisa Wasko DeVetter ◽  
Scott Lukas ◽  
Carol Miles
Keyword(s):  
Graft Survival ◽  
Butyric Acid ◽  
Cost Effective ◽  
Control Treatment ◽  
Water Control ◽  
External Application ◽  
Intact Plants ◽  
Significant Difference

This study evaluated the use of splice grafting as a propagation strategy for watermelon. In experiment 1, the treatments consisted of sucrose, antitranspirant A, antitranspirant B, auxin (indole-3-butyric acid (IBA)) at two concentrations (10 and 20 mgL−1), plus a water control. The survival (%) of splice-grafted watermelon plants differed due to the number of days after grafting and treatment (p < 0.0001, for both). At 21 days after grafting, plants treated with sucrose and antitranspirant A, and sucrose and antitranspirant A with 10 mgL−1 auxin had 90% and 88% survival, respectively, whereas the graft survival was 18% for plants treated with water. Experiment 2 included the three top performing treatments from experiment 1 and a water control treatment, applied to both root-intact and root-excised rootstocks. There was a significant difference in survival (%) of splice-grafted watermelon due to root treatments, exogenous treatments, and the number of days after grafting (p < 0.0001, for all). At 21 days after grafting, survival for root-excised grafted plants was 11% lower compared to root-intact plants. Plants treated with sucrose and antitranspirant A, and sucrose and antitranspirant A with 10 mgL−1 auxin had 87% and 86% survival, respectively, whereas plants treated with water had 14% survival. The external application of auxin applied to rootstock seedlings does not appear to be cost-effective; however, other products should be evaluated.

scholarly journals Effek Konsentrasi Partikel Plastik terhadap Pertumbuhan dan Kelangsungan Hidup Ikan Bawal Bintang (Trachinotus blochii)

2020 ◽  
Vol 7 (1) ◽  
pp. 13
Author(s):  
Tengku Said Razai
Keyword(s):  
Survival Rates ◽  
Growth Yield ◽  
Aquatic Organisms ◽  
Household Waste ◽  
Control Treatment ◽  
Plastic Pollution ◽  
Growth And Survival ◽  
The Stability ◽  
Plastic Content ◽  
Trachinotus Blochii

Abstrac :Plastic pollution / waste has an impact that will disturb the stability of the ecosystem, especially aquatic organisms such as fish. The main source of plastic contamination comes from household waste that is not managed properly, especially in coastal areas that make the sea as a place of final discharge. Plastic pollution is currently the main problem that is accepted by the environment, especially water. Plastic will dissolve in the waters and will form small sizes (micro-plastic) impacting biota, including fish. Fish including aquatic organisms that can not distinguish between food and plastic particles clearly, thus providing an effect on digestion and growth. The results showed the growth rate of silver Pompanoo with the highest plastic concentration will cause slower growth, the control treatment is the best growth yield compared to treatments that have a plastic content mixed with feed. Growth of weights with the highest concentration of plastic particles is 0,48 gr/week, length growth is 0.75 cm / week, and survival is 69.17% and shows the growth and survival rates are worse or there is a tendency to worsen with an increase in the concentration of plastic contamination . Plastic particles consumed by fish are not digested properly and can even cause the growth response of fish to be slow even to death.

scholarly journals TINGKAT KELANGSUNGAN HIDUP BENIH IKAN BETOK (A.TESTUDINEUS, BLOCH) YANG DIPELIHARA DIDALAM WADAH YANG MENGGUNAKAN SHELTER BAMBU, PARALON DAN TEMPURUNG KELAPA

2020 ◽  
Vol 5 (2) ◽  
pp. 63
Author(s):  
M Yusuf Arifin ◽  
Muhammad Sugihartono ◽  
Agusten Roy Marpaung
Keyword(s):  
Research Design ◽  
Growth Rates ◽  
Survival Rates ◽  
Environmental Design ◽  
Control Treatment ◽  
Treatment Control ◽  
Growth And Survival ◽  
The People ◽  
Randomized Design ◽  
Completely Randomized Design

Betok fish is very popular with the people because of the delicious and tasty taste of the meat, therefore this type of fish is quite potential for cultivation. (Muslim 2008) in (Anggara et al 2013). Most people who cultivate betok fish (A.testudineus, Bloch) do not understand their character or behavior, so that many farmers experience complaints in maintenance, especially in their growth and survival. As an alternative to the maintenance of Betok fish (A. testudineus, Bloch), the growth rates and viability of the Betok (A. testudineus, Bloch) fry were reared in a container using a shellter. The research design used was an environmental design RAL (completely randomized design) consisting of 4 (four) treatments and 3 (three) replications. The treatments were as follows: Treatment A: Betok fish seeds (A. testudineus, Bloch) without treatment (control), Treatment B: Betok fish seeds (A. testudineus, Bloch) were given a bamboo shellter ,, Treatment C; Betok fish (A. testudineusBloch) was given a coconut shell shellter, Treatment D: Betok fish (A.testudineusBloch) was given a paralon shellter. The results showed that betok (A. tetstudineus, Bloch) seeds were maintained using a shelter (Treatment C and B) resulted in better survival rates (99.35% & 98.07%) than treatments A and D (96.15% & 96.79%).

scholarly journals Effect of Hormones (IBA & IAA) on the Propagation of Himalayan Yew in Pakistan: A Conservation Approach

2020 ◽  
Author(s):  
Javaid Iqbal ◽  
Bushra Khan ◽  
Sardar Khan ◽  
Nasreen Ghaffar ◽  
Ishaq Ahmad Mian ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Medicinal Plants ◽  
Butyric Acid ◽  
Indole Acetic Acid ◽  
Average Length ◽  
Stem Cuttings ◽  
Indole Butyric Acid ◽  
Number Of Leaves ◽  
Himalayan Yew ◽  
Taxus Wallichiana

Abstract Background Himalayan yew (Taxus wallichiana) is one of the endangered medicinal plants species having great importance due to the presence of anticancer drug Taxol. This metabolite is mainly used for the treatment of ovarian, breast, AIDS-related cancers, and other indications. The study being reported here was conducted for the propagation of Himalayan yew by using two different trials of Indole Butyric acid (IBA) and Indole acetic acid (IAA) hormones treatments (2000–7000 ppm) through stem cuttings (140 each). In the same way, 3840 cuttings were treated with IBA and IAA from November 2016 to November 2017 at Lalku valley, Swat, Khyber Pakhtunkhwa (KP), Pakistan. Results The influence of IBA treatment (7000 ppm) showed a survival of 85.22% (average number of roots = 10.4, average length of roots = 15.5 cm, average number of leaves = 92.4 and average number of sprouts = 3.3) while that of IAA treatment (7000 ppm) the survival of 81.11% (average number of roots = 9.1, average length of roots = 14.6 cm, average number of leaves = 84.0 and average number of sprout = 3.0) were more significant followed by 2000–6000 ppm (IBA and IAA). Lowest survival 40–45% (average number of roots = 4.2, average length of roots = 8.0 cm, average number of leaves = 32.2, average number of sprouts = 1.7) was noticed for controlled cuttings. Conclusion The present study enhanced the potential of conservation and propagation of T. wallichiana. Hence our study suggests and recommends the application of IBA (7000 ppm) as a better hormone for the conservation and propagation of Himalayan yew.

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