scholarly journals Free radical damage: role in varicella zoster virus infection

2021 ◽  
pp. 41-47
Author(s):  
Olga Volobuieva ◽  
Tetiana Liadova ◽  
Mykola Popov ◽  
Olga Sorokina ◽  
Olena Ognivenko ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Infectious Diseases ◽  
Blood Plasma ◽  
Red Blood Cells ◽  
Antioxidant System ◽  
Blood Cells ◽  
The State ◽  
Free Radical Damage ◽  
Acute Period

Study of the role of lipid peroxidation and antioxidant system in patients with infectious diseases is of great interest to researchers. Numerous studies have led to a common understanding of their contribution to the pathogenesis of infectious diseases, as well as to the complications development. However, the state of lipid peroxidation and antioxidant protection in patients with chickenpox (varicella) has not been sufficiently studied which is important for the development of new targeted treatments. The aim of the research - to assess the state of lipid peroxidation (LPO) and the antioxidant system (AOS) in the dynamics in patients with chickenpox, depending on the severity of the disease. Materials and methods. We selected for participating in the study 240 chickenpox patients (142 women and 98 men) aged 18-40 years. Chickenpox was diagnosed using clinical, serological, and molecular genetic methods. The state assessment of LPO in chickenpox patients was based on the determination of serum diene conjugates (DC), malondialdehyde (MDA) levels and the total oxidative activity (TOA) of blood plasma. Results. Study showed that the process of lipid peroxidation increases and the activity of the antioxidant system decreases in patients with chickenpox during the acute period of the disease. The extent of these processes becomes greater as the severity of chickenpox increases. A decrease in the activity of antioxidant enzymes and a disruption in the functioning of the glutathione system in patients with moderate to severe hypertension leads to disruption of protective mechanisms and, as a result, to an increase in the free radical chain reactions, the uncontrolled growth of which causes irreversible damage to the membranes of various cells, which underlies visceropathy in chickenpox patients. Conclusions. Antioxidant system is activated in the acute period in patients with mild chickenpox. It is evidenced by an increase in the overall antioxidant activity of blood plasma (p=0.045) and red blood cells (p=0.00087), in the activity of catalase (p=0.001), superoxide dismutase (p=0.0093), glutathione peroxidase (p=0.036), glutathione reductase of plasma and red blood cells, and an increase in the concentration of oxidized and reduced glutathione in blood. Lipid peroxidation is hyperactivated in the acute period in patients with a moderate and severe course of the disease and an excess amount of primary and secondary hydroperoxides of fatty acids accumulates in the blood. This is accompanied by indicates a developing imbalance between the oxidative and antioxidant systems in group of patients with moderate and severe chickenpox. Our findings confirm the usefulness of antioxidants in the treatment of chickenpox.

scholarly journals Oxidative stress in osteomyelitis and burns: Specific features of its role in development of pathology

2021 ◽  
Vol 8 (3) ◽  
pp. 4286-4293
Author(s):  
Andrew K. Martusevich ◽  
Valery I. Zagrekov ◽  
Ksenia L. Belyaeva ◽  
Alexander S. Pushkin ◽  
Anna G. Soloveva
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Blood Plasma ◽  
Red Blood Cells ◽  
Antioxidant System ◽  
Oxidative Metabolism ◽  
Blood Cells ◽  
Chronic Osteomyelitis ◽  
Wide Range ◽  
In Blood Plasma

Introduction: The purpose of this study was to evaluate and compare the features of the initiation and development of oxidative stress in patients with osteomyelitis and burns. Methods: We studied the oxidative metabolism of blood of 20 healthy subjects (controls), 15 patients with burns, and 18 patients with chronic osteomyelitis. All patients included in the second group had thermal burns of the I-II-III degree in trunk and limbs on an area of 31-80% of the body surface without thermal inhalation trauma. After standard sample preparation, a wide range of parameters of oxidative metabolism was determined in the blood. The intensity of free radical processes in blood plasma and red blood cells, and the total antioxidant activity was evaluated by Fe-induced biochemiluminescence. The concentration of malonic dialdehyde in blood plasma and red blood cells was determined. The level of diene and triene conjugates and Schiff bases was determined spectrophotometrically using reagent kits. The catalase and superoxide dismutase activities in the red blood cells of patients from each of the groups was also determined. Results: We showed that in osteomyelitis, which is a long-lasting process, changes in the balance of free radical generation and activity of the antioxidant system were compensatory and mostly related to changes in blood plasma. On the contrary, in burn victims, oxidative stress signs had a maladaptive character. They were seen in blood plasma and red blood cells, and accompanied by a pronounced depletion of enzyme antioxidant system reserves. Conclusion: Our study demonstrate the role of oxidative stress in patients with burns and chronic osteomyelitis, and demonstrate some specific features leading to formation of disease pathology. Such features of oxidative stress may be useful in future design of new approaches to correct the pathology of diseases.

scholarly journals Forms of pseudotuberculosis progression in children and the impact of lipid peroxidation activation and antioxidant system imbalance on their development

2021 ◽  
Vol 12 (5) ◽  
pp. 130-137
Author(s):  
A. P. Pomogaeva ◽  
O. L. Nosareva ◽  
E. A. Stepovaya ◽  
T. V. Zhavoronok ◽  
E. V. Shakhristova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Blood Plasma ◽  
Red Blood Cells ◽  
Disease Progression ◽  
Antioxidant System ◽  
Blood Cells ◽  
Reduced Glutathione ◽  
Recovery Period ◽  
Lipid Peroxidation Products ◽  
The Impact

The objective is to compare clinical symptoms, lipid peroxidation indicators, the state of the antioxidant system and assess their impact on the severity and progression of pseudotuberculosis in children.Materials and methods. We examined 125 children with pseudotuberculosis divided into 4 groups according to the severity and nature of the disease progression and 45 healthy children. The material for the study was red blood cells and blood plasma of patients received in the dynamics – the acute period (during hospitalization); 3-4 weeks later – the phase of early convalescence with a non-smooth progression and moderate and heavy severity; the recovery period with a smooth progression and mild and moderate severity; 5-6 weeks later – the recovery period with a non-smooth progression and moderate and heavy severity. The spectrophotometric method was used to study lipid peroxidation (the concentration of diene conjugates, TBA-reactive substances) in the blood plasma and components of the antioxidant support system (the content of reduced glutathione; the activity of glutathione reductase, glutathione peroxidase, and catalase) in red blood cells.Results. It was determined that moderate and heavy pseudotuberculosis forms prevail in hospitalized children, the disease progression in 35.2% of them was non-smooth; lipid peroxidation products accumulate in the blood plasma and the concentration of reduced glutathione decreases in red blood cells during the acute period of pseudotuberculosis in all children relative to the parameters in the control group. In the period of early convalescence an imbalance in the functioning of antioxidant enzymes of red blood cells, as well as the accumulation of TBA-reactive substances and a decrease in the content of reduced glutathione were observed in patients with moderate and heavy pseudotuberculosis.Conclusion. The impact of the imbalance of pro-/antioxidants on the formation of predominantly moderate and heavy pseudotuberculosis in children is shown. Prognostic criteria for the development of a non-smooth progression of pseudotuberculosis are a high level of lipid peroxidation products in the blood plasma, no normalization in values of glutathione system components and the activity of erythrocyte catalase during early convalescence. 

scholarly journals THE STATE OF THE ANTIOXIDANT SYSTEM OF THE INTERNAL ORGANS IN RATS DURING BURN DISEASE

2014 ◽  
Vol 13 (3) ◽  
pp. 51-55
Author(s):  
L. G. Netyukhailo ◽  
T. A. Sukhomlin ◽  
Ya. A. Basarab ◽  
V. V. Bondarenko ◽  
S. V. Kharchenko
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Salivary Glands ◽  
Antioxidant System ◽  
The State ◽  
Antioxidant Systems ◽  
Free Radical Processes ◽  
Burn Disease ◽  
Radical Processes

The objective of research was to study the state of prooxidant and antioxidant systems in the tissues of the lungs, kidneys, pancreas and salivary glands at burn disease. The intensity of the free radical processes was evaluated on the basis of the content of malondialdehyde (MDA) and antioxidant system – based on the indexes of its enzymatic chain: superoxide dismutase and catalase in homogenates of the studied organs. It has been found that changes in experimental burn disease depend on the studied organs and the stage of burn disease. The activation of free radical processes observed in all investigated organs (lungs, kidneys, pancreas and salivary glands). Reactive oxygen species induce lipid peroxidation, which is a universal marker of tissue damage. MDA appears in the body during degradation of polyunsaturated fatty acids and it’s a marker of lipid peroxidation and oxidative stress. It was found the increasing of MDA in all organs, especially in the lungs and kidneys at stage of burn shock. Under these conditions it was observed the decrease of superoxide dismutase and catalase in all investigated organs. At burn disease there is development of disbalance between the action of prooxidant and antioxidant systems due to the activation of free radical processes.

scholarly journals Assessment of the state of the oxidant-antioxidant system and lipid peroxidation in patients with chronic pancreatitis combined with hypothyroidism

2021 ◽  
Vol 25 (3 (99)) ◽  
pp. 97-101
Author(s):  
V. Ratsa ◽  
O. Fedіv
Keyword(s):  
Lipid Peroxidation ◽  
Chronic Pancreatitis ◽  
Blood Plasma ◽  
Antioxidant System ◽  
Underlying Disease ◽  
The State ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
In Blood Plasma

Changes in oxidative-antioxidant homeostasis is one of the pathogenetic links in the onset and progression of many nosologies, including chronic pancreatitis (CP) and hypothyroidism (HТ), however, data on the development of oxidative stress (OS) with a combination of CP and HP are insufficient, therefore, determining the role of these systems with a comorbid course of these pathologies is an urgent issue of our time.The aim of the work is to study the state of the oxidative and antioxidant system in patients with CP and CP combined with HT.Material and methods. 96 people were examined, including 26 patients with chronic pancreatitis (group 1), 24 patients with hypothyroidism (group 2), 26 patients with chronic pancreatitis on the background of hypothyroidism (group 3), 20 almost healthy individuals (group 4). Evaluation of the oxidative-antioxidant system and lipid peroxidation was assessed by determining the content of malonic aldehyde in blood plasma (MA pl.) And erythrocytes (MA er.), The level of reduced glutathione, glutathione peroxidase (GP) and glutathione-S-trans-ion (GT). Enzyme activity was calculated per 1 g of hemoglobin (HB).Research results. The analysis of the obtained results revealed clear patterns of changes in the state of the oxidative-antioxidant system and lipid peroxidation in patients with chronic pancreatitis on the background of hypothyroidism. Namely, violation of lipid peroxidation in patients with comorbid pathology, accompanied by an increase in the content of MA er. by 44.87% and the content of MA in blood plasma by 46.42%; oxidative-antioxidant system: decrease in HF in the blood by 51,61%, combined of a compensatory increase in BP - by 48.03% and HT - by 34.76%.Conclusion. Under the conditions of comorbidity of CP and HT, there is a violation of the oxidative-antioxidant system and the state of lipid peroxidation, which is characterized by a significant increase in MA in erythrocytes and MA in plasma against a decrease in glutathione and which complicates the course of the underlying disease, its diagnosis and treatment.

scholarly journals Antioxidant system of rats with heavy metals and phospoorganic pesticides intoxication and peptides applying

2015 ◽  
Vol 17 (3) ◽  
Author(s):  
Ye. B. Dmukhalska ◽  
Ya. I. Honsky
Keyword(s):  
Heavy Metals ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Enzymes ◽  
Free Radical ◽  
Antioxidant System ◽  
The State ◽  
Antioxidant Protection ◽  
Free Radical Processes ◽  
Radical Processes

<p>The effect of peptide tsysteil-histidil-tyrosil-histidil-isoleucine on the state of antioxidant protection (superoxide<br />dismutase, catalase) and lipid peroxidation has been reached. The peptide exhibits antioxidant activity, the correction<br />of the peptide reduces free radical processes and decreases the products of lipid peroxidation and increases<br />antioxidant enzymes activity.</p>

scholarly journals Using redox potential as a feasible marker for banked blood quality and the state of oxidative stress in stored red blood cells

2021 ◽  
Author(s):  
Rodney C. Daniels ◽  
Hyesun Jun ◽  
Robertson D. Davenport ◽  
Maryanne M. Collinson ◽  
Kevin R. Ward
Keyword(s):  
Oxidative Stress ◽  
Redox Potential ◽  
Red Blood Cells ◽  
Blood Cells ◽  
The State

scholarly journals Synthesis and Antioxidant of Some New Pyrazolo[1,5-a]pyrimidine, Pyrazolo[5,1-b]quinazoline and Imidazo[1,2-b]pyrazole Derivatives Incorporating Phenylsulfonyl Moiety

2021 ◽  
Vol 10 (3) ◽  
pp. 2414-2428
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Protective Effects ◽  
Oxidative Hemolysis ◽  
Elemental Analyses ◽  
Phenolic Group ◽  
High Antioxidant Activity ◽  
New Compounds

The synthesis and antioxidant of some new pyrazole analogs were described. It is achieved by the reaction of phenyl-4-(phenylsulfonyl)-1H-pyrazole-3,5-diamine (3) with different bifunctional reagents. The free radical-induced damage and the protective effects of the newly synthesized pyrazoles were studied. These new compounds inhibit the free radical-induced oxidative hemolysis of red blood cells effectively. It was found that these compounds effectively inhibit the free radical-induced oxidative hemolysis of red blood cells. Compound 5, which contain phenolic group, and 17, which bear sulfur, nitrogen atoms, and benzothiazole ring, respectively displayed high antioxidant activity. Analogs 15, 11, 10, and 9 were proved to exhibit antioxidative activity. Structures of new pyrazoles were confirmed by spectroscopic and elemental analyses and have been screened for their antioxidant activity.

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