Initial Validation of a Replicated Field-scale Denitrifying Bioreactor Facility in a Boreal Environment

2020 ◽  
Vol 02 (02) ◽  
pp. 1-1
Author(s):  
Lordwin Girish Kumar Jeyakumar ◽  
◽  
David B. McKenzie ◽  
Laura E. Christianson ◽  
Evan Derdall ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Retention Time ◽  
Removal Rate ◽  
Internal Flow ◽  
Drainage Water ◽  
Chloride Salt ◽  
Field Scale ◽  
Reactive Medium ◽  
N Removal ◽  
Boreal Environment

Denitrifying bioreactor technology, where a solid carbon source (woodchips) acts as a reactive medium to intercept agricultural tile drainage water, has been successfully used to convert N (NO3-) to di-nitrogen (N2) gas. Four replicated field-scale (24 m long x 3 m wide x 1 m deep), bioreactors were built and operated at the St. John’s Research and Development Centre and were successful at removing a notable amount of nitrate (N) from agricultural subsurface drainage water. The objective of this study was to investigate the internal flow dynamics of one of these field-scale bioreactors as a proxy for the others. The hydraulic conditions in the bioreactor system developed differently than expected; asymmetric flow rates led to long average hydraulic retention time (HRT) and a highly dispersed residence time distribution, which was revealed by a sodium chloride tracer test. To measure the internal flow a known amount of sodium chloride (salt) was added to water before it entered the bioreactor and samples were collected in 30 minutes intervals. The temperature of water samples taken from the inlet, outlet, and sample ports ranged from 14.5 to 18.4°C With a N removal of 62 to 66% the bioreactor proved at the same time to be very effective under the boreal environment of Newfoundland and Labrador (NL). Mass removal rate (MRR) was calculated to evaluate the performance of woodchip bioreactor. The average MRR was 3.87 gm-3day-1 and the highest was 7.19 gm-3day-1 respectively. The theoretical retention time was calculated to be approximately 10.64 h based on the active flow volume, the length and depth of the system. In comparison the observed retention was 18.18 h

scholarly journals In Situ Water Quality Improvement Mechanism (Nitrogen Removal) by Water-Lifting Aerators in a Drinking Water Reservoir

Water ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 1051 ◽  
Author(s):  
Zizhen Zhou ◽  
Tinglin Huang ◽  
Weijin Gong ◽  
Yang Li ◽  
Yue Liu ◽  
...  
Keyword(s):  
Drinking Water ◽  
Nitrogen Removal ◽  
High Throughput Sequencing ◽  
Removal Rate ◽  
Water Reservoir ◽  
Control Area ◽  
Nitrite Accumulation ◽  
Field Scale ◽  
Drinking Water Reservoir ◽  
N Removal

A field scale experiment was performed to explore the nitrogen removal performance of the water and surface sediment in a deep canyon-shaped drinking water reservoir by operating WLAs (water-lifting aerators). Nitrogen removal performance was achieved by increasing the densities and N-removal genes (nirK and nirS) of indigenous aerobic denitrifiers. After the operation of WLAs, the total nitrogen removal rate reached 29.1 ± 0.8% in the enhanced area. Ammonia and nitrate concentrations were reduced by 72.5 ± 2.5% and 40.5 ± 2.1%, respectively. No nitrite accumulation was observed. Biolog results showed improvement of carbon metabolism and carbon source utilization of microbes in the enhanced area. Miseq high-throughput sequencing indicated that the denitrifying bacteria percentage was also higher in the enhanced area than that in the control area. Microbial communities had changed between the enhanced and control areas. Thus, nitrogen removal through enhanced indigenous aerobic denitrifiers by the operation of WLAs was feasible and successful at the field scale.

Influence of C/N Ratio on SND and Microbiological Analysis in Catching Bed Biofilm Reactor Using Acrylic Resin Fiber as Carrier Materials in Civil Engineering

2012 ◽  
Vol 568 ◽  
pp. 89-93
Author(s):  
Yan Zhang ◽  
Zheng Yang Yang ◽  
Li Li Wang ◽  
Xu Ying Zhao ◽  
Huan Guang Liu ◽  
...  
Keyword(s):  
Civil Engineering ◽  
Removal Rate ◽  
Acrylic Resin ◽  
Biofilm Reactor ◽  
Easy Access ◽  
Fish Analysis ◽  
Microbiological Analysis ◽  
Ammonia Oxidizing Bacteria ◽  
N Removal ◽  
Carrier Materials

In this study, effect of C/N ratio on denitrification were investigated using four sets of parallel catching bed reactors, which were using acrylic resin fiber (ARF) as carrier materials. The results indicate that this process which was used in wastewater treatment of civil engineering can get better COD and nitrogen removing performance. NH4+-N removal rate reduced with the increasing of C/N ratio, and the average removal rate of COD and the total nitrogen (TN) increased when C/N ratio is increased. When C/N ratio exceeded 12, TN removal rate has no obvious growing. Meanwhile, fluorescent in situ hybridization (FISH) analysis indicated that the biomass in the biofilm were much richer than which in the suspension, and the ammonia oxidizing bacteria have a easy access to be dominant bacterial community in lower C/N ratio.

Performance of intermittent aeration reactor on NH4-N removal from groundwater resources

2010 ◽  
Vol 61 (12) ◽  
pp. 3061-3069 ◽  
Author(s):  
W. Khanitchaidecha ◽  
T. Nakamura ◽  
T. Sumino ◽  
F. Kazama
Keyword(s):  
Removal Rate ◽  
Groundwater Resources ◽  
Ammonium Nitrogen ◽  
Total Carbon ◽  
Intermittent Aeration ◽  
Reactor Performance ◽  
Total Carbon Content ◽  
Term Operation ◽  
N Removal ◽  
Synthetic Groundwater

To study the effect of intermittent aeration period on ammonium–nitrogen (NH4-N) removal from groundwater resources, synthetic groundwater was prepared and three reactors were operated under different conditions – “reactor A” under continuous aeration, “reactor B” under 6 h intermittent aeration, and “reactor C” under 2 h intermittent aeration. To facilitate denitrification simultaneously with nitrification, “acetate” was added as an external carbon source with step-wise increase from 0.5 to 1.5 C/N ratio, where C stands for total carbon content in the system, and N for NH4-N concentration in the synthetic groundwater. Results show that complete NH4-N removal was obtained in “reactor B” and “reactor C” at 1.3 and 1.5 C/N ratio respectively; and partial NH4-N removal in “reactor A”. These results suggest that intermittent aeration at longer interval could enhance the reactor performance on NH4-N removal in terms of efficiency and low external carbon requirement. Because of consumption of internal carbon by the process, less amount of external carbon is required. Further increase in carbon in a form of acetate (1.5 to 2.5 C/N ratios) increases removal rate (represented by reaction rate coefficient (k) of kinetic equation) as well as occurrence of free cells. It suggests that the operating condition at reactor B with 1.3 C/N ratio is more appropriate for long-term operation at a pilot-scale.

scholarly journals Nitrogen-metabolising Microorganism Analysis in Rapid Sand Filters From Drinking Water Treatment Plant by Culturing and Metagenomics

2021 ◽  
Author(s):  
Qihui Gu ◽  
Jun Ma ◽  
Jumei Zhang ◽  
Weipeng Guo ◽  
Huiqing Wu ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
Removal Rate ◽  
Treatment Plant ◽  
Drinking Water Treatment ◽  
Water Treatment Plant ◽  
Amplicon Sequencing ◽  
Metabolic Model ◽  
Rrna Gene ◽  
N Removal

Abstract Sand filter (SFs) are common treatment processes for nitrogen pollutants removal in drinking water treatment plants (DWTPs). However, the mechanisms on the nitrogen-cycling role of SFs are still unclear. In this study, 16S rRNA gene amplicon sequencing was used to characterise the diversity and composition of the bacterial community in SFs from DWTPs. Additionally, metagenomics approach was used to determine the functional microorganisms involved in nitrogen cycle in SFs. Our results showed that Proteobacteria, Acidobacteria, Nitrospirae, and Chloroflexi dominated in SFs. Subsequently, 85 high-quality metagenome-assembled genomes (MAGs) were retrieved from metagenome datasets of selected SFs involving nitrification, assimilatory nitrogen reduction, and denitrification processes. Read mapping to reference genomes of Nitrospira and the phylogenetic tree of the ammonia monooxygenase subunit A gene, amoA, suggested that Nitrospira is abundantly found in SFs. Furthermore, according to their genetic content, a nitrogen metabolic model in SFs was proposed using representative MAGs and pure culture isolates. Quantitative real-time polymerase chain reaction (PCR) showed that ammonia-oxidising bacteria (AOB) and archaea (AOA), and complete ammonia oxidisers (comammox) were ubiquitous in the SFs, with the abundance of comammox being higher than that of AOA and AOB. Moreover, we identified a bacterial strain with a high NO3-N removal rate as Pseudomonas sp., which could be applied in the bioremediation of micro-polluted drinking water sources. Our study provides insights into functional nitrogen-metabolising microbes in SFs of DWTPs.

Nutrient Removal from Polluted River Water by Combining Vertical and Horizontal Subsurface Flow Constructed Wetlands

2013 ◽  
Vol 663 ◽  
pp. 1029-1032 ◽  
Author(s):  
Cheng Xin Qin ◽  
Gang He ◽  
Yu Huan Duan ◽  
Xiao Ping Pang ◽  
Zong Lian She
Keyword(s):  
River Water ◽  
Removal Rate ◽  
Subsurface Flow ◽  
Ammonia Removal ◽  
Polluted River ◽  
Main Layer ◽  
Second Stage ◽  
N Removal ◽  
Different Depths ◽  
Horizontal Subsurface Flow

A lab-scale hybrid constructed wetland system was constructed to purify polluted river water. The system was composed of a first stage of the vertical subsurface flow filter, followed by a second stage of horizontal subsurface flow bed. Both beds used furnace slag with a size of 4-60 mm for the main layer. The system was continuously fed. Different depths of unsaturated layer (0 cm, 15 cm and 30 cm) in vertical filter were tested. The unsaturated layer of 30 cm in vertical filter presented the most effective ammonia removal of 89.1%, while lowest NO3--N removal rate of 74.1% for the system. High TN removal efficiencies (77.3%-81.0%) could be observed during operation of three depths. The removals of COD and TP were in the range of 97.1%-98.4% and 76.4%-88.9%, respectively.

scholarly journals Upflow packed bed Anammox reactor used in two-stage deammonification of sludge digester effluent

2018 ◽  
Vol 78 (9) ◽  
pp. 1843-1851 ◽  
Author(s):  
İ. Çelen-Erdem ◽  
E. S. Kurt ◽  
B. Bozçelik ◽  
B. Çallı
Keyword(s):  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Municipal Wastewater ◽  
Removal Rate ◽  
Treatment Plant ◽  
Packed Bed ◽  
Municipal Wastewater Treatment ◽  
Two Stage ◽  
N Removal ◽  
Total Nitrogen Removal

Abstract The sludge digester effluent taken from a full scale municipal wastewater treatment plant (WWTP) in Istanbul, Turkey, was successfully deammonified using a laboratory scale two-stage partial nitritation (PN)/Anammox (A) process and a maximum nitrogen removal rate of 1.02 kg N/m3/d was achieved. In the PN reactor, 56.8 ± 4% of the influent NH4-N was oxidized to NO2-N and the effluent nitrate concentration was kept below 1 mg/L with 0.5–0.7 mg/L of dissolved oxygen and pH of 7.12 ± 12 at 24 ± 4°C. The effluent of the PN reactor was fed to an upflow packed bed Anammox reactor where high removal efficiency was achieved with NO2-N:NH4-N and NO3-N:NH4-N ratios of 1.32 ± 0.19:1 and 0.22 ± 0.10:1, respectively. The results show that NH4-N removal efficiency up to 98.7 ± 2.4% and total nitrogen removal of 87.7 ± 6.5% were achieved.

Experience from start-ups of the first ANITA Mox Plants

2013 ◽  
Vol 67 (12) ◽  
pp. 2677-2684 ◽  
Author(s):  
M. Christensson ◽  
S. Ekström ◽  
A. Andersson Chan ◽  
E. Le Vaillant ◽  
R. Lemaire
Keyword(s):  
Nitrogen Removal ◽  
Removal Rate ◽  
Treatment Plant ◽  
Ammonia Removal ◽  
Nitrogen Loading ◽  
Biofilm Reactor ◽  
Process Conditions ◽  
N2o Emissions ◽  
N Removal ◽  
Start Up

ANITA™ Mox is a new one-stage deammonification Moving-Bed Biofilm Reactor (MBBR) developed for partial nitrification to nitrite and autotrophic N-removal from N-rich effluents. This deammonification process offers many advantages such as dramatically reduced oxygen requirements, no chemical oxygen demand requirement, lower sludge production, no pre-treatment or requirement of chemicals and thereby being an energy and cost efficient nitrogen removal process. An innovative seeding strategy, the ‘BioFarm concept’, has been developed in order to decrease the start-up time of new ANITA Mox installations. New ANITA Mox installations are started with typically 3–15% of the added carriers being from the ‘BioFarm’, with already established anammox biofilm, the rest being new carriers. The first ANITA Mox plant, started up in 2010 at Sjölunda wastewater treatment plant (WWTP) in Malmö, Sweden, proved this seeding concept, reaching an ammonium removal rate of 1.2 kgN/m3 d and approximately 90% ammonia removal within 4 months from start-up. This first ANITA Mox plant is also the BioFarm used for forthcoming installations. Typical features of this first installation were low energy consumption, 1.5 kW/NH4-N-removed, low N2O emissions, <1% of the reduced nitrogen and a very stable and robust process towards variations in loads and process conditions. The second ANITA Mox plant, started up at Sundets WWTP in Växjö, Sweden, reached full capacity with more than 90% ammonia removal within 2 months from start-up. By applying a nitrogen loading strategy to the reactor that matches the capacity of the seeding carriers, more than 80% nitrogen removal could be obtained throughout the start-up period.

Effects of sodium chloride and betaine on hydration status of lambs at slaughter

2008 ◽  
Vol 48 (9) ◽  
pp. 1194 ◽  
Author(s):  
K. L. Pearce ◽  
D. G. Masters ◽  
R. H. Jacob ◽  
D. L. Hopkins ◽  
D. W. Pethick
Keyword(s):  
Sodium Chloride ◽  
Carcass Characteristics ◽  
Carcass Weight ◽  
Urine Specific Gravity ◽  
Hydration Status ◽  
Chloride Salt ◽  
Feeding Period ◽  
High Salt Diet ◽  
Access To Water ◽  
The Difference

Under commercial conditions in Australia, lambs are often dehydrated at slaughter despite access to water at the abattoir. Dehydration results in reduced fluid content of meat, which causes liveweight and carcass weight loss. Two experiments evaluated hydration status and carcass characteristics in lambs fed increased levels of sodium chloride (salt) and/or betaine before slaughter. Both experiments were 2 × 2 factorial designs with two levels of salt added [0 and 50 g/kg dry matter (DM)] and two levels of betaine added (0 and 6.7 g/kg DM) to the diets. The treatment diets were introduced to sheep over a 7–8-day period and then fed at 1.5 kg/day for the following 7 days. In experiment 1, 96 Poll Dorset–Merino cross wether lambs (45.9 ± 0.17 kg) were fed in individual pens and in experiment 2, 204 Merino wether lambs (43.1 ± 0.35 kg) were fed in group pens. In the first experiment, water and feed were withheld from the end of the treatment feeding period until slaughter 48 h later. In the second experiment, feed and water were withheld for the first 24 h after treatment but water was available for the next 24 h immediately before slaughter. In both experiments, consumption of the high salt diet decreased urine specific gravity and osmolarity at the end of the feeding period and after 24 h without access to water. This indicates an improved hydration status at this time. However, the difference in hydration status related to feeding salt declined during the 48-h lairage period and there were no significant differences in hot carcass weight or muscle DM among groups at slaughter. It could be concluded from this result that the addition of salt has little commercial value; however, this may not be the case. The lambs in experiment 2 did not behave according to industry expectations. They all drank water in the last 24 h in lairage and none of the groups showed severe dehydration. Further research is justified to determine if the lambs fed the diets with no added salt drunk in lairage as a learned response from the lambs fed salt and, if so, whether this can be developed into a commercial strategy. Feeding betaine did not result in any changes in hydration status or carcass characteristics.

Ammonium removal in constructed wetlands with recirculating subsurface flow: removal rates and mechanisms

1995 ◽  
Vol 32 (3) ◽  
pp. 193-202 ◽  
Author(s):  
F. J. Sikora ◽  
Tong Zhu ◽  
L. L. Behrends ◽  
S. L. Steinberg ◽  
H. S. Coonrod
Keyword(s):  
Constructed Wetlands ◽  
Constructed Wetland ◽  
Removal Rate ◽  
N Uptake ◽  
Subsurface Flow ◽  
N Removal ◽  
Air Water Interface ◽  
Scirpus Cyperinus ◽  
Macrophyte Growth ◽  
Discernible Difference

From June 1993 through February 1994, the removal of NH4-N was evaluated in constructed wetlands at the TVA constructed wetland research facility in Muscle Shoals, AL. The objectives were to determine rates for NH4-N removal and speculate on potential mechanisms for removal. Nine constructed wetland cells were used with approximate dimensions of 9.1 × 6.1 × 0.6 m3 and a recirculating subsurface flow system in a gravel base. Treatments consisted of an unplanted (WO=control) and two polycultural planting schemes (P1=Scirpus acutus, Phragmites communis and Phalaris arundinacea; P2=Typha sp., Scirpus atrovirens georgianus and Scirpus cyperinus) replicated 3 times. Salt solutions were added and recirculated in each cell resulting in initial concentrations of 50 and 300 mg l−1 of NH4-N and COD, respectively, when fully diluted with wetland water. Salts were added to wetlands approximately every 6 weeks with the first addition on June 1, 1993 and the last addition on February 9, 1994 for a total of 6 time periods (times I, II, III, IV, V and VI). The COD of the waters was removed at rates ranging from 5.5 to 10 g/m2/d during times I through IV with no discernible difference amongst the planting treatments. Wetland cells with P1 were more efficient at removing NH4-N (1.1 g/m2/d) than P2 (0.6 g/m2/d) or WO (0.5 g/m2/d) at time I with differences decreasing by time IV (0.3 to 0.7 g/m2/d). During the winter (times V and VI), there were no differences in NH4-N removal amongst planting treatments with an average removal rate of 0.35 g/m2/d. There was a seasonal change in NH4-N removal in all the treatments, with the change most noticeable in the planted cells. The removal of NH4-N in WO was speculated to be due to a combination of sorption onto gravel, microbial assimilation, and nitrification at the air-water interface. The extra NH4-N removal in the planted cells diminished in the winter because the removal was most likely due to a combination of enhanced nitrification from O2 transport and NH4-N uptake mediated by seasonal macrophyte growth.

Research for Treating Avermectin Wastewater with UASB Technique

2011 ◽  
Vol 356-360 ◽  
pp. 1281-1284
Author(s):  
Yan Hong Chang ◽  
Hui Tao Feng ◽  
Hui Luo ◽  
San Jian Ma
Keyword(s):  
Retention Time ◽  
Hydraulic Retention Time ◽  
Removal Rate ◽  
Nitrogen Concentration ◽  
Ammonia Nitrogen ◽  
Cod Removal ◽  
Stable Phase ◽  
Volume Load ◽  
Cod Removal Rate

The avermectin wastewater was treated with UASB technique. The paper was focused mainly on the removal rate of COD and the change of ammonia nitrogen of influent and effluent wastewater in the first running stage. At the stable phase of anaerobic operation, the removal rate of COD could be stabilized at 85% when the influent volume load was 9.21 kg/(m3•d), and the effluent COD was about 1400 mg/L. As for ammonia nitrogen concentration of influent and effluent wastewater, in the first 50 days, the former was larger than the latter, after then, it was opposite. In the condition of same volume load but different hydraulic retention time (COD concentration of influent being different), COD removal rate kept almost the same. In the second running stage, the influent COD volume load reached 9.21 kg/(m3•d) at the 16th day, with the COD removal rate being around 87%.

Sign in / Sign up

Export Citation Format

Share Document