scholarly journals Influence Analysis of Heating Time Towards Degradation of Anti Oxidant Activity on Water Spinach Leaves (Ipomoea Aquatica Forsk)

2017 ◽  
Vol 2 (1) ◽  
pp. 91
Author(s):  
Akhmad Baihaqi Arsyad ◽  
Ratih Rizqi Nirwana ◽  
R. Arizal Firmansyah
Keyword(s):  
Antioxidant Activity ◽  
Extraction Process ◽  
Heating Time ◽  
Water Spinach ◽  
Time Duration ◽  
Ipomoea Aquatica ◽  
Ic50 Value ◽  
Anti Oxidant ◽  
The Impact ◽  
Spinach Leaves

<div style="text-align: justify;">The purpose of this research is to analyze the impact of water spinach leaves heating (Ipomoea aquatica Forsk) at 100oC by different time duration on its antioxidant activity. The method was experimental method involved several steps, such as sample preparation, extraction process, and evaluation of antioxidant activity with UV-Visible Spectrophotometer. The result of this research shown that IC50 value from K1, K2, K3 to K4 were 25.25 μg/mL (very high), 96.75 μg/mL (high), 181.47μg/L (low, and 280μg/mL (very low). The IC50 value of K1 to K2 decreased 71.49 μg/L, The K2 to K3 decreased 84.72 μg/L, K3 to K4 decreased 98.53 μg/L. By this result, it was predicted that the antioxidant of water spinach will diminish, or even it will be lowered when it is steamed more than 15 minutes in 100 C. ©2016 JNSMR UIN Walisongo. All rights reserved.</div>

scholarly journals Coconut shell derived biochar to enhance water spinach (Ipomoea aquatica Forsk) growth and decrease nitrogen loss under tropical conditions

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Fengliang Zhao ◽  
Ganghua Zou ◽  
Ying Shan ◽  
Zheli Ding ◽  
Minjie Dai ◽  
...  
Keyword(s):  
Production System ◽  
Nitrogen Loss ◽  
Application Rate ◽  
Coconut Shell ◽  
N Leaching ◽  
N Loss ◽  
Water Spinach ◽  
Ipomoea Aquatica ◽  
Tropical Conditions ◽  
The Impact

AbstractFarms usually apply excessive nitrogen (N) fertilizers, especially in a vegetable production system, resulting in severe N leaching loss. Although there have been some reports on the impact of biochar on the N leaching in farmlands, most of them focused on field crops in temperate or subtropical religions. Limited information about N leaching in the tropical vegetable system is available regarding the quantitative data and effective countermeasures. A field experiment was conducted to quantify N leaching in a tropical leafy production system (Ipomoea aquatica Forsk) and to evaluate the effects of coconut shell biochar on N loss and crop growth. The results showed that compared to conventional fertilization with the 240 kg N ha−1 application rate (NPK), biomass yield of water spinach increased by 40.1% under the high biochar application rate of 48 t ha−1 (HBC), which was significantly higher than that of NPK treatment. Moreover, The HBC treatment decreased N leaching by 34.0%, which can be attributed to enhanced crop uptake which increased by 40.3% as compared to NPK treatment. The NH4+/NO3− ratio in leachates was between 0.01 and 0.05. It was concluded that coconut shell derived biochar improved the biomass yields of water spinach and reduced the leaching N loss, which provides a promising amendment in tropical regions.

scholarly journals The Mechanically Extraction Process of Gambier (Uncaria gambier Roxb.) from Limapuluh Kota, West Sumatera and Its Antioxidant activity

2020 ◽  
Vol 9 (1) ◽  
pp. 8-15
Author(s):  
Galuh Widiyarti ◽  
◽  
Andini Sundowo ◽  
Euis Filailla ◽  
Joddy Arya Laksmono ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Trace Amount ◽  
Extraction Process ◽  
Activity Analysis ◽  
Hydraulic Press ◽  
Screw Press ◽  
Ic50 Value ◽  
Dpph Method ◽  
Twin Screw ◽  
Water Contents

The extraction process from leaves and twigs of gambier (Uncaria gambier Roxb)plant was conducted mechanically by using traditional hydraulic press, conventional screw press, and modified twin-screw press. The leaves and twigs of gambier plant was obtained from traditional farmer in Limapuluh Kota, West Sumatera, Indonesia. The water, ash and catechin contents of the gambier extracts were analyzed based on SNI 01-3391-2000 using spectrophotometry and thermogravimetry method. Antioxidant activity analysis of the extracts was also performed by 1,1-diphenyl-2-picrylhidrazyl (DPPH) method and compared tovitamin C as a standard antioxidant. The analysis results showed that the extracts contain no ash. Meanwhile, the catechin and water contents of the extracts were approximately 50 and 13% thus the extracts were classified as quality gambier 2. Other than that, analysis result of catechin and epicatechin contents of the extracts using HPLC and compared to the reference materials showed that catechins contents of the extracts using traditional hydraulic press, conventionalscrew press, and modified twin-screw press give catechin content in about 94.296-95.030%. However, epicatechin was detected in a trace amount. The antioxidant activity of the extracts were 2.5 times stronger than reference. The IC50 value of 4.37-4.52 µg/mL and was categorized as active antioxidant.

scholarly journals Antioxidant activity of Indonesian water spinach and land spinach (Ipomoea aquatica): A comparative study

2019 ◽  
Vol 1402 ◽  
pp. 055091
Author(s):  
R Mariani ◽  
F Perdana ◽  
F M Fadhlillah ◽  
A Qowiyyah ◽  
H Triyana
Keyword(s):  
Antioxidant Activity ◽  
Comparative Study ◽  
Water Spinach ◽  
Ipomoea Aquatica

scholarly journals Penentuan Kadar Flavonoid Total dan Uji Antioksidan Ekstrak Etanol Daun Kasturi (Mangifera casturi Kosterm.) dengan Metode DPPH

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Alifni Adha Bakti ◽  
Liling Triyasmono ◽  
Muhammad Ikhwan Rizki
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Natural Resources ◽  
Experimental Research ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Ic50 Value ◽  
Anti Oxidant

ABSTRAK Bahan alam dapat dijadikan bahan obat baru karena mengadung metabolit sekunder. Di Indonesia terdapat lebih kurang 30.000 jenis tumbuh-tumbuhan yang mengandung metabolit sekunder, lebih kurang 7.500 jenis diantaranya termasuk tanaman berkhasiat obat. Salah satunya adalah tanaman kasturi (Mangifera casturi Kosterm.). Tanaman M. casturi merupakan tumbuhan khas Kalimantan Selatan yang hanya dimanfaatkan oleh masyarakat untuk dikonsumsi, tidak untuk pengobatan. Penelitian ini bertujuan untuk menentukan kadar flavonoid total dan aktivitas antioksidan dari ekstrak etanol daun M. casturi. Penelitian ini bersifat non-eksperimental. Sampel yang digunakan adalah daun M. casturi yang berasal dari Kabupaten Banjar, Kalimantan Selatan. Penentuan Kadar flavonoid total dilakukan secara spektrofotometri UV-Vis dengan pereaksi kompleks AlCl3 sedangkan aktivitas antioksidan ditentukan dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Hasil penelitian diperoleh kadar flavonoid total ekstrak etanol daun M. casturi sebesar 9,31 ± 0,08 %b/b dan aktivitas antioksidan dengan nilai IC50 sebesar 34,558 ppm sehingga termasuk dalam kategori antioksidan yang sangat aktif. Kata kunci: Antioksidan, DPPH, Flavonoid Total, Mangifera casturi. ABSTRACT Natural resources can be used as the new medicine ingridients because it has second metabolite. In Indonesia, there are more than 30.000 kinds of plants that contain second metabolite, more or less than 7.500 kinds of those are medicinal plants. One of those plants is Kasturi (Mangifera casturi Kosterm.). M. casturi is a typical plant from South Kalimantan that be used as a food not as a medicine. The purpose of this research is to determine the total of flavonoid content and anti-oxidant activity from ethanol extract of M. casturi leaves. This study is a non-experimental research. Sample which used in this research is M. casturi Leaves from Banjar Region, South Kalimantan. The research for total Flavonoid content is done by UV-Vis spectrophotometric with AlCl3 reagent complex while the anti-oxidant activity is determined by DPPH (2,2-difenil-1-pikrilhidrazil) method. The result of this research are extract of M. casturi leaves obtains 9,31 ± 0,08 %b/b of total flavonoid and the antioxidant activity result with IC50 value is 34,558 ppm, so it can be categorized as a very active anti-oxidant. Keywords: Antioxidant, DPPH, Total Flavonoid, Mangifera casturi.

scholarly journals Antioxidant Activities of Muntingia calabura, Syzygium cumini, Ocimum basilicum, and Eleutherine bulbosa using DPPH Method

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Ani Haerani ◽  
Anis Yohana Chaerunisa ◽  
Anas Subarnas
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Extraction Process ◽  
Ocimum Basilicum ◽  
Syzygium Cumini ◽  
Determination Process ◽  
Ic50 Value ◽  
Dpph Method ◽  
Muntingia Calabura

Antioxidants are substances that can provide endogenous protection and exogenous oxidative stress by capturing free radicals. Many plants are efficacious as antioxidants, namely plants that contain polyphenols, especially flavonoids, so many are formulated as natural antioxidants. Plants such as Muntingia calabura, Syzygium cumini, Ocimum basilicum, and Eleutherine bulbosa contain polyphenol compounds, especially flavonoids which are efficacious as natural antioxidants. This research aimed to study antioxidant activity derived from some potential plants using the DPPH method by calculating the IC50 value of each plant extract. This research method starts from the determination process to prove the validity of the plants used, the extraction process using the maceration method with 70% ethanol solvent, then the antioxidant activity of extracts from each plant was carried out using the DPPH method. This research starts from the determination process to ensure the correctness of the plants used, then the extraction process is carried out using the maceration method with 70% ethanol solvent. After that the antioxidant activity was determined from the four plants using the DPPH method to see the strongest IC50 value among the four plants. IC50 is the concentration of the sample to inhibit 50% of free radicals. The results of IC50 values from ethanol extract of M. calabura leaves, Syzygium cumini leaves, Ocimum basilicum leaves and Eleutherine bulbosa bulbs, were 18.72; 63,84; 141.59 and 173.15 ppm. Ethanol extract of M. Calabura has a smaller IC50 value of 18.72 ppm which has a very strong and most powerful antioxidant from the ethanol extract of Syzygium cumini, Ocimum basilicum and Eleutherine bulbosa. Keywords : Antioxidant, Muntingia calabura, Syzygium cumini, Ocimum basilicum, Eleutherine bulbosa, DPPH Method

scholarly journals Effects of Temperature and Heating Time on Degradation and Antioxidant Activity of Anthocyanin from Roselle Petals (Hibiscus sabdariffa L.)

2020 ◽  
Vol 1 (4) ◽  
pp. 288-238
Author(s):  
Meiny Suzery ◽  
Burhan Nudin ◽  
Damar Nurwahyu Bima ◽  
Bambang Cahyono
Keyword(s):  
Antioxidant Activity ◽  
Heating Time ◽  
Heating Process ◽  
Anthocyanin Content ◽  
Hibiscus Sabdariffa ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Ic50 Value ◽  
Dpph Method ◽  
Effects Of Temperature

The effects of temperature and heating time on the degradation and antioxidant activity of anthocyanin compounds from roselle petals (Hibiscus sabdariffa L.) have been evaluated from this research. The purpose of this study is to determine, the degradation and antioxidant activity of anthocyanin compounds on variations of temperature and heating time. In this study anthocyanin was extracted from roselle petals using ethanol solvent at 25oC. Then the anthocyanin extract obtained was dissolved into aqueous solution. The solution was then heated at 40oC, 60oC and 80oC for 60 minutes. The degradation of anthocyanin compounds was analyzed by calculating the total of anthocyanin contents, while the antioxidant activity was evaluated using the DPPH method. The results showed that Total Anthocyanin Content (TAC) from the extract solution before heating was 144.28 mg/L. Meanwhile the total value of anthocyanin in the 60 minutes after heating at 400C, 600C and 800C are 108.88 mg/L, 88.84 mg/L and 63.78 mg/L respectively. Heating at 80oC decreases antioxidant activity by increasing the IC50 value to 86.8 ppm higher than the conditions before heating. The increase of temperature and length of the heating process made the anthocyanin structure degraded. Degradation was indicated by the reduction of total anthocyanin content and the decrease of antioxidant activity.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK BAWANG HITAM (BLACK GARLIC) DENGAN VARIASI LAMA PEMANASAN

2020 ◽  
Vol 13 (1) ◽  
pp. 39-50
Author(s):  
Eva Agustina ◽  
Funsu Andiarna ◽  
Irul Hidayati
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Heating Time ◽  
The Body ◽  
Garlic Extract ◽  
Active Compounds ◽  
Rotary Evaporator ◽  
Ic50 Value ◽  
Ic50 Values ◽  
The Difference

AbstrakSenyawa radikal dalam tubuh sangat reaktif terhadap sel-sel, sehingga dapat menyebabkan berbagai penyakit. Senyawa yang mampu menangkal radikal bebas disebut antioksidan. Bawang hitam diketahui mengandung senyawa aktif fenolik dan turunannya sehingga dapat digunakan sebagai antioksidan. Penelitian tentang penggunaan bawang hitam sebagai antioksidan ini bertujuan untuk mengetahui perbedaan waktu pemanasan (15, 25, dan 35 hari) terhadap aktivitas antioksidan pada ekstrak bawang hitam. Bawang hitam diperoleh dengan cara memanaskan bawang putih selama 15, 25, dan 35 hari. Bawang hitam selanjutnya diekstraksi dengan pelarut metanol dengan metode maserasi. Filtrat hasil ekstraksi dipekatkan dengan rotary evaporator untuk mendapatkan ekstrak bawang hitam. Ekstrak bawang hitam diuji fitokimia untuk mengetahui kandungan senyawa aktif. Aktivitas antioksidan ekstrak bawang hitam ditentukan dengan metode penangkalan radikal 2,2-diphenyl-1-picrylhydrazyl (DPPH). Hasilnya menunjukkan ekstrak bawang hitam memiliki aktivitas antioksidan yang sangat kuat dengan nilai IC50 masing-masing 15 hari yaitu 2,41 µg/mL; 25 hari yaitu 2,93 µg/mL; 35 hari yaitu 2,27 µg/mL. Nilai IC50 <10 μg/mL menunjukkan bahwa ekstrak bawang hitam dengan waktu pemanasan 15, 25, dan 35 hari memiliki potensi antioksidan yang sangat kuat. Waktu pemanasan yang paling optimum dalam penangkalan radikal bebas adalah ekstrak bawang hitam dalam waktu pemanasan 35 hari, karena banyak terdapat kandungan senyawa flavonoid, tanin, sterol, dan saponin.Abstract Radical compounds in the body are very reactive to cells that can cause various diseases. Compounds that are able to ward off free radicals are called antioxidants. Black garlic are known to contain phenolic active compounds and their derivatives so that they can be used as antioxidants. Research on the use of black garlic as an antioxidant aims to determine the difference in heating time (15, 25, and 35 days) on the antioxidant activity of black garlic extract. Black garlic are obtained by heating the garlic for 15, 25, and 35 days. The black garlic is then extracted with methanol solvent by maceration method. The extracted filtrate was concentrated with a rotary evaporator to obtain the black garlic extract. Black garlic extract was tested for phytochemistry to determine the content of active compounds. The antioxidant activity of black garlic extract was determined by the radical deterrence method 2,2-diphenyl-1-picrylhydrazyl (DPPH). The results showed that the onion extract had a very strong antioxidant activity with an IC50 value of 15 days each of 2.41 µg/mL; 25 days which is 2.93 μg/mL; 35 days which is 2.27 µg/mL. IC50 values <10 μg/mL indicate that the black garlic extract with a heating time of 15, 25, and 35 days has a very strong antioxidant potential. The most optimum heating time in deterring free radicals is black garlic extract within 35 days of heating because there are many compounds containing flavonoids, tannins, sterols, and saponins.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Daun Biwa (Eriobotrya japonica (Thunb.) Lindl.) Terhadap DPPH (1,1-diphenyl-2-picrylhydrazyl)

2018 ◽  
Vol 1 (3) ◽  
pp. 086-089
Author(s):  
Marianne Marianne ◽  
Revi Septiani ◽  
Yuliana Yuliana
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Eriobotrya Japonica ◽  
Ic50 Value ◽  
Anti Oxidant ◽  
North Sumatra

Biwa (Eriobotrya japonica (Thunb.) Lindl.) dapat tumbuh dengan mudah di dataran tinggi di Sumatera Utara. Tanaman ini memiliki banyak khasiat dalam mengobati berbagai penyakit. Penelitian ini bertujuan untuk mengevaluasi aktivitas antioksidan daun E. japonica dengan menggunakan DPPH (1,1-difenil-2-pikrilhidrazil). Daun E. japonica dimaserasi menggunakan etanol 96%. Ekstrak diuji dengan metode pemerangkapan radikal bebas menggunakan DPPH. Kuersetin digunakan sebagai antioksidan standar. Ekstrak diukur pada konsentrasi 0, 20, 40, 60 dan 80 ppm dengan menggunakan spektrofotometer pada panjang gelombang 516 nm, setelah diinkubasi 15 menit dengan DPPH. Daun E. japonica memiliki nilai IC50 56,59 µg/mL sedangkan IC50 kuersetin adalah 4,36 µg/mL. Sehubungan dengan itu, daun E. japonica digolongkan sebagai antioksidan kuat dan berpotensi untuk dikembangkan lebih lanjut.   Biwa (Eriobotrya japonica (Thunb.) Lindl.) can grow easily in the highlands in North Sumatra. This plant can be used to treat various diseases. This research aimed to evaluate the antioxidant activity of E. japonica leaves using DPPH (1,1-diphenyl-2-pikrilhidrazil). E. japonica leaves were macerated using ethanol 96%. The extract was evaluated by free radical scavenging method using DPPH. Quercetin was used as standard antioxidant.  The extract was measured with concentrations of 0, 20, 40, 60 and 80 ppm using spectrophotometer at 516 nm wavelength, after incubated with DPPH for 15 minutes. E. japonica leaves showed the IC50 value of 56,59 µg/mL, meanwhile, the IC50value of quercetin was 4,36 µg/mL. According to the result, E. japonica leaf was categorized as strong anti-oxidant and can be further developed

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