Escherichia Coli Isolated From Raw Milk at North Sinai Governorate

2017 ◽  
Vol 22 (2) ◽  
pp. 121-131
Author(s):  
Ahmed Khafagy ◽  
Hamza Eid ◽  
Fatma Youssif ◽  
Nour El Houda Eid
Keyword(s):  
Escherichia Coli ◽  
Raw Milk ◽  
North Sinai

scholarly journals Genome Sequence and Annotation of a Human Infection Isolate of Escherichia coli O26:H11 Involved in a Raw Milk Cheese Outbreak

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Wessam Galia ◽  
Patricia Mariani-Kurkdjian ◽  
Estelle Loukiadis ◽  
Stéphanie Blanquet-Diot ◽  
Françoise Leriche ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genome Sequence ◽  
Raw Milk ◽  
Human Infection ◽  
Raw Milk Cheese

scholarly journals Prevalence and molecular characterisation of shiga-toxin-producing Escherichia coli in raw milk cheeses from Lazio region, Italy

2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Selene Marozzi ◽  
Paola De Santis ◽  
Sarah Lovari ◽  
Roberto Condoleo ◽  
Stefano Bilei ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Raw Milk ◽  
Foodborne Diseases ◽  
Control Plan ◽  
Lazio Region ◽  
Standard Methods ◽  
E Coli ◽  
Regional Control ◽  
Unpasteurized Milk

In recent years, the incidence of foodborne diseases caused by shiga toxin-producing <em>Escherichia coli</em> (STEC) has increased globally. For this reason, within the specific regional control plan for the detection of STEC in food products in Italy, the presence of STEC in unpasteurized milk cheeses was investigated. In total 203 samples obtained from March 2011 to December 2013 were analysed, with two standard methods (ISO 16654:2001 and ISO 13136:2012). Two strains of <em>E. coli</em> O157 were isolated (2/161, 1.2%) but did not carry any virulence-associated genes and 22 <em>stx</em>-positive samples (22/146, 15.1%) were detected in enrichment cultures, mostly from ovine cheeses. Only two strains isolated from different ovine cheeses carried <em>stx</em> gene and none of these was <em>eae</em>-positive. This study confirms the presence of <em>stx</em>-positive <em>E. coli</em> and suggests that this type of food cannot be excluded as a potential vehicle of STEC.

Characteristics of Shiga Toxin–Producing Escherichia coli Isolated from Swiss Raw Milk Cheese within a 3-Year Monitoring Program

2010 ◽  
Vol 73 (1) ◽  
pp. 88-91 ◽  
Author(s):  
C. ZWEIFEL ◽  
N. GIEZENDANNER ◽  
S. CORTI ◽  
G. KRAUSE ◽  
L. BEUTIN ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Monitoring Program ◽  
Raw Milk ◽  
Health Impact ◽  
Toxin Gene ◽  
Genes Encoding ◽  
Raw Milk Cheese ◽  
Human Infections ◽  
Hard Cheese

Food is an important vehicle for transmission of Shiga toxin–producing Escherichia coli (STEC). To assess the potential public health impact of STEC in Swiss raw milk cheese produced from cow's, goat's, and ewe's milk, 1,422 samples from semihard or hard cheese and 80 samples from soft cheese were examined for STEC, and isolated strains were further characterized. By PCR, STEC was detected after enrichment in 5.7% of the 1,502 raw milk cheese samples collected at the producer level. STEC-positive samples comprised 76 semihard, 8 soft, and 1 hard cheese. By colony hybridization, 29 STEC strains were isolated from 24 semihard and 5 soft cheeses. Thirteen of the 24 strains typeable with O antisera belonged to the serogroups O2, O22, and O91. More than half (58.6%) of the 29 strains belonged to O:H serotypes previously isolated from humans, and STEC O22:H8, O91:H10, O91:H21, and O174:H21 have also been identified as agents of hemolytic uremic syndrome. Typing of Shiga toxin genes showed that stx1 was only found in 2 strains, whereas 27 strains carried genes encoding for the Stx2 group, mainly stx2 and stx2vh-a/b. Production of Stx2 and Stx2vh-a/b subtypes might be an indicator for a severe outcome in patients. Nine strains harbored hlyA (enterohemorrhagic E. coli hemolysin), whereas none tested positive for eae (intimin). Consequently, semihard and hard raw milk cheese may be a potential source of STEC, and a notable proportion of the isolated non-O157 STEC strains belonged to serotypes or harbored Shiga toxin gene variants associated with human infections.

scholarly journals Occurrence of toxigenic Escherichia coli in raw milk cheese in Brazil

2007 ◽  
Vol 59 (2) ◽  
pp. 508-512 ◽  
Author(s):  
B.R. Paneto ◽  
R.P. Schocken-Iturrino ◽  
C. Macedo ◽  
E. Santo ◽  
J.M. Marin
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Antimicrobial Agents ◽  
Raw Milk ◽  
Diffusion Method ◽  
Chain Reaction ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Polymerase Chain ◽  
Raw Milk Cheese

The occurrence of toxigenic Escherichia coli in raw milk cheese was surveyed in Middle Western Brazil. Fifty samples of cheese from different supermarkets were analyzed for E.coli. The isolates were serotyped and screened for the presence of verotoxigenic E. coli (VTEC) and enterotoxigenic E. coli (ETEC) by Polymerase Chain Reaction (PCR). The susceptibility to thirteen antimicrobial agents was evaluated by the disk diffusion method. E.coli were recovered from 48 (96.0%) of the samples. The serogroups identified were O125 (6.0%), O111 (4.0%), O55 (2.0%) and O119 (2.0%). Three (6.0%) and 1(2.0%) of the E.coli isolates were VTEC and ETEC, respectively. Most frequent resistance was observed to the following antimicrobials: cephalothin (60.0%), nalidixic acid (40.0%), doxycyclin (33.0%), tetracycline (31.0%) and ampicillin (29.0%).

Incidence of Escherichia coli O157 in raw milk and white pickled cheese manufactured from raw milk in Turkey

Food Control ◽  
2004 ◽  
Vol 15 (6) ◽  
pp. 453-456 ◽  
Author(s):  
Ö Öksüz ◽  
M Arici ◽  
S Kurultay ◽  
T Gümüs
Keyword(s):  
Escherichia Coli ◽  
Raw Milk ◽  
Escherichia Coli O157

scholarly journals The influence of the climatology of the semi-arid from Alagoas-Brazil on the raw milk microbiota

2017 ◽  
Vol 3 (1) ◽  
pp. 27
Author(s):  
Ticiano Gomes do Nascimento ◽  
Euridice Farias Falcão ◽  
Maria Cristina Delgado da Silva ◽  
Josicleide Nascimento Oliveira Silvino ◽  
Pierre Barnabé Escodro ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Thermal Comfort ◽  
Vegetation Index ◽  
Raw Milk ◽  
Climatic Conditions ◽  
The State ◽  
Maximum Temperature ◽  
Climatic Data ◽  
Microbiological Analysis ◽  
Semi Arid

This study aimed to evaluate the influence of the climatology of the semi-arid from Alagoas-Brazil on the raw milk microbiota in semi-arid area of the 07 micro-regions of the State of Alagoas of Alagoas, Brazil. The climatic data were extracted from National Institute of Meteorology from the Brazilian government. The raw milk was collected after the dairy cow milking process in 12 small rural associations of the semi-arid from the State of Alagoas, during the 4 seasons and the raw milk was carried out procedures of sampling, transportation and microbiological analysis. A total of 58 samples were counted coliforms at 45°C, <em>Escherichia coli</em> and coagulase-positive <em>Staphylococcus</em>. Only 02 rural associations presented low levels of microbiological contamination, which were located in areas of climatic conditions and parameters of thermal comfort index and vegetation index favorable, but 10 rural associations presented high counting of coliforms at 45°C, <em>Escherichia coli</em>. The climatologic parameters (maximum temperature, atmospheric pressure), bovine comfort thermal index and vegetation index have showed to influence the growth of the coliforms at 45°C and <em>Escherichia coli</em> with high incidence during the summer weather. The precipitation parameter, bovine thermal comfort and vegetation index have displayed to influence coagulase-positive<em> Staphylococcus</em> counting especially during the period between the summer end and the autumn beginning seasons. New Actions, and Rural Education and Health Programs should be implemented as politics of Food Safety. New strategies and programs for dissemination more effective on the risks of transmission of pathogens and Foodborne Diseases are necessary as the part of emergence politics of the health and education areas. Regulatory Actions should be encouraged within the processes that improve the quality control of raw milk as well its bioproducts, with professional assistance relevant in agriculture area.

Flourogenic Assays for Rapid Detection of Escherichia coli in Tap Water and Raw Milk Samples

2003 ◽  
Vol 6 (8) ◽  
pp. 770-773 ◽  
Author(s):  
A.K. Azad ◽  
Fauzia Hafiz . ◽  
S.M. Badiar Rahman .
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Tap Water ◽  
Raw Milk ◽  
Milk Samples

scholarly journals Detection of virulence genes and antimicrobial resistance profiles of Escherichia coli isolates from raw milk and artisanal cheese in Southern Brazil

2019 ◽  
Vol 40 (1) ◽  
pp. 163 ◽  
Author(s):  
Leandro Parussolo ◽  
Ricardo Antônio Pilegi Sfaciotte ◽  
Karine Andrezza Dalmina ◽  
Fernanda Danielle Melo ◽  
Ubirajara Maciel Costa ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Virulence Genes ◽  
Antimicrobial Agents ◽  
Raw Milk ◽  
Diffusion Method ◽  
Public Health Issue ◽  
Southern Brazil ◽  
E Coli

The serrano artisanal cheese is a typical product from South region of Brazil, which is produced by skilled cheesemakers using raw milk. The contamination of this food by Escherichia coli has a great impact on public health, since it could threat the consumers’ health. The study evaluated the presence of virulence genes, antimicrobial susceptibility profiles and bofilm-production ability of Escherichia coli isolates obtained from raw milk and artisanal cheese produced in Southern Brazil. A total of 117 isolates of E. coli were characterized by multiplex PCR to detect the following virulence genes: eae for enteropatogenic E. coli (EPEC), lt and st for enterotoxigenic E. coli (ETEC), stx for shiga toxin-producing E. coli (STEC), stx and eae for enterohemorrhagic E. coli (EHEC), ipaH for enteroinvasive E. coli (EIEC) and aggR for enteroaggregative E. coli (EAEC). In addition, antimicrobial susceptibility profile to 22 antimicrobial agents was also performed by disk diffusion method, and we searched for extended-spectrum beta-lactamases (ESBL) and/or carbapenemase- producing isolates. Isolates that were positive for ESBL and carbapenemase were further investigated for the presence of the genes: blaTEM, blaSHV, blaOXA, blaCTX-M, for ESBL and blaOXA-48 for carbapenemase. Further, isolates had their ability to form biofilms investigated by the red Congo agar method. Virulence genes of E. coli were identified in 21.37% of the tested isolates, which were classified as EPEC (the most prevalent pathotype) and ETEC or EAEC. Ten (8.55%) of the total studied E. coli isolates revealed a multidrug-resistant profile, since they were resistant to three or more antimicrobial classes; whereas four isolates (3.42%) were classified as ESBL-producers and showed the presence of blaTEM gene. None of the isolates exhibited carbapenemase activity nor did they carry carbapenemase genes. From the total of E. coli isolates, 79 (67.52%) were considered potential biofilm producers. These results address a serious public health issue, since artisanal cheeses pose a risk to consumers’ health, since may be sources of dissemination of diarrheogenic E. coli, that can cause from subclinical to severe and fatal infections in children and adults, and also emphasize the need to improve adaptations/adjustments in the manufacturing processes of these products.

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