scholarly journals Antitranspirant Application Increases Grafting Success of Watermelon

2017 ◽  
Vol 27 (4) ◽  
pp. 494-501 ◽  
Author(s):  
Sahar Dabirian ◽  
Carol A. Miles
Keyword(s):  
Survival Rate ◽  
Stomatal Conductance ◽  
Citrullus Lanatus ◽  
Lagenaria Siceraria ◽  
Cucurbita Maxima ◽  
Water Application ◽  
Greenhouse Experiments ◽  
The One ◽  
Treatment Application ◽  
Grafting Methods

The one-cotyledon splice grafting method is commonly used for watermelon (Citrullus lanatus) because it is relatively rapid and there is less rootstock regrowth than with other grafting methods. However, plants must rely on moisture in the air for survival during at least the first 4 days after grafting. In 2015 and 2016, greenhouse experiments were conducted to investigate if application of commercial stomata-coating and stomata-closing antitranspirant products, applied 1 day before grafting to both scion and rootstock seedlings, could increase the survival of watermelon transplants grafted using the one-cotyledon method. ‘TriX Palomar’ watermelon was grafted onto rootstock ‘Tetsukabuto’ (Cucurbita maxima × C. moschata) in Expt. 1, and onto rootstock ‘Emphasis’ (Lagenaria siceraria) in Expt. 2. The survival of grafted watermelon differed because of experiment (P = 0.0003), antitranspirant treatment (P < 0.0001), and experimental repeat (P < 0.0001). The survival of ‘TriX Palomar’ grafted onto ‘Tetsukabuto’ was greatest for plants treated with the stomata-coating + stomata-closing antitranspirants (92% to 100%), followed by the stomata-closing antitranspirant (79% to 97%), water (72%), and the stomata-coating antitranspirant (50% to 60%). For ‘TriX Palomar’ grafted onto ‘Emphasis’, plants treated with the stomata-closing antitranspirant had the greatest survival (87% to 97%), followed by stomata-coating + stomata-closing antitranspirants (84% to 94%), the stomata-coating antitranspirant (50% to 67%), and water (53% to 68%). In Expt. 3, stomatal conductance (gS) was similar for both ‘TriX Palomar’ and ‘Emphasis’ seedlings before treatment application, but differed because of the treatments 1 and 2 days after application. Stomatal conductance did not change for ‘TriX Palomar’ seedlings after application of the stomata-coating antitranspirant or water, or for ‘Emphasis’ seedlings after application of the stomata-coating antitranspirant. Stomatal conductance of ‘TriX Palomar’ seedlings decreased 57% to 62% after application of the stomata-closing antitranspirant and decreased 48% to 60% after application of the stomata-coating + stomata-closing antitranspirants. Stomatal conductance for ‘Emphasis’ seedlings increased 37% after water application, and decreased 58% to 68% after application of the stomata-closing antitranspirant, and decreased 42% to 45% after application of the stomata-coating + stomata-closing antitranspirants. The survival rate of grafted ‘TriX Palomar’ transplants was increased nearly 30% by application 1 day before grafting of the commercial stomata-closing antitranspirant or stomata-coating + stomata-closing antitranspirants in this study. Increase in grafting success is likely due to a reduction in transpiration that occurs when the stomata-closing antitranspirant is applied to the seedlings before grafting.

scholarly journals Improvement of Grafted Watermelon Transplant Survival as a Result of Size and Starch Increases Over Time Caused by Rootstock Fatty Alcohol Treatment: Part II

2014 ◽  
Vol 24 (3) ◽  
pp. 350-354
Author(s):  
Shawna L. Daley ◽  
William Patrick Wechter ◽  
Richard L. Hassell
Keyword(s):  
Fatty Alcohol ◽  
Citrullus Lanatus ◽  
Soluble Sugar ◽  
Lagenaria Siceraria ◽  
Energy Reserves ◽  
Cucurbita Maxima ◽  
Stored Energy ◽  
Total Soluble Sugar ◽  
Alcohol Treatments ◽  
Over Time

Fatty alcohol treatments can be used to eliminate the meristem of cucurbit (Cucurbitaceae) rootstocks, which prevents regrowth when grafting, but the effects of the treatment on the rootstock have not been documented. Two rootstock types, ‘Emphasis’ bottle gourd (Lagenaria siceraria) and ‘Carnivor’ interspecific hybrid squash (Cucurbita maxima × C. moschata) commonly used in watermelon (Citrullus lanatus) grafting significantly increased in cotyledon and hypocotyl size over 21 days after treatment (DAT) with a 6.25% fatty alcohol emulsion. There was a significant increase in total soluble sugar (glucose, sucrose, and fructose) content for each rootstock hypocotyl and cotyledon. Starch concentrations of hypocotyls and cotyledons also increased significantly in both rootstocks. This increase in stored energy could greatly increase the success rate of the grafting process. Increased rootstock energy reserves could overcome the need for keeping the rootstock cotyledon intact when grafting.

scholarly journals Resistência de porta-enxertos de cucurbitáceas a nematóides e compatibilidade da enxertia em melão

2014 ◽  
Vol 32 (3) ◽  
pp. 297-302 ◽  
Author(s):  
Letícia A Ito ◽  
Lucas A Gaion ◽  
Francine S Galatti ◽  
Leila T Braz ◽  
Jaime M Santos
Keyword(s):  
Cucumis Sativus ◽  
Cucumis Melo ◽  
Citrullus Lanatus ◽  
Cucurbita Moschata ◽  
Lagenaria Siceraria ◽  
Cucurbita Maxima ◽  
Luffa Cylindrica ◽  
Benincasa Hispida

Devido ao severo ataque de nematoides na cultura do meloeiro rendilhado sob ambiente protegido, este trabalho teve por objetivo selecionar porta-enxertos resistentes a Meloidogyne incognitae M. javanica. O experimento foi conduzido em casa de vegetação, de outubro de 2010 a abril de 2011, em Jaboticabal-SP. Foram avaliados 33 porta-enxertos; melões: CNPH 01-930 (Cucumis melo var. flexuosus), CNPH 01-962, CNPH 01-963 (Cucumis melo var. conomon), cvs. Gaúcho Redondo, Gaúcho Comprido, Redondo Amarelo, Gulfcoast, Chilton, Bônus n° 2, Fantasy; melancias: cv. Charleston Gray, Progênie da Coréia (Citrullus lanatus); abóboras: cvs. Mra. Ma, Ornamental, Howden, Mammoth, Kururu, Goianinha (Cucurbita moschata); cabaça: Abóbora de Porco, cvs. Maranhão, Brasileirinha (Lagenaria siceraria); moranga: cv. Pataca Gigante (Cucurbita maxima); pepinos: cvs. Caipira, Branco Meio-Comprido, Curumim (Cucumis sativus); buchas: Metro, Semente Branca, Semente Preta (Luffa cylindrica); abóbora d'água (Benincasa hispida); abóbora porta-enxerto: Híbrido cv. Keij; quiabo de Metro (Trichosanthes cucumerins) e Cruá (Sicana odorifera). Para avaliar a resistência de plantas, as mudas foram transplantadas para vasos e inoculados com 3.000 ovos e juvenis de M. incognita via sistema radicular. Aos 50 dias após a inoculação, foi realizada avaliação da resistência das plantas, com base no fator de reprodução do nematoide. Foram realizados os mesmos procedimentos para as duas espécies de nematoides. Nos porta-enxertos resistentes aos nematoides foram realizadas enxertias, com os melões rendilhados 'Bônus n° 2' e 'Fantasy'. CNPH 01-962, CNPH 01-963 e melão 'Gaúcho Redondo' foram resistentes a M. incognita. Melão 'Redondo Amarelo', melancia 'Charleston Gray', Progênie da Coréia, e Trichosanthes cucumerins, foram resistentes a M. javanica. Benincasa hispida foi resistente a ambas as espécies. As compatibilidades de enxertia entre os porta-enxertos resistentes e os melões rendilhados 'Bônus no 2' e 'Fantasy' foram superiores a 98%.

scholarly journals Evaluating Cucurbit Rootstocks to Prevent Disease Caused by Pythium aphanidermatum and P. myriotylum on Watermelon

Plant Disease ◽  
2020 ◽  
Vol 104 (11) ◽  
pp. 3019-3025
Author(s):  
Sean M. Toporek ◽  
Anthony P. Keinath
Keyword(s):  
Interspecific Hybrid ◽  
Citrullus Lanatus ◽  
Disease Incidence ◽  
Bottle Gourd ◽  
Stem Rot ◽  
Lagenaria Siceraria ◽  
Cucurbita Maxima ◽  
Pythium Species ◽  
Seedless Watermelon ◽  
Marketable Fruit

Pythium species cause root and stem rot in watermelon (Citrullus lanatus), but cucurbit rootstocks used to graft watermelon have not been evaluated for resistance. P. aphanidermatum and P. myriotylum were inoculated onto 15 nongrafted watermelon, citron (Citrullus amarus), bottle gourd (Lagenaria siceraria), and interspecific hybrid squash (Cucurbita maxima × C. moschata) cultivars in a growth chamber. Watermelon was more susceptible than bottle gourd and interspecific hybrid squash at 20 and 30°C. Twenty-one cultivars were inoculated in a field with an equal blend of both Pythium species. Interspecific hybrid squash was less susceptible than bottle gourd and watermelon in 2018 and 2019. Seedless watermelon cultivar Tri-X 313 was grafted to one citron, one bottle gourd, and three interspecific hybrid squash rootstocks. Plants were inoculated in the field as described. Grafting to interspecific hybrid squash rootstocks reduced disease incidence compared with nongrafted controls in 2018 and 2019. Mefenoxam and propamocarb applied at transplanting did not affect disease compared with non-fungicide-treated plots. Grafting to interspecific hybrid squash Camelforce significantly increased total and marketable fruit numbers and total weight in 2019 compared with the nongrafted control. In summary, interspecific hybrid squash was consistently resistant to Pythium, demonstrating resistance and utility in watermelon grafting.

scholarly journals Contribuição das folhas cotiledonares para o crescimento e estabelecimento de plântulas de cucurbitáceas

2004 ◽  
Vol 22 (2) ◽  
pp. 309-313 ◽  
Author(s):  
Dilson Antônio Bisognin ◽  
Cassandro V.T. do Amarante ◽  
Jacso Dellai
Keyword(s):  
Cucumis Sativus ◽  
Citrullus Lanatus ◽  
Lagenaria Siceraria ◽  
Cucurbita Maxima

As cucurbitáceas apresentam germinação epigeal, cujos cotilédones têm a dupla função de fornecer substâncias de reserva e fotoassimilados para o crescimento e o estabelecimento da plântula. O objetivo deste trabalho foi quantificar o nível de dependência de plântulas de abóbora híbrida (Cucurbita maxima x C. moschata), melancia (Citrullus lanatus), pepino (Cucumis sativus) e porongo (Lagenaria siceraria) em relação às folhas cotiledonares para o crescimento e estabelecimento inicial. Foi efetuada a remoção de um ou dois cotilédones aos 3; 6; 9 e 12 dias após a emergência (DAE), comparando-se o crescimento das plântulas com o de plântulas controle (sem remoção). O crescimento inicial foi avaliado a cada três dias, da emergência até 21 DAE, determinando-se a taxa de expansão das folhas cotiledonares, o período de tempo até a equivalência entre área foliar e cotiledonar e a produção de matéria seca. A abóbora apresentou a maior taxa de expansão das folhas cotiledonares, o menor período até a emissão e expansão das folhas verdadeiras e a maior produção de matéria seca. As plântulas de abóbora e pepino apresentaram a maior dependência em relação às folhas cotiledonares. Os resultados obtidos mostram que danos às folhas cotiledonares, dependendo da espécie, da intensidade e da idade da plântula, podem afetar drasticamente o crescimento inicial e o estabelecimento das plântulas, com reflexos evidentes no rendimento de frutos em cucurbitáceas.

scholarly journals Reproduction of Didymella bryoniae on Nine Species of Cucurbits Under Field Conditions

Plant Disease ◽  
2014 ◽  
Vol 98 (10) ◽  
pp. 1379-1386 ◽  
Author(s):  
Anthony P. Keinath
Keyword(s):  
Environmental Conditions ◽  
Cucumis Melo ◽  
Citrullus Lanatus ◽  
Lagenaria Siceraria ◽  
Cucurbita Maxima ◽  
Fruiting Bodies ◽  
Didymella Bryoniae ◽  
Plant Parts ◽  
Gummy Stem Blight ◽  
Field Plots

Eighteen cucurbit cultivars representing 5 genera, 9 species, and 11 taxa susceptible to gummy stem blight were inoculated with Didymella bryoniae in field plots in Charleston, SC, in autumn 2008, autumn 2009, and spring 2011 to determine the suitability of the hosts and various plant parts for formation of sexual and asexual fruiting bodies of the pathogen. In 1, 2, or 3 years, D. bryoniae reproduced on all 18 cultivars, on leaves, and on all plant parts examined—pedicles, peduncles, petioles, tendrils, and vines. Watermelon and citron (both Citrullus lanatus) and melon (Cucumis melo) had significantly more leaves with fruiting bodies than cucumber (Cucumis sativus), bottle gourd (Lagenaria siceraria), and eight cultivars of squash and pumpkin (Cucurbita maxima, C. moschata, and C. pepo). On plant parts other than leaves, melon had the greatest proportions of tissue pieces with fruiting bodies, and citron had the least. Fruiting bodies were observed on 86% of plant parts examined in autumn 2009 but on only 28% in spring 2011, when environmental conditions were hot and dry. In 2009, pseudothecia and pycnidia were found in equal proportions on leaves, but pseudothecia were found more frequently than pycnidia on leaves in 2011 and on other plant parts in 2009 and 2011.

scholarly journals RESULTS OF INTRODUCTION OF MOMORDICA CHARANTIA L. WHEN GRAFTING ON PUMPKIN VARIETIES IN THE CONDITIONS OF THE MIDDLE CIS-URAL REGION

2021 ◽  
Vol 212 ◽  
pp. 112-116
Author(s):  
S. A. Musikhin ◽  
◽  
A. V. Fedorov ◽  
O. A. Ardasheva ◽  
◽  
...  
Keyword(s):  
Survival Rate ◽  
Dry Matter ◽  
Humus Content ◽  
Sugar Content ◽  
Total Sugar ◽  
Momordica Charantia ◽  
Middle Urals ◽  
High Survival ◽  
Lagenaria Siceraria ◽  
Cucurbita Maxima

In the context of tremendous changes in the world community, one of the main factors is introduction of new plant varieties for reliable development of agriculture. To improve the quality of adaptive capabilities of thermophilic pumpkin crops in the Middle Urals, grafting on resistant stocks can be used. The studies were carried out in 2018-2020 on protected ground, at Udmurt Federal Research Center of the Ural Branch of the Russian Academy of Sciences, the city of Izhevsk. The soil is organo-mineral, humus content is 5.0 %, pH - 5.8. The total plot area is 24 m2, the record area is 16 m2. The repetition was fourfold, the placement of the plots was randomized. The experiment is one-factor. Experimental variants are: 1. Momordica charantia L. without grafting (control), stocks - 2. Cucurbita pepo L., 3. Cucurbita maxima Duch., 4. Cucurbita moschata Duch., 5. Cucurbita ficifolia Bouche., 6. Lagenaria siceraria Molina , Standl. This work presents the stages of the study on compatibility of Momordica charantia when it was grafted onto various types of stocks, based on the analysis of the characteristics of growth and development, survival and productivity of plants. Conclusions were made about the prospects of growing Momordica charantia indoors. The data obtained indicate a high survival rate of Momordica charantia - over 72 % of the studied stocks. At the same time, it should be noted that the highest survival rate among grafts had Cucurbita maxima variant. The dry matter and total sugar content in the fruits of Momordica charantia increased in all variants with grafting (to 26.8 % by dry matter and to 16.2 % by total sugar, respectively) compared to the control, and the accumulation of nitrates was maximum - 384.6 mg / kg in the variant of grafting on Lagenaria siceraria, while there was no excess of MAC.

scholarly journals A New Grafting Method for Watermelon to Inhibit Rootstock Regrowth and Enhance Scion Growth

Agriculture ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 812
Author(s):  
Changjin Liu ◽  
Weiguo Lin ◽  
Chongran Feng ◽  
Xiangshuai Wu ◽  
Xiaohu Fu ◽  
...  
Keyword(s):  
Citrullus Lanatus ◽  
Bottle Gourd ◽  
New Method ◽  
Plant Management ◽  
Lagenaria Siceraria ◽  
Manual Removal ◽  
Labor Inputs ◽  
Time Required ◽  
Grafting Methods

Grafting is a widely used technique in watermelon (Citrullus lanatus) production. However, cost of grafted seedlings is generally high as a result of intensive labor inputs for propagation using traditional grafting methods such as the manual removal of rootstock regrowth. This study developed a new grafting tool to physically remove the epidermis of pumpkin (C. maxima × C. moschata) and bottle gourd (Lagenaria siceraria) rootstock cotyledon base during grafting; we called this a new grafting method. Compared with the traditional grafting, the new grafting method significantly decreased the pumpkin rootstock regrowth rate from 100% to 8% in hole insertion and 2% in one cotyledon grafting, respectively. These attenuated rates for bottle gourd rootstock regrowth were 23% and 9% in hole insertion and one cotyledon grafting, respectively. The scion dry weights of new hole insertion and one cotyledon grafting were increased by 78% and 74% when pumpkin was used as rootstock as compared with traditional grafting without regrown rootstock removal, while the respective values were 33% and 17% in bottle gourd rootstock grafted plants. In addition, the time used for the new hole insertion grafting method to physically remove the epidermis of pumpkin rootstock cotyledon base was significantly shorter than the time required to remove the rootstock regrowth manually three times in the traditional grafting (4.2 s/plant vs. 9.3 s/plant). Similar results were also observed in the new one cotyledon grafting (4.2 s/plant vs. 8.8 s/plant). Taken together, this study presents a new method in watermelon grafting to reduce rootstock regrowth, therefore benefiting both scion growth and plant management, thus the development of this new method is clearly useful in watermelon production.

scholarly journals Efficient Cryopreservation of Populus tremula by In Vitro-Grown Axillary Buds and Genetic Stability of Recovered Plants

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 77
Author(s):  
Elena O. Vidyagina ◽  
Nikolay N. Kharchenko ◽  
Konstantin A. Shestibratov
Keyword(s):  
Survival Rate ◽  
Axillary Buds ◽  
Long Term Storage ◽  
Average Survival ◽  
Transgenic Lines ◽  
Ssr Loci ◽  
One Step ◽  
The One

Axillary buds of in vitro microshoots were successfully frozen at –196 °C by the one-step freezing method using the protective vitrification solution 2 (PVS2). Microshoots were taken from 11 transgenic lines and three wild type lines. Influence of different explant pretreatments were analyzed from the point of their influence towards recovery after cryopreservation. It was found out that the use of axillary buds as explants after removal of the apical one increases recovery on average by 8%. The cultivation on growth medium of higher density insignificantly raises the regenerants survival rate. Pretreatment of the osmotic fluid (OF) shows the greatest influence on the survival rate. It leads to the increase in survival rate by 20%. The cryopreservation technology providing regenerants average survival rate of 83% was developed. It was based on the experimental results obtained with explant pretreatment. Incubation time in liquid nitrogen did not affect the explants survival rate after thawing. After six months cryostorage of samples their genetic variability was analyzed. Six variable simple sequence repeat (SSR) loci were used to analyze genotype variability after the freezing-thawing procedure. The microsatellite analysis showed the genetic status identity of plants after cryopreservation and of the original genotypes. The presence of the recombinant gene in the transgenic lines after cryostorage were confirmed so as the interclonal variation in the growth rate under greenhouse conditions. The developed technique is recommended for long-term storage of various breeding and genetically modified lines of aspen plants, as it provides a high percentage of explants survival with no changes in genotype.

scholarly journals Enzymes of the one-carbon folate metabolism as anticancer targets predicted by survival rate analysis

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Jun Koseki ◽  
Masamitsu Konno ◽  
Ayumu Asai ◽  
Hugh Colvin ◽  
Koichi Kawamoto ◽  
...  
Keyword(s):  
Survival Rate ◽  
Folate Metabolism ◽  
Rate Analysis ◽  
The One

scholarly journals INFLUENCE OF ROOTSTOCKS ON Fusarium WILT, NEMATODE INFESTATION, YIELD AND FRUIT QUALITY IN WATERMELON PRODUCTION

2015 ◽  
Vol 39 (4) ◽  
pp. 323-330 ◽  
Author(s):  
Juan Carlos Álvarez-Hernández ◽  
Javier Zaragoza Castellanos-Ramos ◽  
César Leobardo Aguirre-Mancilla ◽  
María Victoria Huitrón-Ramírez ◽  
Francisco Camacho-Ferre
Keyword(s):  
Fusarium Oxysporum ◽  
Fruit Quality ◽  
Meloidogyne Incognita ◽  
Citrullus Lanatus ◽  
The Other ◽  
Cucurbita Moschata ◽  
Cucurbita Maxima ◽  
Plant Densities ◽  
Production Cycles ◽  
Lower Plant

Cucurbita maxima x Cucurbita moschata rootstock are used to prevent infection with Fusarium oxysporum f. sp. niveum in watermelon production; however, this rootstock is not effective against nematode attack. Because of their vigor, the grafted plants can be planted at lower plant densities than the non-grafted plants. The tolerance to Fusarium oxysporum f. sp. niveum and Meloidogyne incognita was assessed in watermelon plants grafted onto a hybrid of Citrullus lanatus cv Robusta or the Cucurbita maxima x Cucurbita moschata cv Super Shintoza rootstocks. The densities of plants were 2083 and 4166 plants ha-1. Non-grafted watermelons were the controls. The Crunchy Red and Sangría watermelon cultivars were used as the scions, it the latter as a pollinator. The experiments were performed for two production cycles in soils infested with Fusarium oxysporum f. sp. niveum and Meloidogyne incognita. The incidence of Fusarium oxysporum f. sp. niveum was significantly greater in the non-grafted than in the grafted plants. The grafted plants presented similar resistance to Fusarium regardless of the rootstock. The root-knot galling index for Meloidogyne incognita was significantly lower in plants grafted onto Citrullus lanatus cv Robusta than onto the other rootstock. The yields of plants grafted onto Citrullus lanatus cv Robusta grown at both plant densities were significantly higher than in the other treatments.

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