Chemo-Diversity of Antibacterial Anthraquinones from the Roots of Morinda morindoides

2019 ◽  
Vol 9 (4) ◽  
pp. 256-261
Author(s):  
Jean-Luc Aboya Moroh ◽  
Yannick Fleury ◽  
Adama Coulibaly ◽  
Roger Labia ◽  
Ivan Leguérinel
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Properties ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Minimal Inhibitory Concentrations ◽  
Root Extracts ◽  
Gram Positive Bacteria ◽  
Chemical Structures ◽  
Morinda Morindoides ◽  
Susceptible Organism

Background: Morinda morindoides (Baker) (Rubiaceae) is a medicinal plant with antimicrobial properties currently used in Côte d'Ivoire and other countries. These properties have been described but most of the studies are dealing with crude extracts. Objective: The chemical structures of the bioactive compounds extracted from Morinda morindoides roots have been characterized. Methods: The root extracts were analyzed by using HPLC. Fourteen fractions were detected among which 11 compounds have been structurally identified by using a combination of 1H-NMR and 13CNMR and LC-HRMS methodologies. Results: All these compounds belong to anthraquinone family. The antibacterial activity of the eleven compounds was tested against six strains of microorganisms with ofloxacin as standard (two Gram-negative bacteria, two Gram-positive bacteria and two yeasts). The Minimal Inhibitory Concentrations recorded, varies from 8 to 128 μg / ml. Staphylococcus aureus was the most susceptible organism. Conclusion: We highlight the chemo-diversity of the antibacterial anthraquinones in the roots of Morinda morindoides.

scholarly journals An unusual case of chest pain

2020 ◽  
Vol 7 (8) ◽  
pp. 1290
Author(s):  
Saswat Subhankar ◽  
K. Madhuri ◽  
Vivek D. Alone
Keyword(s):  
Staphylococcus Aureus ◽  
Developing Countries ◽  
Chest Pain ◽  
Unusual Case ◽  
Gram Negative Bacteria ◽  
Pleural Effusions ◽  
Gram Negative ◽  
Competent Patient ◽  
E Coli ◽  
Gram Positive Bacteria

Osteomyelitis is an infection of the bones caused by pyogenic organisms. The ribs are an extremely uncommon site for osteomyelitis, occurring in less than 1% cases. The main causative organisms are Gram-positive bacteria, such as Staphylococcus aureus and Hemophilus influenzae. Gram-negative bacteria like E. coli have been rarely reported. Authors hereby present a case of an immune-competent patient who presented with an osteomyelitis of the ribs caused by the latter. In developing countries, tuberculosis is considered as the primary cause of osteomyelitis and pleural effusions. However, other organisms should also be considered in patients who present with fulminant infections.

scholarly journals Antimicrobial Activity of Gardenia jasminoides Callus and Crude Leaf Extracts for some Organic Solvents against some Pathogenic Bacteria and Yeasts

2014 ◽  
Vol 8 (3) ◽  
pp. 40-45
Author(s):  
Zina Hashem Shehab ◽  
Huda Suhail Abid ◽  
Sumaya Fadhil Hamad ◽  
Sara Haitham
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Pathogenic Bacteria ◽  
Saccharomyces Boulardii ◽  
Gram Negative Bacteria ◽  
Proteus Vulgaris ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Gardenia Jasminoides

The study was conducted to evaluate the inhibitory activity of methanol extract of Gardenia jasminoides leaves compared with leaf crude extracts for some organic solvents namely Methanol, Ethanol, Petroleum ether, Asetone and Chloroform on growth of some pathogenic bacteria and yeast, which included four gram positive isolates Staphylococcus aureus, Enterococcus faecalis, Streptococcus pyogenes and Bacillus cereus and gram negative isolates Escherichia coli, Salmonella typhi, Proteus vulgaris and Pseudomonas aeruginosa and some yeasts Candida albicans and Saccharomyces boulardii, by using well diffusion method. The inhibitory activity of extracts in the tested bacterial strains and yeasts was varied according to the type of extracting solvents and are tested microorganisms. The methanol callus extract which grown on Murashige and Skoog (MS) media by using (Naphthalen acitic acid) NAA and (Benzyle adenine) BA as growth regulator highly effective as compared to the other extracts as for inhibition of three gram positive bacteria and three gram negative bacteria,which include Staphylococcus aureus and, Proteus vulgaris, followed by acetone and ethanolic extracts which include two gram positive bacteria and two gram negative bacteria. All extracts had highly effect in growth of Candida albicans while all crude extracts didn’t show any sensitivity against Saccharomyces boulardii, and when we’d done (High Performance Liquid Chromatography) HPLC test for detection of some active compound we found Quinic acid, Iridiods glycosides and Crocin which its rate in fresh callus was higher than fresh leaves.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Espeletia nana

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Alexis Peña ◽  
Luis Rojas ◽  
Rosa Aparicio ◽  
Libia Alarcón ◽  
José Gregorio Baptista ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Gram Positive Bacteria

The essential oil of the leaves of Espeletia nana Cuatrec, obtained by hydrodistillation, was analyzed by GC-MS, which allowed the identification of 24 components, which made up 99.9% of the oil. The most abundant compounds were α-pinene (38.1%), β-pinene (17.2%), myrcene (15.0%), spathulenol (4.2%), bicyclogermacrene (4.0%), α-zingiberene (4.0%), and γhimachalene (3.7%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar disk diffusion method. Activity was observed only against Gram-positive bacteria. MIC values were determined for Staphylococcus aureus ATCC 25923(200 μg/mL) and Enterococcus faecalis ATCC 29212 (600 μg/mL).

Survival of some non-starter bacteria in naturally ripened and enzyme-accelerated Cheddar cheese

1988 ◽  
Vol 55 (4) ◽  
pp. 597-602 ◽  
Author(s):  
Lydia Bautista ◽  
Rohan G. Kroll
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Enterococcus Faecalis ◽  
Enzyme System ◽  
Cheddar Cheese ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Ripening Period ◽  
Gram Positive Bacteria

SummaryEffects of the addition of a proteinase (Neutrase 1–5S) and a peptidase (aminopeptidase DP-102) as agents for accelerating the ripening of Cheddar cheese on the survival of some non-starter bacteria (Staphylococcus aureus, Enterococcus faecalis, Escherichia coliand aSalmonellasp.) were studied throughout a 4-month ripening period. The enzymes were found to have no significant effect on the survival of the Gram-positive bacteria but some significant effects were observed, at some stages of the ripening period, with the Gram-negative bacteria in that lower levels were recovered from cheeses treated with the enzyme system.

scholarly journals Bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds

2011 ◽  
Vol 60 (1) ◽  
pp. 75-83 ◽  
Author(s):  
Svetlana A. Ermolaeva ◽  
Alexander F. Varfolomeev ◽  
Marina Yu. Chernukha ◽  
Dmitry S. Yurov ◽  
Mikhail M. Vasiliev ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Plasma Treatment ◽  
Pathogenic Bacteria ◽  
Argon Plasma ◽  
Burkholderia Cenocepacia ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Plasma Treatments

Non-thermal (low-temperature) physical plasma is under intensive study as an alternative approach to control superficial wound and skin infections when the effectiveness of chemical agents is weak due to natural pathogen or biofilm resistance. The purpose of this study was to test the individual susceptibility of pathogenic bacteria to non-thermal argon plasma and to measure the effectiveness of plasma treatments against bacteria in biofilms and on wound surfaces. Overall, Gram-negative bacteria were more susceptible to plasma treatment than Gram-positive bacteria. For the Gram-negative bacteria Pseudomonas aeruginosa, Burkholderia cenocepacia and Escherichia coli, there were no survivors among the initial 105 c.f.u. after a 5 min plasma treatment. The susceptibility of Gram-positive bacteria was species- and strain-specific. Streptococcus pyogenes was the most resistant with 17 % survival of the initial 105 c.f.u. after a 5 min plasma treatment. Staphylococcus aureus had a strain-dependent resistance with 0 and 10 % survival from 105 c.f.u. of the Sa 78 and ATCC 6538 strains, respectively. Staphylococcus epidermidis and Enterococcus faecium had medium resistance. Non-ionized argon gas was not bactericidal. Biofilms partly protected bacteria, with the efficiency of protection dependent on biofilm thickness. Bacteria in deeper biofilm layers survived better after the plasma treatment. A rat model of a superficial slash wound infected with P. aeruginosa and the plasma-sensitive Staphylococcus aureus strain Sa 78 was used to assess the efficiency of argon plasma treatment. A 10 min treatment significantly reduced bacterial loads on the wound surface. A 5-day course of daily plasma treatments eliminated P. aeruginosa from the plasma-treated animals 2 days earlier than from the control ones. A statistically significant increase in the rate of wound closure was observed in plasma-treated animals after the third day of the course. Wound healing in plasma-treated animals slowed down after the course had been completed. Overall, the results show considerable potential for non-thermal argon plasma in eliminating pathogenic bacteria from biofilms and wound surfaces.

scholarly journals Bacteriological Profile and Antimicrobial Susceptibility Patterns of Bacteria Isolated from Pus/Wound Swab Samples from Children Attending a Tertiary Care Hospital in Kathmandu, Nepal

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Salu Rai ◽  
Uday Narayan Yadav ◽  
Narayan Dutt Pant ◽  
Jaya Krishna Yakha ◽  
Prem Prasad Tripathi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Susceptibility ◽  
Gram Negative Bacteria ◽  
Wound Infections ◽  
Gram Negative ◽  
Methicillin Resistant ◽  
Gram Positive Bacteria ◽  
Wound Swab ◽  
Susceptibility Patterns

In Nepal, little is known about the microbiological profile of wound infections in children and their antimicrobial susceptibility patterns. Total of 450 pus/wound swab samples collected were cultured using standard microbiological techniques and the colonies grown were identified with the help of biochemical tests. The antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion technique. Methicillin-resistantStaphylococcus aureusisolates were detected by using cefoxitin disc and confirmed by determining minimum inhibitory concentrations (MIC) of oxacillin. 264 (59%) samples were culture positive. The highest incidence of bacterial infections was noted in the age group of less than 1 year (76%). Out of 264 growth positive samples, Gram-positive bacteria were isolated from 162 (61%) samples and Gram-negative bacteria were found in 102 (39%) samples.Staphylococcus aureus(99%) was the predominant Gram-positive bacteria isolated andPseudomonas aeruginosa(44%) was predominant Gram-negative bacteria. About 19% ofS. aureusisolates were found to be methicillin-resistant MIC of oxacillin ranging from 4 μg/mL to 128 μg/mL. Among the children of Nepal, those of age less than 1 year were at higher risk of wound infections by bacteria.Staphylococcus aureusfollowed byPseudomonas aeruginosawere the most common bacteria causing wound infections in children.

scholarly journals Synthesis of cyclotetrapeptide analogues of c-PLAI and evaluation of their antimicrobial properties

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Rani Maharani ◽  
Orin Inggriani Napitupulu ◽  
Jelang M. Dirgantara ◽  
Ace Tatang Hidayat ◽  
Dadan Sumiarsa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Properties ◽  
Trichophyton Mentagrophytes ◽  
Solution Phase ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Aromatic Residues ◽  
Gram Positive Bacteria ◽  
Lysine Residues ◽  
Bacteria And Fungi

Antimicrobial peptides (AMPs) are interesting compounds owing to their ability to kill several pathogens. In order to identify new AMPs, c-PLAI analogues were synthesized and evaluated together with their linear precursors for their antimicrobial properties against two Gram-positive bacteria ( Staphylococcus aureus and Bacillus cereus ), two Gram-negative bacteria ( Escherichia coli and Klebsiella pneumoniae ), and two fungal strains ( Candida albicans and Trichophyton mentagrophytes ). The new c-PLAI analogues were prepared through a combination of solid- and solution-phase syntheses, as previously employed for the synthesis of c-PLAI. The antimicrobial activity tests showed that the synthetic parent peptide c-PLAI was inactive or weakly active towards the bioindicators employed in the assay. The tests also indicated that cyclic c-PLAI analogues possessed enhanced antimicrobial properties against most of the bacteria and fungi tested. Furthermore, this study revealed that analogues containing cationic lysine residues displayed the highest activity towards most bioindicators. A combination of lysine and aromatic residues yielded analogues with broad-spectrum antimicrobial properties.

The role of biosynthesized silver nanoparticles in antimicrobial mechanisms

2021 ◽  
Vol 22 ◽  
Author(s):  
Bianca PizzornoBackx ◽  
Mayara Santana dos Santos ◽  
Otávio Augusto Leitão dos Santos ◽  
Sérgio Antunes Filho
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antimicrobial Properties ◽  
New Drugs ◽  
Gram Negative Bacteria ◽  
New Materials ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Emergence Of Resistance

: Nanotechnology is an area of science able to develop new materials. The relation between nanotechnology and microbiology is essential for the development of new drugs and vaccines. The main advantage of blend in both areas is to associate the latest technology to obtain new ways to solve problems related to microorganisms. This review seeks to investigate nanoparticle formation's antimicrobial properties, primarily when connected to the green synthesis of silver nanoparticles. The development of new sustainable methods for nanoparticle production has been instrumental in designing alternative, non-toxic, energy-friendly, and environmentally friendly routes. In this sense, it is necessary to study silver nanoparticles' green synthesis concerning their antimicrobial properties. Antimicrobial mechanisms of silver nanoparticles demonstrate efficiency to gram-positive bacteria, gram-negative bacteria, fungi, viruses, and parasites. However, attention is needed with the emergence of resistance to these antimicrobials. This article seeks to relate the parameters of green silver-based nanosystems with the efficiency of antimicrobial activity.

scholarly journals Novel 4-Thiazolidinone Derivatives as Anti-Infective Agents: Synthesis, Characterization, and Antimicrobial Evaluation

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Amit Gupta ◽  
Rajendra Singh ◽  
Pankaj K. Sonar ◽  
Shailendra K. Saraf
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Pseudomonas Fluorescens ◽  
Broad Spectrum ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Spectral Techniques ◽  
Fungal Strains ◽  
Gram Positive Bacteria ◽  
Antimicrobial Evaluation

A series of new 4-thiazolidinone derivatives was synthesized, characterized by spectral techniques, and screened for antimicrobial activity. All the compounds were evaluated against five Gram-positive bacteria, two Gram-negative bacteria, and two fungi, at concentrations of 50, 100, 200, 400, 800, and 1600 µg/mL, respectively. Minimum inhibitory concentrations of all the compounds were also determined and were found to be in the range of 100–400 µg/mL. All the compounds showed moderate-to-good antimicrobial activity. Compounds4a[2-(4-fluoro-phenyl)-3-(4-methyl-5,6,7,8-tetrahydro-quinazolin-2-yl)-thiazolidin-4-one] and4e[3-(4,6-dimethyl-pyrimidin-2-yl)-2-(2-methoxy-phenyl)-thiazolidin-4-one] were the most potent compounds of the series, exhibiting marked antimicrobial activity againstPseudomonas fluorescens,Staphylococcus aureus,and the fungal strains. Thus, on the basis of results obtained, it may be concluded that synthesized compounds exhibit a broad spectrum of antimicrobial activity.

scholarly journals Paper modified with ZnO nanorods – antimicrobial studies

2012 ◽  
Vol 3 ◽  
pp. 684-691 ◽  
Author(s):  
Mayuree Jaisai ◽  
Sunandan Baruah ◽  
Joydeep Dutta
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Aspergillus Niger ◽  
Zno Nanorods ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Tissue Paper ◽  
Gram Positive Bacteria ◽  
Lighting Conditions

Paper with antimicrobial properties was developed through in situ growth of ZnO nanorods. The targeted application for this type of paper is in health centers as wallpaper, writing paper, facemasks, tissue paper, etc. The paper was tested on three model microbes, Gram-positive bacteriaStaphylococcus aureus,Gram-negative bacteriaEscherichia coliand common airborne fungusAspergillus niger. No viable bacterial colonies or fungal spores could be detected in the areas surrounding test samples of the antimicrobial paper. Gram-negative bacteriaEscherichia coliwere found to be inhibited in an area that is 239% and 163% the area of the paper sample under different room lighting conditions, i.e., halogen and fluorescent lamp illumination, respectively. For Gram-positive bacteriaStaphylococcus aureusthe zones of inhibition surrounding the paper samples are 102% and 70%, and forAspergillus niger, 224% and 183% of the sample area, under similar lighting conditions.

Sign in / Sign up

Export Citation Format

Share Document