GENETIC PROFILING AND DIVERSITY OF SOME PROMISING EFFICIENT RHIZOBIAL ISOLATES ON FABA BEAN PLANTS

Pathogenesis-related (PR) proteins are known to play relevant roles in plant defense against biotic and abiotic stresses. In the present study, we characterize the response of transgenic faba bean (Vicia faba L.) plants encoding a PR10a gene from potato (Solanum tuberosum L.) to salinity and drought. The transgene was under the mannopine synthetase (pMAS) promoter. PR10a-overexpressing faba bean plants showed better growth than the wild-type plants after 14 days of drought stress and 30 days of salt stress under hydroponic growth conditions. After removing the stress, the PR10a-plants returned to a normal state, while the wild-type plants could not be restored. Most importantly, there was no phenotypic difference between transgenic and non-transgenic faba bean plants under well-watered conditions. Evaluation of physiological parameters during salt stress showed lower Na+-content in the leaves of the transgenic plants, which would reduce the toxic effect. In addition, PR10a-plants were able to maintain vegetative growth and experienced fewer photosystem changes under both stresses and a lower level of osmotic stress injury under salt stress compared to wild-type plants. Taken together, our findings suggest that the PR10a gene from potato plays an important role in abiotic stress tolerance, probably by activation of stress-related physiological processes.

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Foliar-Applied Potassium Silicate Coupled with Plant Growth-Promoting Rhizobacteria Improves Growth, Physiology, Nutrient Uptake and Productivity of Faba Bean (Vicia faba L.) Irrigated with Saline Water in Salt-Affected Soil

Plants ◽

10.3390/plants10050894 ◽

2021 ◽

Vol 10 (5) ◽

pp. 894

Author(s):

Emad M. Hafez ◽

Hany S. Osman ◽

Usama A. Abd El-Razek ◽

Mohssen Elbagory ◽

Alaa El-Dein Omara ◽

...

Keyword(s):

Plant Growth ◽

Fresh Water ◽

Faba Bean ◽

Saline Water ◽

Plant Growth Promoting Rhizobacteria ◽

Control Treatment ◽

Combined Application ◽

Bean Plants ◽

Plant Growth Promoting ◽

Growth Promoting

The continuity of traditional planting systems in the last few decades has encountered its most significant challenge in the harsh changes in the global climate, leading to frustration in the plant growth and productivity, especially in the arid and semi-arid regions cultivated with moderate or sensitive crops to abiotic stresses. Faba bean, like most legume crops, is considered a moderately sensitive crop to saline soil and/or saline water. In this connection, a field experiment was conducted during the successive winter seasons 2018/2019 and 2019/2020 in a salt-affected soil to explore the combined effects of plant growth-promoting rhizobacteria (PGPR) and potassium (K) silicate on maintaining the soil quality, performance, and productivity of faba bean plants irrigated with either fresh water or saline water. Our findings indicated that the coupled use of PGPR and K silicate under the saline water irrigation treatment had the capability to reduce the levels of exchangeable sodium percentage (ESP) in the soil and to promote the activity of some soil enzymes (urease and dehydrogenase), which recorded nearly non-significant differences compared with fresh water (control) treatment, leading to reinstating the soil quality. Consequently, under salinity stress, the combined application motivated the faba bean vegetative growth, e.g., root length and nodulation, which reinstated the K+/Na+ ions homeostasis, leading to the lessening or equalizing of the activity level of enzymatic antioxidants (CAT, POD, and SOD) compared with the controls of both saline water and fresh water treatments, respectively. Although the irrigation with saline water significantly increased the osmolytes concentration (free amino acids and proline) in faba bean plants compared with fresh water treatment, application of PGPR or K-silicate notably reduced the osmolyte levels below the control treatment, either under stress or non-stress conditions. On the contrary, the concentrations of soluble assimilates (total soluble proteins and total soluble sugars) recorded pronounced increases under tested treatments, which enriched the plant growth, the nutrients (N, P, and K) uptake and translocation to the sink organs, which lastly improved the yield attributes (number of pods plant−1, number of seeds pod−1, 100-seed weight). It was concluded that the combined application of PGPR and K-silicate is considered a profitable strategy that is able to alleviate the harmful impact of salt stress alongside increasing plant growth and productivity.

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First Detection of Faba bean necrotic yellow virus in Spain

Plant Disease ◽

10.1094/pdis.2000.84.6.707b ◽

2000 ◽

Vol 84 (6) ◽

pp. 707-707 ◽

Cited By ~ 7

Author(s):

M. Babin ◽

V. Ortíz ◽

S. Castro ◽

J. Romero

Keyword(s):

Faba Bean ◽

Acyrthosiphon Pisum ◽

Virus Disease ◽

Epidemiological Data ◽

Aphid Transmission ◽

Enzyme Linked Immunosorbent Assay ◽

Yellow Virus ◽

Potential Threat ◽

Bean Plants ◽

Faba Beans

Faba bean necrotic yellow virus (FBNYV) was not detected during 1994 to 1996 field surveys of faba beans (Vicia faba L.) in Spain (1). In 1997, however, one sample with symptoms of necrosis, collected in Baleares, was tested using ELISA (enzyme-linked immunosorbent assay) and was positive for both Tomato spotted wilt virus (TSWV) and FBNYV. FBNYV is a single-strand DNA virus that is transmitted by aphids and is the main virus disease of broad bean in North Africa and West Asia (2). During 1997 to 1999, faba bean plants with symptoms of necrosis, yellowing, small leaves, and stunting were collected from several fields in the Murcia Region (Spain) and were analyzed using ELISA. To detect FBNYV, we used monoclonal 2E9 supplied by H. J. Vetten (Institute of Plant Virology, Microbiology and Biosafety, BBA, Braunschweig, Germany). Of 700 samples analyzed, 34 were positive for FBNYV. Of the 34 positive samples, 12 tested positive, using commercial antiserum from Loewe, Inc. (Munich) for mixed infections with TSWV. FBNYV was transmitted to healthy faba bean plants by aphids (Acyrthosiphon pisum) in greenhouse experiments and was confirmed by ELISA. Preliminary epidemiological data showed a gradual increase in the number of plants infected with time in the same field. Aphid transmission of FBNYV to faba beans has established the disease in Spain and is a potential threat to other leguminous crops. This is the first report of a nanovirus in Europe. References: (1) J. Fresno et al. Plant Dis. 81:112, 1997. (2) L. Katul et al. Ann. Appl. Biol. 123:629, 1993.

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Isolation and Assessment of the Symbiotic Efficiency of Faba bean (Vicia faba L.) Nodulating Rhizobial Isolates from Eastern Hararghe Highlands of Ethiopia

Current Agriculture Research Journal ◽

10.12944/carj.9.2.04 ◽

2021 ◽

Vol 9 (2) ◽

pp. 91-105

Author(s):

Mulugeta Mekonnen ◽

Ameha Kebede

Keyword(s):

Vicia Faba ◽

Faba Bean ◽

Dry Weight ◽

Symbiotic Efficiency ◽

Shoot Dry Weight ◽

Nacl Concentration ◽

Vicia Faba L ◽

Positive Correlations ◽

Ph Range ◽

Rhizobial Isolates

This particular work was devoted to isolate and assess the symbiotic efficiency of faba bean (Vicia faba L.)-nodulating rhizobia isolate at few faba bean growing areas of the eastern Hararghe highlands of Ethiopia. Overall 50 rhizobia isolates were obtained from soil samples of three Woredas (districts) of the eastern Hararghe highlands using the host trap method. Out of these 50 isolates, 40 were presumptively identified as rhizobia. Among these 40 rhizobia isolates, only 31 were successful to nodulate faba bean, and authenticated as true faba bean nodulating rhizobia. Concerning the symbiotic efficiency, about 52%, 35%, and 13% of the rhizobial isolates were found to be highly effective, effective, and lowly-effective, respectively. The correlation data on the sand experiment displayed that nodule dry weight was associated positively and significantly (r = 0.494, p<0.05) with shoot dry weight while shoot dry weight was associated positively and significantly (r=0.41, p<0.05) with plant total nitrogen. Positive correlations were also observed concerning shoot dry weight and dry weight of nodules (r = 0.7, p<0.05) on unsterilized soil. Among the observed rhizobium isolates, EHHFR (4A, 6A) showed the highest symbiotic efficiency above 110%, tolerated NaCl concentration ranging from 2% to 6% and 2% to 8%, respectively, and a pH range of 4.5 to 8 and 5 to 8, respectively. Thus, based on their symbiotic efficiency at the greenhouse level and relative tolerance to extreme conditions these faba bean nodulating rhizobia isolates were recommended to be used as nominees for the future development of faba bean rhizobial inoculants after being tested on field conditions.

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EFFECT OF SALICYLIC ACID AND ABSCISIC ACID ON MORPHO-PHYSIOLOGICAL AND ANATOMICAL CHARACTERS OF FABA BEAN PLANTS (Vicia faba L.) UNDER DROUGHT STRESS

Journal of Plant Production ◽

10.21608/jpp.2015.52096 ◽

2015 ◽

Vol 6 (11) ◽

pp. 1771-1788 ◽

Cited By ~ 9

Author(s):

Kh. Abdelaal

Keyword(s):

Salicylic Acid ◽

Abscisic Acid ◽

Drought Stress ◽

Vicia Faba ◽

Faba Bean ◽

Bean Plants ◽

Vicia Faba L ◽

Anatomical Characters

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Morphological, Biochemical and Molecular Characterization of Rhizobia of Faba Bean Plants Grown in North Nile Delta Egypt

Pakistan Journal of Biological Sciences ◽

10.3923/pjbs.2021.672.679 ◽

2021 ◽

Vol 24 (6) ◽

pp. 672-679

Author(s):

Doha M. Fathy ◽

Ahmed S. Eldomiaty ◽

Hassan I. Abd El- ◽

El-Sayed I. Mahgoub ◽

Abdallah A. Hassani

Keyword(s):

Molecular Characterization ◽

Faba Bean ◽

Nile Delta ◽

Bean Plants

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Effect of cadmium on growth and oxidative metabolism of faba bean plants

Acta Agronomica Hungarica ◽

10.1556/aagr.52.2004.3.8 ◽

2004 ◽

Vol 52 (3) ◽

pp. 269-276 ◽

Cited By ~ 7

Author(s):

H. R. Moussa

Keyword(s):

Oxidative Stress ◽

Faba Bean ◽

Photosynthetic Efficiency ◽

Nutrient Status ◽

Significant Inhibition ◽

Free Proline ◽

Rubisco Activity ◽

Bisphosphate Carboxylase ◽

Inhibition Of Growth ◽

Bean Plants

The effect of CdCl2(0-50 µM) on the growth, physiological parametersand leaf antioxidative enzymes of faba bean plants was studied in order toinvestigate the possible involvement of this metal in the generationof oxidative stress. In the roots and leaves of faba bean plants Cd produceda significant inhibition of growth, as well as a reduction inthe transpiration rate, photosynthetic efficiency (14CO2-fixation), ribulose-1,5-bisphosphate-carboxylase/oxygenase (Rubisco) activity and leaf pigment content, and an alteration in the nutrient status in booth roots and leaves. an increased level of free proline was also detected. The results suggest thatthe treatment of faba bean plants with CdCl2 induced a concentration-dependentoxidative stress situation in the leaves, characterized by an accumulationof H2O2, as a result of theinhibition of the antioxidant enzymes glutathione reductase (GR) and catalase (CAT). These results point to the possible inductionof leaf senescence by cadmium.

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Molecular Identification of Rhizobium Isolates Nodulating Faba Bean Plants in Egyptian Soils

Journal of Bioprocessing & Biotechniques ◽

10.4172/2155-9821.1000194 ◽

2015 ◽

Vol 05 (01) ◽

Author(s):

Abdel Fattah Mohamed Elsobky

Keyword(s):

Molecular Identification ◽

Faba Bean ◽

Bean Plants

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Effects of continuous combined nitrogen supply on symbiotic dinitrogen fixation of faba bean and pea inoculated with different rhizobial isolates

Canadian Journal of Botany ◽

10.1139/b87-345 ◽

1987 ◽

Vol 65 (12) ◽

pp. 2542-2548 ◽

Cited By ~ 18

Author(s):

François-P. Chalifour ◽

Louise M. Nelson

Keyword(s):

Faba Bean ◽

Acetylene Reduction ◽

Rhizobium Leguminosarum ◽

Combined Nitrogen ◽

Pisum Sativum L ◽

Legume Symbiosis ◽

Total Plant ◽

Continuous Presence ◽

Symbiotic Partner ◽

Rhizobial Isolates

Combined nitrogen (N) has adverse effects on virtually all stages of the Rhizobium–legume symbiosis. Tolerance to combined N varies among legume hosts and rhizobial isolates, but the contribution of each symbiotic partner is not well established. The effects of combined N were studied in faba bean (Vicia faba L.) and pea (Pisum sativum L.), using the same Rhizobium leguminosarum isolates for both hosts. In one experiment, faba bean and pea were inoculated individually with four rhizobial isolates and grown for 28 days in the continuous presence of 0, 2.5, 5.0, or7.5 mol m−3 NH4NO3. For each isolate, faba bean was consistently more tolerant to combined N than pea as shown by significantly smaller rates of decrease in N2-fixing activity (acetylene reduction) in faba bean than in pea. The results were substantiated by those of a similar experiment in which increasing levels of 15N-labeled [Formula: see text] (5, 10, or 15 mol m−3) were supplied continuously to faba bean and pea inoculated individually with two rhizobial isolates. Comparisons of the different symbioses based on the proportion of total plant N derived from N2 fixation confirmed the conclusions reached using acetylene reduction activities.

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