scholarly journals ALTERNATIVE METHODS OF BIOMODELING FOR FUNCTIONAL FOOD PRODUCTS EFFECTIVENESS RESEARCH

Food systems ◽  
2018 ◽  
Vol 1 (3) ◽  
pp. 33-37
Author(s):  
Liliya V. Fedulova ◽  
Ekaterina R. Vasilevskaya ◽  
Elena A. Kotenkova ◽  
Elta B. Kashinova
Keyword(s):  
Functional Food ◽  
Ex Vivo ◽  
Raw Materials ◽  
Meat Products ◽  
Alternative Methods ◽  
Animal Origin ◽  
Tissue Cultures ◽  
Basic Principles ◽  
In Vitro Methods

The article describes in vitro methods basic principles, authors analyzed cell and tissue cultures used to assess toxicity and specific biological activity, including metabolic processes, include antihypertensive and cytoprotective properties analysis, antioxidant activity (in vitro and ex vivo) used to study ingredients functional properties based on animal origin raw materials, as well as meat products.

scholarly journals Prevalence of Listeria monocytogenes in milk, meat and foodstuff of animal origin and the phenotype of antibiotic resistance of isolated strains 

2012 ◽  
Vol 49 (No. 7) ◽  
pp. 243-252 ◽  
Author(s):  
P. Navratilova ◽  
J. Schlegelova ◽  
A. Sustackova ◽  
E. Napravnikova ◽  
J. Lukasova ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Raw Materials ◽  
Meat Products ◽  
Raw Milk ◽  
Production Equipment ◽  
Animal Origin ◽  
Antimicrobial Drugs ◽  
Antimicrobial Substances ◽  
Microdilution Method

In 2000&ndash;2002 samples of raw materials (milk and beef, 518 samples), ready-to-cook foods and final products from milk (30 and 200 samples) and from meat (105 and 110 samples) and swabs from surfaces in two meat-processing plants (41 samples) were examined for the presence of Listeria monocytogenes (L. monocytogenes). 70 isolates were tested using the standard microdilution method for the susceptibility to 12 antimicrobial drugs, minimum inhibitory concentration (MIC) characteristics (MIC<sub>m</sub>, MIC<sub>50</sub>, MIC<sub>90</sub>) were determined. L. monocytogenes was isolated from raw milk samples (15 samples). It was not isolated from any of the semi-finished and final milk products except for one sample of pasteurized milk. Furthermore L. monocytogenes was isolated from samples of raw beef, meat emulsion, fermented dry meat products and from swabs from production equipment. In vitro testing of susceptibility showed the considerable effectiveness of examined antimicrobial substances with streptomycin and norfloxacin being least effective (MIC<sub>m</sub> = 4 &mu;g/ml). Apart from the intermediate resistance (MIC = 1&ndash;2 &mu;g/ml) and resistance (MIC = 4 &mu;g/ml) to clindamycin (37 and 5 strains, respectively), all strains were susceptible to the tested substances. While the presence of L. monocytogenes in foodstuffs, in particular, are serious, current tests of susceptibility of L. monocytogenes isolates indicate low probability untreatable infections as a result of resistant strains from foods or transfer of resistance to other microorganisms in the CzechRepublic.

The Overt and Hidden Use of Animal-Derived Products in Alternative Methods for Skin Sensitisation: A Systematic Review

2019 ◽  
Vol 47 (5-6) ◽  
pp. 174-195 ◽  
Author(s):  
Bianca Marigliani ◽  
Felipe Perraro Sehn ◽  
Josemar Vinicius Maiworm Abreu Silva ◽  
Luciene Bottentuit López Balottin ◽  
Elisabeth de Fatima Pires Augusto ◽  
...  
Keyword(s):  
Systematic Review ◽  
Safety Assessment ◽  
Alternative Methods ◽  
Animal Origin ◽  
Animal Testing ◽  
Complete Replacement ◽  
In Vitro Methods ◽  
Skin Sensitisation ◽  
Animal Alternatives

In vitro methods that can replace animal testing in the identification of skin sensitisers are now a reality. However, as cell culture and related techniques usually rely on animal-derived products, these methods may be failing to address the complete replacement of animals in safety assessment. The objective of this study was to identify the animal-derived products that are used as part of in vitro methods for skin sensitisation testing. Thus, a systematic review of 156 articles featuring 83 different in vitro methods was carried out and, from this review, the use of several animal-derived products from different species was identified, with the use of fetal bovine serum being cited in most of the methods (78%). The use of sera from other animals, monoclonal antibodies and animal proteins were also variously mentioned. While non-animal alternatives are available and methods free of animal-derived products are emerging, most of the current methods reported used at least one animal-derived product, which raises ethical and technical concerns. Therefore, to deliver technically and ethically better in vitro methods for the safety assessment of chemicals, more effort should be made to replace products of animal origin in existing methods and to avoid their use in the development of new method protocols.

The EYTEX™ System and the Neutral Red Uptake Inhibition Assay in Cultured Hep G2 Cells as Alternative Methods for In Vivo Eye Irritation Following the EEC Protocol

1990 ◽  
Vol 17 (4) ◽  
pp. 325-333
Author(s):  
Paul J. Dierickx ◽  
Virginia C. Gordon
Keyword(s):  
Neutral Red ◽  
Alternative Methods ◽  
Inhibition Assay ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Eye Irritation ◽  
Uptake Inhibition ◽  
In Vitro Methods ◽  
Neutral Red Uptake

The neutral red uptake inhibition assay and the EYTEX™ system were investigated as alternative methods for the assessment of eye irritation, determined according to the EEC protocol. The 17 test chemicals used were mainly organic solvents. The xenobiotics were applied to Hep G2 cells for 24 hours at different concentrations. Neutral red uptake inhibition was then measured. The results are expressed as the NI50 value, which is the concentration of test compound required to induce a 50% reduction in neutral red uptake. The same chemicals were also tested as coded samples by the EYTEX™ test according to the manufacturer's directions. A nearly identical quantitative correlation was found for both in vitro methods with corneal opacity scores: r = 0.84 for EYTEX™ scores and r = 0.83 for log NI50, expressed in μg/ml. Whilst these correlations are certainly not perfect, it is clear that both in vitro methods can be used as valuable prescreening methods.

Ex Vivo Brain Preparation to Analyze Vocal Pathways of Xenopus Frogs

2021 ◽  
Vol 2021 (9) ◽  
pp. pdb.prot106872
Author(s):  
Ayako Yamaguchi
Keyword(s):  
Neural Activity ◽  
Ex Vivo ◽  
Neural Circuitry ◽  
Neural Basis ◽  
Vocal Production ◽  
Basic Principles ◽  
Electrophysiological Recordings ◽  
The Brain

Understanding the neural basis of behavior is a challenging task for technical reasons. Most methods of recording neural activity require animals to be immobilized, but neural activity associated with most behavior cannot be recorded from an anesthetized, immobilized animal. Using amphibians, however, there has been some success in developing in vitro brain preparations that can be used for electrophysiological and anatomical studies. Here, we describe an ex vivo frog brain preparation from which fictive vocalizations (the neural activity that would have produced vocalizations had the brain been attached to the muscle) can be elicited repeatedly. When serotonin is applied to the isolated brains of male and female African clawed frogs, Xenopus laevis, laryngeal nerve activity that is a facsimile of those that underlie sex-specific vocalizations in vivo can be readily recorded. Recently, this preparation was successfully used in other species within the genus including Xenopus tropicalis and Xenopus victorianus. This preparation allows a variety of techniques to be applied including extracellular and intracellular electrophysiological recordings and calcium imaging during vocal production, surgical and pharmacological manipulation of neurons to evaluate their impact on motor output, and tract tracing of the neural circuitry. Thus, the preparation is a powerful tool with which to understand the basic principles that govern the production of coherent and robust motor programs in vertebrates.

HEMATOPOETIC STEM CELL RESPONSE TO STIMULATION OF EXTRACTS FROM ANIMAL MATERIALS

2016 ◽  
Vol 78 (5-6) ◽  
Author(s):  
Ivan Smirnov ◽  
Victor Keino ◽  
Ksenia Goryacheva ◽  
Alexander Shunk ◽  
Alexander Bondarev ◽  
...  
Keyword(s):  
Raw Materials ◽  
Raw Material ◽  
Animal Origin ◽  
Aqueous Extracts ◽  
Second Stage ◽  
Hematopoetic Stem Cell ◽  
Two Stages ◽  
Stimulation Of

The article presents the results of the research hemostimulating activity of aqueous extracts of antler young Siberean stag and drone larvae homogenate. These substrates were obtained from raw materials of animal origin. Altai Krai andAltaiRepublicare subjects of theRussian Federationwhich is the place of production of the raw material. Experiments were conducted in two stages. The first stage - in vitro, which included a research of experimental substrates on the culture of mouse marrow cells. During the experiments were obtained different results. We counted the number of colonies grown in cell culture for this. The second stage of experimenters - in vivo. It included an assessment of the myeloprotector on model of cytostatic myelosuppression of mice and analysis of bone marrow and peripheral blood.

Development of Potential Alternatives to the Draize Eye Test: The CTFA Evaluation of Alternatives Program. Phase II: Review of Materials and Methods

1992 ◽  
Vol 20 (1) ◽  
pp. 164-171
Author(s):  
Stephen D. Gettings ◽  
Daniel M. Bagley ◽  
Michael Chudkowski ◽  
Janis L. Demetrullas ◽  
Louis C. DiPasquale ◽  
...  
Keyword(s):  
Animal Welfare ◽  
Phase Ii ◽  
Task Force ◽  
Alternative Methods ◽  
Personal Care ◽  
In Vitro Methods ◽  
Ocular Irritation ◽  
Member Company ◽  
Sole Criterion

The CTFA Evaluation of Alternatives Program is a multi-year effort, organised by the CTFA Animal Welfare Task Force, designed to evaluate the performance of currently promising in vitro (alternative) methods to the Draize eye irritancy test. The sole criterion for inclusion of a particular test is that it shows some initial promise as an alternative to the Draize eye test, and that it is under evaluation or development by a participating CTFA member company. Tests are evaluated for their ability to rank and discriminate the ocular irritation potential of prototype cosmetic and personal care formulations compared to the Draize eye test. Test materials and in vitro methods currently under evaluation in Phase II of the CTFA Program are described. Additional tests may be included in subsequent phases of the Program, should it be determined that they show particular promise as replacements for specific types of formulation. Conversely (at the discretion of sponsors), tests may be removed from the Program should initial promise be unfulfilled.

scholarly journals The use of pumpkin crumbs in pate technology and their functional characteristics

2019 ◽  
Vol 21 (92) ◽  
pp. 23-27
Author(s):  
I. M. Basarab ◽  
U. R. Drachuk ◽  
I. S. Romashko ◽  
B. I. Halukh ◽  
I. I. Simonova ◽  
...  
Keyword(s):  
Functional Properties ◽  
Nutritional Value ◽  
Raw Materials ◽  
Meat Products ◽  
Animal Origin ◽  
Partial Replacement ◽  
Meat Industry ◽  
Functional Ingredient ◽  
Organoleptic Characteristics ◽  
Specialized Equipment

One of the priorities of the modern meat industry is the production of products that have improved consumer properties. To accomplish this task, it is necessary to involve as much as possible in industrial circulation the local raw materials of plant origin, which will be an impetus for the production of new foods with functional properties. The article deals with the partial replacement of raw materials of animal origin with pumpkin pulp as a functional ingredient in the production of pâté products. We have developed three recipes for pumpkin paste. Changes in nutritional value of the developed samples were investigated. The organoleptic characteristics and nutritional value of pumpkin pate products were determined. The use of pumpkin pulp as a functional ingredient in the technology of pate products affects the appearance, the color of the products and the composition of these products, along with other technological indicators were within the regulatory requirements. Experimentally established, that increase in the proportion of pumpkin pulp has been found to lead to an increase in mineral content and a decrease in fat content. Improvements in the functional and technological properties of experimental pâté products based on the use of pumpkin pulp have been demonstrated. Established rational replacement level of basic raw materials, which is characteristic for recipes № 3. Pâté products using pumpkin pulp as a functional ingredient is a low-calorie products. The production of combined meat products does not require additional specialized equipment and is a potential source of expansion of the domestic range of functional products. Consumption of these products gives us every reason to believe their food with significant new functional properties that meet the requirements of modern food.

scholarly journals Experience of biotechnology method applications in meat processing industry

2020 ◽  
Vol 17 ◽  
pp. 00197
Author(s):  
Maksim M. Danyliv ◽  
Olga A. Vasilenko ◽  
Olga N. Ozherelyeva ◽  
Ekaterina B. Stanislavskaya
Keyword(s):  
Food Security ◽  
Russian Federation ◽  
Raw Materials ◽  
Meat Products ◽  
Animal Origin ◽  
Low Grade ◽  
Meat Industry ◽  
Meat Processing ◽  
Enzyme Preparations ◽  
The Russian Federation

Nowadays one of the main regulatory documents governing the future of food production in Russia is the “Food Security Doctrine of the Russian Federation” formulated in order to implement the state economic policy in the field of ensuring food security of the Russian Federation, aimed at reliable provision of the country’s population with food products, the development of domestic agricultural and fishery complexes. In the past 40 years, the use of nano- and biotechnologies in the processing of animal and fish raw materials has been widely developed. A large number of scientific studies are aimed at biotechnology method applications in the production of meat products, in particular the use of enzyme proteolytic preparations. The aim of the research presented in this article was to study the dynamics of the hydrolysis of water-, saltand alkali-soluble fractions of low-grade beef and lamb proteins with Megaterin, an enzyme preparation of animal origin. The fulfilled studies allow more precise approach to the use of enzyme preparations for the processing of basic raw materials by the meat industry.

Mini-review: from in vitro to ex vivo studies: an overview of alternative methods for the study of medical biofilms

Biofouling ◽  
2020 ◽  
pp. 1-21
Author(s):  
Débora de Souza Collares Maia Castelo-Branco ◽  
Bruno Rocha Amando ◽  
Crister José Ocadaque ◽  
Lara de Aguiar ◽  
Débora Damásio de Queiroz Paiva ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Alternative Methods

scholarly journals Preparation of phospholipid-based polycarbonate urethanes for potential applications of blood-contacting implants

2020 ◽  
Vol 7 (5) ◽  
pp. 491-504
Author(s):  
Peichuang Li ◽  
Wanhao Cai ◽  
Xin Li ◽  
Kebing Wang ◽  
Lei Zhou ◽  
...  
Keyword(s):  
Microphase Separation ◽  
Blood Compatibility ◽  
Ex Vivo ◽  
Raw Materials ◽  
Direct Synthesis ◽  
Animal Experiments ◽  
Biological Response ◽  
Superior Performance ◽  
Scanning Calorimetry

Abstract Polyurethanes are widely used in interventional devices due to the excellent physicochemical property. However, non-specific adhesion and severe inflammatory response of ordinary polyurethanes may lead to severe complications of intravenous devices. Herein, a novel phospholipid-based polycarbonate urethanes (PCUs) were developed via two-step solution polymerization by direct synthesis based on functional raw materials. Furthermore, PCUs were coated on biomedical metal sheets to construct biomimetic anti-fouling surface. The results of stress–strain curves exhibited excellent tensile properties of PCUs films. Differential scanning calorimetry results indicated that the microphase separation of such PCUs polymers could be well regulated by adjusting the formulation of chain extender, leading to different biological response. In vitro blood compatibility tests including bovine serum albumin adsorption, fibrinogen adsorption and denaturation, platelet adhesion and whole-blood experiment showed superior performance in inhibition non-specific adhesion of PCUs samples. Endothelial cells and smooth muscle cells culture tests further revealed a good anti-cell adhesion ability. Finally, animal experiments including ex vivo blood circulation and subcutaneous inflammation animal experiments indicated a strong ability in anti-thrombosis and histocompatibility. These results high light the strong anti-adhesion property of phospholipid-based PCUs films, which may be applied to the blood-contacting implants such as intravenous catheter or antithrombotic surface in the future.

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