Alkaloid Cytotoxic Test on Ethanol Extract from Itchy Leaves (Laportea decumana (Roxb.) Wedd.)

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Septiani Mangiwa ◽  
Septiani Mangiwa ◽  
Septiani Mangiwa
Keyword(s):  
Cytotoxic Activity ◽  
Total Alkaloid ◽  
Leaf Extract ◽  
Gravimetric Method ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Test Results ◽  
Pain Reliever ◽  
Cytotoxic Test ◽  
Extraction Stage

The Itchy leaf [Laportea decumana (Roxb.) Wedd] is a plant that is widely used as traditional medicine by Papuans as a pain reliever. The purpose of this study was to determine the total level of alkaloids in itchy leaf extract and to test the cytotoxic activity using the BSLT method. The research series began with the extraction stage with the maceration method using 96% ethanol, which tested the alkaloid level. The test was carried out using the gravimetric method and the cytotoxic test using the alkaloid level test results using the BSLT method on shrimp larvae (Artemia salina L). The results showed that itchy leaf extract contained a total alkaloid level of 6.46%. Cytotoxic testing using the BSLT method showed an LC50 of 54.33 ppm and was found to be toxic.

Identification of Secondary Metabolite Compounds and Cytotoxic Activity Test of Papuan Grape (Sararanga Sinuosa Hemsley) Extract using Brine Shrimp Lethality Test (BSLT) Method

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Elsye Gunawan ◽  
Enrick Kharo Etmond ◽  
Linus Yhani Chrystomo
Keyword(s):  
Cytotoxic Activity ◽  
Secondary Metabolite ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Organ System ◽  
Activity Test ◽  
Anti Cancer ◽  
Extraction Stage ◽  
Cancer Agent ◽  
Concentration Series

Papua has a diversity of flora species, one of which is the Papuan Grape (Sararanga sinuosa Hemsley). It is commonly used by the Depapre community, Jayapura, as a stamina booster. This research aims to identify the secondary metabolite compounds, to test the cytotoxic activity of Papuan Grape (Sararanga sinuosa Hemsley) extract, and to determine the best concentration that inhabits the growth of Artemia salina larvae using the BSLT method. This study was conducted with the extraction stage using the maceration method by making use of 96% ethanol solvent. Subsequently, the concentration series 0, 50, 100, 150, 200, 250, 300 ppm of Papuan Grape (Sararanga sinuosa Hemsley) extract were made to test the cytotoxic activity on the mortality of Artemia salina shrimp larvae. The results showed that Alkaloids, Flavonoids, Saponins, and Tannins were compounded as secondary metabolite. An antioxidant research that had been carried out previously had LC50 of green-white fruit (12,49 ± 0,35 mg/ml), orange-red fruit (17,62 ± 3,49 mg/ml) and red fruit (12,23 ± 0,46 mg/ml). The community process one stalk of it into juice and used or consumed it two times a day. An inappropriate dose of traditional medicine usage can affect the organ system and had adverse effects in the future The result of cytotoxic research obtained the value of LC50 in ethanol extract of Papuan Grape was 140,863 ppm, and concentration of 250 ppm was the best concentration to inhibit the growth of shrimp larvae (Artemia salina L). The conclusion of this study was the ethanol extract of Papuan Grape (Sararanga sinuosa Hemsley) showed the highest cytotoxic activity and potentially become an anti-cancer agent.

scholarly journals EFEKTIFITAS EKSTRAK ETANOL DAUN SAWI HIJAU (Brassica rapa Var. Parachinensis) SEBAGAI PELEMBAB KULIT DENGAN SEDIAAN MASKER PEEL-OFF

2019 ◽  
Vol 2 (1) ◽  
pp. 6-15
Author(s):  
Cucu Arum Dwi Cahya ◽  
Aminah Syarifuddin ◽  
Ahmad Syukur Syukur
Keyword(s):  
Experimental Method ◽  
Brassica Rapa ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Moisture Level ◽  
Test Results ◽  
Anova Test ◽  
Skin Moisture

Green mustard plants can be formulater into peel-off masks to moisturize the skin, because based on previous research green mustard leaves cointain flavonoids as antioxidants which can moisturize the skin. The purpose of this study was to find out green mustard leaves can be formulated into peel-off mask preparations and to find out the preparation and to find out the preparation off peel off mask with etanol extract of green mustard leaves can moisturize the skin. This study uses an experimental method. Green mustard leaves are extracted by maceration. In this study, a skin analyzer was usee as a mesure of skin moisture level after using peel off mask from a variety of dosage formulations which were divided into 5 preparations, blank (without mask, 3%,4%,5%), postive control (using peel off masks on the market). The Anova test results show a sig value of 0,000 or p<0,005 so it is concluded that the variabel has a difference. The conclusion of this study proves that the ethanol extract of green mustard leaves can be used as a peel-off mask for skin moisturizer, where the higher the concentration of mustard leaf extract, the higher the moisture vlue of the skin.

scholarly journals UJI FITOKIMIA DAN PENENTUAN Inhibition Concentration 50% PADA BEBERAPA TUMBUHAN OBAT DI PULAU TIDORE

2014 ◽  
Vol 14 (2) ◽  
pp. 95
Author(s):  
Wiwin Abdullah ◽  
Max Revolta J. Runtuwene ◽  
Vanda Selvana Kamu
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Seed Extract ◽  
Test Results ◽  
Active Compounds ◽  
Inhibition Concentration ◽  
Dpph Method ◽  
Ic50 Values

ABSTRAK Penelitian ini dilakukan untuk mengetahui senyawa aktif yang terkandung melalui pengujian fitokimia dan aktivitas antioksidan pada tumbuhan obat di pulau Tidore. Uji aktivitas antioksidan menggunakan metode DPPH dilanjutkan dengan perhitungan Inhibition Concentration 50% (IC50). Hasil yang diperoleh adalah biji buah mojoi terkandung senyawa alkaloid dan saponin, buah coro terkandung alkaloid, flavonoid, dan saponin, pada daun ofo terkandung  alkaloid, tanin, flavonoid, steroid, dan saponin dan pada rimpang kuso mafola terkandung alkaloid, tanin, flavonoid, steroid, dan saponin. Nilai IC50 sebagai berikut biji rimpang kuso mafola 37,30 ppm, buah coro 250,17 ppm, daun ofo 976,10 ppm  dan buah mojoi 1001, 07 ppm. Kata kunci :Tumbuhan  obat, DPPH, antioksidan dan uji fitokimia. PHYTOCHEMICALS TEST AND DETERMINATION Inhibition Concentration 50% ON SOME MEDICINAL PLANTS IN THE TIDORE ISLAND ABSTRACT This study was conducted to determine the active compounds contained in an assessment of phytochemical and antioxidant activity in the medicinal plants of Tidore island. The test antioxidant activity was used DPPH method. In the test results to the phytochemical , that mojoi fruit seeds contained alkaloids and saponins, fruit coro (alkaloids, flavonoids, and saponins), ofo leaves (alkaloids, tannins, flavonoids, steroids, and saponins) and ethanol extract of rhizome kusomafola (alkaloids, tannins, flavonoids, steroids, and saponins. In  calculation of IC50 values ​​for rhizome kusomafola 37.30 ppm, 250.17 ppm coro fruit, 976.10 ppm ofo leaf extract, and fruit seed extract mojoi 1001.07 ppm. Keywords: Medicinal plants, DPPH, antioxidant and phytochemical test.

scholarly journals A Cytotoxic Activity of Tahongai (Kleinhovia hospita Linn.) Leaves Extracts Using Brine Shrimp Lethality Test

2019 ◽  
Vol 4 (3) ◽  
pp. 60
Author(s):  
Indah Solihah ◽  
Herlina Herlina ◽  
Riana Sari Puspita Rasyid ◽  
Tri Suciati ◽  
Khairunnisa Khairunnisa
Keyword(s):  
Statistical Analysis ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Ethanol Extracts

Tahongai (Kleinhovia hospita Linn.) leaves had been known contain alkaloids, flavonoids, saponins, steroids, and tannins. At Komering, South Sumatera tribe, tahongai leaves had been known to treat tumor, cancer, polyps, acne, and dysmenorrhea. The study of cytotoxic activity of tahongai bark and stem were done. This study aims to determine the cytotoxic activity of tahongai leaves extracts using BSLT method. Tahongai leaves were extracted using gradual maceration with n-hexane, ethyl acetate, and ethanol 96%. Each extract was tested cytotoxic activity towards Artemia salina L. larvae. The yield of n-hexane, ethyl acetate, and ethanol extracts are 2,686%, 7,033%, and 7,933% respectively. Ethanol extract of tahongai leaves had the best cytotoxic activity with lethality value 76,667% at 500ppm. Statistical analysis with two way ANOVA showed extract and concentration had a significant (p<0,05) effect on larvae lethality percentage.

scholarly journals Analisis Fitokimia Dan Toksisitas Dari Ekstrak Daun Tumbuhan Mempening (Lithocarpus bancanus (Scheff.) Rehd) Menggunakan Metode BSLT (Brine Shrimp Lethality Test)

2019 ◽  
Vol 2 (2) ◽  
pp. 29-33
Author(s):  
Retno Puji Lestari ◽  
Rudi Hendra Sy ◽  
Hilwan Yuda Teruna
Keyword(s):  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Brine Shrimp Lethality ◽  
The Family ◽  
Tribal Region

Mempening (Lithocarpus bancanus (Scheff.) Rehd) is a species belongs to the family Fagaceae which is found growing around the Talang Mamak tribal region in Kelayang District, Indragiri Hulu Regency, Riau Province. The purpose of this study was to determine the toxicity of leaf extract of L. bancanus (Scheff.) Rehd against Artemia salina Leach larvae using the Brine Shrimp Lethality Test (BSLT) method and its phytochemical analysis. The results of the toxicity was determined against n-hexane, dichloromethane, ethyl acetate, methanol, and ethanol extract and they showed LC50 values ​​of 3.15 ppm; 41163.26 ppm; 5817.09 ppm; 817, 27 ppm; 9019.70 ppm. Phytochemical analysis of the leaves of L. bancanus (Scheff.) Rehd plant showed a class of flavonoids, terpenoids, phenolics and saponins.

Uji Aktivitas Sitotoksik Ekstrak Etanol dari Variasi Teh Daun Sirsak (Annona muricata Linn) Terhadap Larva Udang (Artemia salina Leach)

2016 ◽  
Vol 6 (1) ◽  
pp. 13
Author(s):  
Mega Yulia ◽  
Devahimer Harsep Rosi
Keyword(s):  
Cytotoxic Activity ◽  
Green Tea ◽  
Ethanol Extract ◽  
Black Tea ◽  
Artemia Salina ◽  
Tea Leaves ◽  
Lc50 Value ◽  
Oolong Tea ◽  
Activity Test ◽  
Processing Techniques

A research on the cytotoxic activity of several variants leaves of the soursop (Anona muricata Linn). Soursop leaf is processed into three kinds of tea leaves are green tea, black tea, oolong tea. Cytotoxic activity test was conducted using "Brine Shrimp Lethality Test". LC50 value of the three extracts of the leaves of the soursop is 102.32 ppm for ethanol extract of green tea, black tea 38.01 ppm and 63.09 ppm for oolong tea. Soursop leaf tea processing techniques can affect the cytotoxic activity.

scholarly journals The Effect Of Dosage Of Mangrove Leaf Extract Avicennia Marina On The Viability Of Hela Cells

2021 ◽  
Vol 5 (1) ◽  
pp. 41
Author(s):  
Mahbubur Rahman
Keyword(s):  
Hela Cells ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Avicennia Marina ◽  
Reproductive Organs ◽  
Test Results ◽  
Mangrove Plant ◽  
Papilloma Virus ◽  
Ic50 Value ◽  
Leaf Powder

Cervical cancer is caused by infection with the Human Papilloma Virus (HPV) which attacks the reproductive organs of sexually active women. Treatment is done alternatively using natural materials such as mangrove plants. Avicennia marina is a type of mangrove plant that has been used in alternative medicine because of its potential as an anticancer. This study aimed to determine the effect of Avicennia marina mangrove leaf extract on the viability of HeLa cells. Avicennia marina mangrove leaf powder was extracted using graded maceration. The solvents used include n-hexane, ethyl acetate, and ethanol. The results showed that the LC50 value was 98.55 ppm, it means that the ethanol extract has toxic properties. Phytochemical test results of Avicennia marina mangrove leaf extract contain saponins, steroids/triterpenoids, flavonoids and tannins. The test results showed that the extract yield was 14.40%, the water content of the extract was 16.57%, and the total phenol was 1915.92 mg/g GAE. The results of the LC- MS test resulted in suspected compounds including Caffeine and Diosmetin. The ethanol extract of Avicennia marina mangrove leaves was cytotoxic to heLa cell viability with the resulting IC50 value of 115.345 g/mL.

scholarly journals Efek Sitotoksik Ekstrak Etanolik Ocimum basilicum, L. Pada Sel Kanker Payudara

2019 ◽  
Vol 6 (2) ◽  
pp. 74
Author(s):  
Devi Nisa Hidayati ◽  
Ibrahim Arifin ◽  
Fatimatuz Zahroh ◽  
Lina Wahyuni
Keyword(s):  
Breast Cancer ◽  
Cancer Treatment ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Mtt Assay ◽  
Breast Cancer Cells ◽  
Ethanol Extract ◽  
Ocimum Basilicum ◽  
Cytotoxic Test ◽  
Mcf 7

ABSTRAK Pengobatan kanker menggunakan bahan alam terus dikembangkan. Salah satu tanaman yang memiliki efek sitotoksik Ocimum basilicum, L. tujuan penelitian ini adalah mengetahui aktivitas sitotoksik dari ekstrak etanol Ocimum basilicum (EEOB) terhadap sel kanker payudara T47D dan MCF7. Ocimum basilicum diekstraksi menggunakan metode maserasi dengan pelarut etanol 70%. Pengujian aktivitas sitotoksik menggunakan metode MTT assay dengan  seri konsentrasi EEOB 1000; 500; 250; 125; 62.5; 31,25 µg/mL. Hasil uji aktivitas sitotoksik EEOB memperlihatkan nilai IC50 pada sel T47D dan MCF-7 sebesar 399.86 µg/ml dan 387.76 µg/ml. Kata Kunci—Sitotoksik, Ocinum basilicum L., T47D, MCF-7  ABSTRACT             Cancer treatment using natural ingredients continues to be developed. One of the plants that is proven to have cytotoxic activity is basil leaves (Ocimum basilicum, L.). This study aims to determine the cytotoxic activity of ethanol extract of basil leaves (EEBL) on T47D and MCF-7 breast cancer cells. Basil leaves were extracted using maceration  with ethanol 70%. The cytotoxic test was perfomed using MTT assay with various EEBL concentrations: 1000; 500; 250; 125; 62.5; 31,25 µg/mL. The results showed that IC50 of cytotoxic activity in T47D and MCF-7 was 399.86 µg/ml and 387.76 µg/ml respectively. Keywords—Cytotoxic, Ocinum basilicum L., T47D, MCF-7

scholarly journals Skrining Senyawa Kimia dan Uji Aktivitas Sitotoksik Ekstrak Etanol Teripang Kridou Bintik (Bohadschia argus Jeager) Asal Pantai Harlem Kabupaten Jayapura, Papua

2019 ◽  
Vol 11 (1) ◽  
pp. 12-17
Author(s):  
Apianus Kilungga ◽  
Linus Y. Chrystomo ◽  
Puguh Sujarta
Keyword(s):  
Secondary Metabolites ◽  
Cytotoxic Activity ◽  
Key Words ◽  
Chemical Compound ◽  
Chemotherapeutic Agent ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Artemia Salina ◽  
Chemical Reagents ◽  
Lc50 Value

The Bohadschia argus Jeager contains bioactive compounds that have the potential as antibiotics, antibacterial, antitumor, anticoagulants and anesthetics and alleviate cancer. This study aims to determine the content of chemical compounds group and to test the cytotoxic activity of ethanol extract of Bohadschia argus Jeager. The method for determining the group of chemical compounds was used the color reaction and precipitation using chemical reagents. The method for testing cytotoxic activity using the Brine Shimp Lethality Test (BSLT), this method commonly was used to test the safety of the use of natural ingredients as traditional medicine. The results of screening of the chemical compound group of  Bohadschia argus Jeager extract showed that the extract contained a lot of alkaloids secondary metabolites and few saponins. The results of testing of cytotoxic activity showed LC50 value of 878.22 ppm. Base on the results of this study it can be concluded that the ethanol extract of Bohadschia argus Jeager has significant cytotoxic activity against Artemia salina Leach, so it can be considered as a chemotherapeutic agent.   Key words: Screening, chemical compounds, ethanol extract, B. argus, BSLT. 

UJI TOKSISITAS AKUT EKSTRAK ETANOL AKAR KB (Coptosapelta tomentosa Valeton ex K.Heyne) DENGAN METODE Brine Shrimp Lethality Test (BSLT)

2017 ◽  
Vol 2 (2) ◽  
pp. 161
Author(s):  
Risa Supriningrum ◽  
Sapri Sapri ◽  
Vici Ali Pranamala
Keyword(s):  
Acute Toxicity ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Analysis ◽  
Test Results ◽  
Brine Shrimp Lethality ◽  
Lc50 Value

The roots of KB plants (Captosapelta tomentisa) is used empiricallyby  Dayak Kenyah community as a drug leukorrhea. Leukorrhea is one of the early symptoms of cervical cancer. Early methods to determine the LC50 value or toxicity of anticancer KB roots have not been reported. The aim of research is to determine the value of the toxicity of KB root. Research was conducted by an experimental study. Research stages include sample processing, extraction and acute toxicity test by Brine Shrimp Lethality Test (BSLT). BSLT testing to determine LC50 values with a concentration of 250 ppm, 300 ppm, 400 ppm, 450 ppm and a negative control. Analysis of the data used are probit analysis method. The results showed  the presence of flavonoids, alkaloids and saponins in of the ethanol extract of KB root . The test results obtained acute toxicity LC50 value is 299.226 mg / ml, so that the ethanol extract of roots KB potentially toxic to the larvae of Artemia salina Leach.

Sign in / Sign up

Export Citation Format

Share Document