scholarly journals Cryptosporidium and Giardia in recreational water in Belgium

2015 ◽  
Vol 13 (3) ◽  
pp. 870-878 ◽  
Author(s):  
Amimul Ehsan ◽  
Stijn Casaert ◽  
Bruno Levecke ◽  
Liesbet Van Rooy ◽  
Joachim Pelicaen ◽  
...  

The objective of this study was to investigate the presence of Cryptosporidium and Giardia in different recreational water bodies in Belgium and to estimate the infection risk associated with swimming and other recreational activities. Cryptosporidium oocysts and/or Giardia cysts were detected in three out of 37 swimming pools, seven out of 10 recreational lakes, two out of seven splash parks and four out of 16 water fountains. In the swimming pools no infection risk for Cryptosporidium could be calculated, since oocysts were only detected in filter backwash water. The risk of Giardia infection in the swimming pools varied from 1.13 × 10−6 to 2.49 × 10−6 per swim per person. In recreational lakes, the infection risk varied from 2.79 × 10−5 to 5.74 × 10−5 per swim per person for Cryptosporidium and from 7.04 × 10−5 to 1.46 × 10−4 for Giardia. For other outdoor water recreation activities the estimated infection risk was 5.71 × 10−6 for Cryptosporidium and 1.47 × 10−5 for Giardia. However, most positive samples in the recreational lakes belonged to species/genotypes that are either animal-specific or predominantly found in animals. No Cryptosporidium was found in splash parks and water fountains, but the presence of Giardia cysts suggests a risk for human infection. The infection risk of Giardia infection during a 3.5-minute visit to a splash park for children equalled 1.68 × 10−4.

2020 ◽  
Vol 67 (7) ◽  
pp. 785-795
Author(s):  
Carolina Ortiz Pineda ◽  
Diego Averaldo Guiguet Leal ◽  
Vagner Ricardo da Silva Fiuza ◽  
Juliana Jose ◽  
Guilherme Borelli ◽  
...  

2004 ◽  
Vol 2 (3) ◽  
pp. 191-200 ◽  
Author(s):  
F. M. Schets ◽  
G. B. Engels ◽  
E. G. Evers

The occurrence of Cryptosporidium and Giardia in indoor swimming pools in the Netherlands was studied at five locations. The backwash water from seven pool filters was analysed for the presence of Cryptosporidium oocysts and Giardia cysts for a period of 1 year. Of the 153 samples of filter backwash water analysed, 18 (11.8%) were found positive for either Cryptosporidium (4.6%), Giardia (5.9%) or both (1.3%). Oocysts and cysts were also detected in the water of one toddler pool and one learner pool. Although most of the (oo)cysts in the filter backwash water were dead, viable and potentially infectious oocysts were detected in the learner pool. On the basis of numbers of potentially infectious (oo)cysts detected in the learner pool, and assuming one visit to an infected pool per year, risk assessment indicated an estimated risk of infection with Cryptosporidium that exceeded the generally accepted risk of one infection per 10,000 persons per year. Guidelines for pool operators on how to manage faecal accidents and public information on the importance of hygiene in swimming pool complexes are recommended tools in controlling the risk of infection.


Author(s):  
T. Sampson ◽  
L. K. Giami ◽  
J. A. Okedike

Recreational water bodies are water bodies used for recreational activities such as swimming, surfing, water skiing, water diving and sailing. They include rivers, lakes, beaches, spas and swimming pools. This work was therefore aimed at determining the bacteriological profile of recreational water bodies in Orashi region of Rivers State, Nigeria. Surface water samples were collected from two different sites (Orashi River, Mbiama and Sombreiro River, Ahoada) using standard microbiological methods. Upstream, midstream and downstream samples were collected for a period of three months at monthly interval. Standard plate counts were used for total heterotrophic and coliform bacterial counts using standard microbiological media. The total heterotrophic bacterial count ranged from 4.1X104 to 9.5X104 for Orashi River and 3.0X103 to 4.0X103 for Sombreiro River. A significant statistical difference (p < 0.05) however, existed between total heterotrophic bacterial counts of the samples collected from Orashi River, while no statistical difference (p > 0.05) was observed in the total heterotrophic bacterial counts of samples from Sombreiro River. In the comparative analysis of the samples from the two water bodies, no statistical difference (p > 0.05) was recorded in the total coliform count in Orashi and Sombreiro Rivers. The phenotypic characterization identified the isolates to include Staphylococcus spp., Klebsiella spp., Pseudomonas spp., Bacillus spp., Enterococcus spp. and Micrococcus spp., with Klebsiella pneumoniae as the most occurring (26.1%). Klebsiella pneumoniae and Staphylococcus aureus are known for their pathogenic potentials, hence their presence in these recreational sites are of public health importance. Provision of standard recreational facilities in localities will however reduce the dependency on river sites for recreational activities, and as well prevent recreational associated illnesses.


2011 ◽  
Vol 9 (4) ◽  
pp. 653-658 ◽  
Author(s):  
James E. Amburgey ◽  
J. Brian Anderson

Cryptosporidium is a chlorine-resistant protozoan parasite responsible for the majority of waterborne disease outbreaks in recreational water venues in the USA. Swim diapers are commonly used by diaper-aged children participating in aquatic activities. This research was intended to evaluate disposable swim diapers for retaining 5-μm diameter polystyrene microspheres, which were used as non-infectious surrogates for Cryptosporidium oocysts. A hot tub recirculating water without a filter was used for this research. The microsphere concentration in the water was monitored at regular intervals following introduction of microspheres inside of a swim diaper while a human subject undertook normal swim/play activities. Microsphere concentrations in the bulk water showed that the majority (50–97%) of Cryptosporidium-sized particles were released from the swim diaper within 1 to 5 min regardless of the swim diaper type or configuration. After only 10 min of play, 77–100% of the microspheres had been released from all swim diapers tested. This research suggests that the swim diapers commonly used by diaper-aged children in swimming pools and other aquatic activities are of limited value in retaining Cryptosporidium-sized particles. Improved swim diaper solutions are necessary to efficiently retain pathogens and effectively safeguard public health in recreational water venues.


2014 ◽  
Vol 96 ◽  
pp. 68-69 ◽  
Author(s):  
Lucy J. Robertson ◽  
Stijn Casaert ◽  
Yazel Valdez-Nava ◽  
Md. Amimul Ehsan ◽  
Edwin Claerebout

2000 ◽  
Vol 41 (7) ◽  
pp. 31-37 ◽  
Author(s):  
E. Carraro ◽  
E. Fea ◽  
S. Salva ◽  
G. Gilli

The aim of this study was to assess the impact of a municipal wastewater treatment plant (MWTP) on the occurrence of Cryptosporidium oocysts and Giardia cysts in the receiving water. All MWTP effluent samples were Giardia and Cryptosporidium contaminated, although low mean values were found for both parasites (0.21±0.06 oocysts/L; 1.39±0.51 cysts/L). Otherwise, in the raw sewage a greater concentration was detected (4.5±0.3 oocysts/L; 53.6±6.8 cysts/L). The major occurrence of Giardia over Cryptosporidium, both in the influent and in the effluent of the MWTP, is probably related to the human sewage contribution to the wastewater. Data on protozoa contamination of the receiving water body demonstrated similar concentrations in the samples collected before (0.21±0.07 oocysts/L; 1.31±0.38 cysts/L) and after (0.17±0.09 oocysts/L and 1.01±1.05 cysts/L) the plant effluent discharge. The results of this study suggest that the MWTP has no impact related to Giardia and Cryptosporidium river water contamination, and underline the need for investigation into the effectiveness of these protozoa removal by less technologically advanced MWTPs which are the most widespread and could probably show a lower ability to reduce protozoa.


2007 ◽  
Vol 55 (8-9) ◽  
pp. 377-385 ◽  
Author(s):  
S. Skraber ◽  
K. Helmi ◽  
R. Willame ◽  
M. Ferréol ◽  
C. Gantzer ◽  
...  

Biofilms within wastewater treatment plants can capture enteric microorganisms initially present in the water phase immobilising them either definitively or temporarily. Consequently, fates of microorganisms may totally change depending on whether they interact or not with biofilms. In this study, we assessed the stability of wastewater biofilms comparing the evolution of the concentrations of bacteria (heterotrophic plate count [HPC], thermotolerant coliforms [TC]) and viral (somatic coliphages [SC] and F-specific phages [F+]) indicators in the biofilms and in the corresponding wastewaters at 4 and 20 °C. Additionally, we assessed the monthly occurrence of these bacterial and viral indicators as well as of pathogenic protozoa (Cryptosporidium oocysts and Giardia cysts) in three native wastewater biofilms for four months. Our results show that viral indicators (SC and F+) persist longer in biofilms than in the corresponding wastewaters at 4 °C as well as at 20 °C. In contrast, persistence of bacterial indicators (TC and HPC) depends on both the temperature and the matrix. Differences between viral and bacterial persistence are discussed. Monthly analysis of native wastewater biofilms shows that bacterial and viral indicators, as well as Cryptosporidium oocysts and Giardia cysts, attach to wastewater biofilms to a concentration that remains stable in time, probably as a result of a dynamic equilibrium between attachment and detachment processes.


2021 ◽  
Author(s):  
Max T Eyre ◽  
Fábio N Souza ◽  
Ticiana S. A. Carvalho-Pereira ◽  
Nivison Nery ◽  
Daiana de Oliveira ◽  
...  

Background: Zoonotic spillover from animal reservoirs is responsible for a significant global public health burden, but the processes that promote spillover events are poorly understood in complex urban settings. Endemic transmission of Leptospira, the agent of leptospirosis, in marginalised urban communities occurs through human exposure to an environment contaminated by bacteria shed in the urine of the rat reservoir. However, it is unclear to what extent transmission is driven by variation in the distribution of rats or by the dispersal of bacteria in rainwater runoff and overflow from open sewer systems. <br /><br />Methods: We conducted an eco-epidemiological study in a high-risk community in Salvador, Brazil, by prospectively following a cohort of 1,401 residents to ascertain serological evidence for leptospiral infections. A concurrent rat ecology study was used to collect information on the fine-scale spatial distribution of ‘rattiness’, our proxy for rat abundance and exposure of interest. We developed and applied a novel geostatistical framework for joint spatial modelling of multiple indices of disease reservoir abundance and human infection risk. <br /><br />Results: The estimated infection rate was 51.4 (95\%CI 40.4, 64.2) infections per 1,000 follow-up events. Infection risk increased with age until 30 years of age and was associated with male gender. Rattiness was positively associated with infection risk for residents across the entire study area, but this effect was stronger in higher elevation areas (OR 3.27 95\%CI 1.68, 19.07) than in lower elevation areas (OR 1.14 95\%CI 1.05, 1.53). <br /><br />Conclusions: These findings suggest that, while frequent flooding events may disperse bacteria in regions of low elevation, environmental risk in higher elevation areas is more localised and directly driven by the distribution of local rat populations. The modelling framework developed may have broad applications in delineating complex animal-environment-human interactions during zoonotic spillover and identifying opportunities for public health intervention.<br /><br />Funding: This work was supported by the Oswaldo Cruz Foundation and Secretariat of Health Surveillance, Brazilian Ministry of Health, the National Institutes of Health of the United States (grant numbers F31 AI114245, R01 AI052473, U01 AI088752, R01 TW009504 and R25 TW009338); the Wellcome Trust (102330/Z/13/Z), and by the Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB/JCB0020/2016). M.T.E is supported by an MRC doctorate studentship. F.N.S. participated in this study under a FAPESB doctorate scholarship.


2017 ◽  
Vol 9 (1) ◽  
pp. 145-162
Author(s):  
Jan Kopp ◽  
Jindřich Frajer ◽  
Marie Novotná

Abstract This study is focused on the changing of areas of water bodies in selected villages of the Pilsen Region (Czechia). We researched several different types of rural settlements and three time horizons with the help of old maps, orthophoto maps and GIS tools. To capture the influence of their location within the urban system, we chose 15 places from four categories (inner suburban area, outside suburban area, rural area, periphery rural area) depending on their distance to the core of the Pilsen agglomeration. There is no significant change in the amount of water bodies between the first reference period (1838-1839) and the second period (1957-1963) in the selected settlements. However, the third period (2013-2015) is characterized by the emergence of a large number of small water bodies - swimming pools and garden ponds. Based on the results of our research we identified the declining importance of public water bodies in some of the settlements. However, we have identified a notable prevalence of garden ponds which have a more positive ecological impact than pools. The proportion of private water bodies (covered and uncovered pools and garden ponds) in the total area of water bodies in the rural settlements in most cases is less than 20%, in the suburban settlements up to 100%. Peripheral settlements have a below-average share of these water bodies. The difference between the number of pools in different settlements is related to the proportion of newly built houses there. Although there are relatively fewer pools in rural settlements, the difference compared to the situation in suburban settlements is not pronounced due to the change in lifestyle in rural areas and the change in functions of some villages to recreational areas. Influence of pools on water consumption is dependent on the individual exchange technology of water in swimming pools. Filling of the pool before the season can overload the capacity of the local water supply.


2011 ◽  
Vol 10 (1) ◽  
pp. 31-42 ◽  
Author(s):  
James E. Amburgey ◽  
Kimberly J. Walsh ◽  
Roy R. Fielding ◽  
Michael J. Arrowood

Cryptosporidium has caused the majority of waterborne disease outbreaks in treated recreational water venues in the USA for many years running. This research project evaluated some common US swimming pool filters for removing Cryptosporidium oocysts, 5-µm diameter polystyrene microspheres, and 1-µm diameter polystyrene microspheres. A 946 L hot tub with interchangeable sand, cartridge, and precoat filters was used at room temperature for this research. Simulated pool water for each experiment was created from Charlotte, NC (USA) tap water supplemented with alkalinity, hardness, chlorine, and a mixture of artificial sweat and urine. Precoat (i.e., diatomaceous earth and perlite) filters demonstrated pathogen removal efficiencies of 2.3 to 4.4 log (or 99.4–99.996%). However, sand and cartridge filters had average Cryptosporidium removals of 0.19 log (36%) or less. The combined low filter removal efficiencies of sand and cartridge filters along with the chlorine-resistant properties of Cryptosporidium oocysts could indicate a regulatory gap warranting further attention and having significant implications on the protection of public health in recreational water facilities. The 5-µm microspheres were a good surrogate for Cryptosporidium oocysts in this study and hold promise for use in future research projects, field trials, and/or product testing on swimming pool filters.


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