Genetic Analyses of Soluble Carbohydrate Concentrations in Onion Bulbs

Fructans are the main soluble carbohydrate in onion (Allium cepa) bulbs and their concentrations show significant correlations with dry weights and pungency. In previous research, we identified regions on chromosomes 5 and 8 associated with higher amounts of soluble carbohydrates in onion bulbs. In this research, we estimated the genetic effects and interactions between these two chromosome regions using larger inbred families grown in field trials over 3 years. Bulbs were evaluated 30 and 90 days after harvest (DAH) for dry weights and soluble carbohydrates. Fructan concentrations decreased significantly between 30 and 90 DAH, consistent with loss of bulb dormancy over this period. Dry weights were negatively correlated with fructose and positively correlated with sucrose and fructans. Analyses of variance and interval mapping revealed that the region on chromosome 5 affected bulb dry weight, whereas the region on chromosome 8 significantly affected both dry weight and fructan concentrations. Regions on chromosomes 5 and 8 showed dominance for increased dry weights and/or soluble solids. Interactions between regions on chromosomes 5 and 8 were significant only for sucrose and the fructan neokestose, indicating that these regions independently contribute to higher amounts of soluble carbohydrates. These results demonstrate that onions with low concentrations of soluble carbohydrates were developed by selecting for relatively few recessively inherited chromosome regions.

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QTL affecting soluble carbohydrate concentrations in stored onion bulbs and their association with flavor and health-enhancing attributes

Genome ◽

10.1139/g04-005 ◽

2004 ◽

Vol 47 (3) ◽

pp. 463-468 ◽

Cited By ~ 29

Author(s):

Michael J Havey ◽

Claudio R Galmarini ◽

Ali Fuat Gökçe ◽

Cynthia Henson

Keyword(s):

Dry Weight ◽

Interval Mapping ◽

Field Trials ◽

Soluble Carbohydrates ◽

Soluble Carbohydrate ◽

Onion Bulbs ◽

Invertase Gene ◽

Trait Locus ◽

Genomic Regions ◽

Group D

Onion bulbs accumulate fructans, a type of soluble carbohydrate associated with lower rates of colorectal cancers. Higher fructan concentrations in bulbs are correlated with higher pungency, longer dormancy, and greater onion-induced antiplatelet activity (OIAA). We analyzed replicated field trials of a segregating family for types and concentrations of soluble carbohydrates in onion bulbs 90 days after harvest. Means were adjusted using dry weight as the covariant to reveal highly significant (P < 0.001) differences among parents and families for glucose, fructose, sucrose, and the fructans 1-kestose, neokestose, and (6G,1)-nystose. Fructan concentrations showed significant (P < 0.05) phenotypic correlations with each other and with sucrose, pungency, and OIAA. These observations are consistent with the hypothesis that onion bulbs accumulating fructans take up or retain less water, concentrating both soluble carbohydrates and thiosulfinates responsible for pungency and OIAA. Interval mapping of family means from the covariant analyses revealed regions on linkage groups A and D significantly (LOD > 2.68) affecting soluble carbohydrate concentrations. The enzyme catalyzing the first step of fructan polymerization, 1-sucrose-sucrose fructosyltransferase (1-SST), mapped independently of these genomic regions. One region on linkage group D near an acid-invertase gene was significantly (LOD = 3.45) associated with sucrose concentrations. This study reveals that the accumulation of sucrose in stored onion bulbs may allow for the combination of sweeter flavor with significant OIAA.Key words: quantitative trait locus, fructans, thiosulfinates, reducing sugars.

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Arsenic Application Changed Growth, Photo-Synthetic Pigments and Antioxidant Enzymes Activity in Sorghum (Sorghum bicolor L.) Under Salinity Stress

Scientia Agriculturae Bohemica ◽

10.2478/sab-2019-0021 ◽

2019 ◽

Vol 50 (3) ◽

pp. 155-163 ◽

Cited By ~ 1

Author(s):

B. Talebi ◽

M. Heidari ◽

H. Ghorbani

Keyword(s):

Plant Biomass ◽

Dry Weight ◽

Carotenoid Content ◽

Soluble Carbohydrates ◽

Soluble Carbohydrate ◽

Guaiacol Peroxidase ◽

Anthocyanin Content ◽

Synthetic Pigments ◽

Root Tissues ◽

Gpx Activity

Abstract The elevation of arsenic (As) content in soils is of considerable concern with respect to its uptake by plant and subsequent entry into wildlife and human food chains. The treatment of sorghum seedlings with As as NaH2As4O. 7H2O at various concentrations (A1 = 0, A2 = 20, A3 = 40 and A4 = 60 mg As kg−1 soil) and salinity at four different levels (S1 = 0, S2 = 3, S3 = 6 and S3 = 9 dS m−1) reduced fresh and dry weights of sorghum plants. The co-application of As and salinity increased the guaiacol peroxidase (GPX) activity in shoot and root tissues. The highest GPX activity in shoot and root tissues was obtained at S2A4 and S3A3 treatments, respectively. The activity of catalase (CAT) in shoot was not changed, but unlike the GPX activity, salinity and As decreased the CAT activity in root tissues. Concerning the photosynthesis pigments, salinity had no effect on the chlorophyll ‘a’, chlorophyll ‘b’ and carotenoid content in leaves, but the As treatment significantly decreased the content of both chlorophyll types. Salinity increased the anthocyanin content in leaves. There were negative correlation between soluble carbohydrates (r2 = −0.78**) and stomata conductance (r2 = −0.45**) and dry weight of the plant biomass in this study. By increasing the salinity and As concentration in root medium, soluble carbohydrate in leaves increased but salinity decreased the leaf stomata conductance.

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Genotypic variation in water-soluble carbohydrate accumulation in wheat

Functional Plant Biology ◽

10.1071/fp06062 ◽

2006 ◽

Vol 33 (9) ◽

pp. 799 ◽

Cited By ~ 125

Author(s):

Sari A. Ruuska ◽

Greg J. Rebetzke ◽

Anthony F. van Herwaarden ◽

Richard A. Richards ◽

Neil A. Fettell ◽

...

Keyword(s):

Genotypic Variation ◽

Dry Weight ◽

Grain Filling ◽

Water Soluble ◽

Soluble Carbohydrates ◽

Soluble Carbohydrate ◽

Broad Sense Heritability ◽

Carbohydrate Accumulation ◽

Sequential Mechanism ◽

Water Soluble Carbohydrate

The water-soluble carbohydrate (WSC) that accumulates in the stems of wheat during growth can be an important contributor to grain filling, particularly under conditions when assimilation is limited, such as during end-of-season drought. WSC concentration was measured at anthesis across a diverse set of wheat genotypes over multiple environments. Environmental differences in WSC concentration were large (means for the set ranging between 108 and 203 mg g–1 dry weight), and there were significant and repeatable differences in WSC accumulation among genotypes (means ranging from 112 to 213 mg g–1 dry weight averaged across environments), associated with large broad-sense heritability (H = 0.90 ± 0.12). These results suggest that breeding for high WSC should be possible in wheat. The composition of the WSC, examined in selected genotypes, indicated that the variation in total WSC was attributed mainly to variation in the fructan component, with the other major soluble carbohydrates, sucrose and hexose, varying less. The degree of polymerisation (DP) of fructo-oligosaccharides was up to ~13 in samples where higher levels of WSC were accumulated, owing either to genotype or environment, but the higher DP components (DP > 6) were decreased in samples of lower total WSC. The results are consistent with fructan biosynthesis occurring via a sequential mechanism that is dependent on the availability of sucrose, and differences in WSC contents of genotypes are unlikely to be due to major mechanistic differences.

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(295) Relationship Between Macromolecule and Metal Ion Accumulation in Two Salt-stressed Cultivars of Sweetpotato under In Vitro Conditions

HortScience ◽

10.21273/hortsci.41.4.1079a ◽

2006 ◽

Vol 41 (4) ◽

pp. 1079A-1079

Author(s):

Devi Prasad V. Potluri

Keyword(s):

Salt Stress ◽

Metal Ion ◽

Ipomoea Batatas ◽

Dry Weight ◽

Soluble Carbohydrates ◽

Soluble Carbohydrate ◽

Shoot Height ◽

Sodium Accumulation ◽

Carbohydrate Levels

Two cultivars of sweetpotato [Ipomoea batatas (L.) Lam.], Commensal and Salyboro, were subjected to salt stress using axillary bud cultures. The salt levels ranged from 0–150 mM. After 10 weeks of growth, plantlet shoot height, dry weight, number of nodes, levels of proline, soluble carbohydrate, and protein; and metal ions sodium, potassium, magnesium, and calcium, were measured. In both cultivars, proline accumulation was higher in the shoot. There was a positive correlation between the increase in soluble carbohydrates and proteins in `Commnesal', but not in `Salyboro'. More sodium accumulated in the shoots of `Salyboro' compared to `Commensal'. The accumulation of sodium reduced the calcium and potassium, but not magnesium levels. Increase in sodium levels correlated with the increase in soluble carbohydrate levels is `Salyboro', but not in `Commensal'. A similar trend was evident with praline and sodium accumulation. Based on these and previous results, the cultivar `Salyboro' appears to be more susceptible to salt stress.

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Sulfur Nutrition Affects Cellular Sulfur, Dry Weight Distribution, and Bulb Quality in Onion

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.126.2.164 ◽

2001 ◽

Vol 126 (2) ◽

pp. 164-168 ◽

Cited By ~ 12

Author(s):

Jane E. Lancaster ◽

Julie Farrant ◽

Martin L. Shaw

Keyword(s):

Cell Wall ◽

Soluble Sugars ◽

Dry Weight ◽

Major Effect ◽

Soluble Solids ◽

Minor Effect ◽

Cell Wall Proteins ◽

Onion Bulbs ◽

Solids Concentration ◽

Cell Fractions

Three onion (Allium cepa L.) cultivars, `Southport White Globe', `Grano', and `Pukekohe Longkeeper' were grown at low to high S (at 0.5, 1.8, 3.0 or 4.0 meq·L-1) in hydroponic culture. Differential solvent extractions of bulbs were used to isolate quantitatively cell contents, cell wall proteins, and cell wall residue. The weight of the cell fractions, their S content, and the S content of intact bulbs were determined. Bulb characteristics of fresh weight (FW), firmness, soluble solids concentration (SSC), and soluble sugars were also determined. For all three cultivars, bulb FW increased with S from 0.5 to 4.0 meq·L-1. Sulfur had a significant effect on bulb firmness. Onion bulbs grown with S at 0.5 meq·L-1, the lowest S concentration, were significantly softer than onion bulbs grown at the highest concentration of 4.0 meq·L-1. Varying the S supply had a major effect on dry weight (DW) allocation to the cell wall residue. Bulbs of all three cultivars grown at the lowest S had significantly less DW in the cell walls compared to S at 3.0 or 4.0 meq·L-1. In contrast to the effect of S supply on DW allocation, varying S supply had no effect on total bulb S, free SO4-2, and on the S content of the cell contents and the cell wall residue and only a minor effect on cell wall proteins. There was no significant effect of S supply on either SSC or soluble sugars. At low S nutrition, which is limiting to the growth of onion bulbs, cell wall deposition is reduced, with a consequent decrease in bulb firmness. The S composition of the cellular components is maintained at the expense of bulb growth.

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Vermicompost Effects on Bulbing Dynamics, Nonstructural Carbohydrate Content, Yield, and Quality of `Rosado Paraguayo' Garlic Bulbs

HortScience ◽

10.21273/hortsci.41.3.589 ◽

2006 ◽

Vol 41 (3) ◽

pp. 589-592 ◽

Cited By ~ 18

Author(s):

Juan A. Argüello ◽

Alicia Ledesma ◽

Selva B. Núñez ◽

Carlos H. Rodríguez ◽

María del C. Díaz Goldfarb

Keyword(s):

Dry Weight ◽

Distribution Patterns ◽

Soluble Solids ◽

Soluble Carbohydrates ◽

Yield And Quality ◽

Nonstructural Carbohydrate ◽

Garlic Bulb ◽

Solids Content ◽

The Impact

The objectives of this work were to a) determine vermicompost effect on bulbification dynamics in terms of garlic (Allium sativum L.) bulb dry weight and sucrose metabolism and b) evaluate the impact of vermicompost on garlic bulb yield and quality. The treatments were soil (control) and 1 soil: 1 vermicompost (by volume). The use of vermicompost as a substrate caused early bulbing (18 to 20 days) and lengthened bulb filling period. Bulb filling period corresponded to an increase in the total soluble carbohydrates and a later modification in nonstructural carbohydrate distribution patterns regarding fructan (scorodose) metabolism. The vermicompost treatment increased scorodose accumulation, which was directly related to the harvest index, resulting in greater yield and bulb quality. Bulb quality was not modified in terms of bulb pungency and soluble solids content by the use of vermicompost.

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Cyclitol galactosides in embryos of buckwheat stem–leaf–seed explants fed D-chiro-inositol, myo-inositol or D-pinitol

Seed Science Research ◽

10.1079/ssr2005221 ◽

2005 ◽

Vol 15 (4) ◽

pp. 329-338 ◽

Cited By ~ 15

Author(s):

Janet M. Ma ◽

Marcin Horbowicz ◽

Ralph L. Obendorf

Keyword(s):

Effective Means ◽

Dry Weight ◽

Insulin Dependent Diabetes Mellitus ◽

Control Treatment ◽

Soluble Carbohydrates ◽

Dependent Diabetes Mellitus ◽

Soluble Carbohydrate ◽

Mature Embryos ◽

Legume Seeds ◽

Stem Leaf

Crop seeds accumulate soluble carbohydrates as part of their maturation process. In legume seeds, the major soluble carbohydrates are sucrose and its galactosides raffinose, stachyose and verbascose. In buckwheat (Fagopyrum esculentum Moench) seeds, the major soluble carbohydrates are sucrose and galactosides of d-chiro-inositol, named fagopyritols. This study was conducted to determine changes in soluble carbohydrate accumulation in embryos of buckwheat seeds after feeding solutions containing the free cyclitols D-chiro-inositol, myo-inositol and d-pinitol to stem–leaf–seed explants. Feeding D-chiro-inositol to explants resulted in a fourfold to fivefold increase in the accumulation of free d-chiro-inositol, fagopyritol A1 and fagopyritol B1 in embryos of mature seeds, but resulted in 30% less embryo dry weight compared to the control treatment without cyclitols. Feeding myo-inositol to buckwheat explants increased d-chiro-inositol in leaves and increased accumulation of fagopyritol A1 and fagopyritol B1 fivefold in embryos, fagopyritol A2 and fagopyritol B2 fourfold; fagopyritol A3 and fagopyritol B3 were also detected, with no reduction in accumulated embryo dry weight. Feeding d-pinitol to buckwheat explants resulted in accumulation of free d-pinitol in mature embryos, but not galactopinitols. D-Pinitol, galactopinitol A and galactopinitol B were not detected in embryos from explants fed solutions without d-pinitol. Feeding d-pinitol also resulted in reduced D-chiro-inositol accumulation by buckwheat seeds. The results indicate that myo-inositol may be the precursor to d-chiro-inositol synthesis, and fagopyritols accumulated in response to d-chiro-inositol availability in the embryo. We suggest that increasing myo-inositol in buckwheat maternal tissues may be an effective means to enhance the accumulation of D-chiro-inositol and fagopyritols in seeds, compounds that may be beneficial for the treatment of non-insulin-dependent diabetes mellitus.

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CRISPR/Cas9-Mediated Knockout of Galactinol Synthase-Encoding Genes Reduces Raffinose Family Oligosaccharide Levels in Soybean Seeds

Frontiers in Plant Science ◽

10.3389/fpls.2020.612942 ◽

2020 ◽

Vol 11 ◽

Author(s):

Huy Le ◽

Nhung Hong Nguyen ◽

Dong Thị Ta ◽

Thao Nhu Thi Le ◽

Thao Phuong Bui ◽

...

Keyword(s):

Double Mutant ◽

Crop Improvement ◽

Soybean Seed ◽

Dry Weight ◽

Soluble Carbohydrates ◽

Soluble Carbohydrate ◽

Soybean Seeds ◽

Galactinol Synthase ◽

Target Sites ◽

Mutant Lines

Raffinose family oligosaccharides (RFOs) are major soluble carbohydrates in soybean seeds that cannot be digested by human and other monogastric animals. Hence, a major goal is to reduce RFO levels to improve the nutritional quality of soybean. In this study, we utilized a dual gRNAs CRISPR/Cas9 system to induce knockouts in two soybean galactinol synthase (GOLS) genes, GmGOLS1A and its homeolog GmGOLS1B. Genotyping of T0 plants showed that the construct design was efficient in inducing various deletions in the target sites or sequences spanning the two target sites of both GmGOLS1A and GmGOLS1B genes. A subset of induced alleles was successfully transferred to progeny and, at the T2 generation, we identified null segregants of single and double mutant genotypes without off-target induced mutations. The seed carbohydrate analysis of double mutant lines showed a reduction in the total RFO content of soybean seed from 64.7 mg/g dry weight to 41.95 mg/g dry weight, a 35.2% decrease. On average, the stachyose content, the most predominant RFO in soybean seeds, decreased by 35.4% in double mutant soybean, while the raffinose content increased by 41.7%. A slight decrease in verbascose content was also observed in mutant lines. Aside from changes in soluble carbohydrate content, some mutant lines also exhibited increased protein and fat contents. Otherwise, no difference in seed weight, seed germination, plant development and morphology was observed in the mutants. Our findings indicate that GmGOLS1A and GmGOLS1B contribute to the soybean oligosaccharide profile through RFO biosynthesis pathways, and are promising targets for future investigation, as well as crop improvement efforts. Our results also demonstrate the potential in using elite soybean cultivars for transformation and targeted genome editing.

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Herbicidal potential of Aerva javanica to control wild oat in wheat crop

Allelopathy Journal ◽

10.26651/allelo.j/2020-51-2-1299 ◽

2020 ◽

Vol 51 (2) ◽

pp. 177-194

Author(s):

Al-Ghamdi A.M. ◽

El-Zohri M

Keyword(s):

Dry Weight ◽

Soluble Carbohydrates ◽

Wheat Crop ◽

Aqueous Extracts ◽

Wild Oat ◽

Culture Experiment ◽

Allelopathic Potential ◽

Root Extracts ◽

Wheat Germination ◽

Radical Length

We investigated the phytotoxicity of desert cotton (Aerva javanica) extracts on wild oat and wheat. Aqueous extracts from A. javanica roots, leaves and inflorescences collected from Jeddah and Al-Baha regions, Saudi Arabia were used. Generally, the allelopathic potential of water extracts of A. javanica collected from Jeddah were more in inhibitory to wild oat germination and seedlings growth than those from Al-Baha. In both regions, root extracts were inhibitory to wild oat followed by leaves and inflorescences extracts. All test aqueous extracts of both regions did not inhibit the wheat germination or seedlings growth.Whreas, the wild oat germination was reduced by root extracts 58.62 %, 28.62 % leaves extracts : 32.72 %, 17.72 % and inflorescences extract 28.11 %, 12.13 % by in plants samples collected from Jeddah and Al-Baha, respectively. Wild oat radical length was inhibited by root extracts 53.27 %, 32.84 % leaves 42.35 %, 9.63 % and inflorescences extracts 22.64 %, 16.75 % in case of Jeddah and Al-Baha plants, respectively. In pot culture experiment, all treatments markedly reduced the plant dry weight and soluble carbohydrates, proteins and free amino acids contents in wild oat. The differences in the allelopathic potentials of studied A. javanica extracts were related to the qualitative variations in their phytochemicals constituents. Our results showed that A. javanica extracts could be safely used to control wild oat growth in wheat fields after more detsaled research..

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Comparative Effect of Groundnut (Arachis hypogea l.) Genotypes on Yield and N Fixation in Sudan and Northern Guinea Savannahs of Nigeria

10.36265/njss.2019.290208 ◽

2019 ◽

pp. 61-67

Keyword(s):

Agricultural Research ◽

Cropping Systems ◽

Block Design ◽

Dry Weight ◽

Field Trials ◽

Yield Potential ◽

N Fixation ◽

Maize Crop ◽

Significant Interaction Effect ◽

Pod Yield

Recognition of high yielding and nitrogen (N) fixing groundnut genotypes and desegregating them in the cereal-based cropping systems common in savannah regions will enhance food security and reduce the need for high N fertilizers hence, minimize the high cost and associated environmental consequences. Field trials were conducted during the 2015 growing season at the Research Farms of Bayero University Kano (BUK) and Institute for Agricultural Research (IAR), Ahmadu Bello University, Samaru-Zaria to assess the yield potential and Biolog- ical N fixation in 15 groundnut genotypes (ICG 4729, ICGV-IS 07823, ICGV-IS 07893, ICGV-IS 07908, ICGV- SM 07539, ICGV- SM 07599, ICGV-IS 09926, ICGV-IS 09932, ICGV-IS 09992, ICGV-IS 09994, SAMNUT-21, SAMNUT-22, SAMNUT-25, KAMPALA and KWANKWAS). The groundnut genotypes and reference Maize crop (SAMMAZ 29) were planted in a randomized complete block design in three replications. N difference method was used to estimate the amount of N fixed. The parameters determined were the number of nodules, nod- ule dry weight, shoot and root dry weights, pod, and haulm yield as well as N fixation. The nodule dry weight, BNF, haulm, and pod yield were statistically significant (P<0.01) concerning genotype and location. Similarly, their interac- tion effect was also highly significant. ICGV-IS 09926 recorded the highest nod- ule dry weight of 2.07mg /plant across the locations while ICGV-IS 09932 had the highest BNF value of 140.27Kg/ha. Additionally, KAMPALA had the high- est haulm yield, while ICGV-IS 07893 had the highest pod yield across the loca- tions with a significant interaction effect. The result shows that ICGV-IS 07893 and ICGV-IS 09932, as well as ICGV-IS 09994 and SAMNUT – 22, were the best genotypes concerning BNF, haulm and pod yield in the Northern Guinea and Sudan Savannahs of Nigeria respectively with the potential for a corresponding beneficial effect.

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