scholarly journals Metabolic Responses of Hybrid Bermudagrass to Short-term and Long-term Drought Stress

2012 ◽  
Vol 137 (6) ◽  
pp. 411-420 ◽  
Author(s):  
Hongmei Du ◽  
Zhaolong Wang ◽  
Wenjuan Yu ◽  
Bingru Huang
Keyword(s):  
Drought Stress ◽  
Malic Acid ◽  
Photochemical Efficiency ◽  
Nitrogen Compound ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Grass Species ◽  
Severe Drought ◽  
Short Term

The accumulation of different types of metabolites may reflect variations in plant adaptation to different severities or durations of drought stress. The objectives of this project are to examine changes in metabolomic profiles and determine predominant metabolites in response to short-term (6 days) and long-term (18 days) drought stress with gas chromatography–mass spectrometry analysis in a C4 perennial grass species. Plants of hybrid bermudagrass (Cynodon dactylon × C. transvaalensis cv. Tifdwarf) were unirrigated for 18 days to induce drought stress in growth chambers. Physiological responses to drought stress were evaluated by visual rating of grass quality, relative water content, photochemical efficiency, and electrolyte leakage (EL). All parameters decreased significantly at 6 and 18 days of drought stress, except EL, which increased with the duration of drought stress. Under short-term drought stress (6 days), the content did not change significantly for most metabolites, except methionine, serine, γ-aminobutyric acid (GABA), isoleucine, and mannose. Most metabolites showed higher accumulation under long-term drought stress compared with that under the well-watered conditions, including three organic acids (malic acid, galacturonic acid, and succinic acid), 10 amino acids (proline, asparagine, phenylalanine, methionine, serine, 5-hydroxynorvaline, GABA, glycine, theorine, valine), seven sugars (sucrose, glucose, galactose, fructose, mannose, maltose, xylose), one nitrogen compound (ethanolamine), and two-sugar alcohol (myo-inositol). The accumulation of those metabolites, especially malic acid, proline, and sucrose, could be associated with drought adaptation of C4 hybrid bermudagrass to long-term or severe drought stress.

scholarly journals Phyllosphere community assembly and response to drought stress on common tropical and temperate forage grasses

2021 ◽  
Author(s):  
Emily K. Bechtold ◽  
Stephanie Ryan ◽  
Sarah E. Moughan ◽  
Ravi Ranjan ◽  
Klaus Nüsslein
Keyword(s):  
Microbial Community ◽  
Drought Stress ◽  
Bacterial Community ◽  
Community Assembly ◽  
Community Diversity ◽  
Grass Species ◽  
Severe Drought ◽  
Hormone Production ◽  
Plant Host ◽  
Bacterial Community Diversity

Grasslands represent a critical ecosystem important for global food production, soil carbon storage, and water regulation. Current intensification and expansion practices add to the degradation of grasslands and dramatically increase greenhouse gas emissions and pollution. Thus, new ways to sustain and improve their productivity are needed. Research efforts focus on the plant-leaf microbiome, or phyllosphere, because its microbial members impact ecosystem function by influencing pathogen resistance, plant hormone production, and nutrient availability through processes including nitrogen fixation. However, little is known about grassland phyllospheres and their response to environmental stress. In this study, globally dominant temperate and tropical forage grass species were grown in a greenhouse under current climate conditions and drought conditions that mimic future climate predictions to understand if (i) plant host taxa influence microbial community assembly, (ii) microbial communities respond to drought stress, and (iii) phyllosphere community changes correlate to changes in plant host traits and stress-response strategies. Community analysis using high resolution sequencing revealed Gammaproteobacteria as the dominant bacterial class, which increased under severe drought stress on both temperate and tropical grasses while overall bacterial community diversity declined. Bacterial community diversity, structure, and response to drought were significantly different between grass species. This community dependence on plant host species correlated with differences in grass species traits, which became more defined under drought stress conditions, suggesting symbiotic evolutionary relationships between plant hosts and their associated microbial community. Further understanding these strategies and the functions microbes provide to plants will help us utilize microbes to promote agricultural and ecosystem productivity in the future.

scholarly journals Transcriptome Profile Analysis of Arabidopsis Reveals the Drought Stress-Induced Long Non-coding RNAs Associated With Photosynthesis, Chlorophyll Synthesis, Fatty Acid Synthesis and Degradation

2021 ◽  
Vol 12 ◽  
Author(s):  
Kang Chen ◽  
Yang Huang ◽  
Chunni Liu ◽  
Yu Liang ◽  
Maoteng Li
Keyword(s):  
Fatty Acid ◽  
Drought Stress ◽  
Fatty Acid Synthesis ◽  
Oil Content ◽  
Acid Synthesis ◽  
Chlorophyll Synthesis ◽  
Short Term ◽  
Non Coding Rnas ◽  
Mutant Lines

Long non-coding RNAs (lncRNAs) play an important role in the response of plants to drought stress. The previous studies have reported that overexpression of LEA3 and VOC could enhance drought tolerance and improve the oil content in Brassica napus and Arabidopsis thaliana, and most of the efforts have been invested in the gene function analysis, there is little understanding of how genes that involved in these important pathways are regulated. In the present study, the transcriptomic results of LEA3 and VOC over-expressed (OE) lines were compared with the RNAi lines, mutant lines and control lines under long-term and short-term drought treatment, a series of differentially expressed lncRNAs were identified, and their regulation patterns in mRNA were also investigated in above mentioned materials. The regulation of the target genes of differentially expressed lncRNAs on plant biological functions was studied. It was revealed that the mutant lines had less drought-response related lncRNAs than that of the OE lines. Functional analysis demonstrated that multiple genes were involved in the carbon-fixing and chlorophyll metabolism, such as CDR1, CHLM, and CH1, were regulated by the upregulated lncRNA in OE lines. In LEA-OE, AT4G13180 that promotes the fatty acid synthesis was regulated by five lncRNAs that were upregulated under both long-term and short-term drought treatments. The key genes, including of SHM1, GOX2, and GS2, in the methylglyoxal synthesis pathway were all regulated by a number of down-regulated lncRNAs in OE lines, thereby reducing the content of such harmful compounds produced under stress in plants. This study identified a series of lncRNAs related to the pathways that affect photosynthesis, chlorophyll synthesis, fatty acid synthesis, degradation, and other important effects on drought resistance and oil content. The present study provided a series of lncRNAs for further improvement of crop varieties, especially drought resistant and oil content traits.

scholarly journals Primed Acclimation of Papaya Increases Short-term Water Use But Does Not Confer Long-term Drought Tolerance

HortScience ◽  
2017 ◽  
Vol 52 (3) ◽  
pp. 441-449 ◽  
Author(s):  
Christopher Vincent ◽  
Diane Rowland ◽  
Bruce Schaffer
Keyword(s):  
Drought Stress ◽  
Soil Water ◽  
Water Deficit ◽  
Water Use ◽  
Dry Matter ◽  
Leaf Gas Exchange ◽  
Severe Drought ◽  
Dry Matter Accumulation ◽  
Short Term ◽  
Soil Water Tension

Primed acclimation (PA) is a regulated deficit irrigation (RDI) strategy designed to improve or maintain yield under subsequent drought stress. A previous study showed photosynthetic increases in papaya in response to a PA treatment. The present study was undertaken to test the duration of the PA effect when papaya plants were challenged with severe drought stress. Potted plants were stressed at 1, 2, and 3 months after conclusion of a PA treatment consisting of 3 weeks at soil water tension (SWT) of −20 kPa. Measurements included leaf gas exchange, root growth, and organ dry mass partitioning. PA did not reduce net CO2 assimilation (A) during the deficit period. At the end of the PA period, total dry matter accumulation per plant and for each organ was unaffected, but proportional dry matter partitioning to roots was favored. After resuming full irrigation, A increased and whole plant water use was more than doubled in PA-treated plants. However, water use and A of PA-treated plants decreased to reconverge with those of control plants by 6 weeks after the PA treatment. Over the course of the study, PA plants maintained lower stem height to stem diameter ratios, and shorter internode lengths. However, these changes did not improve photosynthetic response to any of the water-deficit treatments. We conclude that papaya exhibits some signs of stress memory, but that rapid short-term acclimation responses dominate papaya responses to soil water deficit.

Caragana korshinskii seedlings maintain positive photosynthesis during short-term, severe drought stress

2011 ◽  
Vol 49 (4) ◽  
pp. 603-609 ◽  
Author(s):  
X. W. Fang ◽  
N. C. Turner ◽  
F. M. Li ◽  
W. J. Li ◽  
X. S. Guo
Keyword(s):  
Drought Stress ◽  
Severe Drought ◽  
Short Term ◽  
Caragana Korshinskii

scholarly journals The impact of drought stress on the yields and food value of selected forage grasses

2016 ◽  
Vol 69 (2) ◽  
Author(s):  
Mariola Staniak
Keyword(s):  
Soil Moisture ◽  
Water Stress ◽  
Drought Stress ◽  
Dry Matter ◽  
Nutritional Value ◽  
Lolium Multiflorum ◽  
Grass Species ◽  
Forage Grasses ◽  
The Impact

<p>The aim of the study was to compare yields and nutritional value of selected species and cultivars of forage grasses under the optimal moisture conditions and long-term drought stress. The regenerative capacity of plants after dehydration was also assessed. The pot experiment was conducted in years 2009–2010 in IUNG-PIB’s greenhouse in Puławy, Poland. Nine cultivars of four species: <em>Dactylis glomerata</em> (‘Amera’, ‘Minora’), <em>Festuca pratensis</em> (‘Skra’, ‘Fantazja’), <em>Festulolium braunii</em> (‘Felopa’, ‘Agula’, ‘Sulino’), and <em>Lolium multiflorum</em> (‘Gisel’, ‘Lotos’) were investigated in well-watered conditions (70% field water capacity – FWC) and under a long-term drought stress (40% FWC).</p><p>The study showed that stress caused by soil moisture deficiency significantly reduced yields of <em>D. glomerata</em>, <em>F. pratensis</em>, <em>F. braunii</em>, and <em>L. multiflorum</em>. The total yield of dry matter under stress conditions was about 31% lower, compared to the performance achieved on the optimally moisturized treatment. The smallest reduction in dry matter yield under the conditions of water deficit was recorded for <em>D. glomerata</em>, which makes it the most resistant to stress, followed by <em>F. pratensis</em>. The resistance of <em>F. braunii</em> and <em>L. multiflorum</em> to stress was similar and significantly lower. There was a various response of different grasses to the water stress. On the basis of the value of the DSI (drought susceptibility index), the tested cultivars were ranked depending on the sensitivity to drought, starting with the most resistant cultivar: ‘Minora’, ‘Skra’, ‘Fantazja’, ‘Amera’, ‘Sulino’, ‘Agula’, ‘Gisel’, ‘Lotos’, and ‘Felopa’. The digestibility of dry matter and nutrient value of the grasses depended on both the level of soil moisture and grass species. Under the water stress, the digestibility and protein value increased compared to the control objects. <em>Lolium multiflorum</em> and <em>F. braunii</em> had the best nutritional value, while <em>D. glomerata</em> – the weakest.</p>

scholarly journals Long-term watershed management is an effective strategy to reduce organic matter export and disinfection by-product precursors in source water

2019 ◽  
Vol 28 (10) ◽  
pp. 804 ◽  
Author(s):  
Hamed Majidzadeh ◽  
Huan Chen ◽  
T. Adam Coates ◽  
Kuo-Pei Tsai ◽  
Christopher I. Olivares ◽  
...  
Keyword(s):  
Organic Matter ◽  
South Carolina ◽  
Watershed Management ◽  
Management Practices ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Long Term Effects ◽  
Pyrolysis Gas ◽  
Water Extracts

Watershed management practices such as prescribed fire, harvesting and understory mastication can alter the chemical composition and thickness of forest detritus, thus affecting the quantity and quality of riverine dissolved organic matter (DOM). Long-term effects of watershed management on DOM composition were examined through parallel field and extraction-based laboratory studies. The laboratory study was conducted using detritus samples collected from a pair of managed and unmanaged watersheds in South Carolina, USA. Results showed that dissolved organic carbon (DOC), total dissolved nitrogen (TDN) and ammonium (NH4+-N) concentrations were higher in water extracts from the unmanaged watershed than from the managed watershed (P&lt;0.01). Pyrolysis gas chromatography–mass spectrometry analysis showed that water extracts from the unmanaged watershed contained more aromatic compounds than extracts from the managed watershed. For the field study, monthly water samples were collected for 1 year (2015) from the paired watersheds. DOC and TDN concentrations, as well as DOM aromaticity, were significantly higher in the unmanaged watershed than in the managed watershed for most of the year (P&lt;0.05) and were linked to detrital thickness, precipitation and flow patterns. The formation potential of two regulated disinfection by-products was lower in the unmanaged watershed for most of 2015 (P&lt;0.05). From this study, it appears that long-term watershed management practices may alter detrital mass and chemistry in ways that improve water quality.

scholarly journals The effect of short-term vs. long-term soil moisture stress on the physiological response of three cocoa (Theobroma cacao L.) cultivars

2020 ◽  
Vol 92 (2) ◽  
pp. 295-306 ◽  
Author(s):  
Wiebke Niether ◽  
Alexandra Glawe ◽  
Katharina Pfohl ◽  
Noah Adamtey ◽  
Monika Schneider ◽  
...  
Keyword(s):  
Soil Moisture ◽  
Theobroma Cacao ◽  
Moisture Stress ◽  
Biochemical Response ◽  
P Value ◽  
Severe Drought ◽  
Seedling Mortality ◽  
Short Term ◽  
Soil Moisture Stress

Abstract Understanding water stress signaling mechanisms and screening for tolerant cocoa cultivars are major challenges when facing prolonged dry and rainy seasons in cocoa-producing areas. While abscisic acid (ABA) and proline are supposed to enhance drought tolerance in cocoa, the role of polyamines remains unclear. The aim of this study was to investigate the biochemical response and phenological adaptation of cocoa (Theobroma cacao) on different soil moisture conditions, with a focus on short-term (20 days) and long-term (89 days) stress conditions, and to compare the performance of three cocoa cultivars. In a split plot design with four blocks, cocoa seedlings of an international high-yielding cultivar (TSH-565) and two locally selected cultivars (IIa-22 and III-06) from the drought-exposed Alto Beni region, Bolivia, were arranged in pots under a roof shelter (cultivar: three levels). The seedlings were exposed to strong (VERY DRY) and moderate (DRY) soil moisture deficits, water logging (WET) and regular irrigation (MOIST) that served as a control (moisture: four levels). We examined the growth performance and the levels of ABA, proline, and polyamines in the leaves. Growth was reduced already at a moderate drought, while severe drought enhanced seedling mortality. Severe drought increased the levels of ABA by 453% and of proline by 935%, inducing a short-term stress response; both compounds were degraded over the long-term period. The polyamine concentration was unrelated to soil moisture. The cocoa cultivars did not differ in their biochemical response to soil moisture stress (proline: p-value = 0.5, ABA: p-value = 0.3), but the local cultivar III-06 showed a stronger height growth increment than the international cultivar TSH-565 (237%, p-value = 0.002) under drought conditions.

scholarly journals Proteins Involved in Energy Metabolism and Oxidative Regulation Associated with Genotypic Variations in Drought Tolerance for Tall Fescue

2018 ◽  
Vol 143 (3) ◽  
pp. 207-212
Author(s):  
Jianming Sun ◽  
Yiming Liu ◽  
Xianglin Li ◽  
Bingru Huang
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Tall Fescue ◽  
Photochemical Efficiency ◽  
Grass Species ◽  
Drought Treatment ◽  
Drought Tolerant ◽  
Oxidative Regulation ◽  
Tolerant Genotype ◽  
Adaptation To Drought

Protein metabolism plays an important role in plant adaptation to drought stress. The objective of this study was to identify drought-responsive proteins associated with differential drought tolerance for a tolerant genotype (RU9) and a sensitive genotype (RU18) of tall fescue (Lolium arundinacea). Plants of both genotypes were grown under well-watered conditions or subjected to drought stress by withholding irrigation for 12 days in a growth chamber controlled at the optimal growth temperatures of 23/18 °C (day/night). Physiological analysis demonstrated that RU9 was relatively more drought tolerant than RU18, as shown by the higher leaf net photosynthetic rate (Pn) and photochemical efficiency at 12 days of drought treatment. Differentially expressed proteins between RU9 and RU18 exposed to drought stress were identified by two-dimensional electrophoresis and mass spectrometry (MS). Several proteins [photosystem I reaction center subunit II, Rubisco small subunit, and Glyceraldehyde-3-phosphate dehydrogenase (GADPH)] in photosynthesis, respiration, or oxidative regulation exhibited higher abundance in RU9 than RU18 under drought stress. These results suggested the critical importance of energy and oxidative metabolism in tall fescue adaptation to drought stress. Those abundant proteins in the drought-tolerant genotype could be used as biomarkers or developed to molecular markers to develop elite drought-tolerant germplasm in tall fescue and other cool-season perennial grass species.

scholarly journals Responses of isoprene emission and photochemical efficiency to severe drought combined with prolonged hot weather in hybrid Populus

2020 ◽  
Vol 71 (22) ◽  
pp. 7364-7381
Author(s):  
Zhihong Sun ◽  
Yan Shen ◽  
Ülo Niinemets
Keyword(s):  
Emission Rate ◽  
Photochemical Efficiency ◽  
Photosynthetic Apparatus ◽  
Photosynthetic Characteristics ◽  
Antioxidant Enzyme Activities ◽  
Severe Drought ◽  
Effective Quantum Yield ◽  
Isoprene Emission ◽  
Hot Weather

Abstract Isoprene emissions have been considered as a protective response of plants to heat stress, but there is limited information of how prolonged heat spells affect isoprene emission capacity, particularly under the drought conditions that often accompany hot weather. Under combined long-term stresses, presence of isoprene emission could contribute to the maintenance of the precursor pool for rapid synthesis of essential isoprenoids to repair damaged components of leaf photosynthetic apparatus. We studied changes in leaf isoprene emission rate, photosynthetic characteristics, and antioxidant enzyme activities in two hybrid Populus clones, Nanlin 1388 (relatively high drought tolerance) and Nanlin 895 (relatively high thermotolerance) that were subjected to long-term (30 d) soil water stress (25% versus 90% soil field capacity) combined with a natural heat spell (day-time temperatures of 35–40 °C) that affected both control and water-stressed plants. Unexpectedly, isoprene emissions from both the clones were similar and the overall effects of drought on the emission characteristics were initially minor; however, treatment effects and clonal differences increased with time. In particular, the isoprene emission rate only increased slightly in the Nanlin 895 control plants after 15 d of treatment, whereas it decreased by more than 5-fold in all treatment × clone combinations after 30 d. The reduction in isoprene emission rate was associated with a decrease in the pool size of the isoprene precursor dimethylallyl diphosphate in all cases at 30 d after the start of treatment. Net assimilation rate, stomatal conductance, the openness of PSII centers, and the effective quantum yield all decreased, and non-photochemical quenching and catalase activity increased in both control and water-stressed plants. Contrary to the hypothesis of protection of leaf photosynthetic apparatus by isoprene, the data collectively indicated that prolonged stress affected isoprene emissions more strongly than leaf photosynthetic characteristics. This primarily reflected the depletion of isoprene precursor pools under long-term severe stress.

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