Acute exposure to crotonaldehyde induces dysfunction of immune system in male Wistar rats

Introduction: The use of antiepileptic drugs (AEDs) such as carbamazepine and phenytoin are part of strategies for the management of epilepsy. Acute exposure of epileptic patients to AEDs can cause sensory impairment. Aim: This study seeks to assess sensorimotor changes in male Wistar rats upon single-large dose exposure to carbamazepine, phenytoin and their mixture. Methods: 24 male Wistar rats (160-210 g) were randomly separated to four groups with 6 rats each. Groups I, II and III was given distilled water (2 ml/kg), carbamazepine (1950 mg/kg); and phenytoin (820 mg/kg) respectively, while Group IV (CBZ+PHY) was co-exposed to carbamazepine (1950 mg/kg) and phenytoin (820 mg/kg). The treatment was orally administered once by gavage (on Day(D) 1), then followed by weekly monitoring of body weight, clinical signs and neurobehavioural parameters for four weeks (D0, D1, D7, D14, D21 and D28). Results: The body weight revealed insignificant improvement (p > 0.05) in all groups. A significantly (p < 0.05) lower grooming frequency, increased locomotor activity and a reduction in the frequency of urination and defecation were recorded in the CBZ and PHY groups. Also, the number of missed rungs, inclined plane and grip fore-paw time reduced significantly (p < 0.05) in CBZ, PHY and CBZ+PHY groups. Significance: A single large dose of CBZ, PHY and their combination caused anxiogenic and sensorimotor impairment.

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ABNORMALITY IN LEARNING ON THE MODEL OF PARKINSON-LIKE SYNDROME: THE CORRECTABILITY BY THYMUS PEPTIDES

Medical Journal of the Russian Federation ◽

10.18821/0869-2106-2018-24-6-310-315 ◽

2018 ◽

Vol 24 (6) ◽

pp. 310-315

Author(s):

A. G Korolev ◽

A. V Novoseletskaya ◽

N. M Kiseleva

Keyword(s):

Parkinson’S Disease ◽

Immune System ◽

Wistar Rats ◽

Peptide Drugs ◽

Immune Systems ◽

Avoidance Test ◽

Early Stages ◽

Memory Footprint ◽

Male Wistar Rats ◽

Bidirectional Interactions

In recent times the question of the bidirectional interactions of the neuroendocrine and immune systems is studied quite actively, its mechanisms offer the opportunity to allow for optimization the therapy of several diseases, including Parkinson’s disease. The purpose of this work is to study the influence of peptide drugs derived from the body’s immune system, the thymus, for the avoidance test conditioning, both in health and in the model of Parkinson-like syndrome. The work was performed on 80 male Wistar rats weighing 300-350 g. The injection of thymosin fraction 5 and thymalin to animals corrects clinical traits and behavioral dysfunctions caused by the singular injection of the neurotoxin. It also facilitates conditioned active evasion response in rats, which is especially noticeable at the early stages of memory footprint formation.

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Gestational exposure to the cannabinoid WIN 55,212-2 and its effect on the innate intestinal immune response

Scientific Reports ◽

10.1038/s41598-019-56653-y ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Rosalía Hernández-Cervantes ◽

Armando Pérez-Torres ◽

Óscar Prospéro-García ◽

Jorge Morales Montor

Keyword(s):

Immune System ◽

Wistar Rats ◽

Trichinella Spiralis ◽

Intestinal Parasites ◽

Control Group ◽

Prenatally Exposed ◽

Mucus Production ◽

Gestational Exposure ◽

Male Wistar Rats ◽

Marijuana Consumption

AbstractThe consequences of marijuana consumption during pregnancy and its effects on the function of the immune system have been little studied. Marijuana is one of the most consumed recreational drugs among pregnant women, and it is known that gestational exposure to marijuana can have serious effects on the offspring after birth. In this study, we challenged the immune system of Wistar rats by infecting them with the parasitic nematode Trichinella spiralis. A treatment group of these animals was prenatally exposed to the cannabinoid WIN 55,212-2; a control group was not exposed. At 5 days of infection, the treated animals were less effective in eliminating intestinal parasites; moreover, this effect was correlated with a deficiency in mucus production, lower recruitment of eosinophils in the duodenum, and a reduced percentage of Tγδ and NK cells. In conclusion, the gestational administration of the synthetic cannabinoid WIN 55,212-2 induces lasting changes to the function of the immune system against infection with T. spiralis in male Wistar rats, making them more susceptible to infection.

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Influence of the elevated ambient temperature on immunoglobulin G and immunoglobulin G subclasses in sera of Wistar rats

Vojnosanitetski pregled ◽

10.2298/vsp0306657j ◽

2003 ◽

Vol 60 (6) ◽

pp. 657-661 ◽

Cited By ~ 1

Author(s):

Maja Jurhar-Pavlova ◽

Aleksandar Petlichkovski ◽

Dejan Trajkov ◽

Olivija Efinska-Mladenovska ◽

Todor Arsov ◽

...

Keyword(s):

Immune System ◽

Ambient Temperature ◽

Immunoglobulin G ◽

Wistar Rats ◽

Control Group ◽

Immunoglobulin G Subclasses ◽

Male Wistar Rats ◽

Regulation Mechanisms ◽

Experimental Group ◽

Igg1 Level

The aim of our research was to examine changes in the immune system of the rats influenced by the elevated ambient temperature. Male Wistar rats were divided, into 2 groups and housed at 20 ? 2?C (n=64, control group) and 35 ? 1?C (n=74, experimental group), during precise timing of 1, 4, 7, 14, 21, and 30 days. All the animals were given food and water ad libitum, and were lighted during 12 hours per day. We have measured IgG, IgG1, IgG2a, IgG2b and IgG2c. The obtained results showed significant elevation in the level of IgG after 4 and 7 days (+32%), IgG2a after 7th (+88%), 14th and 21nd day (+110%), IgG2b after 14 days (+60%) at 35 ? 1?C compared with the control group at 20 ? 2?C. IgG1 level was not affected and IgG2c showed significant decrease after 21st day at 35 ? 1?C. In conclusion, during the elevated ambient temperature the immune system is activated as one of the regulation mechanisms in homeostasis and survival of the population.

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Structural and functional renal alterations in five-sixth nephrectomized rats on unrestricted diet

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010014333x ◽

1986 ◽

Vol 44 ◽

pp. 342-343

Author(s):

I. Stachura ◽

M. Pardo ◽

J. Costello ◽

D.M. Landwehr

Keyword(s):

Wistar Rats ◽

Renal Damage ◽

Standard Diet ◽

Experimental Conditions ◽

Phosphate Intake ◽

Dietary Restrictions ◽

Severe Reduction ◽

Male Wistar Rats ◽

Unrestricted Diet ◽

Progressive Renal Insufficiency

Under experimental conditions severe reduction of renal mass results in the hyperfiltration of the remaining nephrons leading to a progressive renal insufficiency. Similar changes are observed in patients with various renal disorders associated with a loss of the functioning nephrons. The progression of renal damage is accelerated by high protein and phosphate intake, and may be modified by the dietary restrictions.We studied 50 five-sixth nephrectarrized male Wistar rats on a standard diet (Rodent Laboratory Chow 5001 Ralston Purina Co., Richmond, Indiana; containing 23.4% protein) over a 20 week period.

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Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000654 ◽

2020 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Masoud Nasiri ◽

Saja Ahmadizad ◽

Mehdi Hedayati ◽

Tayebe Zarekar ◽

Mehdi Seydyousefi ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Wistar Rats ◽

Enzymatic Antioxidants ◽

Acute Response ◽

Total Antioxidant ◽

Male Wistar Rats ◽

Exercise Induced ◽

And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

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