scholarly journals Isolation and identification of chemical compounds from stem and roots of Sesbania sesban

2017 ◽  
Vol 6 (10) ◽  
pp. 1718 ◽  
Author(s):  
Baljinder Singh ◽  
Rajiv Sharma ◽  
Kalia A.N. ◽  
Vijender Kumar
Keyword(s):  
Column Chromatography ◽  
Spectroscopic Analysis ◽  
Chemical Compounds ◽  
Sesbania Sesban ◽  
Phytochemical Screening ◽  
Isolation And Identification ◽  
Butanol Extract ◽  
Rheumatic Conditions ◽  
Identification And Characterization

Sesbania sesban is a small perennial tree with woody stems, yellow flowers which is commonly known as Rawasan, family Leguminosae, is one of the restorative plant for inflammatory rheumatic conditions. This study on identification and characterization of isolated chemical compounds from n-butanol extract of root and stems of the plant S. sesban. This research is continued from previous research which is done by preliminary phytochemical screening and biological evaluations of extracts. The n-butanol extract was selected for compound isolation with column chromatography and identified by UV-VIS Spectrophotometer, FTIR, Mass and NMR Spectroscopic analysis. The results showed the isolated compounds identified as β-Sitosterol and Kaempferol.

scholarly journals Karakterisasi Fraksi Aktif Antioksidan dari Ekstrak Etanol Biji Kemangi (Ocimum Basilicum L.)

2015 ◽  
pp. 39-49
Author(s):  
Dede Sukandar ◽  
Sandra Hermanto ◽  
Eka Rizki Amelia ◽  
Chitta Putri Novianti
Keyword(s):  
Ethyl Acetate ◽  
Column Chromatography ◽  
Ethanol Extract ◽  
Ocimum Basilicum ◽  
Total Phenolic ◽  
Solvent Fractionation ◽  
Butanol Extract ◽  
Dpph Method ◽  
Ic50 Values

Characterization of antioxidant compounds from the seeds of basil (Ocimum basilicum L.) has been done. Extraction is done by maceration method using ethanol solvent, fractionation by TLC and column chromatography, antioxidants test using DPPH method, and characterization of antioxidant compound using GCMS. Ethanol extract and results of fractionation ethanol extract of basil seeds using n-hexane, ethyl acetate, n-butanol and methanol-water extracts show that n-butanol extract has the highest antioxidant activity with IC50 values ​​of 41.90 ppm. Results of column chromatography n-butanol extract using n-hexane : ethyl acetate (1:9) as mobile phase yielded 5 fractions with fraction 4 (F4 isolate) has dominant stain of active antioxidants after being sprayed DPPH reagent, it had IC50 values ​​of 39,70 ppm and total phenolic content of 0,003 mg/g. Isolate F4 suspected contains two active compounds as antioxidant which is terpenoid and phenolic compound group, namely squalene and 1,4-di-tert-buthyl-phenol identified by GCMS.DOI :http://dx.doi.org/10.15408/jkv.v0i0.3598

scholarly journals Isolasi Senyawa Kimia Stigmastan-3,5-Diena Yang Mempunyai Daya Toksik Dari Daun Ekaliptus (Eucalyptus Deglupta Blume.)

2017 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Sari Setianingsih ◽  
Rudi Kartika ◽  
Partomuan Simanjuntak
Keyword(s):  
Ethyl Acetate ◽  
Toxicity Test ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Test Results ◽  
Phytochemical Screening ◽  
Isolation And Identification ◽  
Lc50 Value ◽  
Ms Analysis

This study was started by extraction of Eucalyptus deglupta Blume. Using organic solvent   (n-hexane, ethyl acetate, ethanol and water) followed by phytochemical screening and toxicity test using Brine Shrimp Lethality Test (BSLT) method. Isolation and identification of chemical compounds contained in the fraction were done by column chromatography and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Phytochemical screening revealed the presence of alkaloids, flavonoids, steroids and phenolics in the extract. Toxicity test results showed that the ethyl acetate extract was potentially active with LC50 value of  617.95 ppm. The extract was continued to isolation stage and gave fraction EKEA-3.1 with LC50 value of 2759.93 ppm. Identification of chemical compounds in EKEA-3.1 with KG-MS analysis showed that EKEA-3.1 was suspected to be Stigmastan-3,5-diene.

scholarly journals UJI EFEKTIVITAS DAN IDENTIFIKASI SENYAWA AKTIF EKSTRAK DAUN SIRSAK SEBAGAI PESTISIDA NABATI TERHADAP MORTALITAS KUTU DAUN PERSIK (Myzus persicae Sulz) PADA TANAMAN CABAI MERAH (Capsipcum annum L.)

Jurnal Kimia ◽  
2016 ◽  
Author(s):  
Ni Made Dwi Desiyanti ◽  
I Made Dira Swantara ◽  
I Putu Sudiarta
Keyword(s):  
Ir Spectra ◽  
Silica Gel ◽  
Column Chromatography ◽  
Myzus Persicae ◽  
Mobile Phase ◽  
Ethanol Extract ◽  
Annona Muricata ◽  
Isolation And Identification ◽  
Butanol Extract ◽  
Metabolite Extraction

The study of isolation and identification of the active compounds of soursop (Annona muricata L.) leave extract were conducted . The metabolite extraction was conducted using maceration method with 96 % ethanol. The ethanol extract was used to test the mortility of aphid (Myzus persicae S.), with LC50 of 100 ppm. The n-hexane, chloroform, and n-buthanol were used to fractionate the ethanol extract. The mortality test of those three extracts showed the LC50 of 545.12 ppm, 136.26 ppm and 117.73 ppm, respectively. The n-butanol extract was separated using silica gel column chromatography with chloroform: ethanol: water (5:4:1), as the mobile phase. The fractions resulted were FI, FII, FIII, FIV and FV. The mortality test indicated that FII was the best with LC50 of 596.48 ppm. The FII was purified using silica gel column chromatography, resulting three fractions (FII.1, FII.2 and FII.3).  The mortality test of those fractions indicated that FII.2 showed the best result with LC50 of 601.17 ppm. The UV-Vis and IR spectra showed that FII.2 fraction contained flavonoides under the flavonon family.

scholarly journals Isolation and Characterization of Sclerienone C from Scleria Striatinux

2016 ◽  
Vol 11 (1) ◽  
pp. 1934578X1601100
Author(s):  
Kennedy D. Nyongbela ◽  
Felix L. Makolo ◽  
Thomas R. Hoye ◽  
Simon MN Efange
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Structure Elucidation ◽  
Spectroscopic Analysis ◽  
Methylene Chloride ◽  
Isolation And Purification ◽  
Isolation And Characterization ◽  
Combination Of Methods

Herein, we report the isolation and characterization of sclerienone C, a novel sesquiterpene isolated from the methylene chloride/methanol (1:1) extract of Scleria striatinux that we have deduced to have structure 1. This medicinal spice of Cameroon has been shown to display antimicrobial and antiplasmodial activities. The isolation and purification involved a combination of methods including silica gel column chromatography, Sephadex LH-20, and semi-prep HPLC separations. Structure elucidation was carried-out by means of spectroscopic analysis and comparison with previously isolated sesquiterpene derivatives from the plant.

scholarly journals PHYTOCHEMICAL EVALUATION OF MONOCOT GRASS KYLLINGA TRICEPS ROTTB.

2018 ◽  
Vol 8 (5) ◽  
pp. 204-208
Author(s):  
Amit Upadhyay ◽  
Suman Jain ◽  
Neetu Bhalla ◽  
Kannika Arora
Keyword(s):  
Medicinal Plant ◽  
Plant Extract ◽  
Perennial Herb ◽  
Correct Identification ◽  
Phytochemical Screening ◽  
Liver Disorders ◽  
The Family ◽  
Original Plant ◽  
Identification And Characterization

Kyllinga triceps rottb. A monocot perennial herb found in various parts of India is traditionally used in vitiated conditions pitta and vata, hyperdipsia, fever, liver disorders, verminosis, cough, splenopathy, diabetes and dermatitis. The aim of the present study was to evaluate the phytochemical and pharmacognostical study of ignored ayurvedic medicinal herb kyllinga triceps rottb. The plant is monocot grass belongs to the family cyperaceae, commonly used in various ayurvedic preparation’s and called musta. In various ayurvedic texts it is also known as nirvishi. Many species of family cyperaceae resembles the original drug thus the present study will help in identification and collection of original plant. The study includes identification and characterization of chemical component and preliminary phytochemical screening of the plant extract. The generated information of the present study will provide data which are helpful in the correct identification and authentication of medicinal plant kyllinga triceps rottb. and may help in prevention of its adulteration. Keywords: Kyllinga triceps rottb., phytochemical, cyperaceae, adulteration, ayurevedic.

scholarly journals ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF SALMONELLA SEROVARS FROM DIARRHOEIC STOOL SAMPLES OF HUMAN

2012 ◽  
Vol 9 (1) ◽  
pp. 85-93 ◽  
Author(s):  
MK Nesa ◽  
MSR Khan ◽  
M Alam
Keyword(s):  
First Line ◽  
Isolation And Identification ◽  
First Line Therapy ◽  
Antimicrobial Sensitivity ◽  
Highly Sensitive ◽  
Stool Samples ◽  
Salmonella Serovars ◽  
Identification And Characterization ◽  
Human Stool

    The present study aimed at isolation and identification of Salmonella serovars from human stool and characterization of the isolated serovars using biochemical, serological, molecular and antimicrobial sensitivity techniques. A total of 25 samples were collected of which 16% were positive to Salmonella serovars. All the culturally positive isolates fermented dextrose, maltose and mannitol with the peoduction of acid and gas but not fermented sucrose and lactose. The same isolates showed Indole and V-P tests negative but M-R test positive. All the culturally and biochemically positive Salmonella serovars showed agglutination with poly ‘O’ and poly ‘H’ antisera. The antimicrobial susceptibility testing showed that the isolated Salmonella serovars were highly sensitive to ciprofloxacin and moderately sensitive to chloramphenicol, kanamycin, cotrimoxazol and nalidixic acid. However, the positive isolates were resistant to erythromycin. The present study indicates that ciprofloxacin can be used as a first line therapy for the treatment of Salmonella gastroenteritis.DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11218Bangl. J. Vet. Med. (2011). 9(1): 85-93

Isolation and Characterization of Flavonoids of the Ethanolic extracts of stems of Mimosa hamata (Willd.) by Chromatographic techniques

2021 ◽  
pp. 4602-4608
Author(s):  
N.I. Khan ◽  
B.C. Hatapakki ◽  
A.M. Tamboli
Keyword(s):  
Column Chromatography ◽  
Bioactive Compound ◽  
Solvent System ◽  
Chromatographic Techniques ◽  
Isolation And Characterization ◽  
Ethanolic Extracts ◽  
Chloroform Methanol ◽  
Identification And Characterization ◽  
Mass Spectroscopic Study

Since there are many bioactive compounds present in plant material containing various multi-component mixtures, their separation and determination is important to identify the active phytoconstituents responsible for pharmacological activity. Practically most of them have to be separated by column chromatographic techniques. The present study deals with the identification and characterization of bioactive principles from the stems of Mimosa hamata. The isolated fractions from the ethanolic extracts of stems of Mimosa hamata was carried out by column chromatography. For separation of a bioactive compound, the solvent system tried for column chromatography was Chloroform: Methanol in various ratios like 90:10, 80:20, 70:30, and 60:40 amongst these we could separate the first fraction at 80:20 and second fraction at 70:30. Two flavonoids compounds were isolated from the ethanolic extracts of stems of the medicinal plant Mimosa hamata (Willd). Based on chemical and spectral analyses their structures were elucidated as Quercetin and Cirsimaritin. From the above study it reveals the presence of flavonoids in the ethanolic extracts of stems of Mimosa hamata (Willd.) was isolated using column chromatography further subjected to characterization of isolated compounds including UV, IR, NMR and Mass spectroscopic study for elucidating the structure of the two separated compounds. The interpreted data concluded that, both isolated compounds are flavonoids i.e. Cirsimaritin and Quercetin. The pharmacological effect of Mimosa hamata stems may be due to the presence of its phytoconstituents flavonoids.

scholarly journals Identification and characterization of chemical compounds in different crude extracts from leaves of Omani neem

2013 ◽  
Vol 7 (4) ◽  
pp. 181-188 ◽  
Author(s):  
Mohammad A. Hossain ◽  
Wafa A.S. Al-Toubi ◽  
Afaf M. Weli ◽  
Qasim A. Al-Riyami ◽  
Jamal N. Al-Sabahi
Keyword(s):  
Chemical Compounds ◽  
Crude Extracts ◽  
Identification And Characterization

Characterization of Chemical Impurities in Glutaraldehyde

1975 ◽  
Vol 33 ◽  
pp. 620-621
Author(s):  
B. J. Grenon ◽  
A. J. Tousimis
Keyword(s):  
Glutaric Acid ◽  
Spectroscopic Analysis ◽  
Aldol Condensation ◽  
Condensation Product ◽  
Amorphous Solid ◽  
Water Soluble ◽  
Impurity Component ◽  
Chemical Impurities ◽  
Soluble Liquid

Ever since the introduction of glutaraldehyde as a fixative in electron microscopy of biological specimens, the identification of impurities and consequently their effects on biologic ultrastructure have been under investigation. Several reports postulate that the impurities of glutaraldehyde, used as a fixative, are glutaric acid, glutaraldehyde polymer, acrolein and glutaraldoxime.Analysis of commercially available biological or technical grade glutaraldehyde revealed two major impurity components, none of which has been reported. The first compound is a colorless, water-soluble liquid with a boiling point of 42°C at 16 mm. Utilizing Nuclear Magnetic Resonance (NMR) spectroscopic analysis, this compound has been identified to be — dihydro-2-ethoxy 2H-pyran. This impurity component of the glutaraldehyde biological or technical grades has an UV absorption peak at 235nm. The second compound is a white amorphous solid which is insoluble in water and has a melting point of 80-82°C. Initial chemical analysis indicates that this compound is an aldol condensation product(s) of glutaraldehyde.

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