Antioxidant Activities of Muntingia calabura, Syzygium cumini, Ocimum basilicum, and Eleutherine bulbosa  using DPPH Method

Antioxidants are substances that can provide endogenous protection and exogenous oxidative stress by capturing free radicals. Many plants are efficacious as antioxidants, namely plants that contain polyphenols, especially flavonoids, so many are formulated as natural antioxidants. Plants such as Muntingia calabura, Syzygium cumini, Ocimum basilicum, and Eleutherine bulbosa contain polyphenol compounds, especially flavonoids which are efficacious as natural antioxidants. This research aimed to study antioxidant activity derived from some potential plants using the DPPH method by calculating the IC50 value of each plant extract. This research method starts from the determination process to prove the validity of the plants used, the extraction process using the maceration method with 70% ethanol solvent, then the antioxidant activity of extracts from each plant was carried out using the DPPH method. This research starts from the determination process to ensure the correctness of the plants used, then the extraction process is carried out using the maceration method with 70% ethanol solvent. After that the antioxidant activity was determined from the four plants using the DPPH method to see the strongest IC50 value among the four plants. IC50 is the concentration of the sample to inhibit 50% of free radicals. The results of IC50 values from ethanol extract of M. calabura leaves, Syzygium cumini leaves, Ocimum basilicum leaves and Eleutherine bulbosa bulbs, were 18.72; 63,84; 141.59 and 173.15 ppm. Ethanol extract of M. Calabura has a smaller IC50 value of 18.72 ppm which has a very strong and most powerful antioxidant from the ethanol extract of Syzygium cumini, Ocimum basilicum and Eleutherine bulbosa. Keywords : Antioxidant, Muntingia calabura, Syzygium cumini, Ocimum basilicum, Eleutherine bulbosa, DPPH Method

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SKRINING FITOKIMIA, UJI AKTIVITAS ANTIOKSIDAN, DAN TOKSISITAS DARI EKSTRAK DAUN KERSEN (Muntingia calabura L.) DENGAN METODE 1.1-diphenyl-2-picrylhydrazyl (DPPH) dan Brine Shrimp Lethality Test (BSLT)

PHARMACON ◽

10.35799/pha.8.2019.29297 ◽

2019 ◽

Vol 8 (2) ◽

pp. 315

Author(s):

Selin Widjaya ◽

Widdhi Bodhi ◽

Adithya Yudistira

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Brine Shrimp ◽

Ethanol Extract ◽

Brine Shrimp Lethality ◽

Phytochemical Content ◽

Lc50 Value ◽

Ic50 Value ◽

Dpph Method ◽

Muntingia Calabura

ABSTRACTKersen (Muntingia calabura L.) is a plant that has begun to be eliminated and was rarely used because it is often considered to have no economic value and lack of knowledge about its utilization, whereas kersen plants contain flavonoids, saponins, and tannins which were have high benefit for health. The content of metabolites is affected by soil nutrient elements and difference place of growth. This study aims to determine the potential of kersen leaves grown in North Minahasa based on phytochemical content, ability of antioxidant activity, and toxicity. Kersen leaves were extracted using sequential maceration method with n-hexane, ethyl acetate, and ethanol as solvents. Phytochemical Screening using several reagents which tailored to the type of phytochemical test. 1.1-diphenyl-2-picrylhydrazyl (DPPH) method is used to evaluate antioxidant activity, and Brine Shrimp Lethality Test (BSLT) method is used to evaluate toxicity. The result of this study indicate that the n-hexane extract contains phenols, flavonoids, and tannins, with IC50 value 12.54 μg/mL, and LC50 value 881 μg/mL. Ethyl acetate extract contains phenols, flavonoids, tannins, and saponins, with IC50 value 61.3 μg/mL, and LC50 value 1758 μg/mL. Ethanol extract has phenol, flavonoid, tannin, saponin, and terpenoid content, with IC50 value 9.01 μg/mL, and LC50 value 106 μg/mL. Keywords : Kersen leaves, Antioxidant, Toxicity, IC50, LC50 ABSTRAKKersen (Muntingia calabura L.) merupakan tanaman yang sudah mulai tersingkirkan dan jarang dimanfaatkan karena sering dianggap tidak punya nilai ekonomis dan kurangnya pengetahuan tentang pemanfaatannya, padahal tanaman kersen memiliki kandungan flavonoid, saponin, dan tanin yang bermanfaat tinggi untuk kesehatan. Kandungan senyawa metabolit dipengaruhi oleh unsur hara tanah dan perbedaan tempat tumbuh. Penelitian ini bertujuan untuk mengetahui potensi dari daun kersen yang tumbuh di Minahasa Utara berdasarkan kandungan fitokimia, kemampuan aktivitas antioksidan, dan toksisitasnya. Ekstrak daun kersen diekstraksi dengan metode maserasi sekuensial menggunakan pelarut n-heksan, etil asetat, dan etanol. Skrining fitokimia menggunakan beberapa reagen yang disesuaikan dengan jenis uji fitokimia. Metode 1.1-diphenyl-2-picrylhydrazyl (DPPH) digunakan untuk mengevaluasi aktivitas antioksidan, dan metode Brine Shrimp Lethality Test (BSLT) digunakan untuk mengevaluasi toksisitas. Hasil penelitian ini menunjukkan bahwa ekstrak n-heksan memiliki kandungan fenol, flavonoid, dan tanin, nilai IC50 12,54 μg/mL, dan nilai LC50 881 μg/mL. Ekstrak etil asetat memiliki kandungan fenol, flavonoid, tanin, dan saponin, nilai IC50 61,3 μg/mL, dan nilai LC50 1758 μg/mL. Ekstrak etanol memiliki kandungan fenol, flavonoid, tanin, saponin, dan terpenoid, nilai IC50 9,01 μg/mL, dan nilai LC50 106 μg/mL. Kata kunci : Daun Kersen, Antioksidan, Toksisitas, IC50, LC50

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Aktivitas Antioksidan Fraksi Dietileter Buah Mangga Arumanis (Mangifera indica L.) dengan Metode DPPH

Jurnal Pharmascience ◽

10.20527/jps.v4i1.5760 ◽

2017 ◽

Vol 4 (1) ◽

Author(s):

Dewi Andini Kunti Mulangsri ◽

Aqnes Budiarti ◽

Endah Novia Saputri

Keyword(s):

Antioxidant Activity ◽

Vitamin C ◽

Diethyl Ether ◽

Mangifera Indica ◽

Ethanol Extract ◽

Natural Antioxidants ◽

Antioxidant Systems ◽

Ic50 Value ◽

Dpph Method ◽

Ether Fraction

ABSTRAK Sistem antioksidan merupakan bagian dari sistem kekebalan tubuh yang mampu menghambat reaktifitas radikal bebas. Sumber alami antioksidan salah satunya adalah buah yaitu buah mangga Arumanis. Proses fraksinasi dengan pelarut dietileter dilakukan untuk menyari senyawa-senyawa yang bersifat semipolar yang diharapkan memiliki potensi sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan fraksi dietil eter ekstrak etanol buah mangga arumanis (Mangifera indica L.) dengan Metode DPPH. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 70%, kemudian dipartisi cair-cair menggunakan pelarut dietil eter. Penentuan aktivitas antioksidan menggunakan metode DPPH dengan membuat seri konsentrasi fraksi dietil eter ekstrak etanol buah mangga Arumanis yaitu 12,5; 25; 50 dan 100ppm. Vitamin C digunakan sebagai larutan pembanding dengan seri konsentrasi 0,625; 1,25; 2,5 dan 5 ppm. Hasil penelitian menunjukkan bahwa fraksi dietil eter ekstrak etanol buah mangga arumanis memiliki aktivitas antioksidan dengan nilai IC50 sebesar 75,22 ppm dan 1,18 ppm untuk Vitamin C. Fraksi dietileter ekstrak etanol buah mangga Arumanis memiliki potensi lemah sebagai antioksidan jika dibandingkan dengan Vitamin C. Kata Kunci: antioksidan, fraksi dietileter, buah mangga arumanis, Mangifera indica, DPPH ABSTRACT Antioxidant systems are part of the immune system which is able to inhibit the reactivity of free radical. Source of natural antioxidants is a fruit that is mango Arumanis. Process of fractionation with solvent diethylether to extraction the compounds semipolar and expected to have potential as antioxidants. This study aims to determine the antioxidant activity of diethyl ether fraction of ethanol extract Arumanis mango (Mangifera indica L.) with DPPH method. Extraction used ethanol 70% by maceration method, then partitioned using a liquid-liquid partition with diethylether as a solvent. Determination of antioxidant activity using DPPH method with a series of concentrations of diethyl ether fraction of ethanol extract Arumanis mango ie 12.5; 25; 50 and 100ppm. Vitamin C used as a reference solution with a concentration series of 0,625; 1.25; 2.5 and 5 ppm. The results showed that the diethyl ether fraction of ethanol extract Arumanis mango have antioxidant activity with IC50 value of 7.22 ppm and 1.18 ppm for Vitamin C. Diethylether fraction of ethanol extract Arumanis mango has weak potential as a antioxidant, if compared with Vitamin C. Keywords: Antioxidants, diethylether fractions, arumanis mango fruit, Mangifera indica, DPPH.

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Aktivitas Antioksidan Ekstrak Etanol Muntingia calabura dengan Variasi Laju Pengadukan Menggunakan Macerator-Magnetic Stirrer (M-MS)

Jurnal Pharmascience ◽

10.20527/jps.v7i2.9037 ◽

2020 ◽

Vol 7 (2) ◽

pp. 27

Author(s):

Muhammad Zaini ◽

Hajrah Hidriya ◽

Japeri Japeri

Keyword(s):

Antioxidant Activity ◽

Ethanol Extract ◽

Natural Compounds ◽

Extraction Methods ◽

Magnetic Stirrer ◽

Stirring Rate ◽

Yield Value ◽

Dpph Method ◽

Chemical Content ◽

Muntingia Calabura

Maserasi merupakan salah satu metode ekstraksi yang digunakan untuk menarik kandungan kimia dari bahan alam. Macerator Magnetic Stirrer (M-MS) merupakan alat yang dikembangkan untuk memaksimalkan proses maserasi melalui pengadukan secara berkesinambungan. Tujuan penelitian ini adalah untuk mengetahui efektivitas penggunaan M-MS dalam proses ekstraksi senyawa bahan alam. Parameter yang digunakan yaitu persentase zat terekstraksi (% rendemen) dan aktivitas antioksidan (IC50) ekstrak etanol Muntingia calabura (EEMC) menggunakan metode DPPH (2,2-Diphenyl-1-Picrylhydrazil). Pengujian dilakukan terhadap simplisia daun Muntingia calabura yang dimaserasi selama 24 jam menggunakan etanol 95% dengan maserator konvensional, M-MS dengan laju pengadukan 200 rpm dan 300 rpm. Pengadukan dengan maserator konvensional dilakukan sebanyak 3 kali setiap 8 jam, sedangkan dengan M-MS dilakukan secara kontinyu selama 24 jam. Nilai % rendemen EEMC dari maserator konevnsional, M-MS 200 rpm dan 300 rpm secara berturut-turut adalah 7,6 %, 8,4 % dan 10,2 %. Hasil uji aktivitas antioksidan menghasilkan nilai IC50 EEMC dengan maserator konvensional sebesar 18,19 ppm, M-MS 200 rpm adalah 14,35 ppm dan 300 rpm adalah 7,85 ppm. Nilai IC50 untuk masing-masing uji menunjukan < 50 ppm yang termasuk dalam kategori sangat kuat. Ekstraksi EEMC paling efektif adalah menggunakan M-MS dengan laju pengadukan 300 rpm. The maceration is one of the extraction methods used to extract chemical content from natural compounds. Macerator Magnetic Stirrer (M-MS) is a tool developed to maximize the maceration process through continuous stirring. The purpose of this study was to determine the effectiveness of using M-MS in the process of extracting natural compounds. The parameters used were the percentage of extracted substance (% yield) and antioxidant activity (IC50) of ethanol extract of Muntingia calabura (EEMC) using the DPPH method (2,2-Diphenyl-1-Picrylhydrazil). Tests were carried out on the simplicia of Muntingia calabura leaves which were macerated for 24 hours using 95% ethanol with a conventional macerator, M-MS with a stirring rate of 200 rpm and 300 rpm. Stirring with a conventional macerator was carried out 3 times every 8 hours, while with M-MS it was done continuously for 24 hours. EEMC yield value respectively of conventional macerator, M-MS at 200 rpm and 300 rpm showed 7.6 %, 8.4% and 10.2 %. The results of the antioxidant activity test resulted in the IC50 EEMC value with a conventional macerator is 18.19 ppm, M-MS 200 rpm is 14.35 ppm and 300 rpm is 7.85 ppm. The IC50 value for each test shows <50 ppm which is included in the very strong category. The most effective EEMC extraction is using M-MS with a stirring rate of 300 rpm.Keywords: maceration, M-MS, EEMC, antioxidant

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Aktivitas Antioksidan Ekstrak Etanol Daun Ganitri (Elaeocarpus Ganitrus Roxb.) dengan Metode DPPH (2,2 Difenil-1-Pikrilhidazil)

Pharmacon Jurnal Farmasi Indonesia ◽

10.23917/pharmacon.v18i01.12161 ◽

2021 ◽

Vol 18 (1) ◽

pp. 60-67

Author(s):

Naelaz Zukhruf Wakhidatul Kiromah ◽

Sadam Husein ◽

Titi Pudji Rahayu

Keyword(s):

Antioxidant Activity ◽

Free Radicals ◽

Leaf Extract ◽

Ethanol Extract ◽

Antioxidant Compounds ◽

Synthetic Antioxidant ◽

Activity Test ◽

Impaired Liver ◽

Ic50 Value ◽

Dpph Method

Free radicals are one of the cause of various diseases. The use of synthetic antioxidant compounds could prevent the effect of the free radicals, however may cause adverse effects on the human body such as impaired liver, lung, intestinal and poisoning. Therefore antioxidant from natural resources needs to be developed. The purpose of this research was to determine the antioxidant activity and IC50 value of the ethanol extract of ganitri (Elaeocarpus ganitrus Roxbs.) leaves. Ganitri leaf ethanol extract activity test was carried out using DPPH method with vitamin C as a standard. Antioxidant activity was determined as a decreas in the absorbance of DPPH at 517 nm wavelength after an addition of the extract with the concentrations of 20, 40, 80, and 100 ppm. The antioxidant acitivity measurement of the ganitri leaf extract showed that the linier regression equation obtained was y = 0.3669x + 29.546, r = 0.4573 while the IC50 value was 54,12 ppm. Based on the result, it is concluded that the ethanol extract of ganitri (Elaeocarpus ganitrus Roxb.) leaf showed was categorized as strong antioxidant.

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THE QUALITY PARAMETERS, TOTAL FLAVONOIDS DETERMINATION AND ANTIOXIDANT ACTIVITY COMPOUND OF ANDALIMAN FRUIT ANDALIMAN FRUIT (ZANTHOXYLUM ACANTHOPODIUM DC.) EXTRACT

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2021.v13s2.07 ◽

2021 ◽

pp. 34-40

Author(s):

YUNAHARA FARIDA ◽

WIDI AZELA ◽

MEGA ELOK LESTAR ◽

DIAH KARTIKA PRATAMI

Keyword(s):

Antioxidant Activity ◽

Methyl Palmitate ◽

Ethanol Extract ◽

Quality Parameters ◽

Total Flavonoid ◽

Gas Chromatography Mass Spectrometry ◽

Uv Spectrophotometry ◽

Benzenedicarboxylic Acid ◽

Ic50 Value ◽

Dpph Method

Objective: The aim of this study was to investigate the chemical compound of the antioxidant activity of the extract from andaliman fruit (Zanthoxylum acanthopodium DC.) and the quality parameters and the total flavonoid determination of the highest antioxidant activity. Methods: The andaliman fruits were extracted using a solvent such as n-hexane, ethyl acetate and 70% ethanol obtained extracts, follow the antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The quality parameters and total flavonoid determination were performed from highest antioxidant activity. Fractionated was done using vacuum liquid chromatography (VLC) with the mobile phase chloroform-methanol (9:1 ~1:9). Purification with Thin Layer Chromatography (TLC) preparative, identification using UV-spectrophotometry, FTIR, Gas chromatography Mass Spectrometry (GCMS) analysis. Results: The 70% of ethanol has the highest antioxidant activity with IC50 value 84.11±0.47 ppm; the quality parameters in this study meet the quality requirements, both specific and nonspecific parameters. Total flavonoid content of the 70% ethanol extract was 2.37±0.02%. The results of the GCMS studies showed the presence of 1,2-benzenedicarboxylic acid, mono (2-ethylhexyl) ester; squalene, methyl palmitate, methyl isopalmitate, n-tetracosanol-1 and 4-isopropyl chalcone. Conclusion: The results indicate that the 70% ethanol extracts of andaliman fruits contains 1,2-benzenedicarboxylic acid, mono (2-ethylhexyl) ester; squalene, methyl palmitate, methyl isopalmitate, n-tetracosanol-1 and 4-isopropyl chalcone have antioxidant activity with IC50 value 51.35 ppm.

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The Mechanically Extraction Process of Gambier (Uncaria gambier Roxb.) from Limapuluh Kota, West Sumatera and Its Antioxidant activity

The Journal of Pure and Applied Chemistry Research ◽

10.21776/ub.jpacr.2020.009.01.509 ◽

2020 ◽

Vol 9 (1) ◽

pp. 8-15

Author(s):

Galuh Widiyarti ◽

◽

Andini Sundowo ◽

Euis Filailla ◽

Joddy Arya Laksmono ◽

...

Keyword(s):

Antioxidant Activity ◽

Trace Amount ◽

Extraction Process ◽

Activity Analysis ◽

Hydraulic Press ◽

Screw Press ◽

Ic50 Value ◽

Dpph Method ◽

Twin Screw ◽

Water Contents

The extraction process from leaves and twigs of gambier (Uncaria gambier Roxb)plant was conducted mechanically by using traditional hydraulic press, conventional screw press, and modified twin-screw press. The leaves and twigs of gambier plant was obtained from traditional farmer in Limapuluh Kota, West Sumatera, Indonesia. The water, ash and catechin contents of the gambier extracts were analyzed based on SNI 01-3391-2000 using spectrophotometry and thermogravimetry method. Antioxidant activity analysis of the extracts was also performed by 1,1-diphenyl-2-picrylhidrazyl (DPPH) method and compared tovitamin C as a standard antioxidant. The analysis results showed that the extracts contain no ash. Meanwhile, the catechin and water contents of the extracts were approximately 50 and 13% thus the extracts were classified as quality gambier 2. Other than that, analysis result of catechin and epicatechin contents of the extracts using HPLC and compared to the reference materials showed that catechins contents of the extracts using traditional hydraulic press, conventionalscrew press, and modified twin-screw press give catechin content in about 94.296-95.030%. However, epicatechin was detected in a trace amount. The antioxidant activity of the extracts were 2.5 times stronger than reference. The IC50 value of 4.37-4.52 µg/mL and was categorized as active antioxidant.

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Aktivitas Antioksidan Akar Bawang Merah Lokal Palu (Allium cepa Var Aggergatum L.) dengan Berbagai Kepolaran Pelarut

KOVALEN ◽

10.22487/kovalen.2020.v6.i3.13662 ◽

2020 ◽

Vol 6 (3) ◽

pp. 198-205

Author(s):

Nur Faidah ◽

Nurhaeni ◽

Ahmad Ridhay ◽

Jusman ◽

Abd. Rahman Razak ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Allium Cepa ◽

Ethyl Acetate Extract ◽

Ethanol Extract ◽

Root Extract ◽

Polar Solvents ◽

Ic50 Value ◽

Dpph Method ◽

Keywords Antioxidants

Research on the antioxidant activity of the local onion roots of Palu (Allium cepa Var Aggregatum L.) with various polarity solvents has been carried out. This study aims to determine the potential of Palu local shallot root extract as an antioxidant and determine the IC50 value of the Palu local shallot root antioxidant activity. The method applied in this research is maceration using n-hexane, ethyl acetate, and ethanol. The extracts from each solvent were tested for their antioxidant activity by the DPPH method. The results of the study showed that the IC50 value in n-hexane extract, ethyl acetate extract, and ethanol extract were 579.98 ppm, 518.28 ppm 315.83 ppm and as a comparison used ascorbic acid obtained IC50 of 53.69 ppm. Based on the results obtained, the best antioxidant activity is in polar solvents (ethanol extract) compared to other solvents. Keywords: Antioxidants, roots, Palu local shallots, Allium cepa Var Aggregatum L.

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Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method

Jurnal Pendidikan Kimia ◽

10.24114/jpkim.v13i2.26984 ◽

2021 ◽

Vol 13 (2) ◽

pp. 137-142

Author(s):

Kasta Gurning ◽

◽

Sifikal Lumbangaol ◽

Risanti F. R. Situmorang ◽

Saronom Silaban ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Extraction Process ◽

Total Phenolic ◽

Betel Leaf ◽

Dpph Method

The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical screening of the ethanolic extract of red betel leaf contain secondary metabolites, including flavonoid, phenolic, tannin, alkaloids, steroids, and triterpenoids. The total phenolic content of the red betel leaf ethanol extract was 0.949±0.003 mg GAE/g d.w. and has antioxidant activity (IC50) 84,656 including strong category as an antioxidant. Keywords: Piper crocatum Ruiz & Pav., Antioxidant, Ethanol extract, Folin-Ciocalteu and DPPH

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Antioxidant Activity of n-Hexane, Ethyl Acetate and Ethanol Extract from Lakoocha Leaves (Artocarpus lacucha Buch.-Ham) using DPPH Method

Indonesian Journal of Pharmaceutical and Clinical Research ◽

10.32734/idjpcr.v1i2.433 ◽

2018 ◽

Vol 1 (2) ◽

pp. 41-47

Author(s):

Poppy Anjelisa Zaitun Hasibuan ◽

Mardiana

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Radical Scavenging ◽

Ethanol Extract ◽

Positive Control ◽

Hexane Extract ◽

Phytochemical Screening ◽

Ic50 Value ◽

Dpph Method

This study aimed to investigate phytochemical screening and antioxidant activity of n–hexane, ethyl acetate and ethanol extract from lakoocha leaves. The powdered simplicia was macerated with n–hexane, ethyl acetate and ethanol 96% successively, filtered, then concentrated using rotary evaporator to obtain n–hexane extract, ethyl acetate extract and ethanol extract. Phytochemical screening and antioxidant activity was performed against these extracts. Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method using ultraviolet-visible spectrophotometer at wavelength of 516 nm after incubated for 60 minutes in dark place. Quercetin was used as positive control. The result of phytochemical screening showed n-hexane extract contains steroid, ethyl acetate extract contain steroid, tannin, glycoside, flavonoid and saponin, whereas ethanol extract contain tannin, glycoside, flavonoid and saponin. The IC50 value of n–hexane, ethyl acetate and ethanol extract was 1062.03±1.42 ppm, 323.18±0.02 ppm and 99.23±0.07 ppm respectively, whereas for quercetin was 2.32±0.01 ppm. This study showed that ethanol extract had antioxidant activity with strong category whereas n-hexane extract and ethyl acetate extract had inactive antioxidant activity with very weak categories. Keyword: Antioxidant Activity, DPPH, Lakoocha leaf

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Pengaruh Metode Pengeringan Terhadap Aktivitas Antioksidan Ekstrak Etanol

Cendekia Journal of Pharmacy ◽

10.31596/cjp.v3i1.43 ◽

2019 ◽

Vol 3 (1) ◽

pp. 44-52

Author(s):

Endra Pujiastuti ◽

Rahma Sani Saputri

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Ethanol Extract ◽

Drying Methods ◽

Drying Method ◽

Oven Method ◽

Ic50 Value ◽

Dpph Method ◽

Qualitative Test ◽

High Antioxidant Activity

Drying is the most important step to keep the compound stability in simplicia and is very influential to produce good quality igredients in the antioxidant activity contained in the simplicia. Antioxidants are oxidation-free inhibitors of free radical reactions. Parijoto is one of the plants that have antioxidant compounds.This study aims to determine the effect of drying, concentration of antioxidant activity of parijoto (Medinilla speciosa Blume) fruit. The drying method used is direct sunlight drying, indirect sunlight and oven. The antioxidant activity of ethanol extract on drying method was determined by 2,2 diphenyl-1-picrylhidrazyl (DPPH) method which is free radical. The qualitative test DPPH showed that the higher levels of ethanol extract from the three drying methods further diminished the color of DPPH solution in ethanol.The result showed thet the drying method gave different effect to the antioxidant activity of parijoto fruit which is significant(p0,05). Drying methods wich have high antioxidant activity are given by drying using oven method with an IC50 value 33,75µg/ml.

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